Apoptosis in fresh and cryopreserved cardiac valves of pig samples
To analyse the influence of cold ischemic time (CIT) (2–24 h) and of cryopreservation (liquid phase) on the viability of the valvular fibroblasts and in the presence of apoptosis. Cardiac valves from 10 pigs were evaluated by anatomo-pathological study of the wall, muscle and leaflet. At the same ti...
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| Veröffentlicht in: | Cell and tissue banking 2008-06, Vol.9 (2), p.101-107 |
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| creator | Rendal Vázquez, M. Esther Díaz Román, T. M. Rodríguez Cabarcos, M. Zavanella Botta, C. Domenech García, N. González Cuesta, M. Sánchez Dopico, M. J. Pértega Díaz, S. Andión Núñez, C. |
| description | To analyse the influence of cold ischemic time (CIT) (2–24 h) and of cryopreservation (liquid phase) on the viability of the valvular fibroblasts and in the presence of apoptosis. Cardiac valves from 10 pigs were evaluated by anatomo-pathological study of the wall, muscle and leaflet. At the same time, the presence of cellular death due to apoptosis was investigated in two ways; directly on tissue by Apodetec system and by two-colour flow cytometry assay analyzing a suspension of fibroblast from valve leaflets using Anexina V and propidium iodure (PI). We established three groups of samples to compare different experimental conditions: 2 h of ischemia (group 1), 24 h of ischemia (group 2), and a programme of cryopreservation (−1°C/min) after 2 h of ischemia, followed by storage in liquid nitrogen during a week and thawing was performed (group 3). The analysis of viabilities showed slight differences between all three groups. The results indicated CIT of 24 h undergoing more structural affectation than CIT of 2 h. Flow cytometry analysis did not show important differences between groups; however cryopreserved samples (group 3) slightly less viability and a higher percentage of death by apoptosis than group 1 and 2 using flow cytometry. Apoptosis was confirmed on tissue from all valves but mainly in samples of group 2 and group 3. In summary, the viability of the valves in the case of ischemic times of 2 h, 24 h or after cryopreservation/thawing differs slightly. The death of the cells is mainly mediated by necrosis and not by apoptosis. |
| doi_str_mv | 10.1007/s10561-008-9063-6 |
| format | Article |
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Esther ; Díaz Román, T. M. ; Rodríguez Cabarcos, M. ; Zavanella Botta, C. ; Domenech García, N. ; González Cuesta, M. ; Sánchez Dopico, M. J. ; Pértega Díaz, S. ; Andión Núñez, C.</creator><creatorcontrib>Rendal Vázquez, M. Esther ; Díaz Román, T. M. ; Rodríguez Cabarcos, M. ; Zavanella Botta, C. ; Domenech García, N. ; González Cuesta, M. ; Sánchez Dopico, M. J. ; Pértega Díaz, S. ; Andión Núñez, C.</creatorcontrib><description>To analyse the influence of cold ischemic time (CIT) (2–24 h) and of cryopreservation (liquid phase) on the viability of the valvular fibroblasts and in the presence of apoptosis. Cardiac valves from 10 pigs were evaluated by anatomo-pathological study of the wall, muscle and leaflet. At the same time, the presence of cellular death due to apoptosis was investigated in two ways; directly on tissue by Apodetec system and by two-colour flow cytometry assay analyzing a suspension of fibroblast from valve leaflets using Anexina V and propidium iodure (PI). We established three groups of samples to compare different experimental conditions: 2 h of ischemia (group 1), 24 h of ischemia (group 2), and a programme of cryopreservation (−1°C/min) after 2 h of ischemia, followed by storage in liquid nitrogen during a week and thawing was performed (group 3). The analysis of viabilities showed slight differences between all three groups. The results indicated CIT of 24 h undergoing more structural affectation than CIT of 2 h. Flow cytometry analysis did not show important differences between groups; however cryopreserved samples (group 3) slightly less viability and a higher percentage of death by apoptosis than group 1 and 2 using flow cytometry. Apoptosis was confirmed on tissue from all valves but mainly in samples of group 2 and group 3. In summary, the viability of the valves in the case of ischemic times of 2 h, 24 h or after cryopreservation/thawing differs slightly. The death of the cells is mainly mediated by necrosis and not by apoptosis.