Substance P regulates the expression of matrix metalloproteinases and tissue inhibitors of metalloproteinase in cultured human gingival fibroblasts
Background and Objective: Substance P may play a role in the pathogenesis of periodontal disease; however, its mechanisms of modulation are not clear. This study evaluated the effect of two concentrations of Substance P on the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of...
Gespeichert in:
| Veröffentlicht in: | Journal of periodontal research 2008-06, Vol.43 (3), p.255-260 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 260 |
|---|---|
| container_issue | 3 |
| container_start_page | 255 |
| container_title | Journal of periodontal research |
| container_volume | 43 |
| creator | Cury, P. R. Canavez, F. De Araújo, V. C. Furuse, C. De Araújo, N. S. |
| description | Background and Objective: Substance P may play a role in the pathogenesis of periodontal disease; however, its mechanisms of modulation are not clear. This study evaluated the effect of two concentrations of Substance P on the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in cultured human gingival fibroblasts.
Material and Methods: Fibroblasts were stimulated for 48 h with 10−4 or 10−9 m Substance P; untreated fibroblasts served as controls. The expression of MMP‐1, ‐2, ‐3, ‐7 and ‐11 and of TIMP‐1 and ‐2 was evaluated using real‐time polymerase chain reaction and western blotting.
Results: There was a significant, concentration‐dependent stimulatory effect of Substance P on MMP‐1, ‐2, ‐3 and ‐7 and TIMP‐2 gene expression (p |
| doi_str_mv | 10.1111/j.1600-0765.2007.01022.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69159693</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>69159693</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4352-22d3d2317ecdc9de15c1b0e86aded85749cb29e4a2dba55473d425560f45c6c03</originalsourceid><addsrcrecordid>eNqNkc9u1DAQxiMEokvhFZAvcEuwnTh_DhxQKVuqChAFcbQce7LrxUkWjwPpc_DCON3VInHCF9szv88zni9JCKMZi-vVLmMlpSmtSpFxSquMMsp5Nj9IVqfEw2RFYzDNi7o4S54g7mi8l1XzODljNauaospXye_bqcWgBg3kE_GwmZwKgCRsgcC894Box4GMHelV8HYmPQTl3Lj3YwA7KIysGgwJFnECYoetbW0YPd5L_mVjnujJhcmDIdupVwPZ2GFjfypHOtv6sXUKAz5NHnXKITw77ufJ13eXXy6u0puP6_cXb25SXeSCp5yb3PCcVaCNbgwwoVlLoS6VAVOLqmh0yxsoFDetEiJ-1xRciJJ2hdClpvl58vLwbuzwxwQYZG9Rg3NqgHFCWTZMNGWTR7A-gNqPiB46ufe2V_5OMioXQ-ROLnOXy9zlYoi8N0TOUfr8WGNqezB_hUcHIvDiCCjUynU-emHxxHGaM1oUPHKvD9wv6-DuvxuQ158vl1PUpwe9xQDzSa_8d1lWeSXktw9reXt91azfikry_A_GX7n7</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69159693</pqid></control><display><type>article</type><title>Substance P regulates the expression of matrix metalloproteinases and tissue inhibitors of metalloproteinase in cultured human gingival fibroblasts</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Cury, P. R. ; Canavez, F. ; De Araújo, V. C. ; Furuse, C. ; De Araújo, N. S.</creator><creatorcontrib>Cury, P. R. ; Canavez, F. ; De Araújo, V. C. ; Furuse, C. ; De Araújo, N. S.</creatorcontrib><description>Background and Objective: Substance P may play a role in the pathogenesis of periodontal disease; however, its mechanisms of modulation are not clear. This study evaluated the effect of two concentrations of Substance P on the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in cultured human gingival fibroblasts.
Material and Methods: Fibroblasts were stimulated for 48 h with 10−4 or 10−9 m Substance P; untreated fibroblasts served as controls. The expression of MMP‐1, ‐2, ‐3, ‐7 and ‐11 and of TIMP‐1 and ‐2 was evaluated using real‐time polymerase chain reaction and western blotting.
