Variety discrimination in pea (Pisum sativum L.) by molecular, biochemical and morphological markers
The distinctness, uniformity and stability (DUS) requirements involve expensive, space- and time-consuming measurements of morphological traits. Moreover, for a majority of traits, interactions between genotype and environment complicate the evaluation. Molecular markers have a potential to facilita...
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Veröffentlicht in: | Journal of applied genetics 2008-01, Vol.49 (2), p.155-166 |
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description | The distinctness, uniformity and stability (DUS) requirements involve expensive, space- and time-consuming measurements of morphological traits. Moreover, for a majority of traits, interactions between genotype and environment complicate the evaluation. Molecular markers have a potential to facilitate this procedure, increase the reliability of decisions, and substantially save the time and space needed for experiments. We chose 25 varieties of pea (
Pisum sativum
L.) from the list of recommended varieties for cultivation in the Czech Republic, and made both a standard classification by 12 morphological descriptors and a classification by biochemical-molecular markers. Two isozyme systems, 10 microsatellite loci, 2 retrotransposons for multilocus inter-retrotransposon amplified polymorphism (IRAP), and 12 retrotransposon-based insertion polymorphism (RBIP) DNA markers were analysed. The main objective of the study was to examine the potential of each method for discrimination between pea varieties. The results demonstrate a high potential and resolving power of DNA-based methods. Superior in terms of high information content and discrimination power were SSR markers, owing to high allelic variation, which was the only biochemical-molecular method allowing clear identification of all varieties. Retrotransposon markers in RBIP format proved to be the most robust and easy to score method, while multilocus IRAP produced informative fingerprint already in a single analysis. Isozyme analysis offered a fast and less expensive alternative. The results showed that molecular identification could be used to assess distinctness and complement morphological assessment, especially in cases where the time frame plays an important role. Currently developed pea marker systems might serve also for germplasm management and genetic diversity studies. |
doi_str_mv | 10.1007/BF03195609 |
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Pisum sativum
L.) from the list of recommended varieties for cultivation in the Czech Republic, and made both a standard classification by 12 morphological descriptors and a classification by biochemical-molecular markers. Two isozyme systems, 10 microsatellite loci, 2 retrotransposons for multilocus inter-retrotransposon amplified polymorphism (IRAP), and 12 retrotransposon-based insertion polymorphism (RBIP) DNA markers were analysed. The main objective of the study was to examine the potential of each method for discrimination between pea varieties. The results demonstrate a high potential and resolving power of DNA-based methods. Superior in terms of high information content and discrimination power were SSR markers, owing to high allelic variation, which was the only biochemical-molecular method allowing clear identification of all varieties. Retrotransposon markers in RBIP format proved to be the most robust and easy to score method, while multilocus IRAP produced informative fingerprint already in a single analysis. Isozyme analysis offered a fast and less expensive alternative. The results showed that molecular identification could be used to assess distinctness and complement morphological assessment, especially in cases where the time frame plays an important role. Currently developed pea marker systems might serve also for germplasm management and genetic diversity studies.