β-Thujaplicin Zinc Chelate Induces Apoptosis in Mouse High Metastatic Melanoma B16BL6 Cells

The cytotoxic effects of β-thujaplicin and five kinds of metal chelates were examined on mouse melanoma B16BL6 cells by cell viability and lactate dehydrogenase (LDH) release assay. β-Thujaplicin-zinc chalate and β-thujaplicin-copper chelate had higher cytotoxic effects than β-thujaplicin, and the 5...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Biological & pharmaceutical bulletin 1998/12/15, Vol.21(12), pp.1258-1262
Hauptverfasser: MIYAMOTO, Daisei, ENDO, Naoko, OKU, Naoto, ARIMA, Yaeno, SUZUKI, Takashi, SUZUKI, Yasuo
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 1262
container_issue 12
container_start_page 1258
container_title Biological & pharmaceutical bulletin
container_volume 21
creator MIYAMOTO, Daisei
ENDO, Naoko
OKU, Naoto
ARIMA, Yaeno
SUZUKI, Takashi
SUZUKI, Yasuo
description The cytotoxic effects of β-thujaplicin and five kinds of metal chelates were examined on mouse melanoma B16BL6 cells by cell viability and lactate dehydrogenase (LDH) release assay. β-Thujaplicin-zinc chalate and β-thujaplicin-copper chelate had higher cytotoxic effects than β-thujaplicin, and the 50% effective does (ED50) of these metal chelates were 12.5 and 25 μM, respectively. In addition, the zinc chelate induced DNA ladder formation in B16BL6 cells, as shown by the DNA fragmentation assay, suggesting that cell death induced by the zinc chelate is apoptosis. The zinc chelate also had a cytotoxic effect and induced DNA fragmentation on other tumor cell lines : HeLa, Meth A, and B16F1 cells, but not on normal human diploid fibroblasts FS-4. These results suggest that β-thujaplicin-zinc chelate induces apoptotic cell death in various tumor cell lines and is a potent antitumor agent for tumor cells including malignant melanomas.
doi_str_mv 10.1248/bpb.21.1258
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69127628</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>69127628</sourcerecordid><originalsourceid>FETCH-LOGICAL-c580t-c52aa53c58f238f7dcce134c5d1a144347f016b4be56c3f28b05378e31329eb03</originalsourceid><addsrcrecordid>eNpFkM9q3DAQxkVpSTdJTzkHdCi9BKcayZLlY7I0f2BDL8mlFMRYO85q8dquZR_yWn2QPlO0rNleRiO-H_PNfIxdgLgGmdvvVV9dS0i9th_YAlReZFqC_sgWogSbGdD2MzuNcSuEKIRUJ-yktBaMyhfs97-_2fNm2mLfBB9a_iu0ni831OBI_LFdT54iv-m7fuxiiDwRT90UiT-E1w1_ohHjiGPwqW2w7XbIb8HcrgxfUtPEc_apxibSl_k9Yy93P56XD9nq5_3j8maVeW3FmKpE1Cp9aqlsXay9p3SF12tAyPN0Ty3AVHlF2nhVS1sJrQpLCpQsqRLqjH07zO2H7s9EcXS7EH3aAFtK2zpTgiyMtAm8OoB-6GIcqHb9EHY4vDkQbp-lS1k6CW6fZaIv57FTtaP1kZ3DS_rXWcfosakHbH2I_0caKxTohN0dsG3K6pWOOg4puIb2llCW6mA7173_EfAbHBy16h2MxpOR</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69127628</pqid></control><display><type>article</type><title>β-Thujaplicin Zinc Chelate Induces Apoptosis in Mouse High Metastatic Melanoma B16BL6 Cells</title><source>J-STAGE Free</source><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Free Full-Text Journals in Chemistry</source><creator>MIYAMOTO, Daisei ; ENDO, Naoko ; OKU, Naoto ; ARIMA, Yaeno ; SUZUKI, Takashi ; SUZUKI, Yasuo</creator><creatorcontrib>MIYAMOTO, Daisei ; ENDO, Naoko ; OKU, Naoto ; ARIMA, Yaeno ; SUZUKI, Takashi ; SUZUKI, Yasuo</creatorcontrib><description>The cytotoxic effects of β-thujaplicin and five kinds of metal chelates were examined on mouse melanoma B16BL6 cells by cell viability and lactate dehydrogenase (LDH) release assay. β-Thujaplicin-zinc chalate and β-thujaplicin-copper chelate had higher cytotoxic effects than β-thujaplicin, and the 50% effective does (ED50) of these metal chelates were 12.5 and 25 μM, respectively. In addition, the zinc chelate induced DNA ladder formation in B16BL6 cells, as shown by the DNA fragmentation assay, suggesting that cell death induced by the zinc chelate is apoptosis. The zinc chelate also had a cytotoxic effect and induced DNA fragmentation on other tumor cell lines : HeLa, Meth A, and B16F1 cells, but not on normal human diploid fibroblasts FS-4. These results suggest that β-thujaplicin-zinc chelate induces apoptotic cell death in various tumor cell lines and is a potent antitumor agent for tumor cells including malignant melanomas.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.21.1258</identifier><identifier>PMID: 9881634</identifier><language>eng</language><publisher>Tokyo: The Pharmaceutical Society of Japan</publisher><subject>Animals ; Antineoplastic Agents - pharmacology ; Apoptosis ; B16BL6 melanoma ; Biological and medical sciences ; Cell Division - drug effects ; Cell Line ; Chelating Agents - pharmacology ; DNA Fragmentation - drug effects ; DNA ladder formation ; General pharmacology ; HeLa Cells ; Humans ; Iron Chelating Agents - pharmacology ; Medical sciences ; Melanoma, Experimental - drug therapy ; Melanoma, Experimental - pathology ; Melanoma, Experimental - secondary ; Mice ; Monoterpenes ; Neoplasm Metastasis ; Pharmacognosy. Homeopathy. Health food ; Pharmacology. Drug treatments ; Tropolone - analogs &amp; derivatives ; Tropolone - pharmacology ; Tumor Cells, Cultured ; Zinc - pharmacology ; β-thujaplicin ; β-thujaplicin-zinc chelate</subject><ispartof>Biological and Pharmaceutical Bulletin, 1998/12/15, Vol.21(12), pp.1258-1262</ispartof><rights>The Pharmaceutical Society of Japan</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c580t-c52aa53c58f238f7dcce134c5d1a144347f016b4be56c3f28b05378e31329eb03</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=1680315$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9881634$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MIYAMOTO, Daisei</creatorcontrib><creatorcontrib>ENDO, Naoko</creatorcontrib><creatorcontrib>OKU, Naoto</creatorcontrib><creatorcontrib>ARIMA, Yaeno</creatorcontrib><creatorcontrib>SUZUKI, Takashi</creatorcontrib><creatorcontrib>SUZUKI, Yasuo</creatorcontrib><title>β-Thujaplicin Zinc Chelate Induces Apoptosis in Mouse High Metastatic Melanoma B16BL6 Cells</title><title>Biological &amp; pharmaceutical bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>The cytotoxic effects of β-thujaplicin and five kinds of metal chelates were examined on mouse melanoma B16BL6 cells by cell viability and lactate dehydrogenase (LDH) release assay. β-Thujaplicin-zinc chalate and β-thujaplicin-copper chelate had higher cytotoxic effects than β-thujaplicin, and the 50% effective does (ED50) of these metal chelates were 12.5 and 25 μM, respectively. In addition, the zinc chelate induced DNA ladder formation in B16BL6 cells, as shown by the DNA fragmentation assay, suggesting that cell death induced by the zinc chelate is apoptosis. The zinc chelate also had a cytotoxic effect and induced DNA fragmentation on other tumor cell lines : HeLa, Meth A, and B16F1 cells, but not on normal human diploid fibroblasts FS-4. These results suggest that β-thujaplicin-zinc chelate induces apoptotic cell death in various tumor cell lines and is a potent antitumor agent for tumor cells including malignant melanomas.