Detection of T Lymphocytes in Intestine of Broiler Chicks Treated with Lactobacillus spp. and Challenged with Salmonella enterica Serovar Enteritidis
The expression of immune response in the form of leukocytic infiltrate by CD3+, CD4+, and CD8+ cells in the epithelium and in the intestinal lamina propria of chicks was studied in the present work by means of immunohistochemical reaction. The chicks were treated with Lactobacillus spp. or cecal mic...
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| Veröffentlicht in: | Poultry science 2008-05, Vol.87 (5), p.927-933 |
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| creator | Noujaim, J.C Andreatti Filho, R.L Lima, E.T Okamoto, A.S Amorim, R.L Neto, R. Torres |
| description | The expression of immune response in the form of leukocytic infiltrate by CD3+, CD4+, and CD8+ cells in the epithelium and in the intestinal lamina propria of chicks was studied in the present work by means of immunohistochemical reaction. The chicks were treated with Lactobacillus spp. or cecal microflora (CM) and experimentally challenged or not with Salmonella enterica serovar Enteritidis. The 320 birds utilized were divided into 4 groups containing 80 chicks each and submitted to treatments with Lactobacillus reuteri, Lactobacillus salivarius, Lactobacillus acidophilus, and CM. Each group was subdivided into 4 subgroups of 20 birds each and classified into a subgroup that did not receive treatment (negative control), subgroup treated, subgroup treated and challenged with Salmonella Enteritidis, and subgroup only challenged with Salmonella Enteritidis (positive control). The results obtained show that the treatment with L. reuteri, L. salivarius, L. acidophilus, or CM and challenged or not with Salmonella Enteritidis determine immune response in the form of leukocytic infiltrate by CD3+ and CD8+ lymphocytes followed by CD4+ in the epithelium and in the lamina propria of the duodenum, jejunum, and cecum of chicks up to 12 d of age. The quantity of CD3+ lymphocytes was significantly higher (P < 0.05) in the intestine of chicks treated with L. acidophilus or CM and challenged or not with Salmonella Enteritidis; however, the higher quantity of CD8+ lymphocytes was in the intestine of chicks treated with CM and challenged with Salmonella Enteritidis. The duodenum was the segment in which the immune response by T cells (CD3+, CD4+, and CD8+) was stimulated with the greatest intensity, followed by, respectively, the jejunum and cecum. The quantity of CD3+ lymphocytes present in the duodenum, jejunum, and cecum increases with the age of chicks, independent of the stimulus determined by treatments or challenge. |
| doi_str_mv | 10.3382/ps.2007-00476 |
| format | Article |
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Torres</creator><creatorcontrib>Noujaim, J.C ; Andreatti Filho, R.L ; Lima, E.T ; Okamoto, A.S ; Amorim, R.L ; Neto, R. Torres</creatorcontrib><description>The expression of immune response in the form of leukocytic infiltrate by CD3+, CD4+, and CD8+ cells in the epithelium and in the intestinal lamina propria of chicks was studied in the present work by means of immunohistochemical reaction. The chicks were treated with Lactobacillus spp. or cecal microflora (CM) and experimentally challenged or not with Salmonella enterica serovar Enteritidis. The 320 birds utilized were divided into 4 groups containing 80 chicks each and submitted to treatments with Lactobacillus reuteri, Lactobacillus salivarius, Lactobacillus acidophilus, and CM. Each group was subdivided into 4 subgroups of 20 birds each and classified into a subgroup that did not receive treatment (negative control), subgroup treated, subgroup treated and challenged with Salmonella Enteritidis, and subgroup only challenged with Salmonella Enteritidis (positive control). The results obtained show that the treatment with L. reuteri, L. salivarius, L. acidophilus, or CM and challenged or not with Salmonella Enteritidis determine