N-(4-nitro-2,1,3-benzoxadiazoyl-7-yl)- N-methyl-2-aminoacetohydrazide (NBD-CO-Hz) as a precolumn fluorescent derivatization reagent for carboxylic acids in high-performance liquid chromatography
A new fluorescent reagent for carboxylic acids, N-(4-nitro-2,1,3-benzoxadiazoyl-7-yl)- N-methyl-2-aminoacetohydrazide (NBD-CO-Hz) was synthesized and its applicability as a precolumn derivatization reagent in high-performance liquid chromatography was examined. NBD-CO-Hz reacted with 2-arylpropionic...
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| Veröffentlicht in: | Journal of pharmaceutical and biomedical analysis 1998-09, Vol.17 (6), p.1065-1070 |
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| container_issue | 6 |
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| container_title | Journal of pharmaceutical and biomedical analysis |
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| creator | Santa, T Takeda, A Uchiyama, S Fukushima, T Homma, H Suzuki, S Yokosu, H Lim, C.K Imai, K |
| description | A new fluorescent reagent for carboxylic acids,
N-(4-nitro-2,1,3-benzoxadiazoyl-7-yl)-
N-methyl-2-aminoacetohydrazide (NBD-CO-Hz) was synthesized and its applicability as a precolumn derivatization reagent in high-performance liquid chromatography was examined. NBD-CO-Hz reacted with 2-arylpropionic acids (2-APAs), a group of non-steroidal antiinflammatory drugs (NSAIDs) in the presence of a condensing agent, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and pyridine at room temperature for 2 h to give fluorescent adducts. The reaction solution was subjected to a reversed phase or a chiral stationary phase HPLC and the derivatives were detected fluorometrically at a wavelength of 530 nm with an exitation of 475 nm. The detection limits were in the fmol range on column. |
| doi_str_mv | 10.1016/S0731-7085(98)00072-7 |
| format | Article |
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N-(4-nitro-2,1,3-benzoxadiazoyl-7-yl)-
N-methyl-2-aminoacetohydrazide (NBD-CO-Hz) was synthesized and its applicability as a precolumn derivatization reagent in high-performance liquid chromatography was examined. NBD-CO-Hz reacted with 2-arylpropionic acids (2-APAs), a group of non-steroidal antiinflammatory drugs (NSAIDs) in the presence of a condensing agent, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and pyridine at room temperature for 2 h to give fluorescent adducts. The reaction solution was subjected to a reversed phase or a chiral stationary phase HPLC and the derivatives were detected fluorometrically at a wavelength of 530 nm with an exitation of 475 nm. The detection limits were in the fmol range on column.</description><identifier>ISSN: 0731-7085</identifier><identifier>EISSN: 1873-264X</identifier><identifier>DOI: 10.1016/S0731-7085(98)00072-7</identifier><identifier>PMID: 9884196</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>2-Arylpropionic acid ; Anti-Inflammatory Agents, Non-Steroidal - chemistry ; Carboxylic Acids - analysis ; Chiral stationary phase ; Chromatography, High Pressure Liquid - methods ; Enantiomeric separation ; Fluorescence ; Fluorescence derivatization ; HPLC ; Hydrazines - chemical synthesis ; Hydrazines - chemistry ; N-(4-nitro-2,1,3-benzoxadiazoyl-7-yl)- N-methyl-2-aminoacetohydrazide (NBD-CO-Hz) ; Oxadiazoles - chemical synthesis ; Oxadiazoles - chemistry ; Propionates - chemistry ; Pyridines - chemistry ; Stereoisomerism</subject><ispartof>Journal of pharmaceutical and biomedical analysis, 1998-09, Vol.17 (6), p.1065-1070</ispartof><rights>1998 