Development of liquid chromatographic methods for the determination of phytosterols in Standard Reference Materials containing saw palmetto
Liquid chromatographic (LC) methods using atmospheric pressure chemical ionization/mass spectrometric (APCI–MS) detection were developed for the separation and analysis of the phytosterols campesterol, cycloartenol, lupenone, lupeol, β-sitosterol, and stigmasterol. Brassicasterol and cholesterol wer...
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| Veröffentlicht in: | Journal of Chromatography A 2008-05, Vol.1192 (1), p.74-80 |
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| container_title | Journal of Chromatography A |
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| creator | Bedner, Mary Schantz, Michele M. Sander, Lane C. Sharpless, Katherine E. |
| description | Liquid chromatographic (LC) methods using atmospheric pressure chemical ionization/mass spectrometric (APCI–MS) detection were developed for the separation and analysis of the phytosterols campesterol, cycloartenol, lupenone, lupeol, β-sitosterol, and stigmasterol. Brassicasterol and cholesterol were also included for investigation as internal standards. The methods were used to identify and quantify the phytosterols in each of two
Serenoa repens (saw palmetto) Standard Reference Materials (SRMs) developed by the National Institute of Standards and Technology (NIST). Values obtained by LC–MS were compared to those obtained using the more traditional approach of gas chromatography with flame ionization detection. This is the first reported use of LC–MS to determine phytosterols in saw palmetto dietary supplement materials. |
| doi_str_mv | 10.1016/j.chroma.2008.03.020 |
| format | Article |
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Serenoa repens (saw palmetto) Standard Reference Materials (SRMs) developed by the National Institute of Standards and Technology (NIST). Values obtained by LC–MS were compared to those obtained using the more traditional approach of gas chromatography with flame ionization detection. This is the first reported use of LC–MS to determine phytosterols in saw palmetto dietary supplement materials.</description><identifier>ISSN: 0021-9673</identifier><identifier>DOI: 10.1016/j.chroma.2008.03.020</identifier><identifier>PMID: 18374927</identifier><identifier>CODEN: JOCRAM</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>atmospheric pressure chemical ionization ; beta-sitosterol ; Biological and medical sciences ; campesterol ; Chromatography, Liquid - methods ; cycloartenol ; Dietary supplements ; Food industries ; Fundamental and applied biological sciences. Psychology ; Liquid chromatography ; lupenone ; lupeol ; Mass Spectrometry ; Phytosterols ; Phytosterols - analysis ; Phytosterols - isolation & purification ; Reference materials ; Reference Standards ; Research and development. New food products, dietetic foods and beverages ; Saw palmetto ; Serenoa - chemistry ; Serenoa repens ; stigmasterol</subject><ispartof>Journal of Chromatography A, 2008-05, Vol.1192 (1), p.74-80</ispartof><rights>2008</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c414t-1c5d8496f86829c99436fd571fd60e0deba90b9624febdb19d1235e060c416eb3</citedby><cites>FETCH-LOGICAL-c414t-1c5d8496f86829c99436fd571fd60e0deba90b9624febdb19d1235e060c416eb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S002196730800486X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20332577$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18374927$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bedner, Mary</creatorcontrib><creatorcontrib>Schantz, Michele M.</creatorcontrib><creatorcontrib>Sander, Lane C.</creatorcontrib><creatorcontrib>Sharpless, Katherine E.</creatorcontrib><title>Development of liquid chromatographic methods for the determination of phytosterols in Standard Reference Materials containing saw palmetto</title><title>Journal of Chromatography A</title><addtitle>J Chromatogr A</addtitle><description>Liquid chromatographic (LC) methods using atmospheric pressure chemical ionization/mass spectrometric (APCI–MS) detection were developed for the separation and analysis of the phytosterols campesterol, cycloartenol, lupenone, lupeol, β-sitosterol, and stigmasterol. Brassicasterol and cholesterol were also included for investigation as internal standards. The methods were used to identify and quantify the phytosterols in each of two
Serenoa repens (saw palmetto) Standard Reference Materials (SRMs) developed by the National Institute of Standards and Technology (NIST). Values obtained by LC–MS were compared to those obtained using the more traditional approach of gas chromatography with flame ionization detection. This is the first reported use of LC–MS to determine phytosterols in saw palmetto dietary supplement materials.