Expression and purification of Mycobacterium tuberculosis ESAT-6 and MPT64 fusion protein and its immunoprophylactic potential in mouse model

The completion of Mycobacterium tuberculosis genome sequence has opened a new way for the identification and characterization of bacterial antigens, such as ESAT-6, CFP10, MPT64, and Ag85 complex, which are helpful for tuberculosis control. In this work, genes of ESAT-6 and MPT64 were fused and expr...

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Veröffentlicht in:	Protein expression and purification 2008-06, Vol.59 (2), p.189-196
Hauptverfasser:	Bai, Yinlan, Xue, Ying, Gao, Hui, Wang, Limei, Ding, Tianbing, Bai, Wentao, Fan, Ailin, Zhang, Jianfang, An, Qunxing, Xu, Zhikai
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antigens, Bacterial - biosynthesis Antigens, Bacterial - isolation & purification Antigens, Bacterial - therapeutic use Bacterial Proteins - biosynthesis Bacterial Proteins - isolation & purification Bacterial Proteins - therapeutic use Blotting, Western Chromatography, Affinity Cloning, Molecular Disease Models, Animal ESAT-6 Escherichia coli Escherichia coli - genetics Expression Genetic Vectors Humans Interferon-gamma - metabolism Interleukin-12 - metabolism Lymphocyte Activation Mice Mice, Inbred C57BL MPT64 Mycobacterium tuberculosis Mycobacterium tuberculosis - immunology Mycobacterium tuberculosis - isolation & purification Nitrilotriacetic Acid - chemistry Protein Folding Recombinant Proteins - biosynthesis Recombinant Proteins - isolation & purification Recombinant Proteins - therapeutic use Tuberculosis - immunology Tuberculosis - prevention & control Tuberculosis Vaccines - biosynthesis Tuberculosis Vaccines - isolation & purification Tuberculosis Vaccines - therapeutic use Vaccine
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