Differential sensitivity to nickel and SK&F96365 of second messenger-operated and receptor-operated calcium channels in rat submandibular ductal cells
The intracellular concentration of calcium ([Ca2+]i) of rat submandibular ductal cells was measured with the intracellular fluorescent dye Fura-2. Carbachol (100 microM) and ATP (1 mM) both increased the [Ca2+]i. The late response to ATP was blocked by 0.5 mM Ni2+. This concentration of Ni2+ also bl...
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| Veröffentlicht in: | Cell calcium (Edinburgh) 1998-06, Vol.23 (6), p.395-404 |
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| creator | Chaïb, N Kabré, E Métioui, M Alzola, E Dantinne, C Marino, A Dehaye, J P |
| description | The intracellular concentration of calcium ([Ca2+]i) of rat submandibular ductal cells was measured with the intracellular fluorescent dye Fura-2. Carbachol (100 microM) and ATP (1 mM) both increased the [Ca2+]i. The late response to ATP was blocked by 0.5 mM Ni2+. This concentration of Ni2+ also blocked the increase of the [Ca2+]i and the uptake of manganese and calcium in response to 2'- and 3'-O-(4-benzoylbenzoyl) adenosine 5'-triphosphate (BzATP, 100 microM), a specific agonist of P2X receptors from salivary glands. The increase of the [Ca2+]i in response to 2-methylthioadenosine 5'-triphosphate (2-MeSATP, 100 microM) a specific P2Y agonist in salivary glands or to a muscarinic agonist (carbachol) was not affected by 0.5 mM Ni2+. Only higher concentrations of Ni2+ (in the millimolar range) inhibited the uptake of extracellular calcium in response to carbachol. SK&F96365, a blocker of store-operated calcium channels, inhibited the uptake of extracellular calcium in response to carbachol without affecting the response to BzATP. It is concluded that at low concentrations (below 0.5 mM), Ni2+ inhibits the non-specific cation channel coupled to P2X receptors. The uptake of extracellular calcium by store-operated calcium channels is inhibited by higher concentrations of Ni2+ and by SK&F96365. |
| format | Article |
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Carbachol (100 microM) and ATP (1 mM) both increased the [Ca2+]i. The late response to ATP was blocked by 0.5 mM Ni2+. This concentration of Ni2+ also blocked the increase of the [Ca2+]i and the uptake of manganese and calcium in response to 2'- and 3'-O-(4-benzoylbenzoyl) adenosine 5'-triphosphate (BzATP, 100 microM), a specific agonist of P2X receptors from salivary glands. The increase of the [Ca2+]i in response to 2-methylthioadenosine 5'-triphosphate (2-MeSATP, 100 microM) a specific P2Y agonist in salivary glands or to a muscarinic agonist (carbachol) was not affected by 0.5 mM Ni2+. Only higher concentrations of Ni2+ (in the millimolar range) inhibited the uptake of extracellular calcium in response to carbachol. SK&F96365, a blocker of store-operated calcium channels, inhibited the uptake of extracellular calcium in response to carbachol without affecting the response to BzATP. It is concluded that at low concentrations (below 0.5 mM), Ni2+ inhibits the non-specific cation channel coupled to P2X receptors. The uptake of extracellular calcium by store-operated calcium channels is inhibited by higher concentrations of Ni2+ and by SK&F96365.</description><identifier>ISSN: 0143-4160</identifier><identifier>PMID: 9924631</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Adenosine Triphosphate - analogs & derivatives ; Adenosine Triphosphate - pharmacology ; Affinity Labels ; Animals ; Calcium - metabolism ; Calcium Channel Blockers - metabolism ; Calcium Channels - metabolism ; Calcium Chloride - pharmacology ; Carbachol - pharmacology ; Dose-Response Relationship, Drug ; Imidazoles - pharmacology ; Male ; Manganese - metabolism ; Muscarinic Agonists - pharmacology ; Nickel - metabolism ; Nickel - pharmacology ; Rats ; Rats, Wistar ; Receptors, Purinergic - metabolism ; Second Messenger Systems - physiology ; Submandibular Gland - metabolism ; Thionucleotides - pharmacology ; Time Factors</subject><ispartof>Cell calcium (Edinburgh), 1998-06, Vol.23 (6), p.395-404</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9924631$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chaïb, N</creatorcontrib><creatorcontrib>Kabré, E</creatorcontrib><creatorcontrib>Métioui, M</creatorcontrib><creatorcontrib>Alzola, E</creatorcontrib><creatorcontrib>Dantinne, C</creatorcontrib><creatorcontrib>Marino, A</creatorcontrib><creatorcontrib>Dehaye, J P</creatorcontrib><title>Differential sensitivity to nickel and SK&F96365 of second messenger-operated and receptor-operated calcium channels in rat submandibular ductal cells</title><title>Cell calcium (Edinburgh)</title><addtitle>Cell Calcium</addtitle><description>The intracellular concentration of calcium ([Ca2+]i) of rat submandibular ductal cells was measured with the intracellular fluorescent dye Fura-2. Carbachol (100 microM) and ATP (1 mM) both increased the [Ca2+]i. The late response to ATP was blocked by 0.5 mM Ni2+. This concentration of Ni2+ also blocked the increase of the [Ca2+]i and the uptake of manganese and calcium in response to 2'- and 3'-O-(4-benzoylbenzoyl) adenosine 5'-triphosphate (BzATP, 100 microM), a specific agonist of P2X receptors from salivary glands. The increase of the [Ca2+]i in response to 2-methylthioadenosine 5'-triphosphate (2-MeSATP, 100 microM) a specific P2Y agonist in salivary glands or to a muscarinic agonist (carbachol) was not affected by 0.5 mM Ni2+. Only higher concentrations of Ni2+ (in the millimolar range) inhibited the uptake of extracellular calcium in response to carbachol. SK&F96365, a blocker of store-operated calcium channels, inhibited the uptake of extracellular calcium in response to carbachol without affecting the response to BzATP. It is concluded that at low concentrations (below 0.5 mM), Ni2+ inhibits the non-specific cation channel coupled to P2X receptors. The uptake of extracellular calcium by store-operated calcium channels is inhibited by higher concentrations of Ni2+ and by SK&F96365.</description><subject>Adenosine Triphosphate - analogs & derivatives</subject><subject>Adenosine Triphosphate - pharmacology</subject><subject>Affinity Labels</subject><subject>Animals</subject><subject>Calcium - metabolism</subject><subject>Calcium Channel Blockers - metabolism</subject><subject>Calcium Channels - metabolism</subject><subject>Calcium Chloride - pharmacology</subject><subject>Carbachol - pharmacology</subject><subject>Dose-Response Relationship, Drug</subject><subject>Imidazoles - pharmacology</subject><subject>Male</subject><subject>Manganese - metabolism</subject><subject>Muscarinic Agonists - pharmacology</subject><subject>Nickel - metabolism</subject><subject>Nickel - pharmacology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Receptors, Purinergic - metabolism</subject><subject>Second Messenger Systems - physiology</subject><subject>Submandibular Gland - metabolism</subject><subject>Thionucleotides - pharmacology</subject><subject>Time Factors</subject><issn>0143-4160</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkDFPwzAQhTOASin8BCRP3SLFduzUIyoUEJUY6B459hkMjhNsB6l_hN-LCx3YmE56973Te3dSzCtc07LGvDorzmN8q6pK0AbPipkQpOYUz4uvG2sMBPDJSoci-GiT_bRpj9KAvFXv4JD0Gj0_LjeCU87QYDKmhqz1ELPhBUI5jBBkAv2DBlAwpuGPqqRTduqRepXeg4vIepQ3KE5dnx22m5wMSE8q5QwKnIsXxamRLsLlcS6K3eZ2t74vt093D-vrbTmualzyTkupiQQhOOuoJrQhjTaGA6G8ER0hNQOjmaolXUkGqtGiYxIDUYZ0NdBFsfw9O4bhY4KY2t7GQwDpYZhiywVmnIrmXxBzVokGH8CrI5i7gW7HYHsZ9u3x4fQbneN-xA</recordid><startdate>199806</startdate><enddate>199806</enddate><creator>Chaïb, N</creator><creator>Kabré, E</creator><creator>Métioui, M</creator><creator>Alzola, E</creator><creator>Dantinne, C</creator><creator>Marino, A</creator><creator>Dehaye, J P</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QP</scope><scope>7X8</scope></search><sort><creationdate>199806</creationdate><title>Differential sensitivity to nickel and SK&F96365 of second messenger-operated and receptor-operated calcium channels in rat submandibular ductal cells</title><author>Chaïb, N ; Kabré, E ; Métioui, M ; Alzola, E ; Dantinne, C ; Marino, A ; Dehaye, J P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p841-6bdaad2ae9965b3d23727dff6e23679b2245efd5c4a38a5ec7d9b5a1e2cf2b4e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Adenosine Triphosphate - analogs & derivatives</topic><topic>Adenosine Triphosphate - pharmacology</topic><topic>Affinity Labels</topic><topic>Animals</topic><topic>Calcium - metabolism</topic><topic>Calcium Channel Blockers - metabolism</topic><topic>Calcium Channels - metabolism</topic><topic>Calcium Chloride - pharmacology</topic><topic>Carbachol - pharmacology</topic><topic>Dose-Response Relationship, Drug</topic><topic>Imidazoles - pharmacology</topic><topic>Male</topic><topic>Manganese - metabolism</topic><topic>Muscarinic Agonists - pharmacology</topic><topic>Nickel - metabolism</topic><topic>Nickel - pharmacology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Receptors, Purinergic - metabolism</topic><topic>Second Messenger Systems - physiology</topic><topic>Submandibular Gland - metabolism</topic><topic>Thionucleotides - pharmacology</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chaïb, N</creatorcontrib><creatorcontrib>Kabré, E</creatorcontrib><creatorcontrib>Métioui, M</creatorcontrib><creatorcontrib>Alzola, E</creatorcontrib><creatorcontrib>Dantinne, C</creatorcontrib><creatorcontrib>Marino, A</creatorcontrib><creatorcontrib>Dehaye, J P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell calcium (Edinburgh)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chaïb, N</au><au>Kabré, E</au><au>Métioui, M</au><au>Alzola, E</au><au>Dantinne, C</au><au>Marino, A</au><au>Dehaye, J P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential sensitivity to nickel and SK&F96365 of second messenger-operated and receptor-operated calcium channels in rat submandibular ductal cells</atitle><jtitle>Cell calcium (Edinburgh)</jtitle><addtitle>Cell Calcium</addtitle><date>1998-06</date><risdate>1998</risdate><volume>23</volume><issue>6</issue><spage>395</spage><epage>404</epage><pages>395-404</pages><issn>0143-4160</issn><abstract>The intracellular concentration of calcium ([Ca2+]i) of rat submandibular ductal cells was measured with the intracellular fluorescent dye Fura-2. Carbachol (100 microM) and ATP (1 mM) both increased the [Ca2+]i. The late response to ATP was blocked by 0.5 mM Ni2+. This concentration of Ni2+ also blocked the increase of the [Ca2+]i and the uptake of manganese and calcium in response to 2'- and 3'-O-(4-benzoylbenzoyl) adenosine 5'-triphosphate (BzATP, 100 microM), a specific agonist of P2X receptors from salivary glands. The increase of the [Ca2+]i in response to 2-methylthioadenosine 5'-triphosphate (2-MeSATP, 100 microM) a specific P2Y agonist in salivary glands or to a muscarinic agonist (carbachol) was not affected by 0.5 mM Ni2+. Only higher concentrations of Ni2+ (in the millimolar range) inhibited the uptake of extracellular calcium in response to carbachol. SK&F96365, a blocker of store-operated calcium channels, inhibited the uptake of extracellular calcium in response to carbachol without affecting the response to BzATP. It is concluded that at low concentrations (below 0.5 mM), Ni2+ inhibits the non-specific cation channel coupled to P2X receptors. The uptake of extracellular calcium by store-operated calcium channels is inhibited by higher concentrations of Ni2+ and by SK&F96365.</abstract><cop>Netherlands</cop><pmid>9924631</pmid><tpages>10</tpages></addata></record> |
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| ispartof | Cell calcium (Edinburgh), 1998-06, Vol.23 (6), p.395-404 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Adenosine Triphosphate - analogs & derivatives Adenosine Triphosphate - pharmacology Affinity Labels Animals Calcium - metabolism Calcium Channel Blockers - metabolism Calcium Channels - metabolism Calcium Chloride - pharmacology Carbachol - pharmacology Dose-Response Relationship, Drug Imidazoles - pharmacology Male Manganese - metabolism Muscarinic Agonists - pharmacology Nickel - metabolism Nickel - pharmacology Rats Rats, Wistar Receptors, Purinergic - metabolism Second Messenger Systems - physiology Submandibular Gland - metabolism Thionucleotides - pharmacology Time Factors |
| title | Differential sensitivity to nickel and SK&F96365 of second messenger-operated and receptor-operated calcium channels in rat submandibular ductal cells |
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