A split-protein sensor for studying protein–protein interaction in mycobacteria

The study of protein function in living cells is an essential complement to genomics, yet method development does not always keep pace with sequencing. Experimental techniques for the genus mycobacteria are relatively underdeveloped, though seventeen genomes have been sequenced. “Split-Trp” is a spl...

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Veröffentlicht in:	Journal of microbiological methods 2008-05, Vol.73 (2), p.79-84
Hauptverfasser:	O'Hare, Helen, Juillerat, Alexandre, Dianišková, Petronela, Johnsson, Kai
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacteriology Biological and medical sciences Escherichia coli Escherichia coli - genetics Escherichia coli - growth & development Fundamental and applied biological sciences. Psychology Genetic Vectors Microbiology Miscellaneous Mycobacterium smegmatis Mycobacterium smegmatis - genetics Mycobacterium smegmatis - growth & development Mycobacterium smegmatis - metabolism Plasmids Protein Binding Protein interaction Protein Interaction Mapping - methods Saccharomyces cerevisiae Split-protein sensor Tuberculosis
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