Usefulness of enzyme linked immunosorbent assays species specific in the detection of Chlamydia trachomatis and Chlamydophila pneumoniae IgG antibodies in patients with genital infections or respiratory tract infections
Chlamydia trachomatis (Ct) and Chlamydophila pneumoniae (Cpn) are obligate intracellular bacteria causing genital tract infections (GTI) and respiratory tract infections (RTI), respectively. Antigenic cross-reactivity between the two species may complicate serologic diagnosis. In this study, we comp...
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| description | Chlamydia trachomatis (Ct) and
Chlamydophila pneumoniae (Cpn) are obligate intracellular bacteria causing genital tract infections (GTI) and respiratory tract infections (RTI), respectively. Antigenic cross-reactivity between the two species may complicate serologic diagnosis. In this study, we compared the performance of two ELISA tests in relation to microimmunofluorescence (MIF) for the detection of Ct and Cpn IgG antibodies. We also explored the degree of cross-reactivity by ELISA and MIF. Among 278 positive sera for Cpn and/or Ct IgG antibodies in the MIF, 153 were from patients with GTI and 125 were from patients with RTI. These sera were tested by our in house MIF test and by two commercial ELISA: SeroCP and SeroCT for the detection of anti-Cpn IgG antibodies and anti-Ct IgG antibodies, respectively. In sera from patients with RTI, correlation between MIF and SeroCP was 92%. The specificity of this test was 38.5%. In fact, among the 140 sera from patients with GTI and that cross-reacted in MIF, only six were confirmed by the two ELISA tests as having IgG antibodies to Ct. The correlation between MIF and SeroCT was 80%. The specificity of this test was 100%. Indeed, among the 65 sera from patients with RTI with cross-reactions in MIF, 30 sera showed a negative SeroCT test. SeroCT was highly specific and could diminish considerably the extent of cross-reactions. Whilst, SeroCP test was not specific enough to distinguish between the presence of IgG antibodies and Cpn or Ct.
Chlamydia trachomatis (Ct) et
Chlamydophila pneumoniae (Cpn) sont deux bactéries intracellulaires obligatoires causant des infections génitales (GTI) et des infections respiratoires (RTI) respectivement. La réactivité croisée entre ces deux espèces peut compliquer le diagnostic sérologique. Dans notre étude, nous avons comparé les performances de deux tests Elisa à la micro-immunofluorescence (MIF) pour la détection des anticorps anti-IgG de Ct et de Cpn. Nous avons exploré le degré de réactivité croisée entre les deux espèces par Elisa et MIF. Nous avons inclus 153 sérums de patients consultant pour GTI et 125 sérums de patients consultant pour RTI. Ces patients avaient des évidences sérologiques d’une infection à
Chlamydia par MIF (test maison). Tous les sérums ont été aussi testés par deux tests Elisa commerciaux : SeroCP et SeroCT pour la détection des anticorps anti-Cpn IgG et anti-Ct IgG, respectivement. La corrélation entre la MIF et SeroCP était de 92 % pour les sérums de pa |
| doi_str_mv | 10.1016/j.patbio.2007.09.020 |
| format | Article |
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Chlamydophila pneumoniae (Cpn) are obligate intracellular bacteria causing genital tract infections (GTI) and respiratory tract infections (RTI), respectively. Antigenic cross-reactivity between the two species may complicate serologic diagnosis. In this study, we compared the performance of two ELISA tests in relation to microimmunofluorescence (MIF) for the detection of Ct and Cpn IgG antibodies. We also explored the degree of cross-reactivity by ELISA and MIF. Among 278 positive sera for Cpn and/or Ct IgG antibodies in the MIF, 153 were from patients with GTI and 125 were from patients with RTI. These sera were tested by our in house MIF test and by two commercial ELISA: SeroCP and SeroCT for the detection of anti-Cpn IgG antibodies and anti-Ct IgG antibodies, respectively. In sera from patients with RTI, correlation between MIF and SeroCP was 92%. The specificity of this test was 38.5%. In fact, among the 140 sera from patients with GTI and that cross-reacted in MIF, only six were confirmed by the two ELISA tests as having IgG antibodies to Ct. The correlation between MIF and SeroCT was 80%. The specificity of this test was 100%. Indeed, among the 65 sera from patients with RTI with cross-reactions in MIF, 30 sera showed a negative SeroCT test. SeroCT was highly specific and could diminish considerably the extent of cross-reactions. Whilst, SeroCP test was not specific enough to distinguish between the presence of IgG antibodies and Cpn or Ct.
