Parallel confocal detection of single molecules in real time
The confocal detection principle is extended to a highly parallel optical system that continuously analyzes thousands of concurrent sample locations. This is achieved through the use of a holographic laser illumination multiplexer combined with a confocal pinhole array before a prism dispersive elem...
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| Veröffentlicht in: | Optics letters 2008-05, Vol.33 (9), p.1026-1028 |
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| Hauptverfasser: | , , , , , , , , , , , , , , , , , |
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| container_end_page | 1028 |
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| container_issue | 9 |
| container_start_page | 1026 |
| container_title | Optics letters |
| container_volume | 33 |
| creator | Lundquist, Paul M Zhong, Cheng F Zhao, Peiqian Tomaney, Austin B Peluso, Paul S Dixon, John Bettman, Brad Lacroix, Yves Kwo, Deborah P McCullough, Etienne Maxham, Mark Hester, Kevin McNitt, Paul Grey, Donald M Henriquez, Carlos Foquet, Mathieu Turner, Stephen W Zaccarin, Denis |
| description | The confocal detection principle is extended to a highly parallel optical system that continuously analyzes thousands of concurrent sample locations. This is achieved through the use of a holographic laser illumination multiplexer combined with a confocal pinhole array before a prism dispersive element used to provide spectroscopic information from each confocal volume. The system is demonstrated to detect and identify single fluorescent molecules from each of several thousand independent confocal volumes in real time. |
| doi_str_mv | 10.1364/OL.33.001026 |
| format | Article |
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| identifier | ISSN: 0146-9592 |
| ispartof | Optics letters, 2008-05, Vol.33 (9), p.1026-1028 |
| issn | 0146-9592 |
| language | eng |
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| source | MEDLINE; Optica Publishing Group Journals |
| subjects | Fluorescent Dyes - chemistry Microscopy, Confocal - instrumentation Microscopy, Confocal - methods Nanotechnology |
| title | Parallel confocal detection of single molecules in real time |
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