Results: There was a significant, concentration‐dependent stimulatory effect of Substance P on MMP‐1, ‐2, ‐3 and ‐7 and TIMP‐2 gene expression (p < 0.05), and a probable effect on MMP‐11 (p = 0.06). At the higher concentration (10−4 m Substance P), MMP‐1, ‐2, ‐3, ‐7 and ‐11 and TIMP‐2 showed the greatest up‐regulation; at the lower concentration (10−9 m Substance P), MMP‐1, ‐3 and ‐7 and TIMP‐2 exhibited diminished up‐regulation, with MMP‐2 and ‐11 showing down‐regulation (p < 0.05). Expression of TIMP‐1 was not affected by Substance P (p > 0.05). Western blotting confirmed that Substance P up‐regulated MMP‐1, ‐2, ‐3 and ‐11 and TIMP‐2. MMP‐1, ‐3 and ‐11 and TIMP‐2 showed greater up‐regulation at the higher Substance P concentration and diminished up‐regulation at the lower concentration. MMP‐2 was up‐regulated to a similar degree at both Substance P concentrations.
Conclusion: In gingival fibroblast cells, Substance P at the higher concentration (10−4 m) induced greater up‐regulation of MMP‐1, ‐3 and ‐11 and TIMP‐2 expression, but at the lower concentration (10−9 m) induced diminished up‐regulation, which may represent a mechanism for modulating periodontal breakdown.</description><identifier>ISSN: 0022-3484</identifier><identifier>EISSN: 1600-0765</identifier><identifier>DOI: 10.1111/j.1600-0765.2007.01022.x</identifier><identifier>PMID: 18179473</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Biological and medical sciences ; Blotting, Western ; Cells, Cultured ; Dentistry ; Dose-Response Relationship, Drug ; Enzyme Activation ; Fibroblasts - enzymology ; Gene Expression ; Gingiva - cytology ; Gingiva - enzymology ; gingival fibroblasts ; Humans ; matrix metalloproteinase ; Matrix Metalloproteinases - biosynthesis ; Medical sciences ; Otorhinolaryngology. Stomatology ; Polymerase Chain Reaction ; Statistics, Nonparametric ; Substance P ; Substance P - administration & dosage ; Substance P - pharmacology ; Substance P - physiology ; tissue inhibitor of metalloproteinase ; Tissue Inhibitor of Metalloproteinases - biosynthesis ; Up-Regulation</subject><ispartof>Journal of periodontal research, 2008-06, Vol.43 (3), p.255-260</ispartof><rights>2007 The Authors. Journal compilation © 2007 Blackwell Munksgaard</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4352-22d3d2317ecdc9de15c1b0e86aded85749cb29e4a2dba55473d425560f45c6c03</citedby><cites>FETCH-LOGICAL-c4352-22d3d2317ecdc9de15c1b0e86aded85749cb29e4a2dba55473d425560f45c6c03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1600-0765.2007.01022.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1600-0765.2007.01022.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20310442$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18179473$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cury, P. R.</creatorcontrib><creatorcontrib>Canavez, F.</creatorcontrib><creatorcontrib>De Araújo, V. C.</creatorcontrib><creatorcontrib>Furuse, C.</creatorcontrib><creatorcontrib>De Araújo, N. S.</creatorcontrib><title>Substance P regulates the expression of matrix metalloproteinases and tissue inhibitors of metalloproteinase in cultured human gingival fibroblasts</title><title>Journal of periodontal research</title><addtitle>J Periodontal Res</addtitle><description>Background and Objective: Substance P may play a role in the pathogenesis of periodontal disease; however, its mechanisms of modulation are not clear. This study evaluated the effect of two concentrations of Substance P on the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in cultured human gingival fibroblasts.
Material and Methods: Fibroblasts were stimulated for 48 h with 10−4 or 10−9 m Substance P; untreated fibroblasts served as controls. The expression of MMP‐1, ‐2, ‐3, ‐7 and ‐11 and of TIMP‐1 and ‐2 was evaluated using real‐time polymerase chain reaction and western blotting.
Results: There was a significant, concentration‐dependent stimulatory effect of Substance P on MMP‐1, ‐2, ‐3 and ‐7 and TIMP‐2 gene expression (p < 0.05), and a probable effect on MMP‐11 (p = 0.06). At the higher concentration (10−4 m Substance P), MMP‐1, ‐2, ‐3, ‐7 and ‐11 and TIMP‐2 showed the greatest up‐regulation; at the lower concentration (10−9 m Substance P), MMP‐1, ‐3 and ‐7 and TIMP‐2 exhibited diminished up‐regulation, with MMP‐2 and ‐11 showing down‐regulation (p < 0.05). Expression of TIMP‐1 was not affected by Substance P (p > 0.05). Western blotting confirmed that Substance P up‐regulated MMP‐1, ‐2, ‐3 and ‐11 and TIMP‐2. MMP‐1, ‐3 and ‐11 and TIMP‐2 showed greater up‐regulation at the higher Substance P concentration and diminished up‐regulation at the lower concentration. MMP‐2 was up‐regulated to a similar degree at both Substance P concentrations.
