Saikosaponin b2 Induces Differentiation without Growth Inhibition in Cultured B16 Melanoma Cells
Treatment with 5 μM of saikosaponin (SS) b2 for 30 days was found to induce differentiation of B16 melanoma cells, with potentiation of expressions of melanogenesis and tyrosinase. To explore the mechanism of this effect, we observed the cell growth, cell cycle and morphology, and found that SSb2 di...
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| Veröffentlicht in: | Cell Structure and Function 1998, Vol.23(5), pp.265-272 |
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| container_title | Cell Structure and Function |
| container_volume | 23 |
| creator | Zong, Zhi-ping Fujikawa-Yamamoto, Kohzaburo Ota, Takahide Guan, Xin Murakami, Manabu Li, Ai-li Yamaguchi, Nobuo Tanino, Mikio Odashima, Shizuo |
| description | Treatment with 5 μM of saikosaponin (SS) b2 for 30 days was found to induce differentiation of B16 melanoma cells, with potentiation of expressions of melanogenesis and tyrosinase. To explore the mechanism of this effect, we observed the cell growth, cell cycle and morphology, and found that SSb2 did not affect any of these parameters. That is, SSb2 induced the differentiation of B16 melanoma cells without growth inhibition or cytotoxicity under conditions of low dose and long-term treatment. Phorbol ester, a protein kinase C (PKC) activator, markedly inhibited the expressions of melanogenesis and tyrosinase in both the control B16 melanoma cells and the long-term treated B16 melanoma cells. Down-regulation of the PKC activity may be involved in the effects of SSb2. |
| doi_str_mv | 10.1247/csf.23.265 |
| format | Article |
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To explore the mechanism of this effect, we observed the cell growth, cell cycle and morphology, and found that SSb2 did not affect any of these parameters. That is, SSb2 induced the differentiation of B16 melanoma cells without growth inhibition or cytotoxicity under conditions of low dose and long-term treatment. Phorbol ester, a protein kinase C (PKC) activator, markedly inhibited the expressions of melanogenesis and tyrosinase in both the control B16 melanoma cells and the long-term treated B16 melanoma cells. Down-regulation of the PKC activity may be involved in the effects of SSb2.</description><identifier>ISSN: 0386-7196</identifier><identifier>EISSN: 1347-3700</identifier><identifier>DOI: 10.1247/csf.23.265</identifier><identifier>PMID: 9872567</identifier><language>eng</language><publisher>Japan: Japan Society for Cell Biology</publisher><subject>Animals ; Cell Cycle - drug effects ; Cell Differentiation - drug effects ; Cell Size - drug effects ; differentiation ; Fluorescent Antibody Technique ; Melanins - biosynthesis ; melanoma ; Melanoma, Experimental - mortality ; Melanoma, Experimental - pathology ; Mice ; Monophenol Monooxygenase - metabolism ; Neoplasm Transplantation ; Oleanolic Acid - analogs & derivatives ; PKC ; saikosaponin ; Sapogenins - chemistry ; Sapogenins - pharmacology ; Saponins ; Tumor Cells, Cultured</subject><ispartof>Cell Structure and Function, 1998, Vol.23(5), pp.265-272</ispartof><rights>Japan Society for Cell Biology</rights><rights>Copyright Japan Science and Technology Agency 1998</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4695-17d2ee2fc4b275ab59377fe9c0d401b6f262c889bb3b5132f6439918694c041b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9872567$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zong, Zhi-ping</creatorcontrib><creatorcontrib>Fujikawa-Yamamoto, Kohzaburo</creatorcontrib><creatorcontrib>Ota, Takahide</creatorcontrib><creatorcontrib>Guan, Xin</creatorcontrib><creatorcontrib>Murakami, Manabu</creatorcontrib><creatorcontrib>Li, Ai-li</creatorcontrib><creatorcontrib>Yamaguchi, Nobuo</creatorcontrib><creatorcontrib>Tanino, Mikio</creatorcontrib><creatorcontrib>Odashima, Shizuo</creatorcontrib><title>Saikosaponin b2 Induces Differentiation without Growth Inhibition in Cultured B16 Melanoma Cells</title><title>Cell Structure and Function</title><addtitle>Cell Struct. Funct.</addtitle><description>Treatment with 5 μM of saikosaponin (SS) b2 for 30 days was found to induce differentiation of B16 melanoma cells, with potentiation of expressions of melanogenesis and tyrosinase. To explore the mechanism of this effect, we observed the cell growth, cell cycle and morphology, and found that SSb2 did not affect any of these parameters. That is, SSb2 induced the differentiation of B16 melanoma cells without growth inhibition or cytotoxicity under conditions of low dose and long-term treatment. Phorbol ester, a protein kinase C (PKC) activator, markedly inhibited the expressions of melanogenesis and tyrosinase in both the control B16 melanoma cells and the long-term treated B16 melanoma cells. Down-regulation of the PKC activity may be involved in the effects of SSb2.