Chlamydia trachomatis (Ct) et
Chlamydophila pneumoniae (Cpn) sont deux bactéries intracellulaires obligatoires causant des infections génitales (GTI) et des infections respiratoires (RTI) respectivement. La réactivité croisée entre ces deux espèces peut compliquer le diagnostic sérologique. Dans notre étude, nous avons comparé les performances de deux tests Elisa à la micro-immunofluorescence (MIF) pour la détection des anticorps anti-IgG de Ct et de Cpn. Nous avons exploré le degré de réactivité croisée entre les deux espèces par Elisa et MIF. Nous avons inclus 153 sérums de patients consultant pour GTI et 125 sérums de patients consultant pour RTI. Ces patients avaient des évidences sérologiques d’une infection à
Chlamydia par MIF (test maison). Tous les sérums ont été aussi testés par deux tests Elisa commerciaux : SeroCP et SeroCT pour la détection des anticorps anti-Cpn IgG et anti-Ct IgG, respectivement. La corrélation entre la MIF et SeroCP était de 92 % pour les sérums de patients avec RTI. La spécificité de ce test était de 38,5 %. La corrélation entre la MIF et SeroCT était de 80 %. La spécificité de ce test était de 100 %. SeroCT était, contrairement à SeroCP, hautement spécifique et a permis la diminution considérable des réactivités croisées.</description><identifier>ISSN: 0369-8114</identifier><identifier>EISSN: 1768-3114</identifier><identifier>DOI: 10.1016/j.patbio.2007.09.020</identifier><identifier>PMID: 18178034</identifier><language>eng</language><publisher>Paris: Elsevier SAS</publisher><subject>Antibodies, Fungal - blood ; Bacterial diseases ; Bacterial diseases of the genital system ; Biological and medical sciences ; Chlamydia Infections - diagnosis ; Chlamydia Infections - immunology ; Chlamydia trachomatis ; Chlamydia trachomatis - immunology ; Chlamydia trachomatis - isolation & purification ; Chlamydophila Infections - diagnosis ; Chlamydophila Infections - immunology ; Chlamydophila pneumoniae ; Chlamydophila pneumoniae - immunology ; Chlamydophila pneumoniae - isolation & purification ; Cross Reactions ; Diagnosis, Differential ; Enzyme-Linked Immunosorbent Assay - methods ; Female ; Fluorescent Antibody Technique ; Genital Diseases, Female - immunology ; Genital Diseases, Female - microbiology ; Genital Diseases, Male - immunology ; Genital Diseases, Male - microbiology ; Human bacterial diseases ; Humans ; Immunoglobulin G - immunology ; Infectious diseases ; Investigative techniques, diagnostic techniques (general aspects) ; Male ; Medical sciences ; Respiratory Tract Infections - diagnosis ; Réactions croisées ; Serology ; Specificity ; Spécificité ; Sérologie</subject><ispartof>Pathologie biologie (Paris), 2008-05, Vol.56 (3), p.143-147</ispartof><rights>2007 Elsevier Masson SAS</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-a07080997d3ad898e7d282668fb158d7410b7f8af04b6f027c620b3e1035e2d53</citedby><cites>FETCH-LOGICAL-c390t-a07080997d3ad898e7d282668fb158d7410b7f8af04b6f027c620b3e1035e2d53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.patbio.2007.09.020$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20282091$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18178034$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Frikha-Gargouri, O.</creatorcontrib><creatorcontrib>Znazen, A.</creatorcontrib><creatorcontrib>Gdoura, R.</creatorcontrib><creatorcontrib>Gargouri, B.</creatorcontrib><creatorcontrib>Arab, N. Ben</creatorcontrib><creatorcontrib>Jemaa, M. Ben</creatorcontrib><creatorcontrib>Hammami, A.</creatorcontrib><title>Usefulness of enzyme linked immunosorbent assays species specific in the detection of Chlamydia trachomatis and Chlamydophila pneumoniae IgG antibodies in patients with genital infections or respiratory tract infections</title><title>Pathologie biologie (Paris)</title><addtitle>Pathol Biol (Paris)</addtitle><description>Chlamydia trachomatis (Ct) and
Chlamydophila pneumoniae (Cpn) are obligate intracellular bacteria causing genital tract infections (GTI) and respiratory tract infections (RTI), respectively. Antigenic cross-reactivity between the two species may complicate serologic diagnosis. In this study, we compared the performance of two ELISA tests in relation to microimmunofluorescence (MIF) for the detection of Ct and Cpn IgG antibodies. We also explored the degree of cross-reactivity by ELISA and MIF. Among 278 positive sera for Cpn and/or Ct IgG antibodies in the MIF, 153 were from patients with GTI and 125 were from patients with RTI. These sera were tested by our in house MIF test and by two commercial ELISA: SeroCP and SeroCT for the detection of anti-Cpn IgG antibodies and anti-Ct IgG antibodies, respectively. In sera from patients with RTI, correlation between MIF and SeroCP was 92%. The specificity of this test was 38.5%. In fact, among the 140 sera from patients with GTI and that cross-reacted in MIF, only six were confirmed by the two ELISA tests as having IgG antibodies to Ct. The correlation between MIF and SeroCT was 80%. The specificity of this test was 100%. Indeed, among the 65 sera from patients with RTI with cross-reactions in MIF, 30 sera showed a negative SeroCT test. SeroCT was highly specific and could diminish considerably the extent of cross-reactions. Whilst, SeroCP test was not specific enough to distinguish between the presence of IgG antibodies and Cpn or Ct.
