Applicability of SSCP analysis for MHC genotyping: fingerprinting of Ovar-DRB1 exon 2 alleles from Finnish and Russian breeds

The applicability of single strand conformation polymorphism (SSCP) analysis for major histocompatibility complex (MHC) genotyping in sheep was studied. A panel of Ovar‐DRB1 exon 2 ‘allele fingerprints’ was defined. The panel could accelerate DRB1 genotyping of new breeds when already existing seque...

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Veröffentlicht in:	Animal genetics 1998-12, Vol.29 (6), p.453-455
Hauptverfasser:	Kostia, S., Kantanen, J., Kolkkala, M., Varvio, S-L.
Format:	Artikel
Sprache:	eng
Schlagworte:	allele fingerprinting Alleles Animals Biological and medical sciences Classical genetics, quantitative genetics, hybrids DNA Fingerprinting - veterinary Exons Fundamental and applied biological sciences. Psychology Genetic Variation Genetics of eukaryotes. Biological and molecular evolution Genotype genotyping Histocompatibility Antigens - genetics Major Histocompatibility Complex - genetics MhcOvar Polymorphism, Single-Stranded Conformational Sheep - genetics SSCP Vertebrata
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Beschreibung
Zusammenfassung:	The applicability of single strand conformation polymorphism (SSCP) analysis for major histocompatibility complex (MHC) genotyping in sheep was studied. A panel of Ovar‐DRB1 exon 2 ‘allele fingerprints’ was defined. The panel could accelerate DRB1 genotyping of new breeds when already existing sequences are used as references in SSCP analysis. In this study, seven new exon 2 sequences and 19 different alleles in total were detected from 31 animals of Finnish and Russian sheep breeds. Ovar‐DRB1*0201 was detected in all the six grey Finnsheep animals included in this study, suggesting reduced MHC diversity within these animals.
ISSN:	0268-9146 1365-2052
DOI:	10.1046/j.1365-2052.1998.296376.x