Electrospray Ionization–Fourier Transform Ion Cyclotron Resonance Mass Spectrometry at 11.5 Tesla: Instrument Design and Initial Results
Initial results obtained using a new electrospray ionization (ESI) Fourier transform ion cyclotron resonance (FTICR) mass spectrometer operated at a magnetic field 11.5 tesla are presented. The new instrument utilized an electrostatic ion guide between the ESI source and FTICR trap that provided up...
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| Veröffentlicht in: | Journal of the American Society for Mass Spectrometry 1998-07, Vol.9 (7), p.692-700 |
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| creator | Gorshkov, Michael V. Paša Tolić, Ljiljana Udseth, Harold R. Anderson, Gordon A. Huang, Baoming M. Bruce, James E. Prior, David C. Hofstadler, Steven A. Tang, Liang Chen, Lin-Zhi Willett, Jesse A. Rockwood, Alan L. Sherman, Michael S. Smith, Richard D. |
| description | Initial results obtained using a new electrospray ionization (ESI) Fourier transform ion cyclotron resonance (FTICR) mass spectrometer operated at a magnetic field 11.5 tesla are presented. The new instrument utilized an electrostatic ion guide between the ESI source and FTICR trap that provided up to 5% overall transmission efficiency for light ions and up to 30% efficiency for heavier biomolecules. The higher magnetic field in combination with an enlarged FTICR ion trap made it possible to substantially improve resolving power and operate in a more robust fashion for large biopolymers compared to lower field instruments. Mass resolution up to 10
6 has been achieved for intermediate size biopolymers such as bovine ubiquitin (8.6 kDa) and bovine cytochrome
c (12.4 kDa) without the use of frequency drift correction methods. A mass resolution of 370,000 has been demonstrated for isotopically resolved molecular ions of bovine serum albumin (66.5 kDa). Comparative measurements were made with the same spectrometer using a lower field 3.5-tesla magnet allowing the performance gains to be more readily quantified. Further improvements in pumping capacity of the vacuum system and efficiency of ion transmission from the source are expected to lead to further substantial sensitivity gains. |
| doi_str_mv | 10.1016/S1044-0305(98)00037-3 |
| format | Article |
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6 has been achieved for intermediate size biopolymers such as bovine ubiquitin (8.6 kDa) and bovine cytochrome
c (12.4 kDa) without the use of frequency drift correction methods. A mass resolution of 370,000 has been demonstrated for isotopically resolved molecular ions of bovine serum albumin (66.5 kDa). Comparative measurements were made with the same spectrometer using a lower field 3.5-tesla magnet allowing the performance gains to be more readily quantified. Further improvements in pumping capacity of the vacuum system and efficiency of ion transmission from the source are expected to lead to further substantial sensitivity gains.</description><identifier>ISSN: 1044-0305</identifier><identifier>EISSN: 1879-1123</identifier><identifier>DOI: 10.1016/S1044-0305(98)00037-3</identifier><identifier>PMID: 9879379</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Algorithms ; Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; Biomolecules ; Biopolymers ; Cattle ; Cyclotron resonance ; Cyclotrons ; Efficiency ; Electromagnetic Fields ; Electrospraying ; ESI, FTICR, mass spectrometry, tesla, molecular mass range ; Exact sciences and technology ; Fourier Analysis ; Fourier transforms ; Frequency drift ; Fundamental and applied biological sciences. Psychology ; General aspects, investigation methods ; Instruments, apparatus, components and techniques common to several branches of physics and astronomy ; Ionization ; Ions ; Light ions ; Magnetic fields ; Magnetic resonance ; Mass spectrometers and related techniques ; Mass spectrometry ; Mass Spectrometry - instrumentation ; Mass Spectrometry - methods ; Molecular ions ; Physics ; Proteins ; Proteins - chemistry ; Resolution ; Serum albumin ; Transmission efficiency</subject><ispartof>Journal of the American Society for Mass Spectrometry, 1998-07, Vol.9 (7), p.692-700</ispartof><rights>1998 Elsevier Science Inc.</rights><rights>1998 INIST-CNRS</rights><rights>American Society for Mass Spectrometry 1998</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c444t-3e31f459dd046fa02d68589c17a73b1fd1d750e72bdeea6ea601fbf69407a72e3</citedby><cites>FETCH-LOGICAL-c444t-3e31f459dd046fa02d68589c17a73b1fd1d750e72bdeea6ea601fbf69407a72e3</cites><orcidid>0000000223812349 ; 0000000309366570</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,886,27929,27930</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2270906$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9879379$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/1545054$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Gorshkov, Michael V.