Structural and functional characterization of myotoxin, Cr-IV 1, a phospholipase A2 D49 from the venom of the snake Calloselasma rhodostoma

A new D49 PLA(2) was purified from the venom of Calloselasma rhodostoma after two chromatographic steps. Molecular exclusion chromatography was done through a Protein-Pack 300 SW column (0.78 cm x 30 cm), eluting with 0.25 M ammonium bicarbonate, pH 7.9, at a flow rate of 0.3 ml/min. Reverse-phase H...

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Veröffentlicht in:	Biologicals 2008-05, Vol.36 (3), p.168-176
Hauptverfasser:	Bonfim, V L, Ponce-Soto, L A, Martins de Souza, D, Souza, G H M F, Baldasso, P A, Eberlin, M N, Marangoni, S
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Biochemistry - methods Chromatography, High Pressure Liquid Crotalid Venoms - chemistry Crotalid Venoms - metabolism Electrophoresis, Polyacrylamide Gel Hydrogen-Ion Concentration Mice Models, Biological Molecular Sequence Data Muscle, Skeletal - metabolism Phospholipases A2 - chemistry Phospholipases A2 - metabolism Sequence Homology, Amino Acid Snakes Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Online-Zugang:	Volltext
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