Biosensor for on-line measurement of bovine progesterone during milking

Reproductive management is a major financial concern of the dairy industry, with missed estrus detection a main cause of lost income. A biosensor was developed for on-line measurement of progesterone in bovine milk and detection of estrus. The biosensor used an enzyme immunoassay format for molecula...

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Veröffentlicht in:Biosensors & bioelectronics 1998-11, Vol.13 (11), p.1173-1180
Hauptverfasser: Claycomb, Rodney W., Delwiche, Michael J.
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container_issue 11
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container_title Biosensors & bioelectronics
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creator Claycomb, Rodney W.
Delwiche, Michael J.
description Reproductive management is a major financial concern of the dairy industry, with missed estrus detection a main cause of lost income. A biosensor was developed for on-line measurement of progesterone in bovine milk and detection of estrus. The biosensor used an enzyme immunoassay format for molecular recognition, which was developed to run in approximately eight minutes. The sensor was designed to operate on-line in a dairy parlor using microinjection pumps and valves for fluid transport, fiber optics and photodiodes for light measurement, and a control computer for sequencing. Calibration showed a dynamic response between 0.1 and 5 ng/ml progesterone in milk. The reusability of the test well was evaluated. Thiocyanate (0.5 M, pH 5.1) quickly regenerated the antibody surface while maintaining antibody activity for 15–20 cycles, but noise from the residual enzyme limited reusability.
doi_str_mv 10.1016/S0956-5663(98)00080-3
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source MEDLINE; ScienceDirect Journals (5 years ago - present)
subjects Animal productions
Animals
antibody
Biological and medical sciences
Biosensing Techniques
Biosensors
Biotechnology
Calibration
Cattle
cow
Dairy products
estrus
Fiber optics
Fundamental and applied biological sciences. Psychology
hormone
Immunoenzyme Techniques
Light measurement
Methods. Procedures. Technologies
Milk - chemistry
Photodiodes
Progesterone - analysis
Terrestrial animal productions
Various methods and equipments
Vertebrates
title Biosensor for on-line measurement of bovine progesterone during milking
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