Molecular cloning of hSLP-1, a novel human brain-specific member of the band 7/MEC-2 family similar to Caenorhabditis elegans UNC-24
We have isolated and characterized cDNA clones encoding a stomatin-like protein (hSLP-1) from a human cerebral cortex cDNA library. The deduced amino acid sequence (394 residues) revealed that hSLP-1 is a bipartite protein, containing a major stomatin-like part, starting at the N-terminus, and a non...
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| Veröffentlicht in: | Gene 1998-12, Vol.225 (1), p.23-29 |
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| description | We have isolated and characterized cDNA clones encoding a stomatin-like protein (hSLP-1) from a human cerebral cortex cDNA library. The deduced amino acid sequence (394 residues) revealed that hSLP-1 is a bipartite protein, containing a major stomatin-like part, starting at the N-terminus, and a non-specific lipid transfer protein (nsLTP)-domain at the C-terminal end, similar to the
Caenorhabditis elegans protein UNC-24. Therefore, we conclude that hSLP-1 is the human homologue of UNC-24. In addition, the identification of an alternatively spliced variant demonstrated that two exon/intron boundaries are conserved in the h
SLP-1 and
unc-24 genes. Northern blot and RNA dot blot analyses showed that the 2.2-kb transcript is mainly expressed in the brain, with the highest levels in the frontal lobe, cerebral cortex, caudate nucleus, amygdala, temporal lobe, putamen, substantia nigra, and hippocampus. This high-level expression of hSLP-1 in the basal ganglia may also reflect the evolutionary link to UNC-24. |
| doi_str_mv | 10.1016/S0378-1119(98)00532-0 |
| format | Article |
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Caenorhabditis elegans protein UNC-24. Therefore, we conclude that hSLP-1 is the human homologue of UNC-24. In addition, the identification of an alternatively spliced variant demonstrated that two exon/intron boundaries are conserved in the h
SLP-1 and
unc-24 genes. Northern blot and RNA dot blot analyses showed that the 2.2-kb transcript is mainly expressed in the brain, with the highest levels in the frontal lobe, cerebral cortex, caudate nucleus, amygdala, temporal lobe, putamen, substantia nigra, and hippocampus. This high-level expression of hSLP-1 in the basal ganglia may also reflect the evolutionary link to UNC-24.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/S0378-1119(98)00532-0</identifier><identifier>PMID: 9931417</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Alternative splicing ; Amino Acid Sequence ; Animals ; Base Sequence ; Blood Proteins - genetics ; Blotting, Northern ; Brain - metabolism ; Caenorhabditis elegans - genetics ; Caenorhabditis elegans Proteins ; Cloning, Molecular ; DNA, Complementary - chemistry ; DNA, Complementary - genetics ; DNA, Complementary - isolation & purification ; Gene Expression ; Helminth Proteins - genetics ; Humans ; Immunoblotting ; Lipid transfer protein ; Membrane protein ; Membrane Proteins - genetics ; Molecular Sequence Data ; Nerve Tissue Proteins ; Nervous system ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Sequence Alignment ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; Stomatin ; Tissue Distribution</subject><ispartof>Gene, 1998-12, Vol.225 (1), p.23-29</ispartof><rights>1998 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c360t-45fc99f2dbc7dc608610e61744bf7caebf00efb204e1fae081824f40a1ab8f713</citedby><cites>FETCH-LOGICAL-c360t-45fc99f2dbc7dc608610e61744bf7caebf00efb204e1fae081824f40a1ab8f713</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0378-1119(98)00532-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9931417$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Seidel, Günther</creatorcontrib><creatorcontrib>Prohaska, Rainer</creatorcontrib><title>Molecular cloning of hSLP-1, a novel human brain-specific member of the band 7/MEC-2 family similar to Caenorhabditis elegans UNC-24</title><title>Gene</title><addtitle>Gene</addtitle><description>We have isolated and characterized cDNA clones encoding a stomatin-like protein (hSLP-1) from a human cerebral cortex cDNA library. The deduced amino acid sequence (394 residues) revealed that hSLP-1 is a bipartite protein, containing a major stomatin-like part, starting at the N-terminus, and a non-specific lipid transfer protein (nsLTP)-domain at the C-terminal end, similar to the
Caenorhabditis elegans protein UNC-24. Therefore, we conclude that hSLP-1 is the human homologue of UNC-24. In addition, the identification of an alternatively spliced variant demonstrated that two exon/intron boundaries are conserved in the h
SLP-1 and
