Cellular methods in cartilage research: Primary human chondrocytes in culture and chondrogenesis in human bone marrow stem cells
Work in our laboratory has focused on the in vitro culture of both human articular chondrocytes and human mesenchymal stem cells to understand what controls their ability to synthesise an appropriate cartilage-like extracellular matrix containing a predominantly collagen type II fibrillar network em...
Gespeichert in:
| Veröffentlicht in: | Methods (San Diego, Calif.) Calif.), 2008-05, Vol.45 (1), p.2-9 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 9 |
|---|---|
| container_issue | 1 |
| container_start_page | 2 |
| container_title | Methods (San Diego, Calif.) |
| container_volume | 45 |
| creator | Tew, Simon R. Murdoch, Alan D. Rauchenberg, Richard P. Hardingham, Timothy E. |
| description | Work in our laboratory has focused on the in vitro culture of both human articular chondrocytes and human mesenchymal stem cells to understand what controls their ability to synthesise an appropriate cartilage-like extracellular matrix containing a predominantly collagen type II fibrillar network embedded in an aggrecan-rich ECM. This review focuses on the methodologies that we have found to be successful with cartilage and bone marrow sources of human cells and comments on the many factors which may enable improved phenotypic performance once the cells are in a fully chondrogenic environment. |
| doi_str_mv | 10.1016/j.ymeth.2008.01.006 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69155404</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1046202308000224</els_id><sourcerecordid>1038593066</sourcerecordid><originalsourceid>FETCH-LOGICAL-c456t-62aa3e1bf36e13f4a585a8b999bec225919924dad52b39fba2916e020cb570993</originalsourceid><addsrcrecordid>eNp9kU9r3DAQxUVoadK0nyAQdCq52B1JltYK5BCW_oNAe2jPQpbHWS22lUh2w9760SvHW3rLSYL5vfeGeYRcMCgZMPVxXx4GnHYlB6hLYCWAOiFnDLQsNBPwavlXquDAxSl5m9IeABjf1G_IKaurim8AzsifLfb93NtIF6_QJupH6mycfG_vkUZMaKPbXdMf0Q82HuhuHmwmdmFsY3CHCVfF3E9zRGrH9t_sHkdM_nm6apowIs0eMTzRNOFAXY5O78jrzvYJ3x_fc_Lr86ef26_F3fcv37a3d4WrpJoKxa0VyJpOKGSiq6yspa0brXWDjnOpmda8am0reSN011iumULg4Bq5Aa3FOfmw-j7E8Dhjmszg07KBHTHMySjNpKygyuDViyADUUstQKmMihV1MaQUsTMP65UyZJaOzN48d2SWjgwwkzvKqstjwNwM2P7XHEvJwM0KYL7Hb4_RJOdxdNj6iG4ybfAvBvwF3huloA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1038593066</pqid></control><display><type>article</type><title>Cellular methods in cartilage research: Primary human chondrocytes in culture and chondrogenesis in human bone marrow stem cells</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Tew, Simon R. ; Murdoch, Alan D. ; Rauchenberg, Richard P. ; Hardingham, Timothy E.</creator><creatorcontrib>Tew, Simon R. ; Murdoch, Alan D. ; Rauchenberg, Richard P. ; Hardingham, Timothy E.</creatorcontrib><description>Work in our laboratory has focused on the in vitro culture of both human articular chondrocytes and human mesenchymal stem cells to understand what controls their ability to synthesise an appropriate cartilage-like extracellular matrix containing a predominantly collagen type II fibrillar network embedded in an aggrecan-rich ECM. This review focuses on the methodologies that we have found to be successful with cartilage and bone marrow sources of human cells and comments on the many factors which may enable improved phenotypic performance once the cells are in a fully chondrogenic environment.</description><identifier>ISSN: 1046-2023</identifier><identifier>EISSN: 1095-9130</identifier><identifier>DOI: 10.1016/j.ymeth.2008.01.006</identifier><identifier>PMID: 18442700</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Bone marrow ; Bone Marrow Cells - cytology ; Cartilage ; Cartilage - cytology ; Cell culture ; Cell Culture Techniques - methods ; Chondrocytes ; Chondrocytes - cytology ; Chondrogenesis ; Chondrogenesis - drug effects ; Collagen (type II) ; Extracellular matrix ; Gene Expression Regulation ; High Mobility Group Proteins - genetics ; High Mobility Group Proteins - pharmacology ; Humans ; Mesenchymal Stromal Cells - cytology ; Mesenchyme ; SOX9 ; SOX9 Transcription Factor ; Stem cells ; Tissue engineering ; Transcription Factors - genetics ; Transcription Factors - pharmacology ; Transwell</subject><ispartof>Methods (San Diego, Calif.), 2008-05, Vol.45 (1), p.2-9</ispartof><rights>2008 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c456t-62aa3e1bf36e13f4a585a8b999bec225919924dad52b39fba2916e020cb570993</citedby><cites>FETCH-LOGICAL-c456t-62aa3e1bf36e13f4a585a8b999bec225919924dad52b39fba2916e020cb570993</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1046202308000224$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18442700$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tew, Simon R.</creatorcontrib><creatorcontrib>Murdoch, Alan D.</creatorcontrib><creatorcontrib>Rauchenberg, Richard P.</creatorcontrib><creatorcontrib>Hardingham, Timothy E.</creatorcontrib><title>Cellular methods in cartilage research: Primary human chondrocytes in culture and chondrogenesis in human bone marrow stem cells</title><title>Methods (San Diego, Calif.)</title><addtitle>Methods</addtitle><description>Work in our laboratory has focused on the in vitro culture of both human articular chondrocytes and human mesenchymal stem cells to understand what controls their ability to synthesise an appropriate cartilage-like extracellular matrix containing a predominantly collagen type II fibrillar network embedded in an aggrecan-rich ECM. This review focuses on the methodologies that we have found to be successful with cartilage and bone marrow sources of human cells and comments on the many factors which may enable improved phenotypic performance once the cells are in a fully chondrogenic environment.</description><subject>Bone marrow</subject><subject>Bone Marrow Cells - cytology</subject><subject>Cartilage</subject><subject>Cartilage - cytology</subject><subject>Cell culture</subject><subject>Cell Culture Techniques - methods</subject><subject>Chondrocytes</subject><subject>Chondrocytes - cytology</subject><subject>Chondrogenesis</subject><subject>Chondrogenesis - drug effects</subject><subject>Collagen (type II)</subject><subject>Extracellular matrix</subject><subject>Gene Expression Regulation</subject><subject>High Mobility Group Proteins - genetics</subject><subject>High Mobility Group Proteins - pharmacology</subject><subject>Humans</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchyme</subject><subject>SOX9</subject><subject>SOX9 Transcription Factor</subject><subject>Stem cells</subject><subject>Tissue engineering</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - pharmacology</subject><subject>Transwell</subject><issn>1046-2023</issn><issn>1095-9130</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9r3DAQxUVoadK0nyAQdCq52B1JltYK5BCW_oNAe2jPQpbHWS22lUh2w9760SvHW3rLSYL5vfeGeYRcMCgZMPVxXx4GnHYlB6hLYCWAOiFnDLQsNBPwavlXquDAxSl5m9IeABjf1G_IKaurim8AzsifLfb93NtIF6_QJupH6mycfG_vkUZMaKPbXdMf0Q82HuhuHmwmdmFsY3CHCVfF3E9zRGrH9t_sHkdM_nm6apowIs0eMTzRNOFAXY5O78jrzvYJ3x_fc_Lr86ef26_F3fcv37a3d4WrpJoKxa0VyJpOKGSiq6yspa0brXWDjnOpmda8am0reSN011iumULg4Bq5Aa3FOfmw-j7E8Dhjmszg07KBHTHMySjNpKygyuDViyADUUstQKmMihV1MaQUsTMP65UyZJaOzN48d2SWjgwwkzvKqstjwNwM2P7XHEvJwM0KYL7Hb4_RJOdxdNj6iG4ybfAvBvwF3huloA</recordid><startdate>200805</startdate><enddate>200805</enddate><creator>Tew, Simon R.</creator><creator>Murdoch, Alan D.</creator><creator>Rauchenberg, Richard P.</creator><creator>Hardingham, Timothy E.