Evidence for a common epitope between bovine alpha-lactalbumin and beta-lactoglobulin
The aim of this work was to check and quantify any cross-reactivities among the main bovine whey proteins, utilizing purified polyclonal antibodies against bovine beta-lactoglobulin, alpha-lactalbumin and serum albumin, and to identify possible common epitope(s). Purified polyclonal anti-bovine beta...
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| Veröffentlicht in: | Biological chemistry 1998-12, Vol.379 (12), p.1453-1456 |
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| creator | Baroglio, C Giuffrida, M.G Cantisani, A Napolitano, L Bertino, E Fabris, C Conti, A |
| description | The aim of this work was to check and quantify any cross-reactivities among the main bovine whey proteins, utilizing purified polyclonal antibodies against bovine beta-lactoglobulin, alpha-lactalbumin and serum albumin, and to identify possible common epitope(s). Purified polyclonal anti-bovine beta-lactoglobulin antibodies show 10% cross-reactivity with bovine alpha-lactalbumin, both in its native and its denatured form. A continuous stretch of four amino acids common to alpha-lactalbumin and beta-lactoglobulin that might be responsible for this cross-reactivity has been identified. Cross-reactivity between this antibody and bovine serum albumin is, on the contrary, negligible. Purified polyclonal anti-bovine alpha-lactalbumin and anti-serum albumin antibodies do not cross-react. |
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Purified polyclonal anti-bovine beta-lactoglobulin antibodies show 10% cross-reactivity with bovine alpha-lactalbumin, both in its native and its denatured form. A continuous stretch of four amino acids common to alpha-lactalbumin and beta-lactoglobulin that might be responsible for this cross-reactivity has been identified. Cross-reactivity between this antibody and bovine serum albumin is, on the contrary, negligible. Purified polyclonal anti-bovine alpha-lactalbumin and anti-serum albumin antibodies do not cross-react.</description><identifier>ISSN: 1431-6730</identifier><identifier>EISSN: 1437-4315</identifier><identifier>PMID: 9894814</identifier><language>eng</language><publisher>Germany</publisher><subject>Amino Acid Sequence ; Animals ; Cattle ; cross reaction ; Cross Reactions ; Electrophoresis, Polyacrylamide Gel ; Enzyme-Linked Immunosorbent Assay ; epitopes ; Epitopes - immunology ; Lactalbumin - chemistry ; Lactalbumin - immunology ; Lactoglobulins - chemistry ; Lactoglobulins - immunology ; milk allergy ; milk proteins</subject><ispartof>Biological chemistry, 1998-12, Vol.379 (12), p.1453-1456</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9894814$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Baroglio, C</creatorcontrib><creatorcontrib>Giuffrida, M.G</creatorcontrib><creatorcontrib>Cantisani, A</creatorcontrib><creatorcontrib>Napolitano, L</creatorcontrib><creatorcontrib>Bertino, E</creatorcontrib><creatorcontrib>Fabris, C</creatorcontrib><creatorcontrib>Conti, A</creatorcontrib><title>Evidence for a common epitope between bovine alpha-lactalbumin and beta-lactoglobulin</title><title>Biological chemistry</title><addtitle>Biol Chem</addtitle><description>The aim of this work was to check and quantify any cross-reactivities among the main bovine whey proteins, utilizing purified polyclonal antibodies against bovine beta-lactoglobulin, alpha-lactalbumin and serum albumin, and to identify possible common epitope(s). Purified polyclonal anti-bovine beta-lactoglobulin antibodies show 10% cross-reactivity with bovine alpha-lactalbumin, both in its native and its denatured form. A continuous stretch of four amino acids common to alpha-lactalbumin and beta-lactoglobulin that might be responsible for this cross-reactivity has been identified. Cross-reactivity between this antibody and bovine serum albumin is, on the contrary, negligible. Purified polyclonal anti-bovine alpha-lactalbumin and anti-serum albumin antibodies do not cross-react.