Nitric oxide synthase from bovine pancreas: Purification and characterization

Nitric oxide synthase, NOS (EC.1.14.13.39), was purified from bovine pancreas over 5,500-fold with a 7.6% yield using 30% ammonium sulfate precipitation, and 2′,5′-ADP-agarose and calmodulin-agarose affinity chromatography. The purified bovine pancreatic NOS (bpNOS) showed a single band on SDS-PAGE...

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Veröffentlicht in:	Archives of pharmacal research 1998-04, Vol.21 (2), p.128-134
Hauptverfasser:	Nam, Suk Woo, Seo, Dong Wan, Sung, Dae Seok, Han, Jeung Whan, Hong, Sung Youl, Lee, Hyang Woo
Format:	Artikel
Sprache:	eng
Schlagworte:	acinar cells affinity chromatography ammonium sulfate Animals antibodies arginine Blotting, Western Bovines brain calcium calmodulin Cattle Chromatography, Gel citrulline Electrophoresis, Polyacrylamide Gel enzyme activity Immunohistochemistry Molecular Weight NADP (coenzyme) nitric oxide synthase Nitric Oxide Synthase - analysis Nitric Oxide Synthase - isolation & purification Nitric Oxide Synthase - metabolism pancreas Pancreas - enzymology polyacrylamide gel electrophoresis Proteins - analysis Proteins - isolation & purification
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