</description><identifier>ISSN: 1389-9333</identifier><identifier>EISSN: 1573-6814</identifier><identifier>DOI: 10.1007/s10561-008-9063-6</identifier><identifier>PMID: 18320355</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ; Animals ; Apoptosis ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedicine ; Bone marrow, stem cells transplantation. Graft versus host reaction ; Cell Biology ; Cell Survival ; Cold Ischemia ; Comparative studies ; Cryogenic engineering ; Cryopreservation - methods ; Fibroblasts - cytology ; Fibroblasts - physiology ; Heart ; Heart Valves - cytology ; Heart Valves - physiology ; Hogs ; Life Sciences ; Medical sciences ; Necrosis ; Organ Preservation - methods ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Swine ; Tissue Banks ; Tissue Survival ; Tissues ; Transfusions. Complications. Transfusion reactions. Cell and gene therapy ; Transplant Surgery</subject><ispartof>Cell and tissue banking, 2008-06, Vol.9 (2), p.101-107</ispartof><rights>Springer Science+Business Media B.V. 2008</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c461t-e12b1a2cd8985c8b1e6f8dbd1631fe0b1d4ac9e880968e8c8b4caa3f24fc14f3</citedby><cites>FETCH-LOGICAL-c461t-e12b1a2cd8985c8b1e6f8dbd1631fe0b1d4ac9e880968e8c8b4caa3f24fc14f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10561-008-9063-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10561-008-9063-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20349030$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18320355$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rendal Vázquez, M. Esther</creatorcontrib><creatorcontrib>Díaz Román, T. M.</creatorcontrib><creatorcontrib>Rodríguez Cabarcos, M.</creatorcontrib><creatorcontrib>Zavanella Botta, C.</creatorcontrib><creatorcontrib>Domenech García, N.</creatorcontrib><creatorcontrib>González Cuesta, M.</creatorcontrib><creatorcontrib>Sánchez Dopico, M. J.</creatorcontrib><creatorcontrib>Pértega Díaz, S.</creatorcontrib><creatorcontrib>Andión Núñez, C.</creatorcontrib><title>Apoptosis in fresh and cryopreserved cardiac valves of pig samples</title><title>Cell and tissue banking</title><addtitle>Cell Tissue Banking</addtitle><addtitle>Cell Tissue Bank</addtitle><description>To analyse the influence of cold ischemic time (CIT) (2–24 h) and of cryopreservation (liquid phase) on the viability of the valvular fibroblasts and in the presence of apoptosis. Cardiac valves from 10 pigs were evaluated by anatomo-pathological study of the wall, muscle and leaflet. At the same time, the presence of cellular death due to apoptosis was investigated in two ways; directly on tissue by Apodetec system and by two-colour flow cytometry assay analyzing a suspension of fibroblast from valve leaflets using Anexina V and propidium iodure (PI). We established three groups of samples to compare different experimental conditions: 2 h of ischemia (group 1), 24 h of ischemia (group 2), and a programme of cryopreservation (−1°C/min) after 2 h of ischemia, followed by storage in liquid nitrogen during a week and thawing was performed (group 3). The analysis of viabilities showed slight differences between all three groups. The results indicated CIT of 24 h undergoing more structural affectation than CIT of 2 h. Flow cytometry analysis did not show important differences between groups; however cryopreserved samples (group 3) slightly less viability and a higher percentage of death by apoptosis than group 1 and 2 using flow cytometry. Apoptosis was confirmed on tissue from all valves but mainly in samples of group 2 and group 3. In summary, the viability of the valves in the case of ischemic times of 2 h, 24 h or after cryopreservation/thawing differs slightly. The death of the cells is mainly mediated by necrosis and not by apoptosis.