Expression of genes encoding the E2 and E3 components of the Bacillus stearothermophilus pyruvate dehydrogenase complex and the stoichiometry of subunit interaction in assembly in vitro

Genes encoding the dihydrolipoyl acetyltransferase (E2) and dihydrolipoyl dehydrogenase (E3) components of the pyruvate dehydrogenase (PDH) multienzyme complex from Bacillus stearothermophilus were overexpressed in Escherichia coli. The E2 component was purified as a large soluble aggregate (molecul...

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Veröffentlicht in:	European journal of biochemistry 1998-12, Vol.258 (2), p.491-501
Hauptverfasser:	Lessard, Ivan A. D., Domingo, Gonzalo J., Borges, Adolfo, Perham, Richard N.
Format:	Artikel
Sprache:	eng
Schlagworte:	AE2 gene AE3 gene Bacillus stearothermophilus dihydrolipoyl acetyltransferase dihydrolipoyl dehydrogenase gene expression Genes, Bacterial - genetics Geobacillus stearothermophilus - enzymology Guanidine - pharmacology Microscopy, Electron Peptide Synthases - metabolism post-translational modification protein assembly Protein Binding - genetics Protein Conformation Protein Denaturation Protein Folding Protein Processing, Post-Translational - genetics pyruvate dehydrogenase complex Pyruvate Dehydrogenase Complex - chemistry Pyruvate Dehydrogenase Complex - genetics Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - ultrastructure Thioctic Acid - metabolism
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