Cartilage formation in a hollow fiber bioreactor studied by proton magnetic resonance microscopy
The ideal in vitro system for investigating the regulation of cartilage formation and maintenance would allow for three-dimensional tissue growth, a wide range of biochemical interventions, and non-destructive evaluation. We have developed a hollow fiber bioreactor (HFBR) system which meets these cr...
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| Veröffentlicht in: | Matrix biology 1998-11, Vol.17 (7), p.513-523 |
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| container_title | Matrix biology |
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| creator | Potter, K. Butler, J.J. Adams, C. Fishbein, K.W. McFarland, E.W. Horton, W.E. Spencer, R.G.S. |
| description | The ideal
in vitro system for investigating the regulation of cartilage formation and maintenance would allow for three-dimensional tissue growth, a wide range of biochemical interventions, and non-destructive evaluation. We have developed a hollow fiber bioreactor (HFBR) system which meets these criteria. After injection with embryonic chick sternal chondrocytes, neocartilage is elaborated around the hollow fibers, reaching a thickness of up to a millimeter after four weeks of growth. This process was monitored over time with nuclear magnetic resonance (NMR) microimaging and correlative biochemical and histologic analyses. Tissue volume and cellularity increased greatly during development. This was accompanied by changes in magnetic resonance properties consistent with increased macromolecular content. Further, tissue heterogeneity, observed as regional variations in cell size in histologic sections, was also observed in quantitative NMR images. |
| doi_str_mv | 10.1016/S0945-053X(98)90099-3 |
| format | Article |
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in vitro system for investigating the regulation of cartilage formation and maintenance would allow for three-dimensional tissue growth, a wide range of biochemical interventions, and non-destructive evaluation. We have developed a hollow fiber bioreactor (HFBR) system which meets these criteria. After injection with embryonic chick sternal chondrocytes, neocartilage is elaborated around the hollow fibers, reaching a thickness of up to a millimeter after four weeks of growth. This process was monitored over time with nuclear magnetic resonance (NMR) microimaging and correlative biochemical and histologic analyses. Tissue volume and cellularity increased greatly during development. This was accompanied by changes in magnetic resonance properties consistent with increased macromolecular content. Further, tissue heterogeneity, observed as regional variations in cell size in histologic sections, was also observed in quantitative NMR images.</description><subject>Animals</subject><subject>bioreactor</subject><subject>Cartilage - chemistry</subject><subject>Cartilage - cytology</subject><subject>Cartilage - growth & development</subject><subject>cartilage formation</subject><subject>Chick Embryo</subject><subject>chondrocytes</subject><subject>Collagen - genetics</subject><subject>Culture Techniques - instrumentation</subject><subject>Culture Techniques - methods</subject><subject>Extracellular Matrix</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>nuclear magnetic resonance</subject><subject>RNA, Messenger - analysis</subject><issn>0945-053X</issn><issn>1569-1802</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtPwzAQhC0EglL4CUg-ITgE_Egc-4RQxUtC4gBI3IztbMAoiYvtgvrvSWnFldMeZmZn90PoiJIzSqg4fySqrApS8ZcTJU8VIUoVfAtNaCVUQSVh22jyZ9lD-yl9EELKspa7aFdJSQXhE_Q6MzH7zrwBbkPsTfZhwH7ABr-HrgvfuPUWIrY-RDAuh4hTXjQeGmyXeB5DHu29eRsge4cjpDCYwQHuvYshuTBfHqCd1nQJDjdzip6vr55mt8X9w83d7PK-cCUTuWDMcqhF5WhFbEuJMcpyLlnJgIJsJJW0FUpZq4wgVd02bS2AccUYVbLkkk_R8XrveNTnAlLWvU8Ous4MEBZJC0VZXdNyNFZr4-rCFKHV8-h7E5eaEr0iq3_J6hU2raT-Jav5mDvaFCxsD81faoNy1C_WOoxffnmIOjkPI4zGR3BZN8H_0_ADjHSJmA</recordid><startdate>19981101</startdate><enddate>19981101</enddate><creator>Potter, K.</creator><creator>Butler, J.J.</creator><creator>Adams, C.</creator><creator>Fishbein, K.W.</creator><creator>McFarland, E.W.</creator><creator>Horton, W.E.</creator><creator>Spencer, R.G.S.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19981101</creationdate><title>Cartilage formation in a hollow fiber bioreactor studied by proton magnetic resonance microscopy</title><author>Potter, K. ; Butler, J.J. ; Adams, C. ; Fishbein, K.W. ; McFarland, E.W. ; Horton, W.E. ; Spencer, R.G.S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c426t-22b3e765c150bf10aa9b338242e1e8d8181f699bb9a6057fdf76e239221984383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>bioreactor</topic><topic>Cartilage - chemistry</topic><topic>Cartilage - cytology</topic><topic>Cartilage - growth & development</topic><topic>cartilage formation</topic><topic>Chick Embryo</topic><topic>chondrocytes</topic><topic>Collagen - genetics</topic><topic>Culture Techniques - instrumentation</topic><topic>Culture Techniques - methods</topic><topic>Extracellular Matrix</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>nuclear magnetic resonance</topic><topic>RNA, Messenger - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Potter, K.</creatorcontrib><creatorcontrib>Butler, J.J.</creatorcontrib><creatorcontrib>Adams, C.</creatorcontrib><creatorcontrib>Fishbein, K.W.</creatorcontrib><creatorcontrib>McFarland, E.W.</creatorcontrib><creatorcontrib>Horton, W.E.</creatorcontrib><creatorcontrib>Spencer, R.G.S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Matrix biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Potter, K.</au><au>Butler, J.J.</au><au>Adams, C.</au><au>Fishbein, K.W.</au><au>McFarland, E.W.</au><au>Horton, W.E.</au><au>Spencer, R.G.S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cartilage formation in a hollow fiber bioreactor studied by proton magnetic resonance microscopy</atitle><jtitle>Matrix biology</jtitle><addtitle>Matrix Biol</addtitle><date>1998-11-01</date><risdate>1998</risdate><volume>17</volume><issue>7</issue><spage>513</spage><epage>523</epage><pages>513-523</pages><issn>0945-053X</issn><eissn>1569-1802</eissn><abstract>The ideal
in vitro system for investigating the regulation of cartilage formation and maintenance would allow for three-dimensional tissue growth, a wide range of biochemical interventions, and non-destructive evaluation. We have developed a hollow fiber bioreactor (HFBR) system which meets these criteria. After injection with embryonic chick sternal chondrocytes, neocartilage is elaborated around the hollow fibers, reaching a thickness of up to a millimeter after four weeks of growth. This process was monitored over time with nuclear magnetic resonance (NMR) microimaging and correlative biochemical and histologic analyses. Tissue volume and cellularity increased greatly during development. This was accompanied by changes in magnetic resonance properties consistent with increased macromolecular content. Further, tissue heterogeneity, observed as regional variations in cell size in histologic sections, was also observed in quantitative NMR images.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>9881603</pmid><doi>10.1016/S0945-053X(98)90099-3</doi><tpages>11</tpages></addata></record> |
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| ispartof | Matrix biology, 1998-11, Vol.17 (7), p.513-523 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals bioreactor Cartilage - chemistry Cartilage - cytology Cartilage - growth & development cartilage formation Chick Embryo chondrocytes Collagen - genetics Culture Techniques - instrumentation Culture Techniques - methods Extracellular Matrix Magnetic Resonance Spectroscopy nuclear magnetic resonance RNA, Messenger - analysis |
| title | Cartilage formation in a hollow fiber bioreactor studied by proton magnetic resonance microscopy |
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