β-Thujaplicin Zinc Chelate Induces Apoptosis in Mouse High Metastatic Melanoma B16BL6 Cells
The cytotoxic effects of β-thujaplicin and five kinds of metal chelates were examined on mouse melanoma B16BL6 cells by cell viability and lactate dehydrogenase (LDH) release assay. β-Thujaplicin-zinc chalate and β-thujaplicin-copper chelate had higher cytotoxic effects than β-thujaplicin, and the 5...
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Veröffentlicht in: | Biological & pharmaceutical bulletin 1998/12/15, Vol.21(12), pp.1258-1262 |
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creator | MIYAMOTO, Daisei ENDO, Naoko OKU, Naoto ARIMA, Yaeno SUZUKI, Takashi SUZUKI, Yasuo |
description | The cytotoxic effects of β-thujaplicin and five kinds of metal chelates were examined on mouse melanoma B16BL6 cells by cell viability and lactate dehydrogenase (LDH) release assay. β-Thujaplicin-zinc chalate and β-thujaplicin-copper chelate had higher cytotoxic effects than β-thujaplicin, and the 50% effective does (ED50) of these metal chelates were 12.5 and 25 μM, respectively. In addition, the zinc chelate induced DNA ladder formation in B16BL6 cells, as shown by the DNA fragmentation assay, suggesting that cell death induced by the zinc chelate is apoptosis. The zinc chelate also had a cytotoxic effect and induced DNA fragmentation on other tumor cell lines : HeLa, Meth A, and B16F1 cells, but not on normal human diploid fibroblasts FS-4. These results suggest that β-thujaplicin-zinc chelate induces apoptotic cell death in various tumor cell lines and is a potent antitumor agent for tumor cells including malignant melanomas. |
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In addition, the zinc chelate induced DNA ladder formation in B16BL6 cells, as shown by the DNA fragmentation assay, suggesting that cell death induced by the zinc chelate is apoptosis. The zinc chelate also had a cytotoxic effect and induced DNA fragmentation on other tumor cell lines : HeLa, Meth A, and B16F1 cells, but not on normal human diploid fibroblasts FS-4. These results suggest that β-thujaplicin-zinc chelate induces apoptotic cell death in various tumor cell lines and is a potent antitumor agent for tumor cells including malignant melanomas.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.21.1258</identifier><identifier>PMID: 9881634</identifier><language>eng</language><publisher>Tokyo: The Pharmaceutical Society of Japan</publisher><subject>Animals ; Antineoplastic Agents - pharmacology ; Apoptosis ; B16BL6 melanoma ; Biological and medical sciences ; Cell Division - drug effects ; Cell Line ; Chelating Agents - pharmacology ; DNA Fragmentation - drug effects ; DNA ladder formation ; General pharmacology ; HeLa Cells ; Humans ; Iron Chelating Agents - pharmacology ; Medical sciences ; Melanoma, Experimental - drug therapy ; Melanoma, Experimental - pathology ; Melanoma, Experimental - secondary ; Mice ; Monoterpenes ; Neoplasm Metastasis ; Pharmacognosy. Homeopathy. Health food ; Pharmacology. Drug treatments ; Tropolone - analogs & derivatives ; Tropolone - pharmacology ; Tumor Cells, Cultured ; Zinc - pharmacology ; β-thujaplicin ; β-thujaplicin-zinc chelate</subject><ispartof>Biological and Pharmaceutical Bulletin, 1998/12/15, Vol.21(12), pp.1258-1262</ispartof><rights>The Pharmaceutical Society of Japan</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c580t-c52aa53c58f238f7dcce134c5d1a144347f016b4be56c3f28b05378e31329eb03</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1680315$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9881634$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MIYAMOTO, Daisei</creatorcontrib><creatorcontrib>ENDO, Naoko</creatorcontrib><creatorcontrib>OKU, Naoto</creatorcontrib><creatorcontrib>ARIMA, Yaeno</creatorcontrib><creatorcontrib>SUZUKI, Takashi</creatorcontrib><creatorcontrib>SUZUKI, Yasuo</creatorcontrib><title>β-Thujaplicin