unc-24 genes. Northern blot and RNA dot blot analyses showed that the 2.2-kb transcript is mainly expressed in the brain, with the highest levels in the frontal lobe, cerebral cortex, caudate nucleus, amygdala, temporal lobe, putamen, substantia nigra, and hippocampus. This high-level expression of hSLP-1 in the basal ganglia may also reflect the evolutionary link to UNC-24.</description><subject>Alternative splicing</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Blood Proteins - genetics</subject><subject>Blotting, Northern</subject><subject>Brain - metabolism</subject><subject>Caenorhabditis elegans - genetics</subject><subject>Caenorhabditis elegans Proteins</subject><subject>Cloning, Molecular</subject><subject>DNA, Complementary - chemistry</subject><subject>DNA, Complementary - genetics</subject><subject>DNA, Complementary - isolation & purification</subject><subject>Gene Expression</subject><subject>Helminth Proteins - genetics</subject><subject>Humans</subject><subject>Immunoblotting</subject><subject>Lipid transfer protein</subject><subject>Membrane protein</subject><subject>Membrane Proteins - genetics</subject><subject>Molecular Sequence Data</subject><subject>Nerve Tissue Proteins</subject><subject>Nervous system</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><subject>Stomatin</subject><subject>Tissue Distribution</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFu1DAQhi0EKkvhESr5hEDCdJw4iX1C1aq0SFtAKj1btjPuGiX2YieVeufByXZXvTKX_zDfzGg-Qs44fObA2_NbqDvJOOfqg5IfAZq6YvCCrLjsFAOo5UuyekZekzel_IalmqY6ISdK1VzwbkX-3qQB3TyYTN2QYoj3NHm6vd38ZPwTNTSmBxzodh5NpDabEFnZoQs-ODriaDHv8WmL1JrY0-785nLNKurNGIZHWsISy-Yp0bXBmPLW2D5MoVAc8N7EQu--L7h4S155MxR8d8xTcvf18tf6mm1-XH1bX2yYq1uYmGi8U8pXvXVd71qQLQdseSeE9Z0zaD0AeluBQO4NguSyEl6A4cZK3_H6lLw_7N3l9GfGMukxFIfDYCKmuehW8VZA3SxgcwBdTqVk9HqXw2jyo-ag9_b1k329V6uV1E_2NSxzZ8cDsx2xf5466l76Xw59XL58CJh1cQGjwz5kdJPuU_jPhX8eWpOJ</recordid><startdate>19981228</startdate><enddate>19981228</enddate><creator>Seidel, Günther</creator><creator>Prohaska, Rainer</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19981228</creationdate><title>Molecular cloning of hSLP-1, a novel human brain-specific member of the band 7/MEC-2 family similar to Caenorhabditis elegans UNC-24</title><author>Seidel, Günther ; Prohaska, Rainer</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-45fc99f2dbc7dc608610e61744bf7caebf00efb204e1fae081824f40a1ab8f713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Alternative splicing</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Blood Proteins - genetics</topic><topic>Blotting, Northern</topic><topic>Brain - metabolism</topic><topic>Caenorhabditis elegans - genetics</topic><topic>Caenorhabditis elegans Proteins</topic><topic>Cloning, Molecular</topic><topic>DNA, Complementary - chemistry</topic><topic>DNA, Complementary - genetics</topic><topic>DNA, Complementary - isolation & purification</topic><topic>Gene Expression</topic><topic>Helminth Proteins - genetics</topic><topic>Humans</topic><topic>Immunoblotting</topic><topic>Lipid transfer protein</topic><topic>Membrane protein</topic><topic>Membrane Proteins - genetics</topic><topic>Molecular Sequence Data</topic><topic>Nerve Tissue Proteins</topic><topic>Nervous system</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Sequence Alignment</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><topic>Stomatin</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Seidel, Günther</creatorcontrib><creatorcontrib>Prohaska, Rainer</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Seidel, Günther</au><au>Prohaska, Rainer</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning of hSLP-1, a novel human brain-specific member of the band 7/MEC-2 family similar to Caenorhabditis elegans UNC-24</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1998-12-28</date><risdate>1998</risdate><volume>225</volume><issue>1</issue><spage>23</spage><epage>29</epage><pages>23-29</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>We have isolated and characterized cDNA clones encoding a stomatin-like protein (hSLP-1) from a human cerebral cortex cDNA library. The deduced amino acid sequence (394 residues) revealed that hSLP-1 is a bipartite protein, containing a major stomatin-like part, starting at the N-terminus, and a non-specific lipid transfer protein (nsLTP)-domain at the C-terminal end, similar to the
Caenorhabditis elegans protein UNC-24. Therefore, we conclude that hSLP-1 is the human homologue of UNC-24. In addition, the identification of an alternatively spliced variant demonstrated that two exon/intron boundaries are conserved in the h
SLP-1 and
unc-24 genes. Northern blot and RNA dot blot analyses showed that the 2.2-kb transcript is mainly expressed in the brain, with the highest levels in the frontal lobe, cerebral cortex, caudate nucleus, amygdala, temporal lobe, putamen, substantia nigra, and hippocampus. This high-level expression of hSLP-1 in the basal ganglia may also reflect the evolutionary link to UNC-24.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>9931417</pmid><doi>10.1016/S0378-1119(98)00532-0</doi><tpages>7</tpages></addata></record> |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Alternative splicing Amino Acid Sequence Animals Base Sequence Blood Proteins - genetics Blotting, Northern Brain - metabolism Caenorhabditis elegans - genetics Caenorhabditis elegans Proteins Cloning, Molecular DNA, Complementary - chemistry DNA, Complementary - genetics DNA, Complementary - isolation & purification Gene Expression Helminth Proteins - genetics Humans Immunoblotting Lipid transfer protein Membrane protein Membrane Proteins - genetics Molecular Sequence Data Nerve Tissue Proteins Nervous system RNA, Messenger - genetics RNA, Messenger - metabolism Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid Stomatin Tissue Distribution |
| title | Molecular cloning of hSLP-1, a novel human brain-specific member of the band 7/MEC-2 family similar to Caenorhabditis elegans UNC-24 |
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