</description><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Bone marrow, stem cells transplantation. Graft versus host reaction</subject><subject>Cell Biology</subject><subject>Cell Survival</subject><subject>Cold Ischemia</subject><subject>Comparative studies</subject><subject>Cryogenic engineering</subject><subject>Cryopreservation - methods</subject><subject>Fibroblasts - cytology</subject><subject>Fibroblasts - physiology</subject><subject>Heart</subject><subject>Heart Valves - cytology</subject><subject>Heart Valves - physiology</subject><subject>Hogs</subject><subject>Life Sciences</subject><subject>Medical sciences</subject><subject>Necrosis</subject><subject>Organ Preservation - methods</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Swine</subject><subject>Tissue Banks</subject><subject>Tissue Survival</subject><subject>Tissues</subject><subject>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</subject><subject>Transplant Surgery</subject><issn>1389-9333</issn><issn>1573-6814</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkd1LHDEQwENRqlX_gL6URbBvq5kkm5s8qtgPEHzxPWSzE7uyt7tm7g7875vjjgqFYp8yQ37zkfyE-AzyEqRcXDHIxkItJdZOWl3bD-IYmkUJEMxBiTW62mmtj8Qn5mcplVwo_VEcAWolddMci5vreZpXE_dc9WOVMvGvKoxdFfPrNJeM8oZKFnLXh1htwrAhrqZUzf1TxWE5D8Sn4jCFgelsf56Ix293j7c_6vuH7z9vr-_raCysagLVQlCxQ4dNxBbIJuzaDqyGRLKFzoToCFE6i4SFMDEEnZRJEUzSJ-Lrru2cp5c18cove440DGGkac3eOkA0Dt4Ft59gnVL_BTboTAHP_wKfp3Uey2O9Au0Qrd2OhR0U88ScKfk598uQXz1Iv9Xld7p80eW3urwtNV_2jdftkrq3ir2fAlzsgcAxDCmHMfb8hyuQcVLLwqkdx-VqfKL8tuG_p_8GcOOr9w</recordid><startdate>20080601</startdate><enddate>20080601</enddate><creator>Rendal Vázquez, M. Esther</creator><creator>Díaz Román, T. 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Esther</au><au>Díaz Román, T. M.</au><au>Rodríguez Cabarcos, M.</au><au>Zavanella Botta, C.</au><au>Domenech García, N.</au><au>González Cuesta, M.</au><au>Sánchez Dopico, M. J.</au><au>Pértega Díaz, S.</au><au>Andión Núñez, C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Apoptosis in fresh and cryopreserved cardiac valves of pig samples</atitle><jtitle>Cell and tissue banking</jtitle><stitle>Cell Tissue Banking</stitle><addtitle>Cell Tissue Bank</addtitle><date>2008-06-01</date><risdate>2008</risdate><volume>9</volume><issue>2</issue><spage>101</spage><epage>107</epage><pages>101-107</pages><issn>1389-9333</issn><eissn>1573-6814</eissn><abstract>To analyse the influence of cold ischemic time (CIT) (2–24 h) and of cryopreservation (liquid phase) on the viability of the valvular fibroblasts and in the presence of apoptosis. Cardiac valves from 10 pigs were evaluated by anatomo-pathological study of the wall, muscle and leaflet. At the same time, the presence of cellular death due to apoptosis was investigated in two ways; directly on tissue by Apodetec system and by two-colour flow cytometry assay analyzing a suspension of fibroblast from valve leaflets using Anexina V and propidium iodure (PI). We established three groups of samples to compare different experimental conditions: 2 h of ischemia (group 1), 24 h of ischemia (group 2), and a programme of cryopreservation (−1°C/min) after 2 h of ischemia, followed by storage in liquid nitrogen during a week and thawing was performed (group 3). The analysis of viabilities showed slight differences between all three groups. The results indicated CIT of 24 h undergoing more structural affectation than CIT of 2 h. Flow cytometry analysis did not show important differences between groups; however cryopreserved samples (group 3) slightly less viability and a higher percentage of death by apoptosis than group 1 and 2 using flow cytometry. Apoptosis was confirmed on tissue from all valves but mainly in samples of group 2 and group 3. In summary, the viability of the valves in the case of ischemic times of 2 h, 24 h or after cryopreservation/thawing differs slightly. The death of the cells is mainly mediated by necrosis and not by apoptosis.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>18320355</pmid><doi>10.1007/s10561-008-9063-6</doi><tpages>7</tpages></addata></record> |
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| subjects | Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Animals Apoptosis Biological and medical sciences Biomedical and Life Sciences Biomedicine Bone marrow, stem cells transplantation. Graft versus host reaction Cell Biology Cell Survival Cold Ischemia Comparative studies Cryogenic engineering Cryopreservation - methods Fibroblasts - cytology Fibroblasts - physiology Heart Heart Valves - cytology Heart Valves - physiology Hogs Life Sciences Medical sciences Necrosis Organ Preservation - methods Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Swine Tissue Banks Tissue Survival Tissues Transfusions. Complications. Transfusion reactions. Cell and gene therapy Transplant Surgery |
| title | Apoptosis in fresh and cryopreserved cardiac valves of pig samples |
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