Zinc Chelate Induces Apoptosis in Mouse High Metastatic Melanoma B16BL6 Cells</title><title>Biological & pharmaceutical bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>The cytotoxic effects of β-thujaplicin and five kinds of metal chelates were examined on mouse melanoma B16BL6 cells by cell viability and lactate dehydrogenase (LDH) release assay. β-Thujaplicin-zinc chalate and β-thujaplicin-copper chelate had higher cytotoxic effects than β-thujaplicin, and the 50% effective does (ED50) of these metal chelates were 12.5 and 25 μM, respectively. In addition, the zinc chelate induced DNA ladder formation in B16BL6 cells, as shown by the DNA fragmentation assay, suggesting that cell death induced by the zinc chelate is apoptosis. The zinc chelate also had a cytotoxic effect and induced DNA fragmentation on other tumor cell lines : HeLa, Meth A, and B16F1 cells, but not on normal human diploid fibroblasts FS-4. These results suggest that β-thujaplicin-zinc chelate induces apoptotic cell death in various tumor cell lines and is a potent antitumor agent for tumor cells including malignant melanomas.</description><subject>Animals</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Apoptosis</subject><subject>B16BL6 melanoma</subject><subject>Biological and medical sciences</subject><subject>Cell Division - drug effects</subject><subject>Cell Line</subject><subject>Chelating Agents - pharmacology</subject><subject>DNA Fragmentation - drug effects</subject><subject>DNA ladder formation</subject><subject>General pharmacology</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Iron Chelating Agents - pharmacology</subject><subject>Medical sciences</subject><subject>Melanoma, Experimental - drug therapy</subject><subject>Melanoma, Experimental - pathology</subject><subject>Melanoma, Experimental - secondary</subject><subject>Mice</subject><subject>Monoterpenes</subject><subject>Neoplasm Metastasis</subject><subject>Pharmacognosy. Homeopathy. Health food</subject><subject>Pharmacology. Drug treatments</subject><subject>Tropolone - analogs & derivatives</subject><subject>Tropolone - pharmacology</subject><subject>Tumor Cells, Cultured</subject><subject>Zinc - pharmacology</subject><subject>β-thujaplicin</subject><subject>β-thujaplicin-zinc chelate</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkM9q3DAQxkVpSTdJTzkHdCi9BKcayZLlY7I0f2BDL8mlFMRYO85q8dquZR_yWn2QPlO0rNleRiO-H_PNfIxdgLgGmdvvVV9dS0i9th_YAlReZFqC_sgWogSbGdD2MzuNcSuEKIRUJ-yktBaMyhfs97-_2fNm2mLfBB9a_iu0ni831OBI_LFdT54iv-m7fuxiiDwRT90UiT-E1w1_ohHjiGPwqW2w7XbIb8HcrgxfUtPEc_apxibSl_k9Yy93P56XD9nq5_3j8maVeW3FmKpE1Cp9aqlsXay9p3SF12tAyPN0Ty3AVHlF2nhVS1sJrQpLCpQsqRLqjH07zO2H7s9EcXS7EH3aAFtK2zpTgiyMtAm8OoB-6GIcqHb9EHY4vDkQbp-lS1k6CW6fZaIv57FTtaP1kZ3DS_rXWcfosakHbH2I_0caKxTohN0dsG3K6pWOOg4puIb2llCW6mA7173_EfAbHBy16h2MxpOR</recordid><startdate>1998</startdate><enddate>1998</enddate><creator>MIYAMOTO, Daisei</creator><creator>ENDO, Naoko</creator><creator>OKU, Naoto</creator><creator>ARIMA, Yaeno</creator><creator>SUZUKI, Takashi</creator><creator>SUZUKI, Yasuo</creator><general>The Pharmaceutical Society of Japan</general><general>Maruzen</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1998</creationdate><title>β-Thujaplicin Zinc Chelate Induces Apoptosis in Mouse High Metastatic Melanoma B16BL6 Cells</title><author>MIYAMOTO, Daisei ; ENDO, Naoko ; OKU, Naoto ; ARIMA, Yaeno ; SUZUKI, Takashi ; SUZUKI, Yasuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c580t-c52aa53c58f238f7dcce134c5d1a144347f016b4be56c3f28b05378e31329eb03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Apoptosis</topic><topic>B16BL6 melanoma</topic><topic>Biological and medical sciences</topic><topic>Cell