Deconvolution by omission libraries

Omission libraries, synthesized by omitting one amino acid in all coupling positions, are very efficient tools for the rapid identification of the amino acid components of bioactive peptides. Based on the determined amino acids, an occurrence library can be defined and prepared which is much less complex than the full one while still comprising the bioactive peptide. Omission peptide libraries can be prepared by omitting the same (one) amino acid from all coupling positions. An omission library can be used to determine whether or not the omitted amino acid is present in the sequence of the bioactive peptide. It is shown that by testing the whole set of omission libraries (one for each amino acid) the amino acid composition of the bioactive peptide can be determined.