Biosensor for on-line measurement of bovine progesterone during milking
Reproductive management is a major financial concern of the dairy industry, with missed estrus detection a main cause of lost income. A biosensor was developed for on-line measurement of progesterone in bovine milk and detection of estrus. The biosensor used an enzyme immunoassay format for molecula...
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Veröffentlicht in: | Biosensors & bioelectronics 1998-11, Vol.13 (11), p.1173-1180 |
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creator | Claycomb, Rodney W. Delwiche, Michael J. |
description | Reproductive management is a major financial concern of the dairy industry, with missed estrus detection a main cause of lost income. A biosensor was developed for on-line measurement of progesterone in bovine milk and detection of estrus. The biosensor used an enzyme immunoassay format for molecular recognition, which was developed to run in approximately eight minutes. The sensor was designed to operate on-line in a dairy parlor using microinjection pumps and valves for fluid transport, fiber optics and photodiodes for light measurement, and a control computer for sequencing. Calibration showed a dynamic response between 0.1 and 5
ng/ml progesterone in milk. The reusability of the test well was evaluated. Thiocyanate (0.5
M, pH
5.1) quickly regenerated the antibody surface while maintaining antibody activity for 15–20
cycles, but noise from the residual enzyme limited reusability. |
doi_str_mv | 10.1016/S0956-5663(98)00080-3 |
format | Article |
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ng/ml progesterone in milk. The reusability of the test well was evaluated. Thiocyanate (0.5
M, pH
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ng/ml progesterone in milk. The reusability of the test well was evaluated. Thiocyanate (0.5
M, pH
5.1) quickly regenerated the antibody surface while maintaining antibody activity for 15–20
cycles, but noise from the residual enzyme limited reusability.</description><subject>Animal productions</subject><subject>Animals</subject><subject>antibody</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Calibration</subject><subject>Cattle</subject><subject>cow</subject><subject>Dairy products</subject><subject>estrus</subject><subject>Fiber optics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>hormone</subject><subject>Immunoenzyme Techniques</subject><subject>Light measurement</subject><subject>Methods. Procedures. Technologies</subject><subject>Milk - chemistry</subject><subject>Photodiodes</subject><subject>Progesterone - analysis</subject><subject>Terrestrial animal productions</subject><subject>Various methods and equipments</subject><subject>Vertebrates</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1PGzEQhq2qCNLQn4C0B1S1hwV_f5wQRG2KFIkDcLa83lnkdncd7CwS_74OidJjDtbYnmdmXs2L0AXBVwQTef2IjZC1kJJ9N_oHxljjmn1CM6IVqzll4jOaHZAz9CXnPwVSxOBTdGp0uSg6Q8u7EDOMOaaqKyeOdR9GqAZweUowwLipYlc18W37u07xBfIGUiyPdkphfKmG0P8t8RyddK7P8HUf5-j518-nxe969bC8X9yuas8F2dRc8YYpzIiXBFQjW6GlYMxzo4rklnlNJWNK-c5wEE3rWkclBUqpV8Z5zebo265v0fI6FTF2CNlD37sR4pStNIRwKs1RkCrOBWbqOEiYVlxuR4sd6FPMOUFn1ykMLr1bgu3WEvthid3u2xptPyyxrNRd7AdMzQDtoWrvQclf7vMue9d3yY0-5P_NFcFSiILd7DAo630LkGz2AUYPbUjgN7aN4YiQf7Wwpt4</recordid><startdate>19981115</startdate><enddate>19981115</enddate><creator>Claycomb, Rodney W.</creator><creator>Delwiche, Michael J.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SP</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>19981115</creationdate><title>Biosensor for on-line measurement of bovine progesterone during milking</title><author>Claycomb, Rodney W. ; Delwiche, Michael J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451t-474b37031c61e7b6d586533c497423d3c8263377cf94e5bdada262e222c79ac83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animal productions</topic><topic>Animals</topic><topic>antibody</topic><topic>Biological and medical sciences</topic><topic>Biosensing Techniques</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Calibration</topic><topic>Cattle</topic><topic>cow</topic><topic>Dairy products</topic><topic>estrus</topic><topic>Fiber optics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>hormone</topic><topic>Immunoenzyme Techniques</topic><topic>Light measurement</topic><topic>Methods. Procedures. Technologies</topic><topic>Milk - chemistry</topic><topic>Photodiodes</topic><topic>Progesterone - analysis</topic><topic>Terrestrial animal productions</topic><topic>Various methods and equipments</topic><topic>Vertebrates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Claycomb, Rodney W.</creatorcontrib><creatorcontrib>Delwiche, Michael J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Claycomb, Rodney W.</au><au>Delwiche, Michael J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biosensor for on-line measurement of bovine progesterone during milking</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>1998-11-15</date><risdate>1998</risdate><volume>13</volume><issue>11</issue><spage>1173</spage><epage>1180</epage><pages>1173-1180</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>Reproductive management is a major financial concern of the dairy industry, with missed estrus detection a main cause of lost income. A biosensor was developed for on-line measurement of progesterone in bovine milk and detection of estrus. The biosensor used an enzyme immunoassay format for molecular recognition, which was developed to run in approximately eight minutes. The sensor was designed to operate on-line in a dairy parlor using microinjection pumps and valves for fluid transport, fiber optics and photodiodes for light measurement, and a control computer for sequencing. Calibration showed a dynamic response between 0.1 and 5
ng/ml progesterone in milk. The reusability of the test well was evaluated. Thiocyanate (0.5
M, pH
5.1) quickly regenerated the antibody surface while maintaining antibody activity for 15–20
cycles, but noise from the residual enzyme limited reusability.</abstract><cop>Lausanne</cop><pub>Elsevier B.V</pub><pmid>9871972</pmid><doi>10.1016/S0956-5663(98)00080-3</doi><tpages>8</tpages></addata></record> |
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source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
subjects | Animal productions Animals antibody Biological and medical sciences Biosensing Techniques Biosensors Biotechnology Calibration Cattle cow Dairy products estrus Fiber optics Fundamental and applied biological sciences. Psychology hormone Immunoenzyme Techniques Light measurement Methods. Procedures. Technologies Milk - chemistry Photodiodes Progesterone - analysis Terrestrial animal productions Various methods and equipments Vertebrates |
title | Biosensor for on-line measurement of bovine progesterone during milking |
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