immune response in the form of leukocytic infiltrate by CD3+ and CD8+ lymphocytes followed by CD4+ in the epithelium and in the lamina propria of the duodenum, jejunum, and cecum of chicks up to 12 d of age. The quantity of CD3+ lymphocytes was significantly higher (P < 0.05) in the intestine of chicks treated with L. acidophilus or CM and challenged or not with Salmonella Enteritidis; however, the higher quantity of CD8+ lymphocytes was in the intestine of chicks treated with CM and challenged with Salmonella Enteritidis. The duodenum was the segment in which the immune response by T cells (CD3+, CD4+, and CD8+) was stimulated with the greatest intensity, followed by, respectively, the jejunum and cecum. The quantity of CD3+ lymphocytes present in the duodenum, jejunum, and cecum increases with the age of chicks, independent of the stimulus determined by treatments or challenge.</description><identifier>ISSN: 0032-5791</identifier><identifier>EISSN: 1525-3171</identifier><identifier>DOI: 10.3382/ps.2007-00476</identifier><identifier>PMID: 18420983</identifier><language>eng</language><publisher>Oxford, UK: Poultry Science Association</publisher><subject>animal age ; Animals ; broiler chickens ; Cecum - microbiology ; cell-mediated immunity ; Chickens ; chicks ; disease models ; immunohistochemistry ; Immunohistochemistry - veterinary ; immunostimulants ; intestinal mucosa ; Intestines - cytology ; Intestines - immunology ; Lactobacillus ; Lactobacillus - immunology ; lymphocyte proliferation ; Probiotics ; protective effect ; Salmonella enteritidis ; Salmonella enteritidis - immunology ; Salmonella Infections, Animal - prevention & control ; salmonellosis ; small intestine ; T-lymphocytes ; T-Lymphocytes - metabolism</subject><ispartof>Poultry science, 2008-05, Vol.87 (5), p.927-933</ispartof><rights>2008 Poultry Science Association, Inc. 2008</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c387t-b14b2e70ce2240358413da30ded1e1169463d6427f656a8580f9355ff8d4ff593</citedby><cites>FETCH-LOGICAL-c387t-b14b2e70ce2240358413da30ded1e1169463d6427f656a8580f9355ff8d4ff593</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18420983$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Noujaim, J.C</creatorcontrib><creatorcontrib>Andreatti Filho, R.L</creatorcontrib><creatorcontrib>Lima, E.T</creatorcontrib><creatorcontrib>Okamoto, A.S</creatorcontrib><creatorcontrib>Amorim, R.L</creatorcontrib><creatorcontrib>Neto, R. Torres</creatorcontrib><title>Detection of T Lymphocytes in Intestine of Broiler Chicks Treated with Lactobacillus spp. and Challenged with Salmonella enterica Serovar Enteritidis</title><title>Poultry science</title><addtitle>Poult Sci</addtitle><description>The expression of immune response in the form of leukocytic infiltrate by CD3+, CD4+, and CD8+ cells in the epithelium and in the intestinal lamina propria of chicks was studied in the present work by means of immunohistochemical reaction. The chicks were treated with Lactobacillus spp. or cecal microflora (CM) and experimentally challenged or not with Salmonella enterica serovar Enteritidis. The 320 birds utilized were divided into 4 groups containing 80 chicks each and submitted to treatments with Lactobacillus reuteri, Lactobacillus salivarius, Lactobacillus acidophilus, and CM. Each group was subdivided into 4 subgroups of 20 birds each and classified into a subgroup that did not receive treatment (negative control), subgroup treated, subgroup treated and challenged with Salmonella Enteritidis, and subgroup only challenged with Salmonella Enteritidis (positive control). The results obtained show that the treatment with L. reuteri, L. salivarius, L. acidophilus, or CM and challenged or not with Salmonella Enteritidis determine immune response in the form of leukocytic infiltrate by CD3+ and CD8+ lymphocytes followed by CD4+ in the epithelium and in the lamina propria of the duodenum, jejunum, and cecum of chicks up to 12 d of age. The quantity of CD3+ lymphocytes was