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c360t-7315f4439666c0d1ab93d052bf4284b9eb4d598993eb15c8dc7b88c0803e17e33</citedby><cites>FETCH-LOGICAL-c360t-7315f4439666c0d1ab93d052bf4284b9eb4d598993eb15c8dc7b88c0803e17e33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0731-7085(98)00072-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27911,27912,45982</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9884196$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Santa, T</creatorcontrib><creatorcontrib>Takeda, A</creatorcontrib><creatorcontrib>Uchiyama, S</creatorcontrib><creatorcontrib>Fukushima, T</creatorcontrib><creatorcontrib>Homma, H</creatorcontrib><creatorcontrib>Suzuki, S</creatorcontrib><creatorcontrib>Yokosu, H</creatorcontrib><creatorcontrib>Lim, C.K</creatorcontrib><creatorcontrib>Imai, K</creatorcontrib><title>N-(4-nitro-2,1,3-benzoxadiazoyl-7-yl)- N-methyl-2-aminoacetohydrazide (NBD-CO-Hz) as a precolumn fluorescent derivatization reagent for carboxylic acids in high-performance liquid chromatography</title><title>Journal of pharmaceutical and biomedical analysis</title><addtitle>J Pharm Biomed Anal</addtitle><description>A new fluorescent reagent for carboxylic acids,
N-(4-nitro-2,1,3-benzoxadiazoyl-7-yl)-
N-methyl-2-aminoacetohydrazide (NBD-CO-Hz) was synthesized and its applicability as a precolumn derivatization reagent in high-performance liquid chromatography was examined. NBD-CO-Hz reacted with 2-arylpropionic acids (2-APAs), a group of non-steroidal antiinflammatory drugs (NSAIDs) in the presence of a condensing agent, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and pyridine at room temperature for 2 h to give fluorescent adducts. The reaction solution was subjected to a reversed phase or a chiral stationary phase HPLC and the derivatives were detected fluorometrically at a wavelength of 530 nm with an exitation of 475 nm. The detection limits were in the fmol range on column.</description><subject>2-Arylpropionic acid</subject><subject>Anti-Inflammatory Agents, Non-Steroidal - chemistry</subject><subject>Carboxylic Acids - analysis</subject><subject>Chiral stationary phase</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Enantiomeric separation</subject><subject>Fluorescence</subject><subject>Fluorescence derivatization</subject><subject>HPLC</subject><subject>Hydrazines - chemical synthesis</subject><subject>Hydrazines - chemistry</subject><subject>N-(4-nitro-2,1,3-benzoxadiazoyl-7-yl)- N-methyl-2-aminoacetohydrazide (NBD-CO-Hz)</subject><subject>Oxadiazoles - chemical synthesis</subject><subject>Oxadiazoles - chemistry</subject><subject>Propionates - chemistry</subject><subject>Pyridines - chemistry</subject><subject>Stereoisomerism</subject><issn>0731-7085</issn><issn>1873-264X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAUhSMEKkPhESp5hWakGuw4P_YKwVAoUjVdABI7y7FvJkZJnNpJ1eTx-mR4OqNuWViWfc69V_d8SXJByQdKaPHxJykZxSXh-VrwDSGkTHH5IllRXjKcFtmfl8nq2fI6eRPC32jKqcjOkjPBeUZFsUoed3id4d6O3uH0kl4yXEG_uAdlrFrc3OISz-0Gox3uYGziO8Wqs71TGkbXzMarxRpA692Xr3h7i6-XDVIBKTR40K6duh7V7eQ8BA39iAx4e69Gu8TjeuRB7Q_ftfNIK1-5h7m1GiltTUC2R43dN3gAH_VO9RpQa-8ma5BuvOvU6PZeDc38NnlVqzbAu9N9nvz-dvVre41vbr__2H6-wZoVZMQxirzOMiaKotDEUFUJZkieVnWW8qwSUGUmF1wIBhXNNTe6rDjXhBMGtATGzpP3x76Dd3cThFF2Nq7VtqoHNwVZCMpoRvJozI9G7V0IHmo5eNspP0tK5IGdfGInD2Ck4PKJnSxj3cVpwFR1YJ6rTrCi_umoQ9zy3oKXQVuIuRgb0x6lcfY_E_4Bj_urUA</recordid><startdate>19980901</startdate><enddate>19980901</enddate><creator>Santa, T</creator><creator>Takeda, A</creator><creator>Uchiyama, S</creator><creator>Fukushima, T</creator><creator>Homma, H</creator><creator>Suzuki, S</creator><creator>Yokosu, H</creator><creator>Lim, C.K</creator><creator>Imai, K</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19980901</creationdate><title>N-(4-nitro-2,1,3-benzoxadiazoyl-7-yl)- N-methyl-2-aminoacetohydrazide (NBD-CO-Hz) as a precolumn fluorescent derivatization reagent for carboxylic acids in high-performance liquid chromatography</title><author>Santa, T ; Takeda, A ; Uchiyama, S ; Fukushima, T ; Homma, H ; Suzuki, S ; Yokosu, H ; Lim, C.K ; Imai, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-7315f4439666c0d1ab93d052bf4284b9eb4d598993eb15c8dc7b88c0803e17e33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>2-Arylpropionic