Epitope specificities and antibody responses to the EG95 hydatid vaccine
Antibody isotype and epitope specificities were examined in sheep immunized with EG95, a protective recombinant vaccine against hydatid disease. All sheep immunized with EG95 as a fusion protein with glutathione S‐transferase (GST) produced prominent IgG antibodies against the EG95 portion of the pr...
Gespeichert in:
| Veröffentlicht in: | Parasite immunology 1998-11, Vol.20 (11), p.535-540 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 540 |
|---|---|
| container_issue | 11 |
| container_start_page | 535 |
| container_title | Parasite immunology |
| container_volume | 20 |
| creator | Woollard, D.J. Gauci, C.G. Heath, D.D. Lightowlers, M.W. |
| description | Antibody isotype and epitope specificities were examined in sheep immunized with EG95, a protective recombinant vaccine against hydatid disease. All sheep immunized with EG95 as a fusion protein with glutathione S‐transferase (GST) produced prominent IgG antibodies against the EG95 portion of the protein. Linear, antibody‐binding epitope specificities of EG95 were mapped using a series of 25 overlapping synthetic peptides. Three immunodominant regions were identified which generated specific IgG1 and IgG2 antibodies in the majority of vaccinated sheep. These regions corresponded to the EG95‐derived sequences SLKAVNPSDPLVYKRQTAKF, DIETPRAGKKESTVMTSGSA and SALTSAIAGFVFSC. An additional immunogenic region was identified which induced almost exclusively IgG2 antibody. This epitope was located within the sequence TETPLRKHFNLTPV. The anti‐parasitic, protective effects of the EG95 vaccine correlated with the detection of specific antibody to two or more of the four linear immunogenic regions. The identification of these immunogenic peptides of EG95 maybe useful in the development of a synthetic peptide vaccine as a derivative of the EG95 recombinant. |
| doi_str_mv | 10.1046/j.1365-3024.1998.00176.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69169347</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>69169347</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3946-40afe93dfe59d6fbe1136e9e7b5962c4f0c66f3d2d6c7b739885452a74b68df13</originalsourceid><addsrcrecordid>eNqNkM1OwzAQhC0EKqXwCEg5cUuw45_EEhdUlbZSERzgbCX2WnWVNiFOoXl7HFpxhcPKK8_M7upDKCI4IZiJ-01CqOAxxSlLiJR5gjHJRHI4Q-Nf4RyNMWEkljnNL9GV95tgoqmgIzQKkZwSPEaLWeO6uoHIN6Cdddp1DnxU7EyozpW16aMWfFPvfPju6qhbQzSbSx6te1N0zkSfhdZuB9fowhaVh5vTO0HvT7O36SJevcyX08dVrKlkIma4sCCpscClEbYEEu4FCVnJpUg1s1gLYalJjdBZmdFwJ2c8LTJWitxYQifo7ji3aeuPPfhObZ3XUFXFDuq9V0ISISnL_jSSjDDOuQjG_GjUbe19C1Y1rdsWba8IVgNttVEDVDVAVQNt9UNbHUL09rRjX27B_AZPeIP-cNS_XAX9v-eq1-VzaOg3r1GNMA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17145556</pqid></control><display><type>article</type><title>Epitope specificities and antibody responses to the EG95 hydatid vaccine</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Access via Wiley Online Library</source><source>IngentaConnect Free/Open Access Journals</source><source>Wiley Online Library (Open Access Collection)</source><source>Alma/SFX Local Collection</source><creator>Woollard, D.J. ; Gauci, C.G. ; Heath, D.D. ; Lightowlers, M.W.</creator><creatorcontrib>Woollard, D.J. ; Gauci, C.G. ; Heath, D.D. ; Lightowlers, M.W.