Glutamate/aspartate and leu-enkephalin immunoreactivity in mammillothalamic projection neurons of the rat
We have used retrograde transport and immunohistochemistry to study glutamate, aspartate, and enkephalin-like immunoreactive pathways from the mammillary nuclei to the anterior nuclei of the thalamus. Injections of wheat germ agglutinin conjugated to horseradish peroxidase into the anterodorsal thal...
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| Veröffentlicht in: | Brain research bulletin 1998-12, Vol.47 (6), p.565-574 |
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| creator | Gonzalo-Ruiz, A Morte, L Sanz, J.M |
| description | We have used retrograde transport and immunohistochemistry to study glutamate, aspartate, and enkephalin-like immunoreactive pathways from the mammillary nuclei to the anterior nuclei of the thalamus. Injections of wheat germ agglutinin conjugated to horseradish peroxidase into the anterodorsal thalamic nucleus resulted in retrogradely labelled cell bodies in the lateral mammillary nucleus, bilaterally, whereas injections into the anteroventral thalamic nucleus resulted in retrogradely labelled neurons in the ipsilateral medial mammillary nucleus. In three parallel series of sections immunoreacted for glutamate, aspartate, and enkephalin, respectively, 50–60% of the retrogradely labelled cell bodies were also immunolabelled for glutamate, 50–60% for aspartate, and 40–50% for enkephalin. The enkephalin-immunoreactive neurons may coincide with or constitute a separate population from the glutamate/aspartate-containing neurons. These results are compatible with the possibility that mammillothalamic projection neurons may use glutamate and/or aspartate and enkephalin as neurotransmitters. |
| doi_str_mv | 10.1016/S0361-9230(98)00077-X |
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Injections of wheat germ agglutinin conjugated to horseradish peroxidase into the anterodorsal thalamic nucleus resulted in retrogradely labelled cell bodies in the lateral mammillary nucleus, bilaterally, whereas injections into the anteroventral thalamic nucleus resulted in retrogradely labelled neurons in the ipsilateral medial mammillary nucleus. In three parallel series of sections immunoreacted for glutamate, aspartate, and enkephalin, respectively, 50–60% of the retrogradely labelled cell bodies were also immunolabelled for glutamate, 50–60% for aspartate, and 40–50% for enkephalin. The enkephalin-immunoreactive neurons may coincide with or constitute a separate population from the glutamate/aspartate-containing neurons. These results are compatible with the possibility that mammillothalamic projection neurons may use glutamate and/or aspartate and enkephalin as neurotransmitters.</description><identifier>ISSN: 0361-9230</identifier><identifier>EISSN: 1873-2747</identifier><identifier>DOI: 10.1016/S0361-9230(98)00077-X</identifier><identifier>PMID: 10078614</identifier><identifier>CODEN: BRBUDU</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Animals ; Anterior thalamic nuclei ; Aspartic Acid - analysis ; Axons - chemistry ; Biological and medical sciences ; Central nervous system ; Central neurotransmission. Neuromudulation. Pathways and receptors ; Enkephalin, Leucine - analysis ; Excitatory amino acids ; Female ; Fundamental and applied biological sciences. Psychology ; Glutamic Acid - analysis ; Limbic system ; Limbic System - chemistry ; Limbic System - cytology ; Limbic System - physiology ; Male ; Mammillary Bodies - chemistry ; Mammillary Bodies - cytology ; Mammillary Bodies - physiology ; Mammillary nuclei ; Microinjections ; Neurons - chemistry ; Neurons - physiology ; Neurons - ultrastructure ; Neuropeptides ; Rats ; Rats, Wistar ; Retrograde tracing ; Thalamic Nuclei - chemistry ; Thalamic Nuclei - cytology ; Thalamic Nuclei - physiology ; Vertebrates: nervous system and sense organs ; Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate</subject><ispartof>Brain research bulletin, 1998-12, Vol.47 (6), p.565-574</ispartof><rights>1999 Elsevier Science Inc.