Division - drug effects</topic><topic>Cell Line</topic><topic>Chelating Agents - pharmacology</topic><topic>DNA Fragmentation - drug effects</topic><topic>DNA ladder formation</topic><topic>General pharmacology</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Iron Chelating Agents - pharmacology</topic><topic>Medical sciences</topic><topic>Melanoma, Experimental - drug therapy</topic><topic>Melanoma, Experimental - pathology</topic><topic>Melanoma, Experimental - secondary</topic><topic>Mice</topic><topic>Monoterpenes</topic><topic>Neoplasm Metastasis</topic><topic>Pharmacognosy. Homeopathy. Health food</topic><topic>Pharmacology. Drug treatments</topic><topic>Tropolone - analogs & derivatives</topic><topic>Tropolone - pharmacology</topic><topic>Tumor Cells, Cultured</topic><topic>Zinc - pharmacology</topic><topic>β-thujaplicin</topic><topic>β-thujaplicin-zinc chelate</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MIYAMOTO, Daisei</creatorcontrib><creatorcontrib>ENDO, Naoko</creatorcontrib><creatorcontrib>OKU, Naoto</creatorcontrib><creatorcontrib>ARIMA, Yaeno</creatorcontrib><creatorcontrib>SUZUKI, Takashi</creatorcontrib><creatorcontrib>SUZUKI, Yasuo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biological & pharmaceutical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MIYAMOTO, Daisei</au><au>ENDO, Naoko</au><au>OKU, Naoto</au><au>ARIMA, Yaeno</au><au>SUZUKI, Takashi</au><au>SUZUKI, Yasuo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>β-Thujaplicin Zinc Chelate Induces Apoptosis in Mouse High Metastatic Melanoma B16BL6 Cells</atitle><jtitle>Biological & pharmaceutical bulletin</jtitle><addtitle>Biol Pharm Bull</addtitle><date>1998</date><risdate>1998</risdate><volume>21</volume><issue>12</issue><spage>1258</spage><epage>1262</epage><pages>1258-1262</pages><issn>0918-6158</issn><eissn>1347-5215</eissn><abstract>The cytotoxic effects of β-thujaplicin and five kinds of metal chelates were examined on mouse melanoma B16BL6 cells by cell viability and lactate dehydrogenase (LDH) release assay. β-Thujaplicin-zinc chalate and β-thujaplicin-copper chelate had higher cytotoxic effects than β-thujaplicin, and the 50% effective does (ED50) of these metal chelates were 12.5 and 25 μM, respectively. In addition, the zinc chelate induced DNA ladder formation in B16BL6 cells, as shown by the DNA fragmentation assay, suggesting that cell death induced by the zinc chelate is apoptosis. The zinc chelate also had a cytotoxic effect and induced DNA fragmentation on other tumor cell lines : HeLa, Meth A, and B16F1 cells, but not on normal human diploid fibroblasts FS-4. These results suggest that β-thujaplicin-zinc chelate induces apoptotic cell death in various tumor cell lines and is a potent antitumor agent for tumor cells including malignant melanomas.</abstract><cop>Tokyo</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>9881634</pmid><doi>10.1248/bpb.21.1258</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animals Antineoplastic Agents - pharmacology Apoptosis B16BL6 melanoma Biological and medical sciences Cell Division - drug effects Cell Line Chelating Agents - pharmacology DNA Fragmentation - drug effects DNA ladder formation General pharmacology HeLa Cells Humans Iron Chelating Agents - pharmacology Medical sciences Melanoma, Experimental - drug therapy Melanoma, Experimental - pathology Melanoma, Experimental - secondary Mice Monoterpenes Neoplasm Metastasis Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Tropolone - analogs & derivatives Tropolone - pharmacology Tumor Cells, Cultured Zinc - pharmacology β-thujaplicin β-thujaplicin-zinc chelate |
title | β-Thujaplicin Zinc Chelate Induces Apoptosis in Mouse High Metastatic Melanoma B16BL6 Cells |
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