</creatorcontrib><creatorcontrib>Paša Tolić, Ljiljana</creatorcontrib><creatorcontrib>Udseth, Harold R.</creatorcontrib><creatorcontrib>Anderson, Gordon A.</creatorcontrib><creatorcontrib>Huang, Baoming M.</creatorcontrib><creatorcontrib>Bruce, James E.</creatorcontrib><creatorcontrib>Prior, David C.</creatorcontrib><creatorcontrib>Hofstadler, Steven A.</creatorcontrib><creatorcontrib>Tang, Liang</creatorcontrib><creatorcontrib>Chen, Lin-Zhi</creatorcontrib><creatorcontrib>Willett, Jesse A.</creatorcontrib><creatorcontrib>Rockwood, Alan L.</creatorcontrib><creatorcontrib>Sherman, Michael S.</creatorcontrib><creatorcontrib>Smith, Richard D.</creatorcontrib><creatorcontrib>Pacific Northwest National Lab. (PNNL), Richland, WA (United States)</creatorcontrib><title>Electrospray Ionization–Fourier Transform Ion Cyclotron Resonance Mass Spectrometry at 11.5 Tesla: Instrument Design and Initial Results</title><title>Journal of the American Society for Mass Spectrometry</title><addtitle>J Am Soc Mass Spectrom</addtitle><description>Initial results obtained using a new electrospray ionization (ESI) Fourier transform ion cyclotron resonance (FTICR) mass spectrometer operated at a magnetic field 11.5 tesla are presented. The new instrument utilized an electrostatic ion guide between the ESI source and FTICR trap that provided up to 5% overall transmission efficiency for light ions and up to 30% efficiency for heavier biomolecules. The higher magnetic field in combination with an enlarged FTICR ion trap made it possible to substantially improve resolving power and operate in a more robust fashion for large biopolymers compared to lower field instruments. Mass resolution up to 10
6 has been achieved for intermediate size biopolymers such as bovine ubiquitin (8.6 kDa) and bovine cytochrome
c (12.4 kDa) without the use of frequency drift correction methods. A mass resolution of 370,000 has been demonstrated for isotopically resolved molecular ions of bovine serum albumin (66.5 kDa). Comparative measurements were made with the same spectrometer using a lower field 3.5-tesla magnet allowing the performance gains to be more readily quantified. Further improvements in pumping capacity of the vacuum system and efficiency of ion transmission from the source are expected to lead to further substantial sensitivity gains.</description><subject>Algorithms</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biomolecules</subject><subject>Biopolymers</subject><subject>Cattle</subject><subject>Cyclotron resonance</subject><subject>Cyclotrons</subject><subject>Efficiency</subject><subject>Electromagnetic Fields</subject><subject>Electrospraying</subject><subject>ESI, FTICR, mass spectrometry, tesla, molecular mass range</subject><subject>Exact sciences and technology</subject><subject>Fourier Analysis</subject><subject>Fourier transforms</subject><subject>Frequency drift</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects, investigation methods</subject><subject>Instruments, apparatus, components and techniques common to several branches of physics and astronomy</subject><subject>Ionization</subject><subject>Ions</subject><subject>Light ions</subject><subject>Magnetic fields</subject><subject>Magnetic resonance</subject><subject>Mass spectrometers and related techniques</subject><subject>Mass spectrometry</subject><subject>Mass Spectrometry - instrumentation</subject><subject>Mass Spectrometry - methods</subject><subject>Molecular ions</subject><subject>Physics</subject><subject>Proteins</subject><subject>Proteins - chemistry</subject><subject>Resolution</subject><subject>Serum albumin</subject><subject>Transmission efficiency</subject><issn>1044-0305</issn><issn>1879-1123</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkcGKFDEQQBtR1nX1ExaCiuih16STdHe8iIy7OrAiuOM5ZNLVmqU7mU3Swnjy7NU_9Eusnhn34EUIJKReFVX1iuKU0TNGWf3yilEhSsqpfK7aF5RS3pT8TnHM2kaVjFX8Lr7_IveLByldU8oaqpqj4kghxBt1XPw8H8DmGNImmi1ZBu--m-yC__3j10WYooNIVtH41Ic4zmGy2NohYIInnyAFb7wF8sGkRK42u0Ij5LglJhPGziRZQRrMK7L0KcdpBJ_JW0juiyfGd_jrsjPDXGgacnpY3OvNkODR4T4pPl-crxbvy8uP75aLN5elFULkkgNnvZCq66ioe0Orrm5lqyxrTMPXrO9Y10gKTbXuAEyNh7J-3ddKUCQq4CfF433dkLLTyboM9qsN3mP_mkkhqRQIPdtDmxhuJkhZjy5ZGAbjIUxJ14pVslIKwSf_gNe4N48DaKZkJThvqxYpuacsrjpF6PUmutHErWZUzzr1TqeeXWnV6p1OzTHv9FB9Wo_Q3WYd_GH86SFukjVDj6qsS7dYVaFvWiP2eo8B7vUbSp3HBlTXuThP3QX3n0b-AFLVvX8</recordid><startdate>19980701</startdate><enddate>19980701</enddate><creator>Gorshkov, Michael V.</creator><creator>Paša Tolić, Ljiljana</creator><creator>Udseth, Harold R.</creator><creator>Anderson, Gordon A.</creator><creator>Huang, Baoming M.</creator><creator>Bruce, James E.</creator><creator>Prior, David C.</creator><creator>Hofstadler, Steven A.</creator><creator>Tang, Liang</creator><creator>Chen, Lin-Zhi</creator><creator>Willett, Jesse A.</creator><creator>Rockwood, Alan L.</creator><creator>Sherman, Michael S.</creator><creator>Smith, Richard D.