</description><subject>Animals</subject><subject>Cell Cycle - drug effects</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Size - drug effects</subject><subject>differentiation</subject><subject>Fluorescent Antibody Technique</subject><subject>Melanins - biosynthesis</subject><subject>melanoma</subject><subject>Melanoma, Experimental - mortality</subject><subject>Melanoma, Experimental - pathology</subject><subject>Mice</subject><subject>Monophenol Monooxygenase - metabolism</subject><subject>Neoplasm Transplantation</subject><subject>Oleanolic Acid - analogs & derivatives</subject><subject>PKC</subject><subject>saikosaponin</subject><subject>Sapogenins - chemistry</subject><subject>Sapogenins - pharmacology</subject><subject>Saponins</subject><subject>Tumor Cells, Cultured</subject><issn>0386-7196</issn><issn>1347-3700</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkM9rFDEYhoModa1evAsDQg_CbPM7k6NOtRZaPKjnmGQSN-tssiYZiv-9WXet4OX7Ds_zvXy8ALxEcI0wFZe2-DUma8zZI7BChIqeCAgfgxUkA-8FkvwpeFbKFkLMIBdn4EwOAjMuVuDbZx1-pKL3KYbYGdzdxGmxrnRXwXuXXaxB15Bidx_qJi21u87pvm6atgkm_CHtblzmumQ3de8Q7-7crGPa6W5081yegydez8W9OO1z8PXD-y_jx_720_XN-Pa2t5RL1iMxYeewt9RgwbRhkgjhnbRwohAZ7jHHdhikMcQwRLDnlEiJBi6phRQZcg4ujrn7nH4urlS1C8W2D3R0aSmKS4SggKiJr_8Tt2nJsf2mEGWS0YEJ3Kw3R8vmVEp2Xu1z2On8SyGoDqWrVrrCRLXSm_zqFLmYnZse1FPLjY9Hvi1Vf3cPXOca7OwOUUgKdoj7Ozj7Rzc6KxfJbxjwk2k</recordid><startdate>1998</startdate><enddate>1998</enddate><creator>Zong, Zhi-ping</creator><creator>Fujikawa-Yamamoto, Kohzaburo</creator><creator>Ota, Takahide</creator><creator>Guan, Xin</creator><creator>Murakami, Manabu</creator><creator>Li, Ai-li</creator><creator>Yamaguchi, Nobuo</creator><creator>Tanino, Mikio</creator><creator>Odashima, Shizuo</creator><general>Japan Society for Cell Biology</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>1998</creationdate><title>Saikosaponin b2 Induces Differentiation without Growth Inhibition in Cultured B16 Melanoma Cells</title><author>Zong, Zhi-ping ; Fujikawa-Yamamoto, Kohzaburo ; Ota, Takahide ; Guan, Xin ; Murakami, Manabu ; Li, Ai-li ; Yamaguchi, Nobuo ; Tanino, Mikio ; Odashima, Shizuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4695-17d2ee2fc4b275ab59377fe9c0d401b6f262c889bb3b5132f6439918694c041b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Cell Cycle - drug effects</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Size - drug effects</topic><topic>differentiation</topic><topic>Fluorescent Antibody Technique</topic><topic>Melanins - biosynthesis</topic><topic>melanoma</topic><topic>Melanoma, Experimental - mortality</topic><topic>Melanoma, Experimental - pathology</topic><topic>Mice</topic><topic>Monophenol Monooxygenase - metabolism</topic><topic>Neoplasm Transplantation</topic><topic>Oleanolic Acid - analogs & derivatives</topic><topic>PKC</topic><topic>saikosaponin</topic><topic>Sapogenins - chemistry</topic><topic>Sapogenins - pharmacology</topic><topic>Saponins</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zong, Zhi-ping</creatorcontrib><creatorcontrib>Fujikawa-Yamamoto, Kohzaburo</creatorcontrib><creatorcontrib>Ota, Takahide</creatorcontrib><creatorcontrib>Guan, Xin</creatorcontrib><creatorcontrib>Murakami, Manabu</creatorcontrib><creatorcontrib>Li, Ai-li</creatorcontrib><creatorcontrib>Yamaguchi, Nobuo</creatorcontrib><creatorcontrib>Tanino, Mikio</creatorcontrib><creatorcontrib>Odashima, Shizuo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell Structure and Function</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zong, Zhi-ping</au><au>Fujikawa-Yamamoto, Kohzaburo</au><au>Ota, Takahide</au><au>Guan, Xin</au><au>Murakami, Manabu</au><au>Li, Ai-li</au><au>Yamaguchi, Nobuo</au><au>Tanino, Mikio</au><au>Odashima, Shizuo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Saikosaponin b2 Induces Differentiation without Growth Inhibition in Cultured B16 Melanoma Cells</atitle><jtitle>Cell Structure and Function</jtitle><addtitle>Cell Struct. 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| source | J-STAGE Free; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Cell Cycle - drug effects Cell Differentiation - drug effects Cell Size - drug effects differentiation Fluorescent Antibody Technique Melanins - biosynthesis melanoma Melanoma, Experimental - mortality Melanoma, Experimental - pathology Mice Monophenol Monooxygenase - metabolism Neoplasm Transplantation Oleanolic Acid - analogs & derivatives PKC saikosaponin Sapogenins - chemistry Sapogenins - pharmacology Saponins Tumor Cells, Cultured |
| title | Saikosaponin b2 Induces Differentiation without Growth Inhibition in Cultured B16 Melanoma Cells |
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