acid</topic><topic>Anti-Inflammatory Agents, Non-Steroidal - chemistry</topic><topic>Carboxylic Acids - analysis</topic><topic>Chiral stationary phase</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Enantiomeric separation</topic><topic>Fluorescence</topic><topic>Fluorescence derivatization</topic><topic>HPLC</topic><topic>Hydrazines - chemical synthesis</topic><topic>Hydrazines - chemistry</topic><topic>N-(4-nitro-2,1,3-benzoxadiazoyl-7-yl)- N-methyl-2-aminoacetohydrazide (NBD-CO-Hz)</topic><topic>Oxadiazoles - chemical synthesis</topic><topic>Oxadiazoles - chemistry</topic><topic>Propionates - chemistry</topic><topic>Pyridines - chemistry</topic><topic>Stereoisomerism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Santa, T</creatorcontrib><creatorcontrib>Takeda, A</creatorcontrib><creatorcontrib>Uchiyama, S</creatorcontrib><creatorcontrib>Fukushima, T</creatorcontrib><creatorcontrib>Homma, H</creatorcontrib><creatorcontrib>Suzuki, S</creatorcontrib><creatorcontrib>Yokosu, H</creatorcontrib><creatorcontrib>Lim, C.K</creatorcontrib><creatorcontrib>Imai, K</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Santa, T</au><au>Takeda, A</au><au>Uchiyama, S</au><au>Fukushima, T</au><au>Homma, H</au><au>Suzuki, S</au><au>Yokosu, H</au><au>Lim, C.K</au><au>Imai, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>N-(4-nitro-2,1,3-benzoxadiazoyl-7-yl)- N-methyl-2-aminoacetohydrazide (NBD-CO-Hz) as a precolumn fluorescent derivatization reagent for carboxylic acids in high-performance liquid chromatography</atitle><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle><addtitle>J Pharm Biomed Anal</addtitle><date>1998-09-01</date><risdate>1998</risdate><volume>17</volume><issue>6</issue><spage>1065</spage><epage>1070</epage><pages>1065-1070</pages><issn>0731-7085</issn><eissn>1873-264X</eissn><abstract>A new fluorescent reagent for carboxylic acids,
N-(4-nitro-2,1,3-benzoxadiazoyl-7-yl)-
N-methyl-2-aminoacetohydrazide (NBD-CO-Hz) was synthesized and its applicability as a precolumn derivatization reagent in high-performance liquid chromatography was examined. NBD-CO-Hz reacted with 2-arylpropionic acids (2-APAs), a group of non-steroidal antiinflammatory drugs (NSAIDs) in the presence of a condensing agent, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and pyridine at room temperature for 2 h to give fluorescent adducts. The reaction solution was subjected to a reversed phase or a chiral stationary phase HPLC and the derivatives were detected fluorometrically at a wavelength of 530 nm with an exitation of 475 nm. The detection limits were in the fmol range on column.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>9884196</pmid><doi>10.1016/S0731-7085(98)00072-7</doi><tpages>6</tpages></addata></record> |
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| ispartof | Journal of pharmaceutical and biomedical analysis, 1998-09, Vol.17 (6), p.1065-1070 |
| issn | 0731-7085 1873-264X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69131405 |
| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | 2-Arylpropionic acid Anti-Inflammatory Agents, Non-Steroidal - chemistry Carboxylic Acids - analysis Chiral stationary phase Chromatography, High Pressure Liquid - methods Enantiomeric separation Fluorescence Fluorescence derivatization HPLC Hydrazines - chemical synthesis Hydrazines - chemistry N-(4-nitro-2,1,3-benzoxadiazoyl-7-yl)- N-methyl-2-aminoacetohydrazide (NBD-CO-Hz) Oxadiazoles - chemical synthesis Oxadiazoles - chemistry Propionates - chemistry Pyridines - chemistry Stereoisomerism |
| title | N-(4-nitro-2,1,3-benzoxadiazoyl-7-yl)- N-methyl-2-aminoacetohydrazide (NBD-CO-Hz) as a precolumn fluorescent derivatization reagent for carboxylic acids in high-performance liquid chromatography |
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