Gene cloning and expression of cytosolic glutathione reductase in rice (Oryza sativa L.)
We have isolated a cDNA (RGRC2) encoding glutathione reductase (GR) from rice (Oryza saliva L.). The comparison of deduced amino acid sequences from RGRC2 and other plant GR cDNAs indicated that RGRC2 encodes a putative cytosolic isoform. The recombinant RGRC2 protein had enzymatic properties compar...
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| Veröffentlicht in: | Plant and cell physiology 1998-12, Vol.39 (12), p.1269-1280 |
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| creator | Kaminaka, H. (Kyoto Prefectural Univ. (Japan). Faculty of Agriculture) Morita, S Nakajima, M Masumura, T Tanaka, K |
| description | We have isolated a cDNA (RGRC2) encoding glutathione reductase (GR) from rice (Oryza saliva L.). The comparison of deduced amino acid sequences from RGRC2 and other plant GR cDNAs indicated that RGRC2 encodes a putative cytosolic isoform. The recombinant RGRC2 protein had enzymatic properties comparable to those of GR from rice embryo. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues. Southern blot analysis showed that the RGRC2 gene exists as a single copy gene. Here, we have also isolated a genomic clone completely corresponding to RGRC2. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. From the presence of two ABA-responsive elements in the 5'-flanking region of RGRC2, we examined the expression in rice seedlings treated with ABA and the ABA-related environmental stresses, chilling, drought and salinity. The expression of RGRC2 was strongly induced by all these treatments. We suggest that the expression of the rice cytosolic GR gene is regulated via ABA-mediated signal transduction pathway under environmental stresses |
| doi_str_mv | 10.1093/oxfordjournals.pcp.a029330 |
| format | Article |
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(Kyoto Prefectural Univ. (Japan). Faculty of Agriculture) ; Morita, S ; Nakajima, M ; Masumura, T ; Tanaka, K</creator><creatorcontrib>Kaminaka, H. (Kyoto Prefectural Univ. (Japan). Faculty of Agriculture) ; Morita, S ; Nakajima, M ; Masumura, T ; Tanaka, K</creatorcontrib><description>We have isolated a cDNA (RGRC2) encoding glutathione reductase (GR) from rice (Oryza saliva L.). The comparison of deduced amino acid sequences from RGRC2 and other plant GR cDNAs indicated that RGRC2 encodes a putative cytosolic isoform. The recombinant RGRC2 protein had enzymatic properties comparable to those of GR from rice embryo. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues. Southern blot analysis showed that the RGRC2 gene exists as a single copy gene. Here, we have also isolated a genomic clone completely corresponding to RGRC2. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. From the presence of two ABA-responsive elements in the 5'-flanking region of RGRC2, we examined the expression in rice seedlings treated with ABA and the ABA-related environmental stresses, chilling, drought and salinity. The expression of RGRC2 was strongly induced by all these treatments. We suggest that the expression of the rice cytosolic GR gene is regulated via ABA-mediated signal transduction pathway under environmental stresses</description><identifier>ISSN: 0032-0781</identifier><identifier>EISSN: 1471-9053</identifier><identifier>DOI: 10.1093/oxfordjournals.pcp.a029330</identifier><identifier>PMID: 10050312</identifier><identifier>CODEN: PCPHA5</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>5' Untranslated Regions ; Abscisic acid ; Abscisic Acid - pharmacology ; Agronomy. Soil science and plant productions ; Amino Acid Sequence ; Biological and medical sciences ; CITOPLASMA ; Classical and quantitative genetics. Population genetics. Molecular genetics ; CLONACION MOLECULAR ; CLONAGE MOLECULAIRE ; Cold Temperature ; CYTOPLASM ; CYTOPLASME ; Cytosol - enzymology ; Cytosolic isoform ; DNA, Complementary - genetics ; Escherichia coli - genetics ; Exons ; EXPRESION GENICA ; EXPRESSION DES GENES ; Fundamental and applied biological sciences. Psychology ; Gene Dosage ; GENE EXPRESSION ; Gene Expression Regulation, Plant ; Gene structure ; Generalities. Genetics. Plant material ; Genes, Plant ; Genes. Genome ; Genetics and breeding of economic plants ; GLUTATHION REDUCTASE ; GLUTATHIONE REDUCTASE ; Glutathione reductase (EC 1.6.4.2) ; Glutathione Reductase - genetics ; GLUTATION REDUCTASA ; Isoenzymes - genetics ; Molecular and cellular biology ; MOLECULAR CLONING ; Molecular genetics ; Molecular Sequence Data ; Oryza - enzymology ; Oryza - genetics ; ORYZA SATIVA ; Oryza sativa L ; Recombinant Proteins ; Seeds - enzymology ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; Stress response ; Tissue Distribution</subject><ispartof>Plant and cell physiology, 1998-12, Vol.39 (12), p.1269-1280</ispartof><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c512t-58170f8f76e86537bd94c85413beff4aa48841a0eba581c5d865d070b053ea9d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1717184$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10050312$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kaminaka, H. (Kyoto Prefectural Univ. (Japan). Faculty of Agriculture)</creatorcontrib><creatorcontrib>Morita, S</creatorcontrib><creatorcontrib>Nakajima, M</creatorcontrib><creatorcontrib>Masumura, T</creatorcontrib><creatorcontrib>Tanaka, K</creatorcontrib><title>Gene cloning and expression of cytosolic glutathione reductase in rice (Oryza sativa L.)</title><title>Plant and cell physiology</title><addtitle>Plant Cell Physiol</addtitle><description>We have isolated a cDNA (RGRC2) encoding glutathione reductase (GR) from rice (Oryza saliva L.). The comparison of deduced amino acid sequences from RGRC2 and other plant GR cDNAs indicated that RGRC2 encodes a putative cytosolic isoform. The recombinant RGRC2 protein had enzymatic properties comparable to those of GR from rice embryo. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues. Southern blot analysis showed that the RGRC2 gene exists as a single copy gene. Here, we have also isolated a genomic clone completely corresponding to RGRC2. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. From the presence of two ABA-responsive elements in the 5'-flanking region of RGRC2, we examined the expression in rice seedlings treated with ABA and the ABA-related environmental stresses, chilling, drought and salinity. The expression of RGRC2 was strongly induced by all these treatments. We suggest that the expression of the rice cytosolic GR gene is regulated via ABA-mediated signal transduction pathway under environmental stresses</description><subject>5' Untranslated Regions</subject><subject>Abscisic acid</subject><subject>Abscisic Acid - pharmacology</subject><subject>Agronomy. Soil science and plant productions</subject><subject>Amino Acid Sequence</subject><subject>Biological and medical sciences</subject><subject>CITOPLASMA</subject><subject>Classical and quantitative genetics. Population genetics. Molecular genetics</subject><subject>CLONACION MOLECULAR</subject><subject>CLONAGE MOLECULAIRE</subject><subject>Cold Temperature</subject><subject>CYTOPLASM</subject><subject>CYTOPLASME</subject><subject>Cytosol - enzymology</subject><subject>Cytosolic isoform</subject><subject>DNA, Complementary - genetics</subject><subject>Escherichia coli - genetics</subject><subject>Exons</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Dosage</subject><subject>GENE EXPRESSION</subject><subject>Gene Expression Regulation, Plant</subject><subject>Gene structure</subject><subject>Generalities. Genetics. Plant material</subject><subject>Genes, Plant</subject><subject>Genes. Genome</subject><subject>Genetics