Analysis of enterohemorrhagic Escherichia coli serotype O157:H7 by flow cytometry using monoclonal antibodies
To develop a rapid and specific method to detect and/or identify enterohemorrhagic Escherichia coli O157:H7, two mouse monoclonal antibodies (MAbs) were prepared. Specificities of these two MAbs (1D9 and 3E8) were determined by flow cytometry method (FCM). MAbs 3E8 and 1D9 were found to react with E...
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| Veröffentlicht in: | Journal of Veterinary Medical Science 1998, Vol.60(12), pp.1315-1319 |
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| container_title | Journal of Veterinary Medical Science |
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| creator | Kusunoki, H. (Osaka Prefectural Univ., Sakai (Japan). Coll. of Agriculture) Kobayashi, K Kita, T Tajima, T Sugii, S Uemura, T |
| description | To develop a rapid and specific method to detect and/or identify enterohemorrhagic Escherichia coli O157:H7, two mouse monoclonal antibodies (MAbs) were prepared. Specificities of these two MAbs (1D9 and 3E8) were determined by flow cytometry method (FCM). MAbs 3E8 and 1D9 were found to react with E. coli O157:H7, Citrobacter freundii and Salmonella group N (O:30), but not with Escherichia hermannii. With a mixture containing strains of E. coli O157:H7 and E. coli O6:H1, two different peaks appeared in FCM with MAbs, whereas a single peak appeared with polyclonal rabbit antiserum. From these findings, FCM with MAb is suggested to be a rapid, specific, and useful method to detect and identify strains(s) of E. coli O157:H7 in food ingredients |
| doi_str_mv | 10.1292/jvms.60.1315 |
| format | Article |
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(Osaka Prefectural Univ., Sakai (Japan). Coll. of Agriculture) ; Kobayashi, K ; Kita, T ; Tajima, T ; Sugii, S ; Uemura, T</creator><creatorcontrib>Kusunoki, H. (Osaka Prefectural Univ., Sakai (Japan). Coll. of Agriculture) ; Kobayashi, K ; Kita, T ; Tajima, T ; Sugii, S ; Uemura, T</creatorcontrib><description>To develop a rapid and specific method to detect and/or identify enterohemorrhagic Escherichia coli O157:H7, two mouse monoclonal antibodies (MAbs) were prepared. Specificities of these two MAbs (1D9 and 3E8) were determined by flow cytometry method (FCM). MAbs 3E8 and 1D9 were found to react with E. coli O157:H7, Citrobacter freundii and Salmonella group N (O:30), but not with Escherichia hermannii. With a mixture containing strains of E. coli O157:H7 and E. coli O6:H1, two different peaks appeared in FCM with MAbs, whereas a single peak appeared with polyclonal rabbit antiserum. From these findings, FCM with MAb is suggested to be a rapid, specific, and useful method to detect and identify strains(s) of E. coli O157:H7 in food ingredients</description><identifier>ISSN: 0916-7250</identifier><identifier>EISSN: 1347-7439</identifier><identifier>DOI: 10.1292/jvms.60.1315</identifier><identifier>PMID: 9879532</identifier><language>eng</language><publisher>Japan: JAPANESE SOCIETY OF VETERINARY SCIENCE</publisher><subject>Animals ; Antibodies, Monoclonal ; BACTERIAL TOXINS ; Binding, Competitive ; CELL COUNTING ; Citrobacter freundii ; Citrobacter freundii - immunology ; CONTEO DE CELULAS ; Escherichia - immunology ; ESCHERICHIA COLI ; Escherichia coli O157 - immunology ; Escherichia coli O157 - isolation & purification ; FCM ; Flow Cytometry - veterinary ; MICE ; NUMERATION CELLULAIRE ; O157 ; Rabbits ; RATON ; Salmonella ; Salmonella - immunology ; Sensitivity and Specificity ; SOURIS ; TOXINAS BACTERIANAS ; TOXINE BACTERIENNE</subject><ispartof>Journal of Veterinary Medical Science, 1998, Vol.60(12), pp.1315-1319</ispartof><rights>1998 by the Japanese Society of