</creatorcontrib><description>Antibody isotype and epitope specificities were examined in sheep immunized with EG95, a protective recombinant vaccine against hydatid disease. All sheep immunized with EG95 as a fusion protein with glutathione S‐transferase (GST) produced prominent IgG antibodies against the EG95 portion of the protein. Linear, antibody‐binding epitope specificities of EG95 were mapped using a series of 25 overlapping synthetic peptides. Three immunodominant regions were identified which generated specific IgG1 and IgG2 antibodies in the majority of vaccinated sheep. These regions corresponded to the EG95‐derived sequences SLKAVNPSDPLVYKRQTAKF, DIETPRAGKKESTVMTSGSA and SALTSAIAGFVFSC. An additional immunogenic region was identified which induced almost exclusively IgG2 antibody. This epitope was located within the sequence TETPLRKHFNLTPV. The anti‐parasitic, protective effects of the EG95 vaccine correlated with the detection of specific antibody to two or more of the four linear immunogenic regions. The identification of these immunogenic peptides of EG95 maybe useful in the development of a synthetic peptide vaccine as a derivative of the EG95 recombinant.</description><identifier>ISSN: 0141-9838</identifier><identifier>EISSN: 1365-3024</identifier><identifier>DOI: 10.1046/j.1365-3024.1998.00176.x</identifier><identifier>PMID: 9988310</identifier><language>eng</language><publisher>Oxford, U.K. and Cambridge, USA: Blackwell Science Ltd</publisher><subject>Amino Acid Sequence ; Animals ; Antibodies, Helminth - blood ; Antibodies, Helminth - immunology ; antibody ; Antibody Specificity - immunology ; Antigens, Helminth - immunology ; Antigens, Helminth - metabolism ; Echinococcosis - prevention & control ; Echinococcosis - veterinary ; Echinococcus - immunology ; Echinococcus granulosus ; epitope ; Epitope Mapping ; hydatid ; Immunodominant Epitopes - immunology ; Immunoglobulin Isotypes ; Molecular Sequence Data ; Peptides - chemistry ; Peptides - immunology ; Recombinant Fusion Proteins ; Sheep ; Sheep Diseases - immunology ; Vaccination ; vaccine ; Vaccines, Synthetic - administration & dosage ; Vaccines, Synthetic - immunology</subject><ispartof>Parasite immunology, 1998-11, Vol.20 (11), p.535-540</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3946-40afe93dfe59d6fbe1136e9e7b5962c4f0c66f3d2d6c7b739885452a74b68df13</citedby><cites>FETCH-LOGICAL-c3946-40afe93dfe59d6fbe1136e9e7b5962c4f0c66f3d2d6c7b739885452a74b68df13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-3024.1998.00176.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-3024.1998.00176.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,1434,27929,27930,45579,45580,46414,46838</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9988310$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Woollard, D.J.</creatorcontrib><creatorcontrib>Gauci, C.G.</creatorcontrib><creatorcontrib>Heath, D.D.</creatorcontrib><creatorcontrib>Lightowlers, M.W.</creatorcontrib><title>Epitope specificities and antibody responses to the EG95 hydatid vaccine</title><title>Parasite immunology</title><addtitle>Parasite Immunol</addtitle><description>Antibody isotype and epitope specificities were examined in sheep immunized with EG95, a protective recombinant vaccine against hydatid disease. All sheep immunized with EG95 as a fusion protein with glutathione S‐transferase (GST) produced prominent IgG antibodies against the EG95 portion of the protein. Linear, antibody‐binding epitope specificities of EG95 were mapped using a series of 25 overlapping synthetic peptides. Three immunodominant regions were identified which generated specific IgG1 and IgG2 antibodies in the majority of vaccinated sheep. These regions corresponded to the EG95‐derived sequences SLKAVNPSDPLVYKRQTAKF, DIETPRAGKKESTVMTSGSA and SALTSAIAGFVFSC. An additional immunogenic region was identified which induced almost exclusively IgG2 antibody. This epitope was located within the sequence TETPLRKHFNLTPV. The anti‐parasitic, protective effects of the EG95 vaccine correlated with the detection of specific antibody to two or more of the four linear immunogenic regions. The identification of these immunogenic peptides of EG95 maybe useful in the development of a synthetic peptide vaccine as a derivative of the EG95 recombinant.