Effect of follicular fluid collected from various diameter follicles on the progression of nuclear maturation and developmental competence of pig oocytes
Supplementing in vitro maturation medium with porcine follicular fluid (FF) improves maturation rate, male pronucleus formation, and monospermic fertilization of pig oocytes. This study examined, (1) if there are differences in FF derived from large follicles (LF, 5–6 mm in diameter) and small folli...
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| Veröffentlicht in: | Animal reproduction science 2008-07, Vol.106 (3), p.421-430 |
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| creator | Ito, M. Iwata, H. Kitagawa, M. Kon, Y. Kuwayama, T. Monji, Y. |
| description | Supplementing in vitro maturation medium with porcine follicular fluid (FF) improves maturation rate, male pronucleus formation, and monospermic fertilization of pig oocytes. This study examined, (1) if there are differences in FF derived from large follicles (LF, 5–6
mm in diameter) and small follicles (SF, 3–4
mm in diameter) on the effect of supplementing the maturation medium with FF on the progression of nuclear maturation, fertilization rate, and developmental competence of porcine oocytes; (2) whether the FF source influences the effect of the FF on the maturation medium on the survival rate and proliferation rate of cumulus cells (CCs) and the expansion of cumulus-oocyte-complexes (COCs); (3) whether the oocyte source (oocytes collected from LFs or SFs) influences the effect of FF on the progression of the nuclear maturation of oocytes; (4) whether the factors in the FF that affect the kinetics of nuclear maturation are proteins, and the range of the molecular weight of the FF factors.
In experiment 1, adding FF from LFs (LFF) significantly accelerated nuclear maturation and improved the fertilization rate; the developmental ratio was comparable with those of adding FF from SFs (SFF). In experiment 2, adding LFF, but not SFF, improved the CC survival rate, although the FF source did not affect the proliferation rate. Expansion of COCs was greater with SFF than LFF. In experiment 3, LFF promoted nuclear maturation of oocytes collected from only LFs. There was a significant interaction between the FF source and the oocyte source in the effect on nuclear maturation stages at 36
h of maturation. In experiment 4, treatment of FF with heat or trypsin diminished the difference between the effect of LFF and SFF on the progression of nuclear maturation. In addition, the predominant effect of LFF compared to that of SFF on nuclear maturation was not affected by ultrafiltration of the FF with a 30-kDa filter, but was diminished by ultrafiltration with a 100-kDa filter. The present study suggests that some proteins present in LFF that range in molecular weight from 30 to 100
kDa improve the developmental competence of oocytes probably via progression of nuclear maturation and cumulus cells viability. |
| doi_str_mv | 10.1016/j.anireprosci.2007.06.003 |
| format | Article |
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mm in diameter) and small follicles (SF, 3–4
mm in diameter) on the effect of supplementing the maturation medium with FF on the progression of nuclear maturation, fertilization rate, and developmental competence of porcine oocytes; (2) whether the FF source influences the effect of the FF on the maturation medium on the survival rate and proliferation rate of cumulus cells (CCs) and the expansion of cumulus-oocyte-complexes (COCs); (3) whether the oocyte source (oocytes collected from LFs or SFs) influences the effect of FF on the progression of the nuclear maturation of oocytes; (4) whether the factors in the FF that affect the kinetics of nuclear maturation are proteins, and the range of the molecular weight of the FF factors.
