Use of fortuitous in vitro mutations in a synthetic Cu-Zn superoxide dismutase gene to illuminate protein structure-function relationships

A completely synthetic bovine copper-zinc superoxide dismutase gene (Cu-ZnSOD), designed using the most favoured codons for expression in yeast, was constructed. Fortuitous mutations introduced while cloning the synthetic gene permitted the additional construction of four altered-polypeptide product...

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Veröffentlicht in:	Protein engineering 1998-11, Vol.11 (11), p.1083-1088
Hauptverfasser:	Kanan, J H, Hubbard, S J, Minter, S J, Oliver, S G
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Base Sequence Cattle Cloning, Molecular DNA, Recombinant Molecular Sequence Data Mutation Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - metabolism Superoxide Dismutase - chemistry Superoxide Dismutase - genetics Superoxide Dismutase - metabolism
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