</description><identifier>ISSN: 1234-1983</identifier><identifier>EISSN: 2190-3883</identifier><identifier>DOI: 10.1007/BF03195609</identifier><identifier>PMID: 18436990</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Animal Genetics and Genomics ; Biomarkers ; Biomedical and Life Sciences ; DNA, Plant - genetics ; Human Genetics ; Life Sciences ; Microbial Genetics and Genomics ; Microsatellite Repeats - genetics ; Original Article ; Pisum sativum ; Pisum sativum - genetics ; Pisum sativum - metabolism ; Plant Genetics and Genomics ; Polymorphism, Genetic ; Retroelements ; Species Specificity</subject><ispartof>Journal of applied genetics, 2008-01, Vol.49 (2), p.155-166</ispartof><rights>Institute of Plant Genetics, Polish Academy of Sciences, Poznan 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-6c1bb02710ca7fcd3763df6069ac1a1be4538ac77addace7212792828f7997613</citedby><cites>FETCH-LOGICAL-c352t-6c1bb02710ca7fcd3763df6069ac1a1be4538ac77addace7212792828f7997613</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/BF03195609$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/BF03195609$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18436990$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Smykal, Peter</creatorcontrib><creatorcontrib>Horacek, Jiri</creatorcontrib><creatorcontrib>Dostalova, Radmila</creatorcontrib><creatorcontrib>Hybl, Miroslav</creatorcontrib><title>Variety discrimination in pea (Pisum sativum L.) by molecular, biochemical and morphological markers</title><title>Journal of applied genetics</title><addtitle>J Appl Genet</addtitle><addtitle>J Appl Genet</addtitle><description>The distinctness, uniformity and stability (DUS) requirements involve expensive, space- and time-consuming measurements of morphological traits. Moreover, for a majority of traits, interactions between genotype and environment complicate the evaluation. Molecular markers have a potential to facilitate this procedure, increase the reliability of decisions, and substantially save the time and space needed for experiments. We chose 25 varieties of pea (
Pisum sativum
L.) from the list of recommended varieties for cultivation in the Czech Republic, and made both a standard classification by 12 morphological descriptors and a classification by biochemical-molecular markers. Two isozyme systems, 10 microsatellite loci, 2 retrotransposons for multilocus inter-retrotransposon amplified polymorphism (IRAP), and 12 retrotransposon-based insertion polymorphism (RBIP) DNA markers were analysed. The main objective of the study was to examine the potential of each method for discrimination between pea varieties. The results demonstrate a high potential and resolving power of DNA-based methods. Superior in terms of high information content and discrimination power were SSR markers, owing to high allelic variation, which was the only biochemical-molecular method allowing clear identification of all varieties. Retrotransposon markers in RBIP format proved to be the most robust and easy to score method, while multilocus IRAP produced informative fingerprint already in a single analysis. Isozyme analysis offered a fast and less expensive alternative. The results showed that molecular identification could be used to assess distinctness and complement morphological assessment, especially in cases where the time frame plays an important role. Currently developed pea marker systems might serve also for germplasm management and genetic diversity studies.</description><subject>Animal Genetics and Genomics</subject><subject>Biomarkers</subject><subject>Biomedical and Life Sciences</subject><subject>DNA, Plant - genetics</subject><subject>Human Genetics</subject><subject>Life Sciences</subject><subject>Microbial Genetics and Genomics</subject><subject>Microsatellite Repeats - genetics</subject><subject>Original Article</subject><subject>Pisum sativum</subject><subject>Pisum sativum - genetics</subject><subject>Pisum sativum - metabolism</subject><subject>Plant Genetics and Genomics</subject><subject>Polymorphism, Genetic</subject><subject>Retroelements</subject><subject>Species Specificity</subject><issn>1234-1983</issn><issn>2190-3883</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLAzEUhYMotlY3_gDJSnxNzWOax1KLVaGgC3U7ZDKZNnVmMiYzQv-90RbcCK4unPNx4H4AHGM0xgjx69sZolhOGJI7YEiwRAkVgu6CISY0TbAUdAAOQlghREXKyT4YYJFSJiUaguJNeWu6NSxs0N7WtlGddQ20DWyNgmfPNvQ1DDH8jHc-Pof5GtauMrqvlL-CuXV6aWqrVQVVU8TKt0tXucVPUiv_bnw4BHulqoI52t4ReJ3dvUwfkvnT_eP0Zp5oOiFdwjTOc0Q4RlrxUheUM1qUDDGpNFY4N-mECqU5V0WhtOEEEy6JIKLkUnKG6QicbnZb7z56E7qsjl-ZqlKNcX3ImMSUTwT6FyRIsJRGcSNwsQG1dyF4U2ZtlKT8OsMo-5af_cqP8Ml2tc9rU_yiW9sRuNwAIVbNwvhs5XrfRCV_zX0B1uOMew</recordid><startdate>20080101</startdate><enddate>20080101</enddate><creator>Smykal, Peter</creator><creator>Horacek, Jiri</creator><creator>Dostalova, Radmila</creator><creator>Hybl, Miroslav</creator><general>Springer-Verlag</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20080101</creationdate><title>Variety