Chlamydia trachomatis (Ct) et
Chlamydophila pneumoniae (Cpn) sont deux bactéries intracellulaires obligatoires causant des infections génitales (GTI) et des infections respiratoires (RTI) respectivement. La réactivité croisée entre ces deux espèces peut compliquer le diagnostic sérologique. Dans notre étude, nous avons comparé les performances de deux tests Elisa à la micro-immunofluorescence (MIF) pour la détection des anticorps anti-IgG de Ct et de Cpn. Nous avons exploré le degré de réactivité croisée entre les deux espèces par Elisa et MIF. Nous avons inclus 153 sérums de patients consultant pour GTI et 125 sérums de patients consultant pour RTI. Ces patients avaient des évidences sérologiques d’une infection à
Chlamydia par MIF (test maison). Tous les sérums ont été aussi testés par deux tests Elisa commerciaux : SeroCP et SeroCT pour la détection des anticorps anti-Cpn IgG et anti-Ct IgG, respectivement. La corrélation entre la MIF et SeroCP était de 92 % pour les sérums de patients avec RTI. La spécificité de ce test était de 38,5 %. La corrélation entre la MIF et SeroCT était de 80 %. La spécificité de ce test était de 100 %. SeroCT était, contrairement à SeroCP, hautement spécifique et a permis la diminution considérable des réactivités croisées.</description><subject>Antibodies, Fungal - blood</subject><subject>Bacterial diseases</subject><subject>Bacterial diseases of the genital system</subject><subject>Biological and medical sciences</subject><subject>Chlamydia Infections - diagnosis</subject><subject>Chlamydia Infections - immunology</subject><subject>Chlamydia trachomatis</subject><subject>Chlamydia trachomatis - immunology</subject><subject>Chlamydia trachomatis - isolation & purification</subject><subject>Chlamydophila Infections - diagnosis</subject><subject>Chlamydophila Infections - immunology</subject><subject>Chlamydophila pneumoniae</subject><subject>Chlamydophila pneumoniae - immunology</subject><subject>Chlamydophila pneumoniae - isolation & purification</subject><subject>Cross Reactions</subject><subject>Diagnosis, Differential</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Female</subject><subject>Fluorescent Antibody Technique</subject><subject>Genital Diseases, Female - immunology</subject><subject>Genital Diseases, Female - microbiology</subject><subject>Genital Diseases, Male - immunology</subject><subject>Genital Diseases, Male - microbiology</subject><subject>Human bacterial diseases</subject><subject>Humans</subject><subject>Immunoglobulin G - immunology</subject><subject>Infectious diseases</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Respiratory Tract Infections - diagnosis</subject><subject>Réactions croisées</subject><subject>Serology</subject><subject>Specificity</subject><subject>Spécificité</subject><subject>Sérologie</subject><issn>0369-8114</issn><issn>1768-3114</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU2P0zAQhiMEYpeFf4CQL3BrGSdpPi5IqIJlpZW4sGfLscfbKYkdPA6o_FX-DC4tHydOM9I8887HWxTPJawlyOb1fj3rNFBYlwDtGvo1lPCguJRt060qKeuHxSVUTb_qcn5RPGHeA8hW1vJxcSE72XZQ1ZfFjztGt4wemUVwAv33w4RiJP8ZraBpWnzgEAf0SWhmfWDBMxrCc3RkBHmRdigsJjSJgj_qbHejng6WtEhRm12YdCIW2tvflTDvaNRi9rhMwZNGcXN_nYFEQ7BH-aya76M8mMU3Sjtxj56SHnPBnebkhaOIyDNFnUI8_BqV_qk_LR45PTI-O8er4u79u0_bD6vbj9c327e3K1P1kFYaWuig71tbadv1Hba27Mqm6dwgN51tawlD6zrtoB4aB2VrmhKGCiVUGyztproqXp105xi-LMhJTcQGx1F7DAurppcbWfdlBusTaGJgjujUHGnS8aAkqKOpaq9OpqqjqQp6lU3NbS_O-sswof3bdHYxAy_PgGajRxe1N8R_uBLyPdDLzL05cZi_8ZUwKs5WeoOWYv6ZsoH-v8lPnlzIqw</recordid><startdate>20080501</startdate><enddate>20080501</enddate><creator>Frikha-Gargouri, O.</creator><creator>Znazen, A.</creator><creator>Gdoura, R.</creator><creator>Gargouri, B.</creator><creator>Arab, N. Ben</creator><creator>Jemaa, M. Ben</creator><creator>Hammami, A.