$Combination of affinity depletion of abundant proteins and reversed-phase fractionation in proteomic analysis of human plasma/serum$

Combination of affinity depletion of abundant proteins and reversed-phase fractionation in proteomic analysis of human plasma/serum

The tremendous complexity of the serum and plasma proteome presents extreme analytical challenges in comprehensive analysis due to the wide dynamic range of protein concentrations. Therefore, robust sample preparation methods remain one of the important steps in the proteome characterization workflo...

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Veröffentlicht in:	Journal of Chromatography A 2008-05, Vol.1189 (1), p.332-338
Hauptverfasser:	Zolotarjova, Nina, Mrozinski, Peter, Chen, Haiying, Martosella, James
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Biological and medical sciences Blood Proteins - analysis Blood Proteins - chemistry Blood Proteins - isolation & purification Chemical Fractionation Chromatography, High Pressure Liquid - methods Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology General aspects, investigation methods Human plasma Human serum Humans Immunoaffinity depletion Protein depletion Protein fractionation Proteins Proteome - analysis Proteome - chemistry Proteome - isolation & purification Proteomics Proteomics - methods Tandem Mass Spectrometry
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