Nitric oxide enhances thyroid peroxidase activity in primary human thyrocytes
Recent studies have demonstrated the production of the multi-functional messenger molecule nitric oxide (NO) by the thyroid gland. To examine a possible role for NO in thyroid function, we studied the acute and chronic effect of NO donors on thyroid peroxidase (TPO) activity in monolayer cultures of...
Gespeichert in:
| Veröffentlicht in: | Life sciences (1973) 1998, Vol.63 (26), p.PL373-PL380 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | PL380 |
|---|---|
| container_issue | 26 |
| container_start_page | PL373 |
| container_title | Life sciences (1973) |
| container_volume | 63 |
| creator | Millatt, Lesley J. Johnstone, Alan P. Whitley, Guy StJ |
| description | Recent studies have demonstrated the production of the multi-functional messenger molecule nitric oxide (NO) by the thyroid gland. To examine a possible role for NO in thyroid function, we studied the acute and chronic effect of NO donors on thyroid peroxidase (TPO) activity in monolayer cultures of primary human thyrocytes, using a colorimerric assay technique. The presence of either S-nitrosoglutathione (GS-NO) or sodium nitroprusside (SNP) (10
−6–10
−4 M) at the time of the assay caused a significant increase in TPO activity. Pre-incubation of thyrocytes with 10
.5 M GS-NO for 3 days had no effect on the level of TPO activity when the assay was performed in the absence of NO donors. However, GS-NO pre-incubation significantly enhanced the acute stimulatory effect of GS-NO and SNP on TPO activity. These results suggest a possible role for NO in the regulation of TPO activity and thus thyroid hormone synthesis. |
| doi_str_mv | 10.1016/S0024-3205(98)00527-X |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69120101</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S002432059800527X</els_id><sourcerecordid>69120101</sourcerecordid><originalsourceid>FETCH-LOGICAL-c360t-63afa22cbffeab0880e5f0fecd2441f17d1fd5d6e9ac17e5a1a1c86ea06528783</originalsourceid><addsrcrecordid>eNqFkLtOwzAUhi0EKqXwCJU8IRgCttM4zoRQxU0qMABSN8u1j1WjNim2U5G3J22irkxn-L9z-xAaU3JDCeW3H4SwSZIykl0V4pqQjOXJ_AgNqciLhPCUHqPhATlFZyF8k5bK8nSABoXIc8bEEL2-ueidxtWvM4ChXKpSQ8Bx2fjKGbwBv0tUAKx0dFsXG-xKvPFurXyDl_ValR2smwjhHJ1YtQpw0dcR-np8-Jw-J7P3p5fp_SzRKScx4amyijG9sBbUgghBILPEgjZsMqGW5oZakxkOhdI0h0xRRbXgoAjPmMhFOkKX3dyNr35qCFGuXdCwWqkSqjpIXlBGWkstmHWg9lUIHqzsT5eUyJ1Gudcod45kIeReo5y3feN-Qb1Ygzl09d7a_K7Lof1y68DLoB206ozzoKM0lftnwx9rAYRA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69120101</pqid></control><display><type>article</type><title>Nitric oxide enhances thyroid peroxidase activity in primary human thyrocytes</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Millatt, Lesley J. ; Johnstone, Alan P. ; Whitley, Guy StJ</creator><creatorcontrib>Millatt, Lesley J. ; Johnstone, Alan P. ; Whitley, Guy StJ</creatorcontrib><description>Recent studies have demonstrated the production of the multi-functional messenger molecule nitric oxide (NO) by the thyroid gland. To examine a possible role for NO in thyroid function, we studied the acute and chronic effect of NO donors on thyroid peroxidase (TPO) activity in monolayer cultures of primary human thyrocytes, using a colorimerric assay technique. The presence of either S-nitrosoglutathione (GS-NO) or sodium nitroprusside (SNP) (10
−6–10
−4 M) at the time of the assay caused a significant increase in TPO activity. Pre-incubation of thyrocytes with 10