</creator><general>Elsevier SAS</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080501</creationdate><title>Usefulness of enzyme linked immunosorbent assays species specific in the detection of Chlamydia trachomatis and Chlamydophila pneumoniae IgG antibodies in patients with genital infections or respiratory tract infections</title><author>Frikha-Gargouri, O. ; Znazen, A. ; Gdoura, R. ; Gargouri, B. ; Arab, N. Ben ; Jemaa, M. Ben ; Hammami, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-a07080997d3ad898e7d282668fb158d7410b7f8af04b6f027c620b3e1035e2d53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Antibodies, Fungal - blood</topic><topic>Bacterial diseases</topic><topic>Bacterial diseases of the genital system</topic><topic>Biological and medical sciences</topic><topic>Chlamydia Infections - diagnosis</topic><topic>Chlamydia Infections - immunology</topic><topic>Chlamydia trachomatis</topic><topic>Chlamydia trachomatis - immunology</topic><topic>Chlamydia trachomatis - isolation & purification</topic><topic>Chlamydophila Infections - diagnosis</topic><topic>Chlamydophila Infections - immunology</topic><topic>Chlamydophila pneumoniae</topic><topic>Chlamydophila pneumoniae - immunology</topic><topic>Chlamydophila pneumoniae - isolation & purification</topic><topic>Cross Reactions</topic><topic>Diagnosis, Differential</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Female</topic><topic>Fluorescent Antibody Technique</topic><topic>Genital Diseases, Female - immunology</topic><topic>Genital Diseases, Female - microbiology</topic><topic>Genital Diseases, Male - immunology</topic><topic>Genital Diseases, Male - microbiology</topic><topic>Human bacterial diseases</topic><topic>Humans</topic><topic>Immunoglobulin G - immunology</topic><topic>Infectious diseases</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Respiratory Tract Infections - diagnosis</topic><topic>Réactions croisées</topic><topic>Serology</topic><topic>Specificity</topic><topic>Spécificité</topic><topic>Sérologie</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Frikha-Gargouri, O.</creatorcontrib><creatorcontrib>Znazen, A.</creatorcontrib><creatorcontrib>Gdoura, R.</creatorcontrib><creatorcontrib>Gargouri, B.</creatorcontrib><creatorcontrib>Arab, N. Ben</creatorcontrib><creatorcontrib>Jemaa, M. Ben</creatorcontrib><creatorcontrib>Hammami, A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Pathologie biologie (Paris)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Frikha-Gargouri, O.</au><au>Znazen, A.</au><au>Gdoura, R.</au><au>Gargouri, B.</au><au>Arab, N. Ben</au><au>Jemaa, M. Ben</au><au>Hammami, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Usefulness of enzyme linked immunosorbent assays species specific in the detection of Chlamydia trachomatis and Chlamydophila pneumoniae IgG antibodies in patients with genital infections or respiratory tract infections</atitle><jtitle>Pathologie biologie (Paris)</jtitle><addtitle>Pathol Biol (Paris)</addtitle><date>2008-05-01</date><risdate>2008</risdate><volume>56</volume><issue>3</issue><spage>143</spage><epage>147</epage><pages>143-147</pages><issn>0369-8114</issn><eissn>1768-3114</eissn><abstract>Chlamydia trachomatis (Ct) and
Chlamydophila pneumoniae (Cpn) are obligate intracellular bacteria causing genital tract infections (GTI) and respiratory tract infections (RTI), respectively. Antigenic cross-reactivity between the two species may complicate serologic diagnosis. In this study, we compared the performance of two ELISA tests in relation to microimmunofluorescence (MIF) for the detection of Ct and Cpn IgG antibodies. We also explored the degree of cross-reactivity by ELISA and MIF. Among 278 positive sera for Cpn and/or Ct IgG antibodies in the MIF, 153 were from patients with GTI and 125 were from patients with