Optimized release of recombinant proteins by ultrasonication of E. coli cells

The release kinetics of β‐galactosidase protein have been determined during small‐scale ultrasonication of E. coli cells. Among several studied parameters, ionic strength and cell concentration have the least influence on the rate of protein recovery, whereas sample volume and acoustic power dramati...

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Veröffentlicht in:	Biotechnology and bioengineering 1998-06, Vol.58 (5), p.536-540
Hauptverfasser:	Feliu, J. X., Cubarsi, R., Villaverde, A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria beta-Galactosidase - biosynthesis beta-Galactosidase - isolation & purification Biological and medical sciences Biotechnology cell disruption Cloning, Molecular E. coli Enzyme kinetics Escherichia coli - drug effects Escherichia coli - genetics Escherichia coli - growth & development Fundamental and applied biological sciences. Psychology fusion proteins Growth kinetics Ionic strength Isopropyl Thiogalactoside - pharmacology Kinetics Methods. Procedures. Technologies Others Proteins Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - isolation & purification Recombinant Proteins - biosynthesis Recombinant Proteins - isolation & purification ultrasonication Ultrasonics Various methods and equipments β-galactosidase
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