and breeding of economic plants</subject><subject>GLUTATHION REDUCTASE</subject><subject>GLUTATHIONE REDUCTASE</subject><subject>Glutathione reductase (EC 1.6.4.2)</subject><subject>Glutathione Reductase - genetics</subject><subject>GLUTATION REDUCTASA</subject><subject>Isoenzymes - genetics</subject><subject>Molecular and cellular biology</subject><subject>MOLECULAR CLONING</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Oryza - enzymology</subject><subject>Oryza - genetics</subject><subject>ORYZA SATIVA</subject><subject>Oryza sativa L</subject><subject>Recombinant Proteins</subject><subject>Seeds - enzymology</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><subject>Stress response</subject><subject>Tissue Distribution</subject><issn>0032-0781</issn><issn>1471-9053</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0ktv1DAQAOAIgei28BNAFkIVHLKM4yS2e4MCLWilVgKkVS_WxLEXl2wc7ATt8utxlRWPE_LBlv3N-DHOsmcUlhQke-V31of21k-hxy4uBz0sEQrJGNzLFrTkNJdQsfvZAoAVOXBBj7LjGG8B0pjBw-yIAlTAaLHI1hemN0R3vnf9hmDfErMbgonR-Z54S_R-9NF3TpNNN404fk3zhgTTTnrEaIjrSXDakBdXYf8TScTR_UCyWr58lD2w6XTm8aE_yb68f_f5_DJfXV18OH-9ynVFizGvBOVgheW1EXXFeNPKUouqpKwx1paIpRAlRTANJqqrNqkWODTphgZly06y0znvEPz3ycRRbV3UpuuwN36KqpaU14wX_4WUF8CkuINnM9TBxxiMVUNwWwx7RUHdFUD9WwCVCqAOBUjBTw-7TM3WtH-Fzi-ewPMDwKixswF77eIfx1MTZWL5zFwcze73MoZvquaMV-pyfaPWxfrT9VvxRt0k_2T2Fr3CTUgpP15TKWX6A3Ul2S8eA6zF</recordid><startdate>19981201</startdate><enddate>19981201</enddate><creator>Kaminaka, H. (Kyoto Prefectural Univ. (Japan). Faculty of Agriculture)</creator><creator>Morita, S</creator><creator>Nakajima, M</creator><creator>Masumura, T</creator><creator>Tanaka, K</creator><general>Oxford University Press</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19981201</creationdate><title>Gene cloning and expression of cytosolic glutathione reductase in rice (Oryza sativa L.)</title><author>Kaminaka, H. (Kyoto Prefectural Univ. (Japan). Faculty of Agriculture) ; Morita, S ; Nakajima, M ; Masumura, T ; Tanaka, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c512t-58170f8f76e86537bd94c85413beff4aa48841a0eba581c5d865d070b053ea9d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>5' Untranslated Regions</topic><topic>Abscisic acid</topic><topic>Abscisic Acid - pharmacology</topic><topic>Agronomy. Soil science and plant productions</topic><topic>Amino Acid Sequence</topic><topic>Biological and medical sciences</topic><topic>CITOPLASMA</topic><topic>Classical and quantitative genetics. Population genetics. Molecular genetics</topic><topic>CLONACION MOLECULAR</topic><topic>CLONAGE MOLECULAIRE</topic><topic>Cold Temperature</topic><topic>CYTOPLASM</topic><topic>CYTOPLASME</topic><topic>Cytosol - enzymology</topic><topic>Cytosolic isoform</topic><topic>DNA, Complementary - genetics</topic><topic>Escherichia coli - genetics</topic><topic>Exons</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Dosage</topic><topic>GENE EXPRESSION</topic><topic>Gene Expression Regulation, Plant</topic><topic>Gene structure</topic><topic>Generalities. Genetics. Plant material</topic><topic>Genes, Plant</topic><topic>Genes. Genome</topic><topic>Genetics and breeding of economic plants</topic><topic>GLUTATHION REDUCTASE</topic><topic>GLUTATHIONE REDUCTASE</topic><topic>Glutathione reductase (EC 1.6.4.2)</topic><topic>Glutathione Reductase - genetics</topic><topic>GLUTATION REDUCTASA</topic><topic>Isoenzymes - genetics</topic><topic>Molecular and cellular biology</topic><topic>MOLECULAR CLONING</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Oryza - enzymology</topic><topic>Oryza - genetics</topic><topic>ORYZA SATIVA</topic><topic>Oryza sativa L</topic><topic>Recombinant Proteins</topic><topic>Seeds - enzymology</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><topic>Stress