Lobeline inhibits Ca2+ current in cultured neurones from rat sympathetic ganglia
The effect of lobeline was studied on the voltage-activated Ca2+ current in sympathetic neurones from the rat superior cervical ganglia using the whole-cell variant of the patch-clamp technique. Lobeline (10-300 microM) inhibited the Ca2+ current evoked by voltage steps from -80 mV (holding potentia...
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| Veröffentlicht in: | European journal of pharmacology 1998-12, Vol.363 (1), p.75-80 |
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| container_title | European journal of pharmacology |
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| creator | TOTH, P. T VIZI, E. S |
| description | The effect of lobeline was studied on the voltage-activated Ca2+ current in sympathetic neurones from the rat superior cervical ganglia using the whole-cell variant of the patch-clamp technique. Lobeline (10-300 microM) inhibited the Ca2+ current evoked by voltage steps from -80 mV (holding potential) to 0 mV (test potential) in a dose dependent manner. The inhibitory effects of noradrenaline (10 microM) and lobeline (100 microM) were compared using a prepulse protocol with high (+80 mV) depolarization. Within the same cell depolarizing prepulses decreased the inhibitory effect of noradrenaline but did not change the extent of lobeline inhibition. Addition of GTPgammaS (300 microM) to the pipette solution did not prevent the inhibitory effect of lobeline (100 microM) but greatly reduced that of noradrenaline (100 microM). Our experiments suggest, that the weak nicotinic agonist lobeline exerts a direct blocking effect on Ca2+ channels at concentrations commonly used to release transmitters. |
| doi_str_mv | 10.1016/S0014-2999(98)00743-2 |
| format | Article |
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Our experiments suggest, that the weak nicotinic agonist lobeline exerts a direct blocking effect on Ca2+ channels at concentrations commonly used to release transmitters.</description><identifier>ISSN: 0014-2999</identifier><identifier>EISSN: 1879-0712</identifier><identifier>DOI: 10.1016/S0014-2999(98)00743-2</identifier><identifier>PMID: 9877084</identifier><identifier>CODEN: EJPHAZ</identifier><language>eng</language><publisher>Amsterdam: Elsevier</publisher><subject>Animals ; Animals, Newborn ; Biological and medical sciences ; Calcium Channel Blockers - pharmacology ; Cells, Cultured ; Cholinergic system ; Dose-Response Relationship, Drug ; Evoked Potentials ; Ganglia, Sympathetic - drug effects ; Lobeline - pharmacology ; Medical sciences ; Neuropharmacology ; Neurotransmitters. Neurotransmission. Receptors ; Norepinephrine - pharmacology ; Patch-Clamp Techniques ; Pharmacology. 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T</creatorcontrib><creatorcontrib>VIZI, E. S</creatorcontrib><title>Lobeline inhibits Ca2+ current in cultured neurones from rat sympathetic ganglia</title><title>European journal of pharmacology</title><addtitle>Eur J Pharmacol</addtitle><description>The effect of lobeline was studied on the voltage-activated Ca2+ current in sympathetic neurones from the rat superior cervical ganglia using the whole-cell variant of the patch-clamp technique. Lobeline (10-300 microM) inhibited the Ca2+ current evoked by voltage steps from -80 mV (holding potential) to 0 mV (test potential) in a dose dependent manner. The inhibitory effects of noradrenaline (10 microM) and lobeline (100 microM) were compared using a prepulse protocol with high (+80 mV) depolarization. Within the same cell depolarizing prepulses decreased the inhibitory effect of noradrenaline but did not change the extent of lobeline inhibition. Addition of GTPgammaS (300 microM) to the pipette solution did not prevent the inhibitory effect of lobeline (100 microM) but greatly reduced that of noradrenaline (100 microM). Our experiments suggest, that the weak nicotinic agonist lobeline exerts a direct blocking effect on Ca2+ channels at concentrations commonly used to release transmitters.