</description><subject>atmospheric pressure chemical ionization</subject><subject>beta-sitosterol</subject><subject>Biological and medical sciences</subject><subject>campesterol</subject><subject>Chromatography, Liquid - methods</subject><subject>cycloartenol</subject><subject>Dietary supplements</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Liquid chromatography</subject><subject>lupenone</subject><subject>lupeol</subject><subject>Mass Spectrometry</subject><subject>Phytosterols</subject><subject>Phytosterols - analysis</subject><subject>Phytosterols - isolation & purification</subject><subject>Reference materials</subject><subject>Reference Standards</subject><subject>Research and development. New food products, dietetic foods and beverages</subject><subject>Saw palmetto</subject><subject>Serenoa - chemistry</subject><subject>Serenoa repens</subject><subject>stigmasterol</subject><issn>0021-9673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1u1DAUhbMA0VJ4AwTewG7CtfPrDRIqFJCKkChdW459PfEosVPbU9Rn4KXrKCPYsbLk851j6yuKVxRKCrR9fyjVGPwsSwbQl1CVwOBJcQ7A6I63XXVWPI_xAEA76Niz4oz2VVdz1p0Xfz7hPU5-mdEl4g2Z7N3RarLNJb8PchmtIjOm0etIjA8kjUg0JgyzdTJZ79beMj4kH_OlnyKxjtwk6bQMmvxEgwGdQvJd5tjKnCvvkrTOuj2J8jdZ5JT3k39RPDU5xpen86K4vfr86_Lr7vrHl2-XH693qqZ12lHV6L7mrenbnnHFeV21RjcdNboFBI2D5DDwltUGBz1QrimrGoQWcr_Foboo3m27S_B3R4xJzDYqnCbp0B-jaDltWANVBusNVMHHGNCIJdhZhgdBQazixUFspsQqXkAlsvhce33aPw4z6n-lk_UMvD0BMio5mSCdsvEvlycq1nQr92bjjPRC7kNmbm8Y0Co_1vOurzPxYSMw-7q3GERUdrWtbUCVhPb2_399BDjcsiY</recordid><startdate>20080523</startdate><enddate>20080523</enddate><creator>Bedner, Mary</creator><creator>Schantz, Michele M.</creator><creator>Sander, Lane C.</creator><creator>Sharpless, Katherine E.</creator><general>Elsevier B.V</general><general>Amsterdam; New York: Elsevier</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080523</creationdate><title>Development of liquid chromatographic methods for the determination of phytosterols in Standard Reference Materials containing saw palmetto</title><author>Bedner, Mary ; Schantz, Michele M. ; Sander, Lane C. ; Sharpless, Katherine E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c414t-1c5d8496f86829c99436fd571fd60e0deba90b9624febdb19d1235e060c416eb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>atmospheric pressure chemical ionization</topic><topic>beta-sitosterol</topic><topic>Biological and medical sciences</topic><topic>campesterol</topic><topic>Chromatography, Liquid - methods</topic><topic>cycloartenol</topic><topic>Dietary supplements</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Liquid chromatography</topic><topic>lupenone</topic><topic>lupeol</topic><topic>Mass Spectrometry</topic><topic>Phytosterols</topic><topic>Phytosterols - analysis</topic><topic>Phytosterols - isolation & purification</topic><topic>Reference materials</topic><topic>Reference Standards</topic><topic>Research and development. New food products, dietetic foods and beverages</topic><topic>Saw palmetto</topic><topic>Serenoa - chemistry</topic><topic>Serenoa repens</topic><topic>stigmasterol</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bedner, Mary</creatorcontrib><creatorcontrib>Schantz, Michele M.</creatorcontrib><creatorcontrib>Sander, Lane C.</creatorcontrib><creatorcontrib>Sharpless, Katherine E.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bedner, Mary</au><au>Schantz, Michele M.</au><au>Sander, Lane C.</au><au>Sharpless, Katherine E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of liquid chromatographic methods for the determination of phytosterols in Standard Reference Materials containing saw palmetto</atitle><jtitle>Journal of Chromatography A</jtitle><addtitle>J Chromatogr A</addtitle><date>2008-05-23</date><risdate>2008</risdate><volume>1192</volume><issue>1</issue><spage>74</spage><epage>80</epage><pages>74-80</pages><issn>0021-9673</issn><coden>JOCRAM</coden><abstract>Liquid chromatographic (LC) methods using atmospheric pressure chemical ionization/mass spectrometric (APCI–MS) detection were developed for the separation and analysis of the phytosterols campesterol, cycloartenol, lupenone, lupeol, β-sitosterol, and stigmasterol. Brassicasterol and cholesterol were also included for investigation as internal standards. The methods were used to identify and quantify the phytosterols in each of two
Serenoa repens (saw palmetto) Standard Reference Materials (SRMs) developed by the National Institute of Standards and Technology (NIST). Values obtained by LC–MS were compared to those obtained using the more traditional approach of gas chromatography with flame ionization detection. This is the first reported use of LC–MS to determine phytosterols in saw palmetto dietary supplement materials.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>18374927</pmid><doi>10.1016/j.chroma.2008.03.020</doi><tpages>7</tpages></addata></record> |
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| subjects | atmospheric pressure chemical ionization beta-sitosterol Biological and medical sciences campesterol Chromatography, Liquid - methods cycloartenol Dietary supplements Food industries Fundamental and applied biological sciences. Psychology Liquid chromatography lupenone lupeol Mass Spectrometry Phytosterols Phytosterols - analysis Phytosterols - isolation & purification Reference materials Reference Standards Research and development. New food products, dietetic foods and beverages Saw palmetto Serenoa - chemistry Serenoa repens stigmasterol |
| title | Development of liquid chromatographic methods for the determination of phytosterols in Standard Reference Materials containing saw palmetto |
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