response</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kaminaka, H. (Kyoto Prefectural Univ. (Japan). Faculty of Agriculture)</creatorcontrib><creatorcontrib>Morita, S</creatorcontrib><creatorcontrib>Nakajima, M</creatorcontrib><creatorcontrib>Masumura, T</creatorcontrib><creatorcontrib>Tanaka, K</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant and cell physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kaminaka, H. (Kyoto Prefectural Univ. (Japan). Faculty of Agriculture)</au><au>Morita, S</au><au>Nakajima, M</au><au>Masumura, T</au><au>Tanaka, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gene cloning and expression of cytosolic glutathione reductase in rice (Oryza sativa L.)</atitle><jtitle>Plant and cell physiology</jtitle><addtitle>Plant Cell Physiol</addtitle><date>1998-12-01</date><risdate>1998</risdate><volume>39</volume><issue>12</issue><spage>1269</spage><epage>1280</epage><pages>1269-1280</pages><issn>0032-0781</issn><eissn>1471-9053</eissn><coden>PCPHA5</coden><abstract>We have isolated a cDNA (RGRC2) encoding glutathione reductase (GR) from rice (Oryza saliva L.). The comparison of deduced amino acid sequences from RGRC2 and other plant GR cDNAs indicated that RGRC2 encodes a putative cytosolic isoform. The recombinant RGRC2 protein had enzymatic properties comparable to those of GR from rice embryo. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues. Southern blot analysis showed that the RGRC2 gene exists as a single copy gene. Here, we have also isolated a genomic clone completely corresponding to RGRC2. The RGRC2 gene is split into 16 exons spread about 7.4 kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. From the presence of two ABA-responsive elements in the 5'-flanking region of RGRC2, we examined the expression in rice seedlings treated with ABA and the ABA-related environmental stresses, chilling, drought and salinity. The expression of RGRC2 was strongly induced by all these treatments. We suggest that the expression of the rice cytosolic GR gene is regulated via ABA-mediated signal transduction pathway under environmental stresses</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>10050312</pmid><doi>10.1093/oxfordjournals.pcp.a029330</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Plant and cell physiology, 1998-12, Vol.39 (12), p.1269-1280 |
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| language | eng |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); Open Access Titles of Japan; EZB-FREE-00999 freely available EZB journals |
| subjects | 5' Untranslated Regions Abscisic acid Abscisic Acid - pharmacology Agronomy. Soil science and plant productions Amino Acid Sequence Biological and medical sciences CITOPLASMA Classical and quantitative genetics. Population genetics. Molecular genetics CLONACION MOLECULAR CLONAGE MOLECULAIRE Cold Temperature CYTOPLASM CYTOPLASME Cytosol - enzymology Cytosolic isoform DNA, Complementary - genetics Escherichia coli - genetics Exons EXPRESION GENICA EXPRESSION DES GENES Fundamental and applied biological sciences. Psychology Gene Dosage GENE EXPRESSION Gene Expression Regulation, Plant Gene structure Generalities. Genetics. Plant material Genes, Plant Genes. Genome Genetics and breeding of economic plants GLUTATHION REDUCTASE GLUTATHIONE REDUCTASE Glutathione reductase (EC 1.6.4.2) Glutathione Reductase - genetics GLUTATION REDUCTASA Isoenzymes - genetics Molecular and cellular biology MOLECULAR CLONING Molecular genetics Molecular Sequence Data Oryza - enzymology Oryza - genetics ORYZA SATIVA Oryza sativa L Recombinant Proteins Seeds - enzymology Sequence Analysis, DNA Sequence Homology, Amino Acid Stress response Tissue Distribution |
| title | Gene cloning and expression of cytosolic glutathione reductase in rice (Oryza sativa L.) |
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