Exhaled Breath Condensate Isoprostanes Are Elevated in Patients With Acute Lung Injury or ARDS
Oxidant stress is a purported mechanism of tissue damage in patients with ARDS and acute lung injury (ALI). Isoprostanes, prostanoid compounds primarily formed nonenzymatically via lipid peroxidation, are precise markers of in vivo oxidant stress. Plasma levels of metabolites of 8-iso-prostaglandin-...
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| Veröffentlicht in: | Chest 1998-12, Vol.114 (6), p.1653-1659 |
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| creator | Carpenter, Chace T. Price, Patricia V. Christman, Brian W. |
| description | Oxidant stress is a purported mechanism of tissue damage in patients with ARDS and acute lung injury (ALI). Isoprostanes, prostanoid compounds primarily formed nonenzymatically via lipid peroxidation, are precise markers of in vivo oxidant stress. Plasma levels of metabolites of 8-iso-prostaglandin-F2α (8-iso-PGF2α) correlate with outcome in patients with ARDS.
To examine exhaled breath condensate levels of 8-iso-PGF2α as a noninvasive quantification of pulmonary oxidant stress in patients with, or at risk for, ARDS/ALI.
Breath condensate was collected from 22 patients with, or at risk for, ARDS/ALI by placing Tygon tubing submerged in an ice bath in line with the expiratory limb of the ventilator circuit. Ten patients without lung disease, who were intubated while undergoing minor surgical procedures, served as control subjects. Between 1 and 3 mL of condensate was collected over a 30- to 60-min period, then immediately frozen and stored at −70°C until analysis. The 8-iso-PGF2α was purified and derivatized, then quantified by stable isotope dilution in conjunction with gas chromatography/mass spectrometry.
The mean level of exhaled 8-iso-PGF2α in the patients with ALI/ARDS, 87 ± 28 pg/mL, was significantly higher than the mean in the normal group, 7 ± 4 pg/mL (p = 0.007). The 8-iso-PGF2α levels were greater than two standard deviations above the mean of the normal group in 12 of 22 patients with or at risk for ARDS/ALI.
These results provide further evidence that lipid peroxidation does occur in patients with ARDS/ALI. The measurement of exhaled isoprostanes provides a novel, noninvasive method to quantify oxidant stress in such patients. |
| doi_str_mv | 10.1378/chest.114.6.1653 |
| format | Article |
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To examine exhaled breath condensate levels of 8-iso-PGF2α as a noninvasive quantification of pulmonary oxidant stress in patients with, or at risk for, ARDS/ALI.
Breath condensate was collected from 22 patients with, or at risk for, ARDS/ALI by placing Tygon tubing submerged in an ice bath in line with the expiratory limb of the ventilator circuit. Ten patients without lung disease, who were intubated while undergoing minor surgical procedures, served as control subjects. Between 1 and 3 mL of condensate was collected over a 30- to 60-min period, then immediately frozen and stored at −70°C until analysis. The 8-iso-PGF2α was purified and derivatized, then quantified by stable isotope dilution in conjunction with gas chromatography/mass spectrometry.
The mean level of exhaled 8-iso-PGF2α in the patients with ALI/ARDS, 87 ± 28 pg/mL, was significantly higher than the mean in the normal group, 7 ± 4 pg/mL (p = 0.007). The 8-iso-PGF2α levels were greater than two standard deviations above the mean of the normal group in 12 of 22 patients with or at risk for ARDS/ALI.
These results provide further evidence that lipid peroxidation does occur in patients with ARDS/ALI. The measurement of exhaled isoprostanes provides a novel, noninvasive method to quantify oxidant stress in such patients.</description><identifier>ISSN: 0012-3692</identifier><identifier>EISSN: 1931-3543</identifier><identifier>DOI: 10.1378/chest.114.6.1653</identifier><identifier>PMID: 9872202</identifier><identifier>CODEN: CHETBF</identifier><language>eng</language><publisher>Northbrook, IL: Elsevier Inc</publisher><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ; ARDS ; Biological and medical sciences ; breath condensate ; Breath Tests ; Dinoprost - analogs & derivatives ; Dinoprost - analysis ; Dinoprost - urine ; Emergency and intensive respiratory care ; F2-Isoprostanes ; Female ; Heart rate ; Humans ; Intensive care medicine ; isoprostanes ; Lipid Peroxidation ; Lipids ; lung injury ; Male ; Medical sciences ; Middle Aged ; Neutrophils ; oxidant stress ; Oxidative Stress ; prostaglandins ; Pulmonary arteries ; Respiratory Distress Syndrome, Adult - metabolism ; Vasoconstrictor Agents - analysis ; Vasoconstrictor Agents - urine ; Ventilation ; Ventilators</subject><ispartof>Chest, 1998-12, Vol.114 (6), p.1653-1659</ispartof><rights>1998 The American College of Chest Physicians</rights><rights>1999 INIST-CNRS</rights><rights>Copyright American College of Chest Physicians Dec 1998</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-302475a6dcf21d5e38efee0eb420f75973a9a6d19aeb5eb49d4c0cfa3fe4ee213</citedby><cites>FETCH-LOGICAL-c442t-302475a6dcf21d5e38efee0eb420f75973a9a6d19aeb5eb49d4c0cfa3fe4ee213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1624001$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9872202$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Carpenter, Chace T.</creatorcontrib><creatorcontrib>Price, Patricia V.</creatorcontrib><creatorcontrib>Christman, Brian W.</creatorcontrib><title>Exhaled Breath Condensate Isoprostanes Are Elevated in Patients With Acute Lung Injury or ARDS</title><title>Chest</title><addtitle>Chest</addtitle><description>Oxidant stress is a purported mechanism of tissue damage in patients with ARDS and acute lung injury (ALI). Isoprostanes, prostanoid compounds primarily formed nonenzymatically via lipid peroxidation, are precise markers of in vivo oxidant stress. Plasma levels of metabolites of 8-iso-prostaglandin-F2α (8-iso-PGF2α) correlate with outcome in patients with ARDS.
To examine exhaled breath condensate levels of 8-iso-PGF2α as a noninvasive quantification of pulmonary oxidant stress in patients with, or at risk for, ARDS/ALI.
Breath condensate was collected from 22 patients with, or at risk for, ARDS/ALI by placing Tygon tubing submerged in an ice bath in line with the expiratory limb of the ventilator circuit. Ten patients without lung disease, who were intubated while undergoing minor surgical procedures, served as control subjects. Between 