Purification of TaqI endonuclease from Thermus aquaticus

A purification procedure for the thermostable restriction enzyme TaqI was developed using high-performance ion-exchange liquid chromatography. The effects of various operating conditions on the separation behaviour of TaqI endonuclease from the cell extracts were investigated for optimisation and sc...

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Veröffentlicht in:	Journal of Chromatography A 1998-12, Vol.828 (1), p.373-381
Hauptverfasser:	Altintaş, M M, Ozer, N, Ulgen, K O
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Biological and medical sciences Cation Exchange Resins Chromatography, High Pressure Liquid - methods Chromatography, Ion Exchange - methods Deoxyribonucleases, Type II Site-Specific - isolation & purification Electrophoresis, Polyacrylamide Gel Enzymes Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Hydrolases Purification TaqI endonuclease Thermus - enzymology
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