discrimination in pea (Pisum sativum L.) by molecular, biochemical and morphological markers</title><author>Smykal, Peter ; Horacek, Jiri ; Dostalova, Radmila ; Hybl, Miroslav</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-6c1bb02710ca7fcd3763df6069ac1a1be4538ac77addace7212792828f7997613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animal Genetics and Genomics</topic><topic>Biomarkers</topic><topic>Biomedical and Life Sciences</topic><topic>DNA, Plant - genetics</topic><topic>Human Genetics</topic><topic>Life Sciences</topic><topic>Microbial Genetics and Genomics</topic><topic>Microsatellite Repeats - genetics</topic><topic>Original Article</topic><topic>Pisum sativum</topic><topic>Pisum sativum - genetics</topic><topic>Pisum sativum - metabolism</topic><topic>Plant Genetics and Genomics</topic><topic>Polymorphism, Genetic</topic><topic>Retroelements</topic><topic>Species Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Smykal, Peter</creatorcontrib><creatorcontrib>Horacek, Jiri</creatorcontrib><creatorcontrib>Dostalova, Radmila</creatorcontrib><creatorcontrib>Hybl, Miroslav</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Smykal, Peter</au><au>Horacek, Jiri</au><au>Dostalova, Radmila</au><au>Hybl, Miroslav</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Variety discrimination in pea (Pisum sativum L.) by molecular, biochemical and morphological markers</atitle><jtitle>Journal of applied genetics</jtitle><stitle>J Appl Genet</stitle><addtitle>J Appl Genet</addtitle><date>2008-01-01</date><risdate>2008</risdate><volume>49</volume><issue>2</issue><spage>155</spage><epage>166</epage><pages>155-166</pages><issn>1234-1983</issn><eissn>2190-3883</eissn><abstract>The distinctness, uniformity and stability (DUS) requirements involve expensive, space- and time-consuming measurements of morphological traits. Moreover, for a majority of traits, interactions between genotype and environment complicate the evaluation. Molecular markers have a potential to facilitate this procedure, increase the reliability of decisions, and substantially save the time and space needed for experiments. We chose 25 varieties of pea (
Pisum sativum
L.) from the list of recommended varieties for cultivation in the Czech Republic, and made both a standard classification by 12 morphological descriptors and a classification by biochemical-molecular markers. Two isozyme systems, 10 microsatellite loci, 2 retrotransposons for multilocus inter-retrotransposon amplified polymorphism (IRAP), and 12 retrotransposon-based insertion polymorphism (RBIP) DNA markers were analysed. The main objective of the study was to examine the potential of each method for discrimination between pea varieties. The results demonstrate a high potential and resolving power of DNA-based methods. Superior in terms of high information content and discrimination power were SSR markers, owing to high allelic variation, which was the only biochemical-molecular method allowing clear identification of all varieties. Retrotransposon markers in RBIP format proved to be the most robust and easy to score method, while multilocus IRAP produced informative fingerprint already in a single analysis. Isozyme analysis offered a fast and less expensive alternative. The results showed that molecular identification could be used to assess distinctness and complement morphological assessment, especially in cases where the time frame plays an important role. Currently developed pea marker systems might serve also for germplasm management and genetic diversity studies.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>18436990</pmid><doi>10.1007/BF03195609</doi><tpages>12</tpages></addata></record> |
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subjects | Animal Genetics and Genomics Biomarkers Biomedical and Life Sciences DNA, Plant - genetics Human Genetics Life Sciences Microbial Genetics and Genomics Microsatellite Repeats - genetics Original Article Pisum sativum Pisum sativum - genetics Pisum sativum - metabolism Plant Genetics and Genomics Polymorphism, Genetic Retroelements Species Specificity |
title | Variety discrimination in pea (Pisum sativum L.) by molecular, biochemical and morphological markers |
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