Nucleation and growth of microbial lipase crystals from clarified concentrated fermentation broths[]

Bulk crystallization is emerging as a new industrial operation for protein recovery. Characterization of bulk protein crystallization is more complex than protein crystallization for structural study where single crystals are grown in flow cells. This is because both nucleation and crystal growth pr...

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Veröffentlicht in:	Biotechnology and bioengineering 1998-03, Vol.57 (6), p.666-675
Hauptverfasser:	Jacobsen, C. (University College London, London, UK.), Garside, J, Hoare, M
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Bioreactors Biotechnology Cells Crystal growth Crystal structure CRYSTALLIZATION Electrophoresis, Polyacrylamide Gel - methods Fermentation Fundamental and applied biological sciences. Psychology Fungal Proteins - chemistry Fungal Proteins - metabolism Kinetics Lipase - chemistry Lipase - metabolism Methods. Procedures. Technologies Models, Chemical Nucleation Others pH effects protein PROTEIN CRYSTALLIZATION PURIFICATION recovery Single crystals Solubility TRIACYLGLYCEROL LIPASE Various methods and equipments
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