Metabolic studies on eel ( Anguilla anguilla L.) hepatocytes in primary culture: effect of 17 β-estradiol and growth hormone
Previous studies demonstrated that native and recombinant growth hormone from mammalian and fish species potentiate the estrogenic induction of vitellogenin synthesis by cultured eel hepatocytes. In the present study, the metabolic competence (respiratory activity and estradiol catabolism) of cultur...
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| Veröffentlicht in: | Comparative biochemistry and physiology. Part A, Molecular & integrative physiology Molecular & integrative physiology, 1998-09, Vol.121 (1), p.35-44 |
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| container_title | Comparative biochemistry and physiology. Part A, Molecular & integrative physiology |
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| creator | Peyon, Pierre Calvayrac, Régis Baloche, Sylvie Burzawa-Gérard, Elisabeth |
| description | Previous studies demonstrated that native and recombinant growth hormone from mammalian and fish species potentiate the estrogenic induction of vitellogenin synthesis by cultured eel hepatocytes. In the present study, the metabolic competence (respiratory activity and estradiol catabolism) of cultured hepatocytes and their functional capacity to synthesize a specific protein, vitellogenin, in the presence of estradiol and/or bovine growth hormone was investigated. In addition, we examined the possible role of insulin-like growth factors as mediators of growth hormone. Hepatocytes retain a high level of metabolic activity under the primary culture conditions applied. Estradiol has a half life of several hours in the hepatocyte culture, and is metabolized into conjugated forms. Estradiol and/or growth hormone had no effects on respiratory activity of the cultured hepatocytes. Moreover, the estradiol catabolic parameters were not affected by growth hormone. Finally, human and trout recombinant insulin-like growth factors do not potentiate vitellogenin synthesis induced by estradiol. |
| doi_str_mv | 10.1016/S1095-6433(98)10099-5 |
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In the present study, the metabolic competence (respiratory activity and estradiol catabolism) of cultured hepatocytes and their functional capacity to synthesize a specific protein, vitellogenin, in the presence of estradiol and/or bovine growth hormone was investigated. In addition, we examined the possible role of insulin-like growth factors as mediators of growth hormone. Hepatocytes retain a high level of metabolic activity under the primary culture conditions applied. Estradiol has a half life of several hours in the hepatocyte culture, and is metabolized into conjugated forms. Estradiol and/or growth hormone had no effects on respiratory activity of the cultured hepatocytes. Moreover, the estradiol catabolic parameters were not affected by growth hormone. Finally, human and trout recombinant insulin-like growth factors do not potentiate vitellogenin synthesis induced by estradiol.</description><identifier>ISSN: 1095-6433</identifier><identifier>EISSN: 1531-4332</identifier><identifier>DOI: 10.1016/S1095-6433(98)10099-5</identifier><identifier>PMID: 9883567</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Anguilla ; Anguilla anguilla ; Animals ; Brackish ; Catabolism ; Catalase ; Catalase - metabolism ; Cells, Cultured ; Conjugation ; DNA - metabolism ; Estradiol - pharmacology ; Female ; Freshwater ; Hormone ; Human Growth Hormone - pharmacology ; Insulin-Like Growth Factor I - metabolism ; Insulin-Like Growth Factor II - metabolism ; Insulin-like growth factors ; Liver ; Liver - cytology ; Liver - enzymology ; Marine ; Microelectrodes ; Oxygen consumption ; Oxygen Consumption - drug effects ; Steroid ; Vitellogenin ; Vitellogenins - metabolism ; Vitellogenins - secretion</subject><ispartof>Comparative biochemistry and physiology. 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Part A, Molecular & integrative physiology</title><addtitle>Comp Biochem Physiol A Mol Integr Physiol</addtitle><description>Previous studies demonstrated that native and recombinant growth hormone from mammalian and fish species potentiate the estrogenic induction of vitellogenin synthesis by cultured eel hepatocytes. In the present study, the metabolic competence (respiratory activity and estradiol catabolism) of cultured hepatocytes and their functional capacity to synthesize a specific protein, vitellogenin, in the presence of estradiol and/or bovine growth hormone was investigated. In addition, we examined the possible role of insulin-like growth factors as mediators of growth hormone. Hepatocytes retain a high level of metabolic activity under the primary culture conditions applied. Estradiol has a half life of several hours in the hepatocyte culture, and is metabolized into conjugated forms. Estradiol and/or growth hormone had no effects on respiratory activity of the cultured hepatocytes. Moreover, the estradiol catabolic parameters were not affected by growth hormone. Finally, human and trout recombinant insulin-like growth factors do not potentiate vitellogenin synthesis induced by estradiol.