HMGB1 protein inhibits DNA replication in vitro: A role of the acetylation and the acidic tail
The high mobility group box (HMGB) 1 protein is a very abundant and conserved protein that is implicated in many key cellular events but its functions within the nucleus remain elusive. The role of this protein in replication of closed circular DNA containing a eukaryotic origin of replication has b...
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| Veröffentlicht in: | The international journal of biochemistry & cell biology 2008, Vol.40 (8), p.1536-1542 |
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| container_issue | 8 |
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| container_title | The international journal of biochemistry & cell biology |
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| creator | Topalova, Dessislava Ugrinova, Iva Pashev, Iliya G. Pasheva, Evdokia A. |
| description | The high mobility group box (HMGB) 1 protein is a very abundant and conserved protein that is implicated in many key cellular events but its functions within the nucleus remain elusive. The role of this protein in replication of closed circular DNA containing a eukaryotic origin of replication has been studied in vitro by using native and recombinant HMGB1 as well as various modified HMGB1 preparations such as truncated protein, lacking its C-terminal tail, in vivo acetylated protein, and recombinant HMGB1 phosphorylated in vitro by protein kinase C (PKC). Native HMGB1 extracted from tumour cells inhibits replication and this effect is reduced upon acetylation and completely abolished upon removal of the acidic C-terminal tail. Recombinant HMGB1, however, fails to inhibit replication but it acquires such a property following in vitro phosphorylation by PKC. |
| doi_str_mv | 10.1016/j.biocel.2007.11.014 |
| format | Article |
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Recombinant HMGB1, however, fails to inhibit replication but it acquires such a property following in vitro phosphorylation by PKC.</description><identifier>ISSN: 1357-2725</identifier><identifier>EISSN: 1878-5875</identifier><identifier>DOI: 10.1016/j.biocel.2007.11.014</identifier><identifier>PMID: 18191612</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Acetylation ; Acidic tail ; Animals ; Aphidicolin - pharmacology ; DNA replication ; DNA Replication - drug effects ; DNA, Circular - metabolism ; High Mobility Group Proteins - pharmacology ; HMGB1 Protein ; Humans ; Phosphorylation ; Protein Kinase C - metabolism ; Rats ; Recombinant Proteins - pharmacology ; Repressor Proteins - pharmacology ; Tumor Cells, Cultured</subject><ispartof>The international journal of biochemistry & cell biology, 2008, Vol.40 (8), p.1536-1542</ispartof><rights>2007 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c360t-adf71ac6ee02a126fd970a6c6c2e5858b18a64c728c034415f974c87220adfe33</citedby><cites>FETCH-LOGICAL-c360t-adf71ac6ee02a126fd970a6c6c2e5858b18a64c728c034415f974c87220adfe33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.biocel.2007.11.014$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,4010,27904,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18191612$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Topalova, Dessislava</creatorcontrib><creatorcontrib>Ugrinova, Iva</creatorcontrib><creatorcontrib>Pashev, Iliya G.</creatorcontrib><creatorcontrib>Pasheva, Evdokia A.</creatorcontrib><title>HMGB1 protein inhibits DNA replication in vitro: A role of the acetylation and the acidic tail</title><title>The international journal of biochemistry & cell biology</title><addtitle>Int J Biochem Cell Biol</addtitle><description>The high mobility group box (HMGB) 1 protein is a very abundant and conserved protein that is implicated in many key cellular events but its functions within the nucleus remain elusive. The role of this protein in replication of closed circular DNA containing a eukaryotic origin of replication has been studied in vitro by using native and recombinant HMGB1 as well as various modified HMGB1 preparations such as truncated protein, lacking its C-terminal tail, in vivo acetylated protein, and recombinant HMGB1 phosphorylated in vitro by protein kinase C (PKC). Native HMGB1 extracted from tumour cells inhibits replication and this effect is reduced upon acetylation and completely abolished upon removal of the acidic C-terminal tail. Recombinant HMGB1, however, fails to inhibit replication but it acquires such a property following in vitro phosphorylation by PKC.