Ig L-chain Shuffling for Affinity Maturation of Phage Library-derived Human Anti-human MCP-1 Antibody Blocking its Chemotactic Activity

Monocyte chemotactic protein-1 (MCP-1, CC-chemokine ligand 2; CCL2) is involved in the development of various forms of chronic inflammations. Employing the naive human single-chain Fv displaying phage library, we established seven MCP-1-specific scFvs. The MC8 and MC32 clones exhibited blocking acti...

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Veröffentlicht in:	Journal of biochemistry (Tokyo) 2008-05, Vol.143 (5), p.593-601
Hauptverfasser:	Yoshinaga, Keisuke, Matsumoto, Miyuki, Torikai, Masaharu, Sugyo, Kazuki, Kuroki, Saori, Nogami, Kentaro, Matsumoto, Ryo, Hashiguchi, Shuhei, Ito, Yuji, Nakashima, Toshihiro, Sugimura, Kazuhisa
Format:	Artikel
Sprache:	eng
Schlagworte:	affinity maturation Amino Acid Sequence Antibodies, Monoclonal - genetics Antibodies, Monoclonal - immunology Antibodies, Monoclonal - pharmacology Antibody Affinity Chemokine CCL2 - antagonists & inhibitors Chemokine CCL2 - immunology Chemotaxis - drug effects DNA Shuffling human antibody Humans Immunoglobulin G - immunology Immunoglobulin kappa-Chains - chemistry Immunoglobulin kappa-Chains - genetics Immunoglobulin kappa-Chains - immunology Immunoglobulin Variable Region - chemistry Immunoglobulin Variable Region - genetics Immunoglobulin Variable Region - immunology L-chain shuffling MCP-1 Molecular Sequence Data Peptide Library phage display library Sequence Homology, Amino Acid Surface Plasmon Resonance
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