Veterinary Science</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c538t-731c304580bb123a228830b8a0fa9aac5aa845ccb1ef75c7896617f1f67c87e63</citedby><cites>FETCH-LOGICAL-c538t-731c304580bb123a228830b8a0fa9aac5aa845ccb1ef75c7896617f1f67c87e63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1876,4009,27902,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9879532$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kusunoki, H. (Osaka Prefectural Univ., Sakai (Japan). Coll. of Agriculture)</creatorcontrib><creatorcontrib>Kobayashi, K</creatorcontrib><creatorcontrib>Kita, T</creatorcontrib><creatorcontrib>Tajima, T</creatorcontrib><creatorcontrib>Sugii, S</creatorcontrib><creatorcontrib>Uemura, T</creatorcontrib><title>Analysis of enterohemorrhagic Escherichia coli serotype O157:H7 by flow cytometry using monoclonal antibodies</title><title>Journal of Veterinary Medical Science</title><addtitle>J. Vet. Med. Sci.</addtitle><description>To develop a rapid and specific method to detect and/or identify enterohemorrhagic Escherichia coli O157:H7, two mouse monoclonal antibodies (MAbs) were prepared. Specificities of these two MAbs (1D9 and 3E8) were determined by flow cytometry method (FCM). MAbs 3E8 and 1D9 were found to react with E. coli O157:H7, Citrobacter freundii and Salmonella group N (O:30), but not with Escherichia hermannii. With a mixture containing strains of E. coli O157:H7 and E. coli O6:H1, two different peaks appeared in FCM with MAbs, whereas a single peak appeared with polyclonal rabbit antiserum. From these findings, FCM with MAb is suggested to be a rapid, specific, and useful method to detect and identify strains(s) of E. coli O157:H7 in food ingredients</description><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>BACTERIAL TOXINS</subject><subject>Binding, Competitive</subject><subject>CELL COUNTING</subject><subject>Citrobacter freundii</subject><subject>Citrobacter freundii - immunology</subject><subject>CONTEO DE CELULAS</subject><subject>Escherichia - immunology</subject><subject>ESCHERICHIA COLI</subject><subject>Escherichia coli O157 - immunology</subject><subject>Escherichia coli O157 - isolation & purification</subject><subject>FCM</subject><subject>Flow Cytometry - veterinary</subject><subject>MICE</subject><subject>NUMERATION CELLULAIRE</subject><subject>O157</subject><subject>Rabbits</subject><subject>RATON</subject><subject>Salmonella</subject><subject>Salmonella - immunology</subject><subject>Sensitivity and Specificity</subject><subject>SOURIS</subject><subject>TOXINAS BACTERIANAS</subject><subject>TOXINE BACTERIENNE</subject><issn>0916-7250</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtvEzEURi0EKmlhxxbJK1ZM8WP8YldFhYIqlQWsrTuunXE0Mw62UzT_nokSpcturnX1HZ0r-UPoAyXXlBn2Zfs0lmu5LJyKV2hFeasa1XLzGq2IobJRTJC36LKULSGMttJcoAujlRGcrdB4M8Ewl1hwCthP1efU-zHl3MMmOnxbXO9zdH0E7NIQcVmAOu88fqBCfb1TuJtxGNI_7OaaRl_zjPclThs8pim5IS12DFONXXqMvrxDbwIMxb8_vVfoz7fb3-u75v7h-4_1zX3jBNe1UZw6TlqhSddRxoExrTnpNJAABsAJAN0K5zrqgxJOaSMlVYEGqZxWXvIr9Ono3eX0d-9LtWMszg8DTD7ti5WGMsWJfhFkREojFV_Az0fQ5VRK9sHuchwhz5YSe6jBHmqwclmWGhb848m770b_eIZP_77k62O-LRU2_pxDrtEN_lnGTvNgPaeuh2z99HwlQLKwybHYn7-oMYYQ2dKW_wd-WqV3</recordid><startdate>1998</startdate><enddate>1998</enddate><creator>Kusunoki, H. (Osaka Prefectural Univ., Sakai (Japan). Coll. of Agriculture)</creator><creator>Kobayashi, K</creator><creator>Kita, T</creator><creator>Tajima, T</creator><creator>Sugii, S</creator><creator>Uemura, T</creator><general>JAPANESE SOCIETY OF VETERINARY SCIENCE</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>1998</creationdate><title>Analysis of enterohemorrhagic Escherichia coli serotype O157:H7 by flow cytometry using monoclonal antibodies</title><author>Kusunoki, H. (Osaka