</description><subject>Anguilla</subject><subject>Anguilla anguilla</subject><subject>Animals</subject><subject>Brackish</subject><subject>Catabolism</subject><subject>Catalase</subject><subject>Catalase - metabolism</subject><subject>Cells, Cultured</subject><subject>Conjugation</subject><subject>DNA - metabolism</subject><subject>Estradiol - pharmacology</subject><subject>Female</subject><subject>Freshwater</subject><subject>Hormone</subject><subject>Human Growth Hormone - pharmacology</subject><subject>Insulin-Like Growth Factor I - metabolism</subject><subject>Insulin-Like Growth Factor II - metabolism</subject><subject>Insulin-like growth factors</subject><subject>Liver</subject><subject>Liver - cytology</subject><subject>Liver - enzymology</subject><subject>Marine</subject><subject>Microelectrodes</subject><subject>Oxygen consumption</subject><subject>Oxygen Consumption - drug effects</subject><subject>Steroid</subject><subject>Vitellogenin</subject><subject>Vitellogenins - metabolism</subject><subject>Vitellogenins - secretion</subject><issn>1095-6433</issn><issn>1531-4332</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9u1DAQxi0EKqXwCJV8Qu0hrceOE5sLqqryR1rEAThbjj3pGmXjxXZAPfSleBCeCbe77bW-eDTzmxn7-wg5BnYGDLrzb8C0bLpWiBOtToExrRv5jByCFNDULH9e4wfkJXmV809WTwvtATnQSgnZ9Yfk9gsWO8QpOJrL4gNmGmeKONETejFfL2GaLLUPwerslK5xa0t0N6WiYabbFDY23VC3TGVJ-I7iOKIrNI4Uevrvb4O5JOtDnOoYT69T_FPWdB3TJs74mrwY7ZTxzf4-Ij8-XH2__NSsvn78fHmxapzQUBqUDtpu1Fqj65hyg-4G1zP0XHBuUfeMA_PKy0FyQATWK41D77kTtlfCiyPydjd3m-Kvpb7IbEJ2WL80Y1yy6TQI3rH2SRD6VjDNVQXlDnQp5pxwNHshDDBz54-598fciW-0Mvf-GFn7jvcLlmGD_rFrb0itv9_VscrxO2Ay2QWcHfqQqqzGx_DEhv_TqaCG</recordid><startdate>19980901</startdate><enddate>19980901</enddate><creator>Peyon, Pierre</creator><creator>Calvayrac, Régis</creator><creator>Baloche, Sylvie</creator><creator>Burzawa-Gérard, Elisabeth</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TN</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>19980901</creationdate><title>Metabolic studies on eel ( Anguilla anguilla L.) hepatocytes in primary culture: effect of 17 β-estradiol and growth hormone</title><author>Peyon, Pierre ; Calvayrac, Régis ; Baloche, Sylvie ; Burzawa-Gérard, Elisabeth</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-e5c146f999ec608cb96bc70ed2322ae970210d8d5b521ee10789eb7d2c3a783d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Anguilla</topic><topic>Anguilla anguilla</topic><topic>Animals</topic><topic>Brackish</topic><topic>Catabolism</topic><topic>Catalase</topic><topic>Catalase - metabolism</topic><topic>Cells, Cultured</topic><topic>Conjugation</topic><topic>DNA - metabolism</topic><topic>Estradiol - pharmacology</topic><topic>Female</topic><topic>Freshwater</topic><topic>Hormone</topic><topic>Human Growth Hormone - pharmacology</topic><topic>Insulin-Like Growth Factor I - metabolism</topic><topic>Insulin-Like Growth Factor II - metabolism</topic><topic>Insulin-like growth factors</topic><topic>Liver</topic><topic>Liver - cytology</topic><topic>Liver - enzymology</topic><topic>Marine</topic><topic>Microelectrodes</topic><topic>Oxygen consumption</topic><topic>Oxygen Consumption - drug effects</topic><topic>Steroid</topic><topic>Vitellogenin</topic><topic>Vitellogenins - metabolism</topic><topic>Vitellogenins - secretion</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Peyon, Pierre</creatorcontrib><creatorcontrib>Calvayrac, Régis</creatorcontrib><creatorcontrib>Baloche, Sylvie</creatorcontrib><creatorcontrib>Burzawa-Gérard, Elisabeth</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oceanic Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Comparative biochemistry and physiology. 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Part A, Molecular & integrative physiology</jtitle><addtitle>Comp Biochem Physiol A Mol Integr Physiol</addtitle><date>1998-09-01</date><risdate>1998</risdate><volume>121</volume><issue>1</issue><spage>35</spage><epage>44</epage><pages>35-44</pages><issn>1095-6433</issn><eissn>1531-4332</eissn><abstract>Previous studies demonstrated that native and recombinant growth hormone from mammalian and fish species potentiate the estrogenic induction of vitellogenin synthesis by cultured eel hepatocytes. In the present study, the metabolic competence (respiratory activity and estradiol catabolism) of cultured hepatocytes and their functional capacity to synthesize a specific protein, vitellogenin, in the presence of estradiol and/or bovine growth hormone was investigated. In addition, we examined the possible role of insulin-like growth factors as mediators of growth hormone. Hepatocytes retain a high level of metabolic activity under the primary culture conditions applied. Estradiol has a half life of several hours in the hepatocyte culture, and is metabolized into conjugated forms. Estradiol and/or growth hormone had no effects on respiratory activity of the cultured hepatocytes. Moreover, the estradiol catabolic parameters were not affected by growth hormone. Finally, human and trout recombinant insulin-like growth factors do not potentiate vitellogenin synthesis induced by estradiol.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>9883567</pmid><doi>10.1016/S1095-6433(98)10099-5</doi><tpages>10</tpages></addata></record> |
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| ispartof | Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 1998-09, Vol.121 (1), p.35-44 |
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| subjects | Anguilla Anguilla anguilla Animals Brackish Catabolism Catalase Catalase - metabolism Cells, Cultured Conjugation DNA - metabolism Estradiol - pharmacology Female Freshwater Hormone Human Growth Hormone - pharmacology Insulin-Like Growth Factor I - metabolism Insulin-Like Growth Factor II - metabolism Insulin-like growth factors Liver Liver - cytology Liver - enzymology Marine Microelectrodes Oxygen consumption Oxygen Consumption - drug effects Steroid Vitellogenin Vitellogenins - metabolism Vitellogenins - secretion |
| title | Metabolic studies on eel ( Anguilla anguilla L.) hepatocytes in primary culture: effect of 17 β-estradiol and growth hormone |
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