.5 M GS-NO for 3 days had no effect on the level of TPO activity when the assay was performed in the absence of NO donors. However, GS-NO pre-incubation significantly enhanced the acute stimulatory effect of GS-NO and SNP on TPO activity. These results suggest a possible role for NO in the regulation of TPO activity and thus thyroid hormone synthesis.</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/S0024-3205(98)00527-X</identifier><identifier>PMID: 9877228</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Cells, Cultured ; Glutathione - analogs & derivatives ; Glutathione - pharmacology ; Humans ; Iodide Peroxidase - metabolism ; nitric oxide ; Nitric Oxide - physiology ; Nitric Oxide Donors - pharmacology ; Nitric Oxide Synthase - metabolism ; Nitroprusside - pharmacology ; Nitroso Compounds - pharmacology ; S-Nitrosoglutathione ; thyrocyte ; Thyroid Gland - cytology ; Thyroid Gland - enzymology ; thyroid peroxidase</subject><ispartof>Life sciences (1973), 1998, Vol.63 (26), p.PL373-PL380</ispartof><rights>1998</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c360t-63afa22cbffeab0880e5f0fecd2441f17d1fd5d6e9ac17e5a1a1c86ea06528783</citedby><cites>FETCH-LOGICAL-c360t-63afa22cbffeab0880e5f0fecd2441f17d1fd5d6e9ac17e5a1a1c86ea06528783</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0024-3205(98)00527-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9877228$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Millatt, Lesley J.</creatorcontrib><creatorcontrib>Johnstone, Alan P.</creatorcontrib><creatorcontrib>Whitley, Guy StJ</creatorcontrib><title>Nitric oxide enhances thyroid peroxidase activity in primary human thyrocytes</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description>Recent studies have demonstrated the production of the multi-functional messenger molecule nitric oxide (NO) by the thyroid gland. To examine a possible role for NO in thyroid function, we studied the acute and chronic effect of NO donors on thyroid peroxidase (TPO) activity in monolayer cultures of primary human thyrocytes, using a colorimerric assay technique. The presence of either S-nitrosoglutathione (GS-NO) or sodium nitroprusside (SNP) (10
−6–10
−4 M) at the time of the assay caused a significant increase in TPO activity. Pre-incubation of thyrocytes with 10
.5 M GS-NO for 3 days had no effect on the level of TPO activity when the assay was performed in the absence of NO donors. However, GS-NO pre-incubation significantly enhanced the acute stimulatory effect of GS-NO and SNP on TPO activity. These results suggest a possible role for NO in the regulation of TPO activity and thus thyroid hormone synthesis.</description><subject>Cells, Cultured</subject><subject>Glutathione - analogs & derivatives</subject><subject>Glutathione - pharmacology</subject><subject>Humans</subject><subject>Iodide Peroxidase - metabolism</subject><subject>nitric oxide</subject><subject>Nitric Oxide - physiology</subject><subject>Nitric Oxide Donors - pharmacology</subject><subject>Nitric Oxide Synthase - metabolism</subject><subject>Nitroprusside - pharmacology</subject><subject>Nitroso Compounds - pharmacology</subject><subject>S-Nitrosoglutathione</subject><subject>thyrocyte</subject><subject>Thyroid Gland - cytology</subject><subject>Thyroid Gland - enzymology</subject><subject>thyroid peroxidase</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkLtOwzAUhi0EKqXwCJU8IRgCttM4zoRQxU0qMABSN8u1j1WjNim2U5G3J22irkxn-L9z-xAaU3JDCeW3H4SwSZIykl0V4pqQjOXJ_AgNqciLhPCUHqPhATlFZyF8k5bK8nSABoXIc8bEEL2-ueidxtWvM4ChXKpSQ8Bx2fjKGbwBv0tUAKx0dFsXG-xKvPFurXyDl_ValR2smwjhHJ1YtQpw0dcR-np8-Jw-J7P3p5fp_SzRKScx4amyijG9sBbUgghBILPEgjZsMqGW5oZakxkOhdI0h0xRRbXgoAjPmMhFOkKX3dyNr35qCFGuXdCwWqkSqjpIXlBGWkstmHWg9lUIHqzsT5eUyJ1Gudcod45kIeReo5y3feN-Qb1Ygzl09d7a_K7Lof1y68DLoB206ozzoKM0lftnwx9rAYRA</recordid><startdate>1998</startdate><enddate>1998</enddate><creator>Millatt, Lesley J.</creator><creator>Johnstone, Alan P.