Prefectural Univ., Sakai (Japan). Coll. of Agriculture) ; Kobayashi, K ; Kita, T ; Tajima, T ; Sugii, S ; Uemura, T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c538t-731c304580bb123a228830b8a0fa9aac5aa845ccb1ef75c7896617f1f67c87e63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>BACTERIAL TOXINS</topic><topic>Binding, Competitive</topic><topic>CELL COUNTING</topic><topic>Citrobacter freundii</topic><topic>Citrobacter freundii - immunology</topic><topic>CONTEO DE CELULAS</topic><topic>Escherichia - immunology</topic><topic>ESCHERICHIA COLI</topic><topic>Escherichia coli O157 - immunology</topic><topic>Escherichia coli O157 - isolation & purification</topic><topic>FCM</topic><topic>Flow Cytometry - veterinary</topic><topic>MICE</topic><topic>NUMERATION CELLULAIRE</topic><topic>O157</topic><topic>Rabbits</topic><topic>RATON</topic><topic>Salmonella</topic><topic>Salmonella - immunology</topic><topic>Sensitivity and Specificity</topic><topic>SOURIS</topic><topic>TOXINAS BACTERIANAS</topic><topic>TOXINE BACTERIENNE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kusunoki, H. (Osaka Prefectural Univ., Sakai (Japan). Coll. of Agriculture)</creatorcontrib><creatorcontrib>Kobayashi, K</creatorcontrib><creatorcontrib>Kita, T</creatorcontrib><creatorcontrib>Tajima, T</creatorcontrib><creatorcontrib>Sugii, S</creatorcontrib><creatorcontrib>Uemura, T</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Veterinary Medical Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kusunoki, H. (Osaka Prefectural Univ., Sakai (Japan). Coll. of Agriculture)</au><au>Kobayashi, K</au><au>Kita, T</au><au>Tajima, T</au><au>Sugii, S</au><au>Uemura, T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of enterohemorrhagic Escherichia coli serotype O157:H7 by flow cytometry using monoclonal antibodies</atitle><jtitle>Journal of Veterinary Medical Science</jtitle><addtitle>J. Vet. Med. Sci.</addtitle><date>1998</date><risdate>1998</risdate><volume>60</volume><issue>12</issue><spage>1315</spage><epage>1319</epage><pages>1315-1319</pages><issn>0916-7250</issn><eissn>1347-7439</eissn><abstract>To develop a rapid and specific method to detect and/or identify enterohemorrhagic Escherichia coli O157:H7, two mouse monoclonal antibodies (MAbs) were prepared. Specificities of these two MAbs (1D9 and 3E8) were determined by flow cytometry method (FCM). MAbs 3E8 and 1D9 were found to react with E. coli O157:H7, Citrobacter freundii and Salmonella group N (O:30), but not with Escherichia hermannii. With a mixture containing strains of E. coli O157:H7 and E. coli O6:H1, two different peaks appeared in FCM with MAbs, whereas a single peak appeared with polyclonal rabbit antiserum. From these findings, FCM with MAb is suggested to be a rapid, specific, and useful method to detect and identify strains(s) of E. coli O157:H7 in food ingredients</abstract><cop>Japan</cop><pub>JAPANESE SOCIETY OF VETERINARY SCIENCE</pub><pmid>9879532</pmid><doi>10.1292/jvms.60.1315</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | J-STAGE Free; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Antibodies, Monoclonal BACTERIAL TOXINS Binding, Competitive CELL COUNTING Citrobacter freundii Citrobacter freundii - immunology CONTEO DE CELULAS Escherichia - immunology ESCHERICHIA COLI Escherichia coli O157 - immunology Escherichia coli O157 - isolation & purification FCM Flow Cytometry - veterinary MICE NUMERATION CELLULAIRE O157 Rabbits RATON Salmonella Salmonella - immunology Sensitivity and Specificity SOURIS TOXINAS BACTERIANAS TOXINE BACTERIENNE |
| title | Analysis of enterohemorrhagic Escherichia coli serotype O157:H7 by flow cytometry using monoclonal antibodies |
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