Heat treatment mitigates calcification of valvular bioprostheses
Background. Several techniques have been proposed to mitigate calcification of glutaraldehyde-preserved bioprostheses. None has been fully satisfactory. Knowing that heat induces significant molecular changes, we investigated the potential benefit of the heat treatment of glutaraldehyde-fixed tissue...
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| Veröffentlicht in: | The Annals of thoracic surgery 1998-12, Vol.66 (6), p.S264-S266 |
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| container_title | The Annals of thoracic surgery |
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| creator | Carpentier, Sophie M Chen, Lin Shen, Ming Fornes, Paul Martinet, Bernard Quintero, Lillian J Witzel, Thomas H Carpentier, Alain F |
| description | Background. Several techniques have been proposed to mitigate calcification of glutaraldehyde-preserved bioprostheses. None has been fully satisfactory. Knowing that heat induces significant molecular changes, we investigated the potential benefit of the heat treatment of glutaraldehyde-fixed tissues.
Methods. Samples of porcine valves and bovine pericardium treated in 0.625% glutaraldehyde were submitted to temperatures from 37° to 70°C for 2 to 12 weeks and then implanted subcutaneously in newborn rats for 3 months. In a second protocol, nine heat-treated porcine valve bioprostheses and seven control porcine valve bioprostheses were implanted in the mitral position in sheep for 20 weeks.
Results. Spectrophotometry and x-ray analysis comparing heat-treated versus non–heat-treated tissues showed the following: porcine valve: 6.7 ± 2.3 μg Ca/mg (n = 8) versus 239.9 ± 2.9 μg Ca/mg (n = 8); bovine pericardium: 19.5 ± 8 μg Ca/mg (n = 6) versus 108 ± 10.3 μg Ca/mg (n = 8); and porcine valve bioprostheses: 0 versus ++.
Conclusions. Heat treatment of glutaraldehyde-treated bioprostheses significantly mitigated calcification in both subcutaneous and mitral position. |
| doi_str_mv | 10.1016/S0003-4975(98)01101-1 |
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Methods. Samples of porcine valves and bovine pericardium treated in 0.625% glutaraldehyde were submitted to temperatures from 37° to 70°C for 2 to 12 weeks and then implanted subcutaneously in newborn rats for 3 months. In a second protocol, nine heat-treated porcine valve bioprostheses and seven control porcine valve bioprostheses were implanted in the mitral position in sheep for 20 weeks.
Results. Spectrophotometry and x-ray analysis comparing heat-treated versus non–heat-treated tissues showed the following: porcine valve: 6.7 ± 2.3 μg Ca/mg (n = 8) versus 239.9 ± 2.9 μg Ca/mg (n = 8); bovine pericardium: 19.5 ± 8 μg Ca/mg (n = 6) versus 108 ± 10.3 μg Ca/mg (n = 8); and porcine valve bioprostheses: 0 versus ++.
Conclusions. Heat treatment of glutaraldehyde-treated bioprostheses significantly mitigated calcification in both subcutaneous and mitral position.</description><identifier>ISSN: 0003-4975</identifier><identifier>EISSN: 1552-6259</identifier><identifier>DOI: 10.1016/S0003-4975(98)01101-1</identifier><identifier>PMID: 9930461</identifier><identifier>CODEN: ATHSAK</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Animals ; Animals, Newborn ; Aortic Valve ; Biological and medical sciences ; bioprostheses ; Bioprosthesis ; calcification ; Calcinosis - prevention & control ; Calcium - analysis ; Cardiology. Vascular system ; Cattle ; Endocardial and cardiac valvular diseases ; Female ; Fixatives - chemistry ; Glutaral - chemistry ; glutaraldehyde ; Heart ; Heart Valve Prosthesis ; Heart Valve Prosthesis Implantation ; Hot Temperature ; Male ; Medical sciences ; Mitral Valve - surgery ; pericardium ; porcine valves ; Prosthesis Design ; Rats ; Rats, Wistar ; Sheep ; Spectrophotometry ; Swine ; Time Factors ; X-Rays</subject><ispartof>The Annals of thoracic surgery, 1998-12, Vol.66 (6), p.S264-S266</ispartof><rights>1998 The Society of Thoracic Surgeons</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c455t-1a9a14a668a5921d4eaaf1c699eac8929242eea8383ba27a531b139205dff7b13</citedby><cites>FETCH-LOGICAL-c455t-1a9a14a668a5921d4eaaf1c699eac8929242eea8383ba27a531b139205dff7b13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0003497598011011$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,776,780,785,786,3536,23910,23911,25119,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1685861$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9930461$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Carpentier, Sophie M</creatorcontrib><creatorcontrib>Chen, Lin</creatorcontrib><creatorcontrib>Shen, Ming</creatorcontrib><creatorcontrib>Fornes, Paul</creatorcontrib><creatorcontrib>Martinet, Bernard</creatorcontrib><creatorcontrib>Quintero, Lillian J</creatorcontrib><creatorcontrib>Witzel, Thomas H</creatorcontrib><creatorcontrib>Carpentier, Alain F</creatorcontrib><title>Heat treatment mitigates calcification of valvular bioprostheses</title><title>The Annals of thoracic surgery</title><addtitle>Ann Thorac Surg</addtitle><description>Background. Several techniques have been proposed to mitigate calcification of glutaraldehyde-preserved bioprostheses. None has been fully satisfactory. Knowing that heat induces significant molecular changes, we investigated the potential benefit of the heat treatment of glutaraldehyde-fixed tissues.
Methods. Samples of porcine valves and bovine pericardium treated in 0.625% glutaraldehyde were submitted to temperatures from 37° to 70°C for 2 to 12 weeks and then implanted subcutaneously in newborn rats for 3 months. In a second protocol, nine heat-treated porcine valve bioprostheses and seven control porcine valve bioprostheses were implanted in the mitral position in sheep for 20 weeks.
Results. Spectrophotometry and x-ray analysis comparing heat-treated versus non–heat-treated tissues showed the following: porcine valve: 6.7 ± 2.3 μg Ca/mg (n = 8) versus 239.9 ± 2.9 μg Ca/mg (n = 8); bovine pericardium: 19.5 ± 8 μg Ca/mg (n = 6) versus 108 ± 10.3 μg Ca/mg (n = 8); and porcine valve bioprostheses: 0 versus ++.
Conclusions. Heat treatment of glutaraldehyde-treated bioprostheses significantly mitigated calcification in both subcutaneous and mitral position.</description><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Aortic Valve</subject><subject>Biological and medical sciences</subject><subject>bioprostheses</subject><subject>Bioprosthesis</subject><subject>calcification</subject><subject>Calcinosis - prevention & control</subject><subject>Calcium - analysis</subject><subject>Cardiology. Vascular system</subject><subject>Cattle</subject><subject>Endocardial and cardiac valvular diseases</subject><subject>Female</subject><subject>Fixatives - chemistry</subject><subject>Glutaral - chemistry</subject><subject>glutaraldehyde</subject><subject>Heart</subject><subject>Heart Valve Prosthesis</subject><subject>Heart Valve Prosthesis Implantation</subject><subject>Hot Temperature</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mitral Valve - surgery</subject><subject>pericardium</subject><subject>porcine valves</subject><subject>Prosthesis Design</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Sheep</subject><subject>Spectrophotometry</subject><subject>Swine</subject><subject>Time Factors</subject><subject>X-Rays</subject><issn>0003-4975</issn><issn>1552-6259</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkElLBDEQhYMo47j8BKEPInpoTaU7mc5JRdxgwIN6DjXpao30oklmwH9vZkGPXiqVvJd6xcfYEfBz4KAunjnnRV7qiTzV1RmH9JjDFhuDlCJXQuptNv617LK9ED7SVSR5xEZaF7xUMGZXD4Qxiz7VjvqYdS66N4wUMoutdY2zGN3QZ0OTLbBdzFv02cwNn34I8Z0ChQO202Ab6HBz7rPXu9uXm4d8-nT_eHM9zW0pZcwBNUKJSlUotYC6JMQGrNKa0FZaaFEKIqyKqpihmKAsYAaFFlzWTTNJ7T47Wc9N0V9zCtF0LlhqW-xpmAejNCgAoZNRro027Rg8NebTuw79twFuluTMipxZYjG6MityZhlwtAmYzzqqf39tUCX9eKNjSGwaj7114W-4qmS1sl2ubZRgLBx5E6yj3lLtPNlo6sH9s8gPb4KKRA</recordid><startdate>19981201</startdate><enddate>19981201</enddate><creator>Carpentier, Sophie M</creator><creator>Chen, Lin</creator><creator>Shen, Ming</creator><creator>Fornes, Paul</creator><creator>Martinet, Bernard</creator><creator>Quintero, Lillian J</creator><creator>Witzel, Thomas H</creator><creator>Carpentier, Alain F</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19981201</creationdate><title>Heat treatment mitigates calcification of valvular bioprostheses</title><author>Carpentier, Sophie M ; Chen, Lin ; Shen, Ming ; Fornes, Paul ; Martinet, Bernard ; Quintero, Lillian J ; Witzel, Thomas H ; Carpentier, Alain F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c455t-1a9a14a668a5921d4eaaf1c699eac8929242eea8383ba27a531b139205dff7b13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Aortic Valve</topic><topic>Biological and medical sciences</topic><topic>bioprostheses</topic><topic>Bioprosthesis</topic><topic>calcification</topic><topic>Calcinosis - prevention & control</topic><topic>Calcium - analysis</topic><topic>Cardiology. Vascular system</topic><topic>Cattle</topic><topic>Endocardial and cardiac valvular diseases</topic><topic>Female</topic><topic>Fixatives - chemistry</topic><topic>Glutaral - chemistry</topic><topic>glutaraldehyde</topic><topic>Heart</topic><topic>Heart Valve Prosthesis</topic><topic>Heart Valve Prosthesis Implantation</topic><topic>Hot Temperature</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mitral Valve - surgery</topic><topic>pericardium</topic><topic>porcine valves</topic><topic>Prosthesis Design</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Sheep</topic><topic>Spectrophotometry</topic><topic>Swine</topic><topic>Time Factors</topic><topic>X-Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Carpentier, Sophie M</creatorcontrib><creatorcontrib>Chen, Lin</creatorcontrib><creatorcontrib>Shen, Ming</creatorcontrib><creatorcontrib>Fornes, Paul</creatorcontrib><creatorcontrib>Martinet, Bernard</creatorcontrib><creatorcontrib>Quintero, Lillian J</creatorcontrib><creatorcontrib>Witzel, Thomas H</creatorcontrib><creatorcontrib>Carpentier, Alain F</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Annals of thoracic surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Carpentier, Sophie M</au><au>Chen, Lin</au><au>Shen, Ming</au><au>Fornes, Paul</au><au>Martinet, Bernard</au><au>Quintero, Lillian J</au><au>Witzel, Thomas H</au><au>Carpentier, Alain F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Heat treatment mitigates calcification of valvular bioprostheses</atitle><jtitle>The Annals of thoracic surgery</jtitle><addtitle>Ann Thorac Surg</addtitle><date>1998-12-01</date><risdate>1998</risdate><volume>66</volume><issue>6</issue><spage>S264</spage><epage>S266</epage><pages>S264-S266</pages><issn>0003-4975</issn><eissn>1552-6259</eissn><coden>ATHSAK</coden><abstract>Background. Several techniques have been proposed to mitigate calcification of glutaraldehyde-preserved bioprostheses. None has been fully satisfactory. Knowing that heat induces significant molecular changes, we investigated the potential benefit of the heat treatment of glutaraldehyde-fixed tissues.
Methods. Samples of porcine valves and bovine pericardium treated in 0.625% glutaraldehyde were submitted to temperatures from 37° to 70°C for 2 to 12 weeks and then implanted subcutaneously in newborn rats for 3 months. In a second protocol, nine heat-treated porcine valve bioprostheses and seven control porcine valve bioprostheses were implanted in the mitral position in sheep for 20 weeks.
Results. Spectrophotometry and x-ray analysis comparing heat-treated versus non–heat-treated tissues showed the following: porcine valve: 6.7 ± 2.3 μg Ca/mg (n = 8) versus 239.9 ± 2.9 μg Ca/mg (n = 8); bovine pericardium: 19.5 ± 8 μg Ca/mg (n = 6) versus 108 ± 10.3 μg Ca/mg (n = 8); and porcine valve bioprostheses: 0 versus ++.
Conclusions. Heat treatment of glutaraldehyde-treated bioprostheses significantly mitigated calcification in both subcutaneous and mitral position.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>9930461</pmid><doi>10.1016/S0003-4975(98)01101-1</doi></addata></record> |
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| subjects | Animals Animals, Newborn Aortic Valve Biological and medical sciences bioprostheses Bioprosthesis calcification Calcinosis - prevention & control Calcium - analysis Cardiology. Vascular system Cattle Endocardial and cardiac valvular diseases Female Fixatives - chemistry Glutaral - chemistry glutaraldehyde Heart Heart Valve Prosthesis Heart Valve Prosthesis Implantation Hot Temperature Male Medical sciences Mitral Valve - surgery pericardium porcine valves Prosthesis Design Rats Rats, Wistar Sheep Spectrophotometry Swine Time Factors X-Rays |
| title | Heat treatment mitigates calcification of valvular bioprostheses |
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