</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-15d0a111c4e18026eae793a3f540d6bd223472c2f19d2c1345d0a5173ea848513</citedby><cites>FETCH-LOGICAL-c442t-15d0a111c4e18026eae793a3f540d6bd223472c2f19d2c1345d0a5173ea848513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0361-9230(98)00077-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1745606$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10078614$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gonzalo-Ruiz, A</creatorcontrib><creatorcontrib>Morte, L</creatorcontrib><creatorcontrib>Sanz, J.M</creatorcontrib><title>Glutamate/aspartate and leu-enkephalin immunoreactivity in mammillothalamic projection neurons of the rat</title><title>Brain research bulletin</title><addtitle>Brain Res Bull</addtitle><description>We have used retrograde transport and immunohistochemistry to study glutamate, aspartate, and enkephalin-like immunoreactive pathways from the mammillary nuclei to the anterior nuclei of the thalamus. Injections of wheat germ agglutinin conjugated to horseradish peroxidase into the anterodorsal thalamic nucleus resulted in retrogradely labelled cell bodies in the lateral mammillary nucleus, bilaterally, whereas injections into the anteroventral thalamic nucleus resulted in retrogradely labelled neurons in the ipsilateral medial mammillary nucleus. In three parallel series of sections immunoreacted for glutamate, aspartate, and enkephalin, respectively, 50–60% of the retrogradely labelled cell bodies were also immunolabelled for glutamate, 50–60% for aspartate, and 40–50% for enkephalin. The enkephalin-immunoreactive neurons may coincide with or constitute a separate population from the glutamate/aspartate-containing neurons. These results are compatible with the possibility that mammillothalamic projection neurons may use glutamate and/or aspartate and enkephalin as neurotransmitters.</description><subject>Animals</subject><subject>Anterior thalamic nuclei</subject><subject>Aspartic Acid - analysis</subject><subject>Axons - chemistry</subject><subject>Biological and medical sciences</subject><subject>Central nervous system</subject><subject>Central neurotransmission. Neuromudulation. Pathways and receptors</subject><subject>Enkephalin, Leucine - analysis</subject><subject>Excitatory amino acids</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glutamic Acid - analysis</subject><subject>Limbic system</subject><subject>Limbic System - chemistry</subject><subject>Limbic System - cytology</subject><subject>Limbic System - physiology</subject><subject>Male</subject><subject>Mammillary Bodies - chemistry</subject><subject>Mammillary Bodies - cytology</subject><subject>Mammillary Bodies - physiology</subject><subject>Mammillary nuclei</subject><subject>Microinjections</subject><subject>Neurons - chemistry</subject><subject>Neurons - physiology</subject><subject>Neurons - ultrastructure</subject><subject>Neuropeptides</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Retrograde tracing</subject><subject>Thalamic Nuclei - chemistry</subject><subject>Thalamic Nuclei - cytology</subject><subject>Thalamic Nuclei - physiology</subject><subject>Vertebrates: nervous system and sense organs</subject><subject>Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate</subject><issn>0361-9230</issn><issn>1873-2747</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0E1rFTEUgOEgir2t_gQlC5G6GJuvycysihStQsGFCt2F08wZmpqPa5Ip9N-b23tRd64SwpPk8BLyirP3nHF99o1JzbtJSHY6je8YY8PQXT8hGz4OshODGp6SzR9yRI5LuWtIj71-To5446PmakPcpV8rBKh4BmULubYdhThTj2uH8Sdub8G7SF0Ia0wZwVZ37-oDbWcBQnDep9oIBGfpNqc7bCBFGnHNKRaaFlpvkWaoL8izBXzBl4f1hPz49PH7xefu6uvll4sPV51VStSO9zMDzrlVyEcmNAIOkwS59IrN-mYWQqpBWLHwaRaWS7XzPR8kwqjGnssT8nb_bpvm14qlmuCKRe8hYlqL0RNvEZRqsN9Dm1MpGRezzS5AfjCcmV1j89jY7AKaaTSPjc11u_f68MF6E3D-59Y-agNvDgCKBb9kiNaVv25QvWa6sfM9w1bj3mE2xTqMFmeXW0UzJ_efSX4Dap6aFg</recordid><startdate>19981201</startdate><enddate>19981201</enddate><creator>Gonzalo-Ruiz, A</creator><creator>Morte, L</creator><creator>Sanz, J.M</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19981201</creationdate><title>Glutamate/aspartate and leu-enkephalin immunoreactivity in mammillothalamic projection neurons of the rat</title><author>Gonzalo-Ruiz, A ; Morte, L ; Sanz, J.M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-15d0a111c4e18026eae793a3f540d6bd223472c2f19d2c1345d0a5173ea848513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Anterior