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies, Helminth - blood</subject><subject>Antibodies, Helminth - immunology</subject><subject>antibody</subject><subject>Antibody Specificity - immunology</subject><subject>Antigens, Helminth - immunology</subject><subject>Antigens, Helminth - metabolism</subject><subject>Echinococcosis - prevention & control</subject><subject>Echinococcosis - veterinary</subject><subject>Echinococcus - immunology</subject><subject>Echinococcus granulosus</subject><subject>epitope</subject><subject>Epitope Mapping</subject><subject>hydatid</subject><subject>Immunodominant Epitopes - immunology</subject><subject>Immunoglobulin Isotypes</subject><subject>Molecular Sequence Data</subject><subject>Peptides - chemistry</subject><subject>Peptides - immunology</subject><subject>Recombinant Fusion Proteins</subject><subject>Sheep</subject><subject>Sheep Diseases - immunology</subject><subject>Vaccination</subject><subject>vaccine</subject><subject>Vaccines, Synthetic - administration & dosage</subject><subject>Vaccines, Synthetic - immunology</subject><issn>0141-9838</issn><issn>1365-3024</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkM1OwzAQhC0EKqXwCEg5cUuw45_EEhdUlbZSERzgbCX2WnWVNiFOoXl7HFpxhcPKK8_M7upDKCI4IZiJ-01CqOAxxSlLiJR5gjHJRHI4Q-Nf4RyNMWEkljnNL9GV95tgoqmgIzQKkZwSPEaLWeO6uoHIN6Cdddp1DnxU7EyozpW16aMWfFPvfPju6qhbQzSbSx6te1N0zkSfhdZuB9fowhaVh5vTO0HvT7O36SJevcyX08dVrKlkIma4sCCpscClEbYEEu4FCVnJpUg1s1gLYalJjdBZmdFwJ2c8LTJWitxYQifo7ji3aeuPPfhObZ3XUFXFDuq9V0ISISnL_jSSjDDOuQjG_GjUbe19C1Y1rdsWba8IVgNttVEDVDVAVQNt9UNbHUL09rRjX27B_AZPeIP-cNS_XAX9v-eq1-VzaOg3r1GNMA</recordid><startdate>199811</startdate><enddate>199811</enddate><creator>Woollard, D.J.</creator><creator>Gauci, C.G.</creator><creator>Heath, D.D.</creator><creator>Lightowlers, M.W.</creator><general>Blackwell Science Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>199811</creationdate><title>Epitope specificities and antibody responses to the EG95 hydatid vaccine</title><author>Woollard, D.J. ; Gauci, C.G. ; Heath, D.D. ; Lightowlers, M.W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3946-40afe93dfe59d6fbe1136e9e7b5962c4f0c66f3d2d6c7b739885452a74b68df13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibodies, Helminth - blood</topic><topic>Antibodies, Helminth - immunology</topic><topic>antibody</topic><topic>Antibody Specificity - immunology</topic><topic>Antigens, Helminth - immunology</topic><topic>Antigens, Helminth - metabolism</topic><topic>Echinococcosis - prevention & control</topic><topic>Echinococcosis - veterinary</topic><topic>Echinococcus - immunology</topic><topic>Echinococcus granulosus</topic><topic>epitope</topic><topic>Epitope Mapping</topic><topic>hydatid</topic><topic>Immunodominant Epitopes - immunology</topic><topic>Immunoglobulin Isotypes</topic><topic>Molecular Sequence Data</topic><topic>Peptides - chemistry</topic><topic>Peptides - immunology</topic><topic>Recombinant Fusion Proteins</topic><topic>Sheep</topic><topic>Sheep Diseases - immunology</topic><topic>Vaccination</topic><topic>vaccine</topic><topic>Vaccines, Synthetic - administration & dosage</topic><topic>Vaccines, Synthetic - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Woollard, D.J.</creatorcontrib><creatorcontrib>Gauci, C.G.</creatorcontrib><creatorcontrib>Heath, D.D.</creatorcontrib><creatorcontrib>Lightowlers, M.W.