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200805</creationdate><title>Cellular methods in cartilage research: Primary human chondrocytes in culture and chondrogenesis in human bone marrow stem cells</title><author>Tew, Simon R. ; Murdoch, Alan D. ; Rauchenberg, Richard P. ; Hardingham, Timothy E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-62aa3e1bf36e13f4a585a8b999bec225919924dad52b39fba2916e020cb570993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Bone marrow</topic><topic>Bone Marrow Cells - cytology</topic><topic>Cartilage</topic><topic>Cartilage - cytology</topic><topic>Cell culture</topic><topic>Cell Culture Techniques - methods</topic><topic>Chondrocytes</topic><topic>Chondrocytes - cytology</topic><topic>Chondrogenesis</topic><topic>Chondrogenesis - drug effects</topic><topic>Collagen (type II)</topic><topic>Extracellular matrix</topic><topic>Gene Expression Regulation</topic><topic>High Mobility Group Proteins - genetics</topic><topic>High Mobility Group Proteins - pharmacology</topic><topic>Humans</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mesenchyme</topic><topic>SOX9</topic><topic>SOX9 Transcription Factor</topic><topic>Stem cells</topic><topic>Tissue engineering</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - pharmacology</topic><topic>Transwell</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tew, Simon R.</creatorcontrib><creatorcontrib>Murdoch, Alan D.</creatorcontrib><creatorcontrib>Rauchenberg, Richard P.</creatorcontrib><creatorcontrib>Hardingham, Timothy E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Methods (San Diego, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tew, Simon R.</au><au>Murdoch, Alan D.</au><au>Rauchenberg, Richard P.</au><au>Hardingham, Timothy E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cellular methods in cartilage research: Primary human chondrocytes in culture and chondrogenesis in human bone marrow stem cells</atitle><jtitle>Methods (San Diego, Calif.)</jtitle><addtitle>Methods</addtitle><date>2008-05</date><risdate>2008</risdate><volume>45</volume><issue>1</issue><spage>2</spage><epage>9</epage><pages>2-9</pages><issn>1046-2023</issn><eissn>1095-9130</eissn><abstract>Work in our laboratory has focused on the in vitro culture of both human articular chondrocytes and human mesenchymal stem cells to understand what controls their ability to synthesise an appropriate cartilage-like extracellular matrix containing a predominantly collagen type II fibrillar network embedded in an aggrecan-rich ECM. This review focuses on the methodologies that we have found to be successful with cartilage and bone marrow sources of human cells and comments on the many factors which may enable improved phenotypic performance once the cells are in a fully chondrogenic environment.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>18442700</pmid><doi>10.1016/j.ymeth.2008.01.006</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1046-2023 |
| ispartof | Methods (San Diego, Calif.), 2008-05, Vol.45 (1), p.2-9 |
| issn | 1046-2023 1095-9130 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69155404 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Bone marrow Bone Marrow Cells - cytology Cartilage Cartilage - cytology Cell culture Cell Culture Techniques - methods Chondrocytes Chondrocytes - cytology Chondrogenesis Chondrogenesis - drug effects Collagen (type II) Extracellular matrix Gene Expression Regulation High Mobility Group Proteins - genetics High Mobility Group Proteins - pharmacology Humans Mesenchymal Stromal Cells - cytology Mesenchyme SOX9 SOX9 Transcription Factor Stem cells Tissue engineering Transcription Factors - genetics Transcription Factors - pharmacology Transwell |
| title | Cellular methods in cartilage research: Primary human chondrocytes in culture and chondrogenesis in human bone marrow stem cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-26T09%3A08%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cellular%20methods%20in%20cartilage%20research:%20Primary%20human%20chondrocytes%20in%20culture%20and%20chondrogenesis%20in%20human%20bone%20marrow%20stem%20cells&rft.jtitle=Methods%20(San%20Diego,%20Calif.)&rft.au=Tew,%20Simon%20R.&rft.date=2008-05&rft.volume=45&rft.issue=1&rft.spage=2&rft.epage=9&rft.pages=2-9&rft.issn=1046-2023&rft.eissn=1095-9130&rft_id=info:doi/10.1016/j.ymeth.2008.01.006&rft_dat=%3Cproquest_cross%3E1038593066%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1038593066&rft_id=info:pmid/18442700&rft_els_id=S1046202308000224&rfr_iscdi=true |