</description><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Biological and medical sciences</subject><subject>Calcium Channel Blockers - pharmacology</subject><subject>Cells, Cultured</subject><subject>Cholinergic system</subject><subject>Dose-Response Relationship, Drug</subject><subject>Evoked Potentials</subject><subject>Ganglia, Sympathetic - drug effects</subject><subject>Lobeline - pharmacology</subject><subject>Medical sciences</subject><subject>Neuropharmacology</subject><subject>Neurotransmitters. Neurotransmission. Receptors</subject><subject>Norepinephrine - pharmacology</subject><subject>Patch-Clamp Techniques</subject><subject>Pharmacology. Drug treatments</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Sympathetic Nervous System - drug effects</subject><issn>0014-2999</issn><issn>1879-0712</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkF9LwzAUxYMoc04_wiAPIopUk7RpkkcZ_oOBgvockjTdIm06k_Rh395uK_p0L-eee-7lB8AcozuMcHn_gRAuMiKEuBb8BiFW5Bk5AlPMmcgQw-QYTP8sp-Asxm-EEBWETsBEcMYQL6bgfdlp2zhvofNrp12KcKHILTR9CNanQR3aJvXBVtDbPnTeRliHroVBJRi37UaltU3OwJXyq8apc3BSqybai7HOwNfT4-fiJVu-Pb8uHpaZISVNGSOkwlpTRnHODDdc0Yqy4WvFsM65ruoyp0wRhm3OCVNFXlSDrA2vdM0rms_A1SF3E7qf3sYkWxeNbRrlbddHWQpMUEl2RnowmtDFGGwtN8G1KmwlRnJHUu5Jyh0mKbjck5Rk2JuPB3rd2upva0Q3zC_HuYpGNXVQ3rj4H15SShjPfwEOZntm</recordid><startdate>19981211</startdate><enddate>19981211</enddate><creator>TOTH, P. T</creator><creator>VIZI, E. S</creator><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19981211</creationdate><title>Lobeline inhibits Ca2+ current in cultured neurones from rat sympathetic ganglia</title><author>TOTH, P. T ; VIZI, E. S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c265t-722d1bb575137c8c8a5d57712a71b38bdf6357a271e3827a434d38bbc8dbf8d53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Biological and medical sciences</topic><topic>Calcium Channel Blockers - pharmacology</topic><topic>Cells, Cultured</topic><topic>Cholinergic system</topic><topic>Dose-Response Relationship, Drug</topic><topic>Evoked Potentials</topic><topic>Ganglia, Sympathetic - drug effects</topic><topic>Lobeline - pharmacology</topic><topic>Medical sciences</topic><topic>Neuropharmacology</topic><topic>Neurotransmitters. Neurotransmission. Receptors</topic><topic>Norepinephrine - pharmacology</topic><topic>Patch-Clamp Techniques</topic><topic>Pharmacology. Drug treatments</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Sympathetic Nervous System - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>TOTH, P. T</creatorcontrib><creatorcontrib>VIZI, E. S</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>TOTH, P. T</au><au>VIZI, E. S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lobeline inhibits Ca2+ current in cultured neurones from rat sympathetic ganglia</atitle><jtitle>European journal of pharmacology</jtitle><addtitle>Eur J Pharmacol</addtitle><date>1998-12-11</date><risdate>1998</risdate><volume>363</volume><issue>1</issue><spage>75</spage><epage>80</epage><pages>75-80</pages><issn>0014-2999</issn><eissn>1879-0712</eissn><coden>EJPHAZ</coden><abstract>The effect of lobeline was studied on the voltage-activated Ca2+ current in sympathetic neurones from the rat superior cervical ganglia using the whole-cell variant of the patch-clamp technique. Lobeline (10-300 microM) inhibited the Ca2+ current evoked by voltage steps from -80 mV (holding potential) to 0 mV (test potential) in a dose dependent manner. The inhibitory effects of noradrenaline (10 microM) and lobeline (100 microM) were compared using a prepulse protocol with high (+80 mV) depolarization. Within the same cell depolarizing prepulses decreased the inhibitory effect of noradrenaline but did not change the extent of lobeline inhibition. Addition of GTPgammaS (300 microM) to the pipette solution did not prevent the inhibitory effect of lobeline (100 microM) but greatly reduced that of noradrenaline (100 microM). Our experiments suggest, that the weak nicotinic agonist lobeline exerts a direct blocking effect on Ca2+ channels at concentrations commonly used to release transmitters.</abstract><cop>Amsterdam</cop><pub>Elsevier</pub><pmid>9877084</pmid><doi>10.1016/S0014-2999(98)00743-2</doi><tpages>6</tpages></addata></record> |
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| ispartof | European journal of pharmacology, 1998-12, Vol.363 (1), p.75-80 |
| issn | 0014-2999 1879-0712 |
| language | eng |
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| subjects | Animals Animals, Newborn Biological and medical sciences Calcium Channel Blockers - pharmacology Cells, Cultured Cholinergic system Dose-Response Relationship, Drug Evoked Potentials Ganglia, Sympathetic - drug effects Lobeline - pharmacology Medical sciences Neuropharmacology Neurotransmitters. Neurotransmission. Receptors Norepinephrine - pharmacology Patch-Clamp Techniques Pharmacology. Drug treatments Rats Rats, Wistar Sympathetic Nervous System - drug effects |
| title | Lobeline inhibits Ca2+ current in cultured neurones from rat sympathetic ganglia |
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