</description><subject>Animals</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Apoptosis</subject><subject>B16BL6 melanoma</subject><subject>Biological and medical sciences</subject><subject>Cell Division - drug effects</subject><subject>Cell Line</subject><subject>Chelating Agents - pharmacology</subject><subject>DNA Fragmentation - drug effects</subject><subject>DNA ladder formation</subject><subject>General pharmacology</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Iron Chelating Agents - pharmacology</subject><subject>Medical sciences</subject><subject>Melanoma, Experimental - drug therapy</subject><subject>Melanoma, Experimental - pathology</subject><subject>Melanoma, Experimental - secondary</subject><subject>Mice</subject><subject>Monoterpenes</subject><subject>Neoplasm Metastasis</subject><subject>Pharmacognosy. Homeopathy. Health food</subject><subject>Pharmacology. Drug treatments</subject><subject>Tropolone - analogs &amp; derivatives</subject><subject>Tropolone - pharmacology</subject><subject>Tumor Cells, Cultured</subject><subject>Zinc - pharmacology</subject><subject>β-thujaplicin</subject><subject>β-thujaplicin-zinc chelate</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkM9q3DAQxkVpSTdJTzkHdCi9BKcayZLlY7I0f2BDL8mlFMRYO85q8dquZR_yWn2QPlO0rNleRiO-H_PNfIxdgLgGmdvvVV9dS0i9th_YAlReZFqC_sgWogSbGdD2MzuNcSuEKIRUJ-yktBaMyhfs97-_2fNm2mLfBB9a_iu0ni831OBI_LFdT54iv-m7fuxiiDwRT90UiT-E1w1_ohHjiGPwqW2w7XbIb8HcrgxfUtPEc_apxibSl_k9Yy93P56XD9nq5_3j8maVeW3FmKpE1Cp9aqlsXay9p3SF12tAyPN0Ty3AVHlF2nhVS1sJrQpLCpQsqRLqjH07zO2H7s9EcXS7EH3aAFtK2zpTgiyMtAm8OoB-6GIcqHb9EHY4vDkQbp-lS1k6CW6fZaIv57FTtaP1kZ3DS_rXWcfosakHbH2I_0caKxTohN0dsG3K6pWOOg4puIb2llCW6mA7173_EfAbHBy16h2MxpOR</recordid><startdate>1998</startdate><enddate>1998</enddate><creator>MIYAMOTO, Daisei</creator><creator>ENDO, Naoko</creator><creator>OKU, Naoto</creator><creator>ARIMA, Yaeno</creator><creator>SUZUKI, Takashi</creator><creator>SUZUKI, Yasuo</creator><general>The Pharmaceutical Society of Japan</general><general>Maruzen</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1998</creationdate><title>β-Thujaplicin Zinc Chelate Induces Apoptosis in Mouse High Metastatic Melanoma B16BL6 Cells</title><author>MIYAMOTO, Daisei ; ENDO, Naoko ; OKU, Naoto ; ARIMA, Yaeno ; SUZUKI, Takashi ; SUZUKI, Yasuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c580t-c52aa53c58f238f7dcce134c5d1a144347f016b4be56c3f28b05378e31329eb03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Apoptosis</topic><topic>B16BL6 melanoma</topic><topic>Biological and medical sciences</topic><topic>Cell Division - drug effects</topic><topic>Cell Line</topic><topic>Chelating Agents - pharmacology</topic><topic>DNA Fragmentation - drug effects</topic><topic>DNA ladder formation</topic><topic>General pharmacology</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Iron Chelating Agents - pharmacology</topic><topic>Medical sciences</topic><topic>Melanoma, Experimental - drug therapy</topic><topic>Melanoma, Experimental - pathology</topic><topic>Melanoma, Experimental - secondary</topic><topic>Mice</topic><topic>Monoterpenes</topic><topic>Neoplasm Metastasis</topic><topic>Pharmacognosy. Homeopathy. Health food</topic><topic>Pharmacology. Drug treatments</topic><topic>Tropolone - analogs &amp; derivatives</topic><topic>Tropolone - pharmacology</topic><topic>Tumor Cells, Cultured</topic><topic>Zinc - pharmacology</topic><topic>β-thujaplicin</topic><topic>β-thujaplicin-zinc chelate</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MIYAMOTO, Daisei</creatorcontrib><creatorcontrib>ENDO, Naoko</creatorcontrib><creatorcontrib>OKU, Naoto</creatorcontrib><creatorcontrib>ARIMA, Yaeno</creatorcontrib><creatorcontrib>SUZUKI, Takashi</creatorcontrib><creatorcontrib>SUZUKI, Yasuo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biological &amp; pharmaceutical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MIYAMOTO, Daisei</au><au>ENDO, Naoko</au><au>OKU, Naoto</au><au>ARIMA, Yaeno</au><au>SUZUKI, Takashi</au><au>SUZUKI, Yasuo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>β-Thujaplicin Zinc Chelate Induces Apoptosis in Mouse High Metastatic Melanoma B16BL6 Cells</atitle><jtitle>Biological &amp; pharmaceutical bulletin</jtitle><addtitle>Biol Pharm Bull</addtitle><date>1998</date><risdate>1998</risdate><volume>21</volume><issue>12</issue><spage>1258</spage><epage>1262</epage><pages>1258-1262</pages><issn>0918-6158</issn><eissn>1347-5215</eissn><abstract>The cytotoxic effects of β-thujaplicin and five kinds of metal chelates were examined on mouse melanoma B16BL6 cells by cell viability and lactate dehydrogenase (LDH) release assay. β-Thujaplicin-zinc chalate and β-thujaplicin-copper chelate had higher cytotoxic effects than β-thujaplicin, and the 50% effective does (ED50) of these metal chelates were 12.5 and 25 μM, respectively. In addition, the zinc chelate induced DNA ladder formation in B16BL6 cells, as shown by the DNA fragmentation assay, suggesting that cell death induced by the zinc chelate is apoptosis. The zinc chelate also had a cytotoxic effect and induced DNA fragmentation on other tumor cell lines : HeLa, Meth A, and B16F1 cells, but not on normal human diploid fibroblasts FS-4. These results suggest that β-thujaplicin-zinc chelate induces apoptotic cell death in various tumor cell lines and is a potent antitumor agent for tumor cells including malignant melanomas.</abstract><cop>Tokyo</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>9881634</pmid><doi>10.1248/bpb.21.1258</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0918-6158
ispartof Biological and Pharmaceutical Bulletin, 1998/12/15, Vol.21(12), pp.1258-1262
issn 0918-6158
1347-5215
language eng
recordid cdi_proquest_miscellaneous_69127628
source J-STAGE Free; MEDLINE; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry
subjects Animals
Antineoplastic Agents - pharmacology
Apoptosis
B16BL6 melanoma
Biological and medical sciences
Cell Division - drug effects
Cell Line
Chelating Agents - pharmacology
DNA Fragmentation - drug effects
DNA ladder formation
General pharmacology
HeLa Cells
Humans
Iron Chelating Agents - pharmacology
Medical sciences
Melanoma, Experimental - drug therapy
Melanoma, Experimental - pathology
Melanoma, Experimental - secondary
Mice
Monoterpenes
Neoplasm Metastasis
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Tropolone - analogs & derivatives
Tropolone - pharmacology
Tumor Cells, Cultured
Zinc - pharmacology
β-thujaplicin
β-thujaplicin-zinc chelate
title β-Thujaplicin Zinc Chelate Induces Apoptosis in Mouse High Metastatic Melanoma B16BL6 Cells
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T11%3A59%3A40IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=%CE%B2-Thujaplicin%20Zinc%20Chelate%20Induces%20Apoptosis%20in%20Mouse%20High%20Metastatic%20Melanoma%20B16BL6%20Cells&rft.jtitle=Biological%20&%20pharmaceutical%20bulletin&rft.au=MIYAMOTO,%20Daisei&rft.date=1998&rft.volume=21&rft.issue=12&rft.spage=1258&rft.epage=1262&rft.pages=1258-1262&rft.issn=0918-6158&rft.eissn=1347-5215&rft_id=info:doi/10.1248/bpb.21.1258&rft_dat=%3Cproquest_cross%3E69127628%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=69127628&rft_id=info:pmid/9881634&rfr_iscdi=true