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Cattle</subject><subject>cross reaction</subject><subject>Cross Reactions</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>epitopes</subject><subject>Epitopes - immunology</subject><subject>Lactalbumin - chemistry</subject><subject>Lactalbumin - immunology</subject><subject>Lactoglobulins - chemistry</subject><subject>Lactoglobulins - immunology</subject><subject>milk allergy</subject><subject>milk proteins</subject><issn>1431-6730</issn><issn>1437-4315</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNotkEtPwzAQhC0EKqXwExA-cYvkd5wjqspDqsQBeo78LEaOHeKkiH9PoD3taufTambOwBIzWleMYn7-v-NK1BRdgqtSPhFCEjG6AItGNkxitgS7zSFYl4yDPg9QQZO7Lifo-jDm3kHtxm_nEtT5EJKDKvYfqorKjCrqqQsJqmT_oOMx72PWUwzpGlx4FYu7Oc0V2D1u3tfP1fb16WX9sK08oWisiKU15YxzSYi2TBjGmfaYEYGpwEgxYiy3uJZ0BppGSVxz6zzh0gpv5nArcH_82w_5a3JlbLtQjItRJZen0ooGM8EIn8HbEzjpztm2H0Knhp_21MOs3x11r3Kr9kMo7e6NIEwRaRDms81ffhhitg</recordid><startdate>19981201</startdate><enddate>19981201</enddate><creator>Baroglio, C</creator><creator>Giuffrida, M.G</creator><creator>Cantisani, A</creator><creator>Napolitano, L</creator><creator>Bertino, E</creator><creator>Fabris, C</creator><creator>Conti, A</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19981201</creationdate><title>Evidence for a common epitope between bovine alpha-lactalbumin and beta-lactoglobulin</title><author>Baroglio, C ; Giuffrida, M.G ; Cantisani, A ; Napolitano, L ; Bertino, E ; Fabris, C ; Conti, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f230t-2d3735455822bd46c454bf142613610a42cd5d178382299a8175def258d6fc143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Cattle</topic><topic>cross reaction</topic><topic>Cross Reactions</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>epitopes</topic><topic>Epitopes - immunology</topic><topic>Lactalbumin - chemistry</topic><topic>Lactalbumin - immunology</topic><topic>Lactoglobulins - chemistry</topic><topic>Lactoglobulins - immunology</topic><topic>milk allergy</topic><topic>milk proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Baroglio, C</creatorcontrib><creatorcontrib>Giuffrida, M.G</creatorcontrib><creatorcontrib>Cantisani, A</creatorcontrib><creatorcontrib>Napolitano, L</creatorcontrib><creatorcontrib>Bertino, E</creatorcontrib><creatorcontrib>Fabris, C</creatorcontrib><creatorcontrib>Conti, A</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Baroglio, C</au><au>Giuffrida, M.G</au><au>Cantisani, A</au><au>Napolitano, L</au><au>Bertino, E</au><au>Fabris, C</au><au>Conti, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evidence for a common epitope between bovine alpha-lactalbumin and beta-lactoglobulin</atitle><jtitle>Biological chemistry</jtitle><addtitle>Biol Chem</addtitle><date>1998-12-01</date><risdate>1998</risdate><volume>379</volume><issue>12</issue><spage>1453</spage><epage>1456</epage><pages>1453-1456</pages><issn>1431-6730</issn><eissn>1437-4315</eissn><abstract>The aim of this work was to check and quantify any cross-reactivities among the main bovine whey proteins, utilizing purified polyclonal antibodies against bovine beta-lactoglobulin, alpha-lactalbumin and serum albumin, and to identify possible common epitope(s). Purified polyclonal anti-bovine beta-lactoglobulin antibodies show 10% cross-reactivity with bovine alpha-lactalbumin, both in its native and its denatured form. A continuous stretch of four amino acids common to alpha-lactalbumin and beta-lactoglobulin that might be responsible for this cross-reactivity has been identified. Cross-reactivity between this antibody and bovine serum albumin is, on the contrary, negligible. Purified polyclonal anti-bovine alpha-lactalbumin and anti-serum albumin antibodies do not cross-react.</abstract><cop>Germany</cop><pmid>9894814</pmid><tpages>4</tpages></addata></record> |
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| identifier | ISSN: 1431-6730 |
| ispartof | Biological chemistry, 1998-12, Vol.379 (12), p.1453-1456 |
| issn | 1431-6730 1437-4315 |
| language | eng |
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| source | MEDLINE; De Gruyter journals |
| subjects | Amino Acid Sequence Animals Cattle cross reaction Cross Reactions Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay epitopes Epitopes - immunology Lactalbumin - chemistry Lactalbumin - immunology Lactoglobulins - chemistry Lactoglobulins - immunology milk allergy milk proteins |
| title | Evidence for a common epitope between bovine alpha-lactalbumin and beta-lactoglobulin |
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