In experiment 1, adding FF from LFs (LFF) significantly accelerated nuclear maturation and improved the fertilization rate; the developmental ratio was comparable with those of adding FF from SFs (SFF). In experiment 2, adding LFF, but not SFF, improved the CC survival rate, although the FF source did not affect the proliferation rate. Expansion of COCs was greater with SFF than LFF. In experiment 3, LFF promoted nuclear maturation of oocytes collected from only LFs. There was a significant interaction between the FF source and the oocyte source in the effect on nuclear maturation stages at 36
h of maturation. In experiment 4, treatment of FF with heat or trypsin diminished the difference between the effect of LFF and SFF on the progression of nuclear maturation. In addition, the predominant effect of LFF compared to that of SFF on nuclear maturation was not affected by ultrafiltration of the FF with a 30-kDa filter, but was diminished by ultrafiltration with a 100-kDa filter. The present study suggests that some proteins present in LFF that range in molecular weight from 30 to 100
kDa improve the developmental competence of oocytes probably via progression of nuclear maturation and cumulus cells viability.</description><identifier>ISSN: 0378-4320</identifier><identifier>EISSN: 1873-2232</identifier><identifier>DOI: 10.1016/j.anireprosci.2007.06.003</identifier><identifier>PMID: 17644281</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>animal proteins ; Animals ; biochemical mechanisms ; blastocyst ; Cell Culture Techniques ; Cell Nucleus - drug effects ; Cell Nucleus - physiology ; cell proliferation ; Cell Proliferation - drug effects ; Cell Size - drug effects ; Cell Survival - drug effects ; Cells, Cultured ; culture media ; Culture Media - pharmacology ; cumulus oophorus ; diameter ; Female ; fertilization (reproduction) ; Fluid ; Follicular ; follicular development ; follicular fluid ; Follicular Fluid - physiology ; in vitro culture ; in vitro fertilization ; in vitro maturation ; Maturation ; Oocyte ; oocytes ; Oocytes - drug effects ; Oocytes - physiology ; Oogenesis - drug effects ; Oogenesis - physiology ; Ovarian Follicle - chemistry ; Ovarian Follicle - cytology ; Ovarian Follicle - physiology ; ovarian follicles ; Pig ; pronucleus ; protective effect ; swine ; Swine - physiology ; viability</subject><ispartof>Animal reproduction science, 2008-07, Vol.106 (3), p.421-430</ispartof><rights>2007 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c465t-c3262774373597713430228ffc033ed0bf038dccaf60d816db008af02c24e883</citedby><cites>FETCH-LOGICAL-c465t-c3262774373597713430228ffc033ed0bf038dccaf60d816db008af02c24e883</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378432007002060$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17644281$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ito, M.</creatorcontrib><creatorcontrib>Iwata, H.</creatorcontrib><creatorcontrib>Kitagawa, M.</creatorcontrib><creatorcontrib>Kon, Y.</creatorcontrib><creatorcontrib>Kuwayama, T.</creatorcontrib><creatorcontrib>Monji, Y.</creatorcontrib><title>Effect of follicular fluid collected from various diameter follicles on the progression of nuclear maturation and developmental competence of pig oocytes</title><title>Animal reproduction science</title><addtitle>Anim Reprod Sci</addtitle><description>Supplementing in vitro maturation medium with porcine follicular fluid (FF) improves maturation rate, male pronucleus formation, and monospermic fertilization of pig oocytes. This study examined, (1) if there are differences in FF derived from large follicles (LF, 5–6
mm in diameter) and small follicles (SF, 3–4
mm in diameter) on the effect of supplementing the maturation medium with FF on the progression of nuclear maturation, fertilization rate, and developmental competence of porcine oocytes; (2) whether the FF source influences the effect of the FF on the maturation medium on the survival rate and proliferation rate of cumulus cells (CCs) and the expansion of cumulus-oocyte-complexes (COCs); (3) whether the oocyte source (oocytes collected from LFs or SFs) influences the effect of FF on the progression of the nuclear maturation of oocytes; (4) whether the factors in the FF that affect the kinetics of nuclear maturation are proteins, and the range of the molecular weight of the FF factors.
