Peptide-Based In Vitro Assay for the Detection of Reactive Metabolites

We describe a novel peptide-based in vitro method for the detection of reactive metabolites that is amenable for use with microsomal or purified enzyme systems. Covalently bound adducts are detected by mass spectrometry using a surface-enhanced laser desorption ionizationtime of flight detector. Th...

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Veröffentlicht in:	Chemical research in toxicology 2008-04, Vol.21 (4), p.859-868
Hauptverfasser:	Mitchell, Michael D, Elrick, Mollisa M, Walgren, Jennie L, Mueller, Richard A, Morris, Dale L, Thompson, David C
Format:	Artikel
Sprache:	eng
Schlagworte:	Aldehyde Dehydrogenase - metabolism Animals Biological Assay Biotransformation Cytochrome P-450 CYP3A - genetics Cytochrome P-450 CYP3A - metabolism Horseradish Peroxidase - metabolism Humans Microsomes, Liver - metabolism Peptides - metabolism Peroxidase - metabolism Rats Recombinant Proteins - genetics Recombinant Proteins - metabolism
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