Conclusion: In gingival fibroblast cells, Substance P at the higher concentration (10−4 m) induced greater up‐regulation of MMP‐1, ‐3 and ‐11 and TIMP‐2 expression, but at the lower concentration (10−9 m) induced diminished up‐regulation, which may represent a mechanism for modulating periodontal breakdown.</description><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Cells, Cultured</subject><subject>Dentistry</subject><subject>Dose-Response Relationship, Drug</subject><subject>Enzyme Activation</subject><subject>Fibroblasts - enzymology</subject><subject>Gene Expression</subject><subject>Gingiva - cytology</subject><subject>Gingiva - enzymology</subject><subject>gingival fibroblasts</subject><subject>Humans</subject><subject>matrix metalloproteinase</subject><subject>Matrix Metalloproteinases - biosynthesis</subject><subject>Medical sciences</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>Polymerase Chain Reaction</subject><subject>Statistics, Nonparametric</subject><subject>Substance P</subject><subject>Substance P - administration & dosage</subject><subject>Substance P - pharmacology</subject><subject>Substance P - physiology</subject><subject>tissue inhibitor of metalloproteinase</subject><subject>Tissue Inhibitor of Metalloproteinases - biosynthesis</subject><subject>Up-Regulation</subject><issn>0022-3484</issn><issn>1600-0765</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc9u1DAQxiMEokvhFZAvcEuwnTh_DhxQKVuqChAFcbQce7LrxUkWjwPpc_DCON3VInHCF9szv88zni9JCKMZi-vVLmMlpSmtSpFxSquMMsp5Nj9IVqfEw2RFYzDNi7o4S54g7mi8l1XzODljNauaospXye_bqcWgBg3kE_GwmZwKgCRsgcC894Box4GMHelV8HYmPQTl3Lj3YwA7KIysGgwJFnECYoetbW0YPd5L_mVjnujJhcmDIdupVwPZ2GFjfypHOtv6sXUKAz5NHnXKITw77ufJ13eXXy6u0puP6_cXb25SXeSCp5yb3PCcVaCNbgwwoVlLoS6VAVOLqmh0yxsoFDetEiJ-1xRciJJ2hdClpvl58vLwbuzwxwQYZG9Rg3NqgHFCWTZMNGWTR7A-gNqPiB46ufe2V_5OMioXQ-ROLnOXy9zlYoi8N0TOUfr8WGNqezB_hUcHIvDiCCjUynU-emHxxHGaM1oUPHKvD9wv6-DuvxuQ158vl1PUpwe9xQDzSa_8d1lWeSXktw9reXt91azfikry_A_GX7n7</recordid><startdate>200806</startdate><enddate>200806</enddate><creator>Cury, P. R.</creator><creator>Canavez, F.</creator><creator>De Araújo, V. C.</creator><creator>Furuse, C.</creator><creator>De Araújo, N. S.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200806</creationdate><title>Substance P regulates the expression of matrix metalloproteinases and tissue inhibitors of metalloproteinase in cultured human gingival fibroblasts</title><author>Cury, P. R. ; Canavez, F. ; De Araújo, V. C. ; Furuse, C. ; De Araújo, N. S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4352-22d3d2317ecdc9de15c1b0e86aded85749cb29e4a2dba55473d425560f45c6c03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Cells, Cultured</topic><topic>Dentistry</topic><topic>Dose-Response Relationship, Drug</topic><topic>Enzyme Activation</topic><topic>Fibroblasts - enzymology</topic><topic>Gene Expression</topic><topic>Gingiva - cytology</topic><topic>Gingiva - enzymology</topic><topic>gingival fibroblasts</topic><topic>Humans</topic><topic>matrix metalloproteinase</topic><topic>Matrix Metalloproteinases - biosynthesis</topic><topic>Medical sciences</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>Polymerase Chain Reaction</topic><topic>Statistics, Nonparametric</topic><topic>Substance P</topic><topic>Substance P - administration & dosage</topic><topic>Substance P - pharmacology</topic><topic>Substance P - physiology</topic><topic>tissue inhibitor of metalloproteinase</topic><topic>Tissue Inhibitor of Metalloproteinases - biosynthesis</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cury, P. R.</creatorcontrib><creatorcontrib>Canavez, F.</creatorcontrib><creatorcontrib>De Araújo, V. C.</creatorcontrib><creatorcontrib>Furuse, C.</creatorcontrib><creatorcontrib>De Araújo, N. S.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of periodontal research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cury, P. R.</au><au>Canavez, F.</au><au>De Araújo, V. C.</au><au>Furuse, C.</au><au>De Araújo, N. S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Substance P regulates the expression of matrix metalloproteinases and tissue inhibitors of metalloproteinase in cultured human gingival fibroblasts</atitle><jtitle>Journal of periodontal research</jtitle><addtitle>J Periodontal Res</addtitle><date>2008-06</date><risdate>2008</risdate><volume>43</volume><issue>3</issue><spage>255</spage><epage>260</epage><pages>255-260</pages><issn>0022-3484</issn><eissn>1600-0765</eissn><abstract>Background and Objective: Substance P may play a role in the pathogenesis of periodontal disease; however, its mechanisms of modulation are not clear. This study evaluated the effect of two concentrations of Substance P on the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in cultured human gingival fibroblasts.
