RNA Quantitation by Fluorescence-Based Solution Assay: RiboGreen Reagent Characterization

We describe the development of a sensitive fluorescence-based solution assay for RNA using a new dye, RiboGreen RNA quantitation reagent. RiboGreen reagent exhibits >1000-fold fluorescence enhancement and high quantum yield (0.65) upon binding nucleic acids, with excitation and emission maxima ne...

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Veröffentlicht in:	Analytical biochemistry 1998-12, Vol.265 (2), p.368-374
Hauptverfasser:	Jones, Laurie J., Yue, Stephen T., Cheung, Ching-Ying, Singer, Victoria L.
Format:	Artikel
Sprache:	eng
Schlagworte:	ethidium bromide fluorescence Fluorescent Dyes - chemistry Indicators and Reagents - chemistry nucleic acid stain RiboGreen RNA - analysis RNA detection RNA quantitation Sensitivity and Specificity Spectrometry, Fluorescence
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