</creator><general>Elsevier Inc</general><general>Elsevier Science</general><general>Springer Nature B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FG</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>P5Z</scope><scope>P62</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>OTOTI</scope><orcidid>https://orcid.org/0000000223812349</orcidid><orcidid>https://orcid.org/0000000309366570</orcidid></search><sort><creationdate>19980701</creationdate><title>Electrospray Ionization–Fourier Transform Ion Cyclotron Resonance Mass Spectrometry at 11.5 Tesla: Instrument Design and Initial Results</title><author>Gorshkov, Michael V. ; Paša Tolić, Ljiljana ; Udseth, Harold R. ; Anderson, Gordon A. ; Huang, Baoming M. ; Bruce, James E. ; Prior, David C. ; Hofstadler, Steven A. ; Tang, Liang ; Chen, Lin-Zhi ; Willett, Jesse A. ; Rockwood, Alan L. ; Sherman, Michael S. ; Smith, Richard D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c444t-3e31f459dd046fa02d68589c17a73b1fd1d750e72bdeea6ea601fbf69407a72e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Algorithms</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biomolecules</topic><topic>Biopolymers</topic><topic>Cattle</topic><topic>Cyclotron resonance</topic><topic>Cyclotrons</topic><topic>Efficiency</topic><topic>Electromagnetic Fields</topic><topic>Electrospraying</topic><topic>ESI, FTICR, mass spectrometry, tesla, molecular mass range</topic><topic>Exact sciences and technology</topic><topic>Fourier Analysis</topic><topic>Fourier transforms</topic><topic>Frequency drift</topic><topic>Fundamental and applied biological sciences. 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(PNNL), Richland, WA (United States)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electrospray Ionization–Fourier Transform Ion Cyclotron Resonance Mass Spectrometry at 11.5 Tesla: Instrument Design and Initial Results</atitle><jtitle>Journal of the American Society for Mass Spectrometry</jtitle><addtitle>J Am Soc Mass Spectrom</addtitle><date>1998-07-01</date><risdate>1998</risdate><volume>9</volume><issue>7</issue><spage>692</spage><epage>700</epage><pages>692-700</pages><issn>1044-0305</issn><eissn>1879-1123</eissn><abstract>Initial results obtained using a new electrospray ionization (ESI) Fourier transform ion cyclotron resonance (FTICR) mass spectrometer operated at a magnetic field 11.5 tesla are presented. The new instrument utilized an electrostatic ion guide between the ESI source and FTICR trap that provided up to 5% overall transmission efficiency for light ions and up to 30% efficiency for heavier biomolecules. The higher magnetic field in combination with an enlarged FTICR ion trap made it possible to substantially improve resolving power and operate in a more robust fashion for large biopolymers compared to lower field instruments. Mass resolution up to 10
6 has been achieved for intermediate size biopolymers such as bovine ubiquitin (8.6 kDa) and bovine cytochrome
c (12.4 kDa) without the use of frequency drift correction methods. A mass resolution of 370,000 has been demonstrated for isotopically resolved molecular ions of bovine serum albumin (66.5 kDa). Comparative measurements were made with the same spectrometer using a lower field 3.5-tesla magnet allowing the performance gains to be more readily quantified. Further improvements in pumping capacity of the vacuum system and efficiency of ion transmission from the source are expected to lead to further substantial sensitivity gains.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>9879379</pmid><doi>10.1016/S1044-0305(98)00037-3</doi><tpages>9</tpages><orcidid>https://orcid.org/0000000223812349</orcidid><orcidid>https://orcid.org/0000000309366570</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of the American Society for Mass Spectrometry, 1998-07, Vol.9 (7), p.692-700 |
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| language | eng |
| recordid | cdi_proquest_miscellaneous_69125299 |
| source | MEDLINE; Springer Online Journals Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Algorithms Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Biomolecules Biopolymers Cattle Cyclotron resonance Cyclotrons Efficiency Electromagnetic Fields Electrospraying ESI, FTICR, mass spectrometry, tesla, molecular mass range Exact sciences and technology Fourier Analysis Fourier transforms Frequency drift Fundamental and applied biological sciences. Psychology General aspects, investigation methods Instruments, apparatus, components and techniques common to several branches of physics and astronomy Ionization Ions Light ions Magnetic fields Magnetic resonance Mass spectrometers and related techniques Mass spectrometry Mass Spectrometry - instrumentation Mass Spectrometry - methods Molecular ions Physics Proteins Proteins - chemistry Resolution Serum albumin Transmission efficiency |
| title | Electrospray Ionization–Fourier Transform Ion Cyclotron Resonance Mass Spectrometry at 11.5 Tesla: Instrument Design and Initial Results |
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