In experiment 1, adding FF from LFs (LFF) significantly accelerated nuclear maturation and improved the fertilization rate; the developmental ratio was comparable with those of adding FF from SFs (SFF). In experiment 2, adding LFF, but not SFF, improved the CC survival rate, although the FF source did not affect the proliferation rate. Expansion of COCs was greater with SFF than LFF. In experiment 3, LFF promoted nuclear maturation of oocytes collected from only LFs. There was a significant interaction between the FF source and the oocyte source in the effect on nuclear maturation stages at 36
h of maturation. In experiment 4, treatment of FF with heat or trypsin diminished the difference between the effect of LFF and SFF on the progression of nuclear maturation. In addition, the predominant effect of LFF compared to that of SFF on nuclear maturation was not affected by ultrafiltration of the FF with a 30-kDa filter, but was diminished by ultrafiltration with a 100-kDa filter. The present study suggests that some proteins present in LFF that range in molecular weight from 30 to 100
kDa improve the developmental competence of oocytes probably via progression of nuclear maturation and cumulus cells viability.</description><subject>animal proteins</subject><subject>Animals</subject><subject>biochemical mechanisms</subject><subject>blastocyst</subject><subject>Cell Culture Techniques</subject><subject>Cell Nucleus - drug effects</subject><subject>Cell Nucleus - physiology</subject><subject>cell proliferation</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Size - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Cells, Cultured</subject><subject>culture media</subject><subject>Culture Media - pharmacology</subject><subject>cumulus oophorus</subject><subject>diameter</subject><subject>Female</subject><subject>fertilization (reproduction)</subject><subject>Fluid</subject><subject>Follicular</subject><subject>follicular development</subject><subject>follicular fluid</subject><subject>Follicular Fluid - physiology</subject><subject>in vitro culture</subject><subject>in vitro fertilization</subject><subject>in vitro maturation</subject><subject>Maturation</subject><subject>Oocyte</subject><subject>oocytes</subject><subject>Oocytes - drug effects</subject><subject>Oocytes - physiology</subject><subject>Oogenesis - drug effects</subject><subject>Oogenesis - physiology</subject><subject>Ovarian Follicle - chemistry</subject><subject>Ovarian Follicle - cytology</subject><subject>Ovarian Follicle - physiology</subject><subject>ovarian follicles</subject><subject>Pig</subject><subject>pronucleus</subject><subject>protective effect</subject><subject>swine</subject><subject>Swine - physiology</subject><subject>viability</subject><issn>0378-4320</issn><issn>1873-2232</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUcGO0zAUtBCI7RZ-AcyFW8qznTrpEVULi7QSB5az5drPxVUSBzuptJ-yf8uLWgmOnCz7zbwZzzD2QcBGgNCfThs7xIxjTsXFjQRoNqA3AOoFW4m2UZWUSr5kK1BNW9VKwg27LeUEBNR695rdiEbXtWzFij3fhYBu4inwkLouurmzmYdujp47eqAZeh5y6vnZ5pjmwn20PU6Yr4QOC08Dn34hJ0PHjKVEutPCYaYhbevtNGc7La928NzjGbs09jhMtiORfqRtg8OFMsYjT8k9TVjesFfBdgXfXs81e_xy97i_rx6-f_22__xQuVpvp8opqWXT1KpR213TCFUrkLINwYFS6OEQQLXeORs0-FZofwBobQDpZI1tq9bs42Utmf89Y5lMH4vDrrMD0m-N3olGbCnQNdtdgI5iLxmDGXPsbX4yAsxSizmZf2oxSy0GtKFaiPvuKjIfevR_mdceCPD-Agg2GXvMsZifPyQIRWZ3W6EWn_sLAimLc8RsSGSJzZOkm4xP8T-M_AHeH7Gh</recordid><startdate>20080701</startdate><enddate>20080701</enddate><creator>Ito, M.</creator><creator>Iwata, H.</creator><creator>Kitagawa, M.</creator><creator>Kon, Y.</creator><creator>Kuwayama, T.</creator><creator>Monji, Y.</creator><general>Elsevier B.V</general><general>[Amsterdam]: Elsevier Science</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080701</creationdate><title>Effect of follicular fluid collected from various diameter follicles on the progression of nuclear maturation and developmental competence of pig oocytes</title><author>Ito, M. ; Iwata, H. ; Kitagawa, M. ; Kon, Y. ; Kuwayama, T. ; Monji, Y.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c465t-c3262774373597713430228ffc033ed0bf038dccaf60d816db008af02c24e883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>animal proteins</topic><topic>Animals</topic><topic>biochemical mechanisms</topic><topic>blastocyst</topic><topic>Cell Culture Techniques</topic><topic>Cell Nucleus - drug effects</topic><topic>Cell Nucleus - physiology</topic><topic>cell proliferation</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Size - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>Cells, Cultured</topic><topic>culture media</topic><topic>Culture Media - pharmacology</topic><topic>cumulus oophorus</topic><topic>diameter</topic><topic>Female</topic><topic>fertilization (reproduction)</topic><topic>Fluid</topic><topic>Follicular</topic><topic>follicular development</topic><topic>follicular fluid</topic><topic>Follicular Fluid - physiology</topic><topic>in vitro culture</topic><topic>in vitro fertilization</topic><topic>in vitro maturation</topic><topic>Maturation</topic><topic>Oocyte</topic><topic>oocytes</topic><topic>Oocytes - drug effects</topic><topic>Oocytes - physiology</topic><topic>Oogenesis - drug effects</topic><topic>Oogenesis - physiology</topic><topic>Ovarian Follicle - chemistry</topic><topic>Ovarian Follicle - cytology</topic><topic>Ovarian Follicle - physiology</topic><topic>ovarian follicles</topic><topic>Pig</topic><topic>pronucleus</topic><topic>protective effect</topic><topic>swine</topic><topic>Swine - physiology</topic><topic>viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ito, M.