PLUNC is a secreted product of neutrophil granules
Airway epithelia and neutrophils are frequently recruited to release host defense factors in response to a variety of pulmonary pathogens. One abundant product of airway epithelia is palate, lung, nasal epithelium clone (PLUNC), a proposed innate immune protein expressed in submucosal glands and sur...
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| Veröffentlicht in: | Journal of leukocyte biology 2008-05, Vol.83 (5), p.1201-1206 |
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| container_issue | 5 |
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| container_title | Journal of leukocyte biology |
| container_volume | 83 |
| creator | Bartlett, Jennifer A. Hicks, Benjamin J. Schlomann, Jamie M. Ramachandran, Shyam Nauseef, William M. McCray, Paul B. |
| description | Airway epithelia and neutrophils are frequently recruited to release host defense factors in response to a variety of pulmonary pathogens. One abundant product of airway epithelia is palate, lung, nasal epithelium clone (PLUNC), a proposed innate immune protein expressed in submucosal glands and surface airway epithelia. In this study, we report the expression of PLUNC in human neutrophils, a previously unrecognized source of this protein. Immunoblots performed on polymorphonuclear cell (PMN) lysates and PMN subcellular fractions indicated that PLUNC was present in the specific granules of the neutrophil. Furthermore, secretion assays demonstrated that PLUNC protein was released by neutrophils upon stimulation with secretogogues, including formyl methionyl leucyl phenylalanine and the calcium ionophore A23187. Although recombinant PLUNC protein failed to exhibit antibacterial activity in our studies, its storage and secretion by a professional phagocytic cell support the hypothesis that PLUNC participates in an aspect of the inflammatory response that contributes to host defense. These studies suggest that PLUNC expression is less restricted than previously believed, and highlight new avenues of research for the study of PLUNC function. |
| doi_str_mv | 10.1189/jlb.0507302 |
| format | Article |
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One abundant product of airway epithelia is palate, lung, nasal epithelium clone (PLUNC), a proposed innate immune protein expressed in submucosal glands and surface airway epithelia. In this study, we report the expression of PLUNC in human neutrophils, a previously unrecognized source of this protein. Immunoblots performed on polymorphonuclear cell (PMN) lysates and PMN subcellular fractions indicated that PLUNC was present in the specific granules of the neutrophil. Furthermore, secretion assays demonstrated that PLUNC protein was released by neutrophils upon stimulation with secretogogues, including formyl methionyl leucyl phenylalanine and the calcium ionophore A23187. Although recombinant PLUNC protein failed to exhibit antibacterial activity in our studies, its storage and secretion by a professional phagocytic cell support the hypothesis that PLUNC participates in an aspect of the inflammatory response that contributes to host defense. 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One abundant product of airway epithelia is palate, lung, nasal epithelium clone (PLUNC), a proposed innate immune protein expressed in submucosal glands and surface airway epithelia. In this study, we report the expression of PLUNC in human neutrophils, a previously unrecognized source of this protein. Immunoblots performed on polymorphonuclear cell (PMN) lysates and PMN subcellular fractions indicated that PLUNC was present in the specific granules of the neutrophil. Furthermore, secretion assays demonstrated that PLUNC protein was released by neutrophils upon stimulation with secretogogues, including formyl methionyl leucyl phenylalanine and the calcium ionophore A23187. Although recombinant PLUNC protein failed to exhibit antibacterial activity in our studies, its storage and secretion by a professional phagocytic cell support the hypothesis that PLUNC participates in an aspect of the inflammatory response that contributes to host defense. These studies suggest that PLUNC expression is less restricted than previously believed, and highlight new avenues of research for the study of PLUNC function.</description><subject>Animals</subject><subject>Biomarkers, Tumor - secretion</subject><subject>BPI</subject><subject>Cell Line</subject><subject>Cells, Cultured</subject><subject>Cytoplasmic Granules - secretion</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>endotoxin</subject><subject>Glycoproteins - genetics</subject><subject>Glycoproteins - isolation & purification</subject><subject>Glycoproteins - secretion</subject><subject>Humans</subject><subject>inflammation</subject><subject>Inflammation - physiopathology</subject><subject>innate immunity</subject><subject>LBP</subject><subject>Mammals</subject><subject>Neutrophils - secretion</subject><subject>Phosphoproteins - genetics</subject><subject>Phosphoproteins - isolation & purification</subject><subject>Phosphoproteins - secretion</subject><subject>Recombinant Proteins - metabolism</subject><subject>Respiratory Mucosa - cytology</subject><subject>Respiratory Mucosa - secretion</subject><subject>Transfection</subject><issn>0741-5400</issn><issn>1938-3673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0DtPwzAUBWALgWgpTOwoEwtKuX4lzggVT1XAQGfLSa5pkNsUu1HUf4-hldiAwbJkfff46hBySmFMqSou3105Bgk5B7ZHhrTgKuVZzvfJEHJBUykABuQohHcA4CyDQzKgignJWDEk7GU6e5okTUhMErDyuMY6Wfm27qp10tpkid3at6t545I3b5adw3BMDqxxAU9294jMbm9eJ_fp9PnuYXI1TSshFU3LSomqoGVmjM0kmlJQXtraoDW1rWkhcqswlwoqlLa08ZXHASV5hrWIh4_I-TY3rvPRYVjrRRMqdM4sse2CzgoqeCazPyEDRoH_D4IopIzwYgsr34bg0eqVbxbGbzQF_VW6jqXrXelRn-1iu3KB9Y_dtRwBbEHfONz8lqUfp9eUAf1Zdd68zfvGow4L41z8gem-7xXXUn_DTxrXmJA</recordid><startdate>20080501</startdate><enddate>20080501</enddate><creator>Bartlett, Jennifer A.</creator><creator>Hicks, Benjamin J.</creator><creator>Schlomann, Jamie M.</creator><creator>Ramachandran, Shyam</creator><creator>Nauseef, William M.</creator><creator>McCray, Paul B.</creator><general>Society for Leukocyte Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20080501</creationdate><title>PLUNC is a secreted product of neutrophil granules</title><author>Bartlett, Jennifer A. ; 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| source | Wiley-Blackwell Journals; MEDLINE; Oxford University Press Journals; Free E-Journal (出版社公開部分のみ）; Alma/SFX Local Collection |
| subjects | Animals Biomarkers, Tumor - secretion BPI Cell Line Cells, Cultured Cytoplasmic Granules - secretion Electrophoresis, Polyacrylamide Gel endotoxin Glycoproteins - genetics Glycoproteins - isolation & purification Glycoproteins - secretion Humans inflammation Inflammation - physiopathology innate immunity LBP Mammals Neutrophils - secretion Phosphoproteins - genetics Phosphoproteins - isolation & purification Phosphoproteins - secretion Recombinant Proteins - metabolism Respiratory Mucosa - cytology Respiratory Mucosa - secretion Transfection |
| title | PLUNC is a secreted product of neutrophil granules |
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