Inulase-secreting strain of Saccharomyces cerevisiae produces fructose

The gene encoding inulase of the yeast Kluyveromyces marxianus (INU1Km) was cloned and expressed in the inulin-negative yeast Saccharomyces cerevisiae. Cells of S. cerevisiae transformed with the INU1km gene have acquired extracellular inulase activity and were able to grow in the medium with inulin...

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Veröffentlicht in:	Biotechnology and bioengineering 1998-11, Vol.60 (4), p.492-497
Hauptverfasser:	Brevnova, E.E. (Institute of Genetics and Selection of Industrial Microorganisms, Moscow, Russia.), Kozlov, D.G, Efremov, B.D, Benevolensky, S.V
Format:	Artikel
Sprache:	eng
Schlagworte:	Catalyst activity Cloning, Molecular DNA, Fungal - chemistry Enzyme kinetics FRUCTOSA FRUCTOSE Fructose - biosynthesis Genotype Glycoside Hydrolases - metabolism Glycoside Hydrolases - secretion Growth kinetics hexokinases Hydrogen-Ion Concentration inulase inulin Inulin - metabolism Kluyveromyces marxianus Restriction Mapping SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - enzymology secretion Temperature Yeast
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