</creatorcontrib><creatorcontrib>Iwata, H.</creatorcontrib><creatorcontrib>Kitagawa, M.</creatorcontrib><creatorcontrib>Kon, Y.</creatorcontrib><creatorcontrib>Kuwayama, T.</creatorcontrib><creatorcontrib>Monji, Y.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Animal reproduction science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ito, M.</au><au>Iwata, H.</au><au>Kitagawa, M.</au><au>Kon, Y.</au><au>Kuwayama, T.</au><au>Monji, Y.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of follicular fluid collected from various diameter follicles on the progression of nuclear maturation and developmental competence of pig oocytes</atitle><jtitle>Animal reproduction science</jtitle><addtitle>Anim Reprod Sci</addtitle><date>2008-07-01</date><risdate>2008</risdate><volume>106</volume><issue>3</issue><spage>421</spage><epage>430</epage><pages>421-430</pages><issn>0378-4320</issn><eissn>1873-2232</eissn><abstract>Supplementing in vitro maturation medium with porcine follicular fluid (FF) improves maturation rate, male pronucleus formation, and monospermic fertilization of pig oocytes. This study examined, (1) if there are differences in FF derived from large follicles (LF, 5–6
mm in diameter) and small follicles (SF, 3–4
mm in diameter) on the effect of supplementing the maturation medium with FF on the progression of nuclear maturation, fertilization rate, and developmental competence of porcine oocytes; (2) whether the FF source influences the effect of the FF on the maturation medium on the survival rate and proliferation rate of cumulus cells (CCs) and the expansion of cumulus-oocyte-complexes (COCs); (3) whether the oocyte source (oocytes collected from LFs or SFs) influences the effect of FF on the progression of the nuclear maturation of oocytes; (4) whether the factors in the FF that affect the kinetics of nuclear maturation are proteins, and the range of the molecular weight of the FF factors.
In experiment 1, adding FF from LFs (LFF) significantly accelerated nuclear maturation and improved the fertilization rate; the developmental ratio was comparable with those of adding FF from SFs (SFF). In experiment 2, adding LFF, but not SFF, improved the CC survival rate, although the FF source did not affect the proliferation rate. Expansion of COCs was greater with SFF than LFF. In experiment 3, LFF promoted nuclear maturation of oocytes collected from only LFs. There was a significant interaction between the FF source and the oocyte source in the effect on nuclear maturation stages at 36
h of maturation. In experiment 4, treatment of FF with heat or trypsin diminished the difference between the effect of LFF and SFF on the progression of nuclear maturation. In addition, the predominant effect of LFF compared to that of SFF on nuclear maturation was not affected by ultrafiltration of the FF with a 30-kDa filter, but was diminished by ultrafiltration with a 100-kDa filter. The present study suggests that some proteins present in LFF that range in molecular weight from 30 to 100
kDa improve the developmental competence of oocytes probably via progression of nuclear maturation and cumulus cells viability.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>17644281</pmid><doi>10.1016/j.anireprosci.2007.06.003</doi><tpages>10</tpages></addata></record> |
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| ispartof | Animal reproduction science, 2008-07, Vol.106 (3), p.421-430 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | animal proteins Animals biochemical mechanisms blastocyst Cell Culture Techniques Cell Nucleus - drug effects Cell Nucleus - physiology cell proliferation Cell Proliferation - drug effects Cell Size - drug effects Cell Survival - drug effects Cells, Cultured culture media Culture Media - pharmacology cumulus oophorus diameter Female fertilization (reproduction) Fluid Follicular follicular development follicular fluid Follicular Fluid - physiology in vitro culture in vitro fertilization in vitro maturation Maturation Oocyte oocytes Oocytes - drug effects Oocytes - physiology Oogenesis - drug effects Oogenesis - physiology Ovarian Follicle - chemistry Ovarian Follicle - cytology Ovarian Follicle - physiology ovarian follicles Pig pronucleus protective effect swine Swine - physiology viability |
| title | Effect of follicular fluid collected from various diameter follicles on the progression of nuclear maturation and developmental competence of pig oocytes |
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