</description><subject>Acetylation</subject><subject>Acidic tail</subject><subject>Animals</subject><subject>Aphidicolin - pharmacology</subject><subject>DNA replication</subject><subject>DNA Replication - drug effects</subject><subject>DNA, Circular - metabolism</subject><subject>High Mobility Group Proteins - pharmacology</subject><subject>HMGB1 Protein</subject><subject>Humans</subject><subject>Phosphorylation</subject><subject>Protein Kinase C - metabolism</subject><subject>Rats</subject><subject>Recombinant Proteins - pharmacology</subject><subject>Repressor Proteins - pharmacology</subject><subject>Tumor Cells, Cultured</subject><issn>1357-2725</issn><issn>1878-5875</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9P3DAQxS1ExZ-l3wAhn7gl9TiJ7eWARCmFSrS90GstrzMRs8rGW9uLxLevV1mpt55mNPPejN6PsUsQNQhQn9b1ioLHsZZC6BqgFtAesTMw2lSd0d1x6ZtOV1LL7pSdp7QWQkAnmxN2CgaWoECesd9P3x8_A9_GkJEmTtMrrSgn_uXHHY-4Hcm7TGG_4G-UY7jhZR5G5GHg-RW585jfx1njpv4wo548z47GC_ZhcGPCj4e6YL--PrzcP1XPPx-_3d89V75RIleuHzQ4rxCFdCDV0C-1cMorL7EznVmBcar1WhovmraFbljq1hstpShWbJoFu57vliB_dpiy3VAqcEY3Ydglq5agW13CL1g7C30MKUUc7DbSxsV3C8Luudq1nbnaPVcLYAvXYrs63N-tNtj_Mx1AFsHtLMCS8o0w2uQJJ489RfTZ9oH-_-EvdaGJ_Q</recordid><startdate>2008</startdate><enddate>2008</enddate><creator>Topalova, Dessislava</creator><creator>Ugrinova, Iva</creator><creator>Pashev, Iliya G.</creator><creator>Pasheva, Evdokia A.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2008</creationdate><title>HMGB1 protein inhibits DNA replication in vitro: A role of the acetylation and the acidic tail</title><author>Topalova, Dessislava ; Ugrinova, Iva ; Pashev, Iliya G. ; Pasheva, Evdokia A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-adf71ac6ee02a126fd970a6c6c2e5858b18a64c728c034415f974c87220adfe33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Acetylation</topic><topic>Acidic tail</topic><topic>Animals</topic><topic>Aphidicolin - pharmacology</topic><topic>DNA replication</topic><topic>DNA Replication - drug effects</topic><topic>DNA, Circular - metabolism</topic><topic>High Mobility Group Proteins - pharmacology</topic><topic>HMGB1 Protein</topic><topic>Humans</topic><topic>Phosphorylation</topic><topic>Protein Kinase C - metabolism</topic><topic>Rats</topic><topic>Recombinant Proteins - pharmacology</topic><topic>Repressor Proteins - pharmacology</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Topalova, Dessislava</creatorcontrib><creatorcontrib>Ugrinova, Iva</creatorcontrib><creatorcontrib>Pashev, Iliya G.</creatorcontrib><creatorcontrib>Pasheva, Evdokia A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The international journal of biochemistry & cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Topalova, Dessislava</au><au>Ugrinova, Iva</au><au>Pashev, Iliya G.</au><au>Pasheva, Evdokia A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>HMGB1 protein inhibits DNA replication in vitro: A role of the acetylation and the acidic tail</atitle><jtitle>The international journal of biochemistry & cell biology</jtitle><addtitle>Int J Biochem Cell Biol</addtitle><date>2008</date><risdate>2008</risdate><volume>40</volume><issue>8</issue><spage>1536</spage><epage>1542</epage><pages>1536-1542</pages><issn>1357-2725</issn><eissn>1878-5875</eissn><abstract>The high mobility group box (HMGB) 1 protein is a very abundant and conserved protein that is implicated in many key cellular events but its functions within the nucleus remain elusive. The role of this protein in replication of closed circular DNA containing a eukaryotic origin of replication has been studied in vitro by using native and recombinant HMGB1 as well as various modified HMGB1 preparations such as truncated protein, lacking its C-terminal tail, in vivo acetylated protein, and recombinant HMGB1 phosphorylated in vitro by protein kinase C (PKC). Native HMGB1 extracted from tumour cells inhibits replication and this effect is reduced upon acetylation and completely abolished upon removal of the acidic C-terminal tail. Recombinant HMGB1, however, fails to inhibit replication but it acquires such a property following in vitro phosphorylation by PKC.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>18191612</pmid><doi>10.1016/j.biocel.2007.11.014</doi><tpages>7</tpages></addata></record> |
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| ispartof | The international journal of biochemistry & cell biology, 2008, Vol.40 (8), p.1536-1542 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Acetylation Acidic tail Animals Aphidicolin - pharmacology DNA replication DNA Replication - drug effects DNA, Circular - metabolism High Mobility Group Proteins - pharmacology HMGB1 Protein Humans Phosphorylation Protein Kinase C - metabolism Rats Recombinant Proteins - pharmacology Repressor Proteins - pharmacology Tumor Cells, Cultured |
| title | HMGB1 protein inhibits DNA replication in vitro: A role of the acetylation and the acidic tail |
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