thalamic nuclei</topic><topic>Aspartic Acid - analysis</topic><topic>Axons - chemistry</topic><topic>Biological and medical sciences</topic><topic>Central nervous system</topic><topic>Central neurotransmission. Neuromudulation. Pathways and receptors</topic><topic>Enkephalin, Leucine - analysis</topic><topic>Excitatory amino acids</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glutamic Acid - analysis</topic><topic>Limbic system</topic><topic>Limbic System - chemistry</topic><topic>Limbic System - cytology</topic><topic>Limbic System - physiology</topic><topic>Male</topic><topic>Mammillary Bodies - chemistry</topic><topic>Mammillary Bodies - cytology</topic><topic>Mammillary Bodies - physiology</topic><topic>Mammillary nuclei</topic><topic>Microinjections</topic><topic>Neurons - chemistry</topic><topic>Neurons - physiology</topic><topic>Neurons - ultrastructure</topic><topic>Neuropeptides</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Retrograde tracing</topic><topic>Thalamic Nuclei - chemistry</topic><topic>Thalamic Nuclei - cytology</topic><topic>Thalamic Nuclei - physiology</topic><topic>Vertebrates: nervous system and sense organs</topic><topic>Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gonzalo-Ruiz, A</creatorcontrib><creatorcontrib>Morte, L</creatorcontrib><creatorcontrib>Sanz, J.M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gonzalo-Ruiz, A</au><au>Morte, L</au><au>Sanz, J.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Glutamate/aspartate and leu-enkephalin immunoreactivity in mammillothalamic projection neurons of the rat</atitle><jtitle>Brain research bulletin</jtitle><addtitle>Brain Res Bull</addtitle><date>1998-12-01</date><risdate>1998</risdate><volume>47</volume><issue>6</issue><spage>565</spage><epage>574</epage><pages>565-574</pages><issn>0361-9230</issn><eissn>1873-2747</eissn><coden>BRBUDU</coden><abstract>We have used retrograde transport and immunohistochemistry to study glutamate, aspartate, and enkephalin-like immunoreactive pathways from the mammillary nuclei to the anterior nuclei of the thalamus. Injections of wheat germ agglutinin conjugated to horseradish peroxidase into the anterodorsal thalamic nucleus resulted in retrogradely labelled cell bodies in the lateral mammillary nucleus, bilaterally, whereas injections into the anteroventral thalamic nucleus resulted in retrogradely labelled neurons in the ipsilateral medial mammillary nucleus. In three parallel series of sections immunoreacted for glutamate, aspartate, and enkephalin, respectively, 50–60% of the retrogradely labelled cell bodies were also immunolabelled for glutamate, 50–60% for aspartate, and 40–50% for enkephalin. The enkephalin-immunoreactive neurons may coincide with or constitute a separate population from the glutamate/aspartate-containing neurons. These results are compatible with the possibility that mammillothalamic projection neurons may use glutamate and/or aspartate and enkephalin as neurotransmitters.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>10078614</pmid><doi>10.1016/S0361-9230(98)00077-X</doi><tpages>10</tpages></addata></record> |
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| subjects | Animals Anterior thalamic nuclei Aspartic Acid - analysis Axons - chemistry Biological and medical sciences Central nervous system Central neurotransmission. Neuromudulation. Pathways and receptors Enkephalin, Leucine - analysis Excitatory amino acids Female Fundamental and applied biological sciences. Psychology Glutamic Acid - analysis Limbic system Limbic System - chemistry Limbic System - cytology Limbic System - physiology Male Mammillary Bodies - chemistry Mammillary Bodies - cytology Mammillary Bodies - physiology Mammillary nuclei Microinjections Neurons - chemistry Neurons - physiology Neurons - ultrastructure Neuropeptides Rats Rats, Wistar Retrograde tracing Thalamic Nuclei - chemistry Thalamic Nuclei - cytology Thalamic Nuclei - physiology Vertebrates: nervous system and sense organs Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate |
| title | Glutamate/aspartate and leu-enkephalin immunoreactivity in mammillothalamic projection neurons of the rat |
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