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Parasite immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Woollard, D.J.</au><au>Gauci, C.G.</au><au>Heath, D.D.</au><au>Lightowlers, M.W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Epitope specificities and antibody responses to the EG95 hydatid vaccine</atitle><jtitle>Parasite immunology</jtitle><addtitle>Parasite Immunol</addtitle><date>1998-11</date><risdate>1998</risdate><volume>20</volume><issue>11</issue><spage>535</spage><epage>540</epage><pages>535-540</pages><issn>0141-9838</issn><eissn>1365-3024</eissn><abstract>Antibody isotype and epitope specificities were examined in sheep immunized with EG95, a protective recombinant vaccine against hydatid disease. All sheep immunized with EG95 as a fusion protein with glutathione S‐transferase (GST) produced prominent IgG antibodies against the EG95 portion of the protein. Linear, antibody‐binding epitope specificities of EG95 were mapped using a series of 25 overlapping synthetic peptides. Three immunodominant regions were identified which generated specific IgG1 and IgG2 antibodies in the majority of vaccinated sheep. These regions corresponded to the EG95‐derived sequences SLKAVNPSDPLVYKRQTAKF, DIETPRAGKKESTVMTSGSA and SALTSAIAGFVFSC. An additional immunogenic region was identified which induced almost exclusively IgG2 antibody. This epitope was located within the sequence TETPLRKHFNLTPV. The anti‐parasitic, protective effects of the EG95 vaccine correlated with the detection of specific antibody to two or more of the four linear immunogenic regions. The identification of these immunogenic peptides of EG95 maybe useful in the development of a synthetic peptide vaccine as a derivative of the EG95 recombinant.</abstract><cop>Oxford, U.K. and Cambridge, USA</cop><pub>Blackwell Science Ltd</pub><pmid>9988310</pmid><doi>10.1046/j.1365-3024.1998.00176.x</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0141-9838 |
| ispartof | Parasite immunology, 1998-11, Vol.20 (11), p.535-540 |
| issn | 0141-9838 1365-3024 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69169347 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Access via Wiley Online Library; IngentaConnect Free/Open Access Journals; Wiley Online Library (Open Access Collection); Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Animals Antibodies, Helminth - blood Antibodies, Helminth - immunology antibody Antibody Specificity - immunology Antigens, Helminth - immunology Antigens, Helminth - metabolism Echinococcosis - prevention & control Echinococcosis - veterinary Echinococcus - immunology Echinococcus granulosus epitope Epitope Mapping hydatid Immunodominant Epitopes - immunology Immunoglobulin Isotypes Molecular Sequence Data Peptides - chemistry Peptides - immunology Recombinant Fusion Proteins Sheep Sheep Diseases - immunology Vaccination vaccine Vaccines, Synthetic - administration & dosage Vaccines, Synthetic - immunology |
| title | Epitope specificities and antibody responses to the EG95 hydatid vaccine |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-12T15%3A58%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Epitope%20specificities%20and%20antibody%20responses%20to%20the%20EG95%20hydatid%20vaccine&rft.jtitle=Parasite%20immunology&rft.au=Woollard,%20D.J.&rft.date=1998-11&rft.volume=20&rft.issue=11&rft.spage=535&rft.epage=540&rft.pages=535-540&rft.issn=0141-9838&rft.eissn=1365-3024&rft_id=info:doi/10.1046/j.1365-3024.1998.00176.x&rft_dat=%3Cproquest_cross%3E69169347%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17145556&rft_id=info:pmid/9988310&rfr_iscdi=true |