</creator><creator>Whitley, Guy StJ</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1998</creationdate><title>Nitric oxide enhances thyroid peroxidase activity in primary human thyrocytes</title><author>Millatt, Lesley J. ; Johnstone, Alan P. ; Whitley, Guy StJ</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-63afa22cbffeab0880e5f0fecd2441f17d1fd5d6e9ac17e5a1a1c86ea06528783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Cells, Cultured</topic><topic>Glutathione - analogs & derivatives</topic><topic>Glutathione - pharmacology</topic><topic>Humans</topic><topic>Iodide Peroxidase - metabolism</topic><topic>nitric oxide</topic><topic>Nitric Oxide - physiology</topic><topic>Nitric Oxide Donors - pharmacology</topic><topic>Nitric Oxide Synthase - metabolism</topic><topic>Nitroprusside - pharmacology</topic><topic>Nitroso Compounds - pharmacology</topic><topic>S-Nitrosoglutathione</topic><topic>thyrocyte</topic><topic>Thyroid Gland - cytology</topic><topic>Thyroid Gland - enzymology</topic><topic>thyroid peroxidase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Millatt, Lesley J.</creatorcontrib><creatorcontrib>Johnstone, Alan P.</creatorcontrib><creatorcontrib>Whitley, Guy StJ</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Millatt, Lesley J.</au><au>Johnstone, Alan P.</au><au>Whitley, Guy StJ</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nitric oxide enhances thyroid peroxidase activity in primary human thyrocytes</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>1998</date><risdate>1998</risdate><volume>63</volume><issue>26</issue><spage>PL373</spage><epage>PL380</epage><pages>PL373-PL380</pages><issn>0024-3205</issn><eissn>1879-0631</eissn><abstract>Recent studies have demonstrated the production of the multi-functional messenger molecule nitric oxide (NO) by the thyroid gland. To examine a possible role for NO in thyroid function, we studied the acute and chronic effect of NO donors on thyroid peroxidase (TPO) activity in monolayer cultures of primary human thyrocytes, using a colorimerric assay technique. The presence of either S-nitrosoglutathione (GS-NO) or sodium nitroprusside (SNP) (10
−6–10
−4 M) at the time of the assay caused a significant increase in TPO activity. Pre-incubation of thyrocytes with 10
.5 M GS-NO for 3 days had no effect on the level of TPO activity when the assay was performed in the absence of NO donors. However, GS-NO pre-incubation significantly enhanced the acute stimulatory effect of GS-NO and SNP on TPO activity. These results suggest a possible role for NO in the regulation of TPO activity and thus thyroid hormone synthesis.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>9877228</pmid><doi>10.1016/S0024-3205(98)00527-X</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0024-3205 |
| ispartof | Life sciences (1973), 1998, Vol.63 (26), p.PL373-PL380 |
| issn | 0024-3205 1879-0631 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69120101 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Cells, Cultured Glutathione - analogs & derivatives Glutathione - pharmacology Humans Iodide Peroxidase - metabolism nitric oxide Nitric Oxide - physiology Nitric Oxide Donors - pharmacology Nitric Oxide Synthase - metabolism Nitroprusside - pharmacology Nitroso Compounds - pharmacology S-Nitrosoglutathione thyrocyte Thyroid Gland - cytology Thyroid Gland - enzymology thyroid peroxidase |
| title | Nitric oxide enhances thyroid peroxidase activity in primary human thyrocytes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T10%3A33%3A40IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Nitric%20oxide%20enhances%20thyroid%20peroxidase%20activity%20in%20primary%20human%20thyrocytes&rft.jtitle=Life%20sciences%20(1973)&rft.au=Millatt,%20Lesley%20J.&rft.date=1998&rft.volume=63&rft.issue=26&rft.spage=PL373&rft.epage=PL380&rft.pages=PL373-PL380&rft.issn=0024-3205&rft.eissn=1879-0631&rft_id=info:doi/10.1016/S0024-3205(98)00527-X&rft_dat=%3Cproquest_cross%3E69120101%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=69120101&rft_id=info:pmid/9877228&rft_els_id=S002432059800527X&rfr_iscdi=true |