significantly higher (P < 0.05) in the intestine of chicks treated with L. acidophilus or CM and challenged or not with Salmonella Enteritidis; however, the higher quantity of CD8+ lymphocytes was in the intestine of chicks treated with CM and challenged with Salmonella Enteritidis. The duodenum was the segment in which the immune response by T cells (CD3+, CD4+, and CD8+) was stimulated with the greatest intensity, followed by, respectively, the jejunum and cecum. The quantity of CD3+ lymphocytes present in the duodenum, jejunum, and cecum increases with the age of chicks, independent of the stimulus determined by treatments or challenge.</description><subject>animal age</subject><subject>Animals</subject><subject>broiler chickens</subject><subject>Cecum - microbiology</subject><subject>cell-mediated immunity</subject><subject>Chickens</subject><subject>chicks</subject><subject>disease models</subject><subject>immunohistochemistry</subject><subject>Immunohistochemistry - veterinary</subject><subject>immunostimulants</subject><subject>intestinal mucosa</subject><subject>Intestines - cytology</subject><subject>Intestines - immunology</subject><subject>Lactobacillus</subject><subject>Lactobacillus - immunology</subject><subject>lymphocyte proliferation</subject><subject>Probiotics</subject><subject>protective effect</subject><subject>Salmonella enteritidis</subject><subject>Salmonella enteritidis - immunology</subject><subject>Salmonella Infections, Animal - prevention & control</subject><subject>salmonellosis</subject><subject>small intestine</subject><subject>T-lymphocytes</subject><subject>T-Lymphocytes - metabolism</subject><issn>0032-5791</issn><issn>1525-3171</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU1vEzEQhi0EoqFw5Ao-IS4b_LH-2CMNBSpF4pD0bDn2uDHsrhd7F5Qf0v_bTZOKEyePPI8ezcyL0FtKlpxr9mkoS0aIqgiplXyGFlQwUXGq6HO0IISzSqiGXqBXpfwkhFEp1Ut0QXXNSKP5At1_gRHcGFOPU8BbvD50wz65wwgFxx7f9HMxxh6O3aucYgsZr_bR_Sp4m8GO4PHfOO7x2rox7ayLbTsVXIZhiW3vZ9S2LfR3T9jGtl3qoW0thlmdo7N4Azn9sRlfP36M0cfyGr0Iti3w5vxeotuv19vV92r949vN6vO6clyrsdrResdAEQeM1YQLXVPuLScePAVKZVNL7mXNVJBCWi00CQ0XIgTt6xBEwy_Rh5N3yOn3NG9quljccbwe0lSMbChVmqkZrE6gy6mUDMEMOXY2Hwwl5piDGYo55mAec5j5d2fxtOvA_6PPh5-BjycgTcP_XNWT6_0JDTYZe5djMbcbRignRGvJeMMfAE9Gmoo</recordid><startdate>20080501</startdate><enddate>20080501</enddate><creator>Noujaim, J.C</creator><creator>Andreatti Filho, R.L</creator><creator>Lima, E.T</creator><creator>Okamoto, A.S</creator><creator>Amorim, R.L</creator><creator>Neto, R. Torres</creator><general>Poultry Science Association</general><general>Oxford University Press</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080501</creationdate><title>Detection of T Lymphocytes in Intestine of Broiler Chicks Treated with Lactobacillus spp. and Challenged with Salmonella enterica Serovar Enteritidis</title><author>Noujaim, J.C ; Andreatti Filho, R.L ; Lima, E.T ; Okamoto, A.S ; Amorim, R.L ; Neto, R. Torres</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c387t-b14b2e70ce2240358413da30ded1e1169463d6427f656a8580f9355ff8d4ff593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>animal age</topic><topic>Animals</topic><topic>broiler chickens</topic><topic>Cecum - microbiology</topic><topic>cell-mediated immunity</topic><topic>Chickens</topic><topic>chicks</topic><topic>disease models</topic><topic>immunohistochemistry</topic><topic>Immunohistochemistry - veterinary</topic><topic>immunostimulants</topic><topic>intestinal mucosa</topic><topic>Intestines - cytology</topic><topic>Intestines - immunology</topic><topic>Lactobacillus</topic><topic>Lactobacillus - immunology</topic><topic>lymphocyte proliferation</topic><topic>Probiotics</topic><topic>protective effect</topic><topic>Salmonella enteritidis</topic><topic>Salmonella enteritidis - immunology</topic><topic>Salmonella Infections, Animal - prevention & control</topic><topic>salmonellosis</topic><topic>small intestine</topic><topic>T-lymphocytes</topic><topic>T-Lymphocytes - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Noujaim, J.C</creatorcontrib><creatorcontrib>Andreatti Filho, R.L</creatorcontrib><creatorcontrib>Lima, E.T</creatorcontrib><creatorcontrib>Okamoto, A.S</creatorcontrib><creatorcontrib>Amorim, R.L</creatorcontrib><creatorcontrib>Neto, R. Torres</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Poultry science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Noujaim, J.C</au><au>Andreatti Filho, R.L</au><au>Lima, E.T</au><au>Okamoto, A.S</au><au>Amorim, R.L</au><au>Neto, R. Torres</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of T Lymphocytes in Intestine of Broiler Chicks Treated with Lactobacillus spp. and Challenged with Salmonella enterica Serovar Enteritidis</atitle><jtitle>Poultry science</jtitle><addtitle>Poult Sci</addtitle><date>2008-05-01</date><risdate>2008</risdate><volume>87</volume><issue>5</issue><spage>927</spage><epage>933</epage><pages>927-933</pages><issn>0032-5791</issn><eissn>1525-3171</eissn><abstract>The expression of immune response in the form of leukocytic infiltrate by CD3+, CD4+, and CD8+ cells in the epithelium and in the intestinal lamina propria of chicks was studied in the present work by means of immunohistochemical reaction. The chicks were treated with Lactobacillus spp. or cecal microflora (CM) and experimentally challenged or not with Salmonella enterica serovar Enteritidis. The 320 birds utilized were divided into 4 groups containing 80 chicks each and submitted to treatments with Lactobacillus reuteri, Lactobacillus salivarius, Lactobacillus acidophilus, and CM. Each group was subdivided into 4 subgroups of 20 birds each and classified into a subgroup that did not receive treatment (negative control), subgroup treated, subgroup treated and challenged with Salmonella Enteritidis, and subgroup only challenged with Salmonella Enteritidis (positive control). The results obtained show that the treatment with L. reuteri, L. salivarius, L. acidophilus, or CM and challenged or not with Salmonella Enteritidis determine immune response in the form of leukocytic infiltrate by CD3+ and CD8+ lymphocytes followed by CD4+ in the epithelium and in the lamina propria of the duodenum, jejunum, and cecum of chicks up to 12 d of age. The quantity of CD3+ lymphocytes was significantly higher (P < 0.05) in the intestine of chicks treated with L. acidophilus or CM and challenged or not with Salmonella Enteritidis; however, the higher quantity of CD8+ lymphocytes was in the intestine of chicks treated with CM and challenged with Salmonella Enteritidis. The duodenum was the segment in which the immune response by T cells (CD3+, CD4+, and CD8+) was stimulated with the greatest intensity, followed by, respectively, the jejunum and cecum. The quantity of CD3+ lymphocytes present in the duodenum, jejunum, and cecum increases with the age of chicks, independent of the stimulus determined by treatments or challenge.</abstract><cop>Oxford, UK</cop><pub>Poultry Science Association</pub><pmid>18420983</pmid><doi>10.3382/ps.2007-00476</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0032-5791 |
| ispartof | Poultry science, 2008-05, Vol.87 (5), p.927-933 |
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| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | animal age Animals broiler chickens Cecum - microbiology cell-mediated immunity Chickens chicks disease models immunohistochemistry Immunohistochemistry - veterinary immunostimulants intestinal mucosa Intestines - cytology Intestines - immunology Lactobacillus Lactobacillus - immunology lymphocyte proliferation Probiotics protective effect Salmonella enteritidis Salmonella enteritidis - immunology Salmonella Infections, Animal - prevention & control salmonellosis small intestine T-lymphocytes T-Lymphocytes - metabolism |
| title | Detection of T Lymphocytes in Intestine of Broiler Chicks Treated with Lactobacillus spp. and Challenged with Salmonella enterica Serovar Enteritidis |
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