A chicken homolog of mammalian interleukin‐1β : cDNA cloning and purification of active recombinant protein

Upon induction with lipopolysaccharide (LPS) the chicken macrophage cell line HD‐11 secretes an activity that stimulates the synthesis of a CXC chemokine in the chicken fibroblast cell line CEC‐32. We used a cDNA expression cloning strategy in COS cells to characterize this activity. The isolated cD...

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Veröffentlicht in:	European journal of biochemistry 1998-12, Vol.258 (3), p.994-1000
Hauptverfasser:	Weining, Kirsten C., Sick, Christine, Kaspers, Bernd, Staeheli, Peter
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Base Sequence cDNA expression cloning Cell Line Cell-Free System Chemokines, CXC - biosynthesis Chick Embryo chicken Chickens Cloning, Molecular COS Cells CXC chemokine DNA, Complementary - genetics DNA, Complementary - isolation & purification Escherichia coli Histidine - metabolism Humans Interleukin-1 - genetics Interleukin-1 - isolation & purification Interleukin-1 - physiology interleukin‐1β lipopolysaccharide inducibility Lipopolysaccharides - pharmacology Macrophages - drug effects Macrophages - metabolism Molecular Sequence Data Recombinant Proteins - chemistry Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Sequence Homology, Nucleic Acid Transcription, Genetic
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