RTI. These sera were tested by our in house MIF test and by two commercial ELISA: SeroCP and SeroCT for the detection of anti-Cpn IgG antibodies and anti-Ct IgG antibodies, respectively. In sera from patients with RTI, correlation between MIF and SeroCP was 92%. The specificity of this test was 38.5%. In fact, among the 140 sera from patients with GTI and that cross-reacted in MIF, only six were confirmed by the two ELISA tests as having IgG antibodies to Ct. The correlation between MIF and SeroCT was 80%. The specificity of this test was 100%. Indeed, among the 65 sera from patients with RTI with cross-reactions in MIF, 30 sera showed a negative SeroCT test. SeroCT was highly specific and could diminish considerably the extent of cross-reactions. Whilst, SeroCP test was not specific enough to distinguish between the presence of IgG antibodies and Cpn or Ct.
Chlamydia trachomatis (Ct) et
Chlamydophila pneumoniae (Cpn) sont deux bactéries intracellulaires obligatoires causant des infections génitales (GTI) et des infections respiratoires (RTI) respectivement. La réactivité croisée entre ces deux espèces peut compliquer le diagnostic sérologique. Dans notre étude, nous avons comparé les performances de deux tests Elisa à la micro-immunofluorescence (MIF) pour la détection des anticorps anti-IgG de Ct et de Cpn. Nous avons exploré le degré de réactivité croisée entre les deux espèces par Elisa et MIF. Nous avons inclus 153 sérums de patients consultant pour GTI et 125 sérums de patients consultant pour RTI. Ces patients avaient des évidences sérologiques d’une infection à
Chlamydia par MIF (test maison). Tous les sérums ont été aussi testés par deux tests Elisa commerciaux : SeroCP et SeroCT pour la détection des anticorps anti-Cpn IgG et anti-Ct IgG, respectivement. La corrélation entre la MIF et SeroCP était de 92 % pour les sérums de patients avec RTI. La spécificité de ce test était de 38,5 %. La corrélation entre la MIF et SeroCT était de 80 %. La spécificité de ce test était de 100 %. SeroCT était, contrairement à SeroCP, hautement spécifique et a permis la diminution considérable des réactivités croisées.</abstract><cop>Paris</cop><pub>Elsevier SAS</pub><pmid>18178034</pmid><doi>10.1016/j.patbio.2007.09.020</doi><tpages>5</tpages></addata></record> |
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| ispartof | Pathologie biologie (Paris), 2008-05, Vol.56 (3), p.143-147 |
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| subjects | Antibodies, Fungal - blood Bacterial diseases Bacterial diseases of the genital system Biological and medical sciences Chlamydia Infections - diagnosis Chlamydia Infections - immunology Chlamydia trachomatis Chlamydia trachomatis - immunology Chlamydia trachomatis - isolation & purification Chlamydophila Infections - diagnosis Chlamydophila Infections - immunology Chlamydophila pneumoniae Chlamydophila pneumoniae - immunology Chlamydophila pneumoniae - isolation & purification Cross Reactions Diagnosis, Differential Enzyme-Linked Immunosorbent Assay - methods Female Fluorescent Antibody Technique Genital Diseases, Female - immunology Genital Diseases, Female - microbiology Genital Diseases, Male - immunology Genital Diseases, Male - microbiology Human bacterial diseases Humans Immunoglobulin G - immunology Infectious diseases Investigative techniques, diagnostic techniques (general aspects) Male Medical sciences Respiratory Tract Infections - diagnosis Réactions croisées Serology Specificity Spécificité Sérologie |
| title | Usefulness of enzyme linked immunosorbent assays species specific in the detection of Chlamydia trachomatis and Chlamydophila pneumoniae IgG antibodies in patients with genital infections or respiratory tract infections |
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