A split-protein sensor for studying protein–protein interaction in mycobacteria
The study of protein function in living cells is an essential complement to genomics, yet method development does not always keep pace with sequencing. Experimental techniques for the genus mycobacteria are relatively underdeveloped, though seventeen genomes have been sequenced. “Split-Trp” is a spl...
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| Veröffentlicht in: | Journal of microbiological methods 2008-05, Vol.73 (2), p.79-84 |
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| container_end_page | 84 |
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| container_issue | 2 |
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| container_title | Journal of microbiological methods |
| container_volume | 73 |
| creator | O'Hare, Helen Juillerat, Alexandre Dianišková, Petronela Johnsson, Kai |
| description | The study of protein function in living cells is an essential complement to genomics, yet method development does not always keep pace with sequencing. Experimental techniques for the genus mycobacteria are relatively underdeveloped, though seventeen genomes have been sequenced. “Split-Trp” is a split-protein sensor used to detect protein–protein interactions in tryptophan auxotrophic
Saccharomyces cerevisiae, but the principles behind the sensor should allow it to function in a broad range of microbial hosts. Here we introduce Split-Trp to
Escherichia coli and
Mycobacterium smegmatis and demonstrate that this system is a simple assay for protein interaction in both organisms. |
| doi_str_mv | 10.1016/j.mimet.2008.02.008 |
| format | Article |
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Saccharomyces cerevisiae, but the principles behind the sensor should allow it to function in a broad range of microbial hosts. Here we introduce Split-Trp to
Escherichia coli and
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Saccharomyces cerevisiae, but the principles behind the sensor should allow it to function in a broad range of microbial hosts. Here we introduce Split-Trp to
Escherichia coli and
Mycobacterium smegmatis and demonstrate that this system is a simple assay for protein interaction in both organisms.</description><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - growth & development</subject><subject>Fundamental and applied biological sciences. 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Saccharomyces cerevisiae, but the principles behind the sensor should allow it to function in a broad range of microbial hosts. Here we introduce Split-Trp to
Escherichia coli and
Mycobacterium smegmatis and demonstrate that this system is a simple assay for protein interaction in both organisms.</abstract><cop>Shannon</cop><pub>Elsevier B.V</pub><pmid>18387683</pmid><doi>10.1016/j.mimet.2008.02.008</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Journal of microbiological methods, 2008-05, Vol.73 (2), p.79-84 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacteriology Biological and medical sciences Escherichia coli Escherichia coli - genetics Escherichia coli - growth & development Fundamental and applied biological sciences. Psychology Genetic Vectors Microbiology Miscellaneous Mycobacterium smegmatis Mycobacterium smegmatis - genetics Mycobacterium smegmatis - growth & development Mycobacterium smegmatis - metabolism Plasmids Protein Binding Protein interaction Protein Interaction Mapping - methods Saccharomyces cerevisiae Split-protein sensor Tuberculosis |
| title | A split-protein sensor for studying protein–protein interaction in mycobacteria |
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