Material and Methods: Fibroblasts were stimulated for 48 h with 10−4 or 10−9 m Substance P; untreated fibroblasts served as controls. The expression of MMP‐1, ‐2, ‐3, ‐7 and ‐11 and of TIMP‐1 and ‐2 was evaluated using real‐time polymerase chain reaction and western blotting.
Results: There was a significant, concentration‐dependent stimulatory effect of Substance P on MMP‐1, ‐2, ‐3 and ‐7 and TIMP‐2 gene expression (p < 0.05), and a probable effect on MMP‐11 (p = 0.06). At the higher concentration (10−4 m Substance P), MMP‐1, ‐2, ‐3, ‐7 and ‐11 and TIMP‐2 showed the greatest up‐regulation; at the lower concentration (10−9 m Substance P), MMP‐1, ‐3 and ‐7 and TIMP‐2 exhibited diminished up‐regulation, with MMP‐2 and ‐11 showing down‐regulation (p < 0.05). Expression of TIMP‐1 was not affected by Substance P (p > 0.05). Western blotting confirmed that Substance P up‐regulated MMP‐1, ‐2, ‐3 and ‐11 and TIMP‐2. MMP‐1, ‐3 and ‐11 and TIMP‐2 showed greater up‐regulation at the higher Substance P concentration and diminished up‐regulation at the lower concentration. MMP‐2 was up‐regulated to a similar degree at both Substance P concentrations.
Conclusion: In gingival fibroblast cells, Substance P at the higher concentration (10−4 m) induced greater up‐regulation of MMP‐1, ‐3 and ‐11 and TIMP‐2 expression, but at the lower concentration (10−9 m) induced diminished up‐regulation, which may represent a mechanism for modulating periodontal breakdown.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>18179473</pmid><doi>10.1111/j.1600-0765.2007.01022.x</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-3484 |
| ispartof | Journal of periodontal research, 2008-06, Vol.43 (3), p.255-260 |
| issn | 0022-3484 1600-0765 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69159693 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Biological and medical sciences Blotting, Western Cells, Cultured Dentistry Dose-Response Relationship, Drug Enzyme Activation Fibroblasts - enzymology Gene Expression Gingiva - cytology Gingiva - enzymology gingival fibroblasts Humans matrix metalloproteinase Matrix Metalloproteinases - biosynthesis Medical sciences Otorhinolaryngology. Stomatology Polymerase Chain Reaction Statistics, Nonparametric Substance P Substance P - administration & dosage Substance P - pharmacology Substance P - physiology tissue inhibitor of metalloproteinase Tissue Inhibitor of Metalloproteinases - biosynthesis Up-Regulation |
| title | Substance P regulates the expression of matrix metalloproteinases and tissue inhibitors of metalloproteinase in cultured human gingival fibroblasts |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-25T14%3A39%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Substance%20P%20regulates%20the%20expression%20of%20matrix%20metalloproteinases%20and%20tissue%20inhibitors%20of%20metalloproteinase%20in%20cultured%20human%20gingival%20fibroblasts&rft.jtitle=Journal%20of%20periodontal%20research&rft.au=Cury,%20P.%20R.&rft.date=2008-06&rft.volume=43&rft.issue=3&rft.spage=255&rft.epage=260&rft.pages=255-260&rft.issn=0022-3484&rft.eissn=1600-0765&rft_id=info:doi/10.1111/j.1600-0765.2007.01022.x&rft_dat=%3Cproquest_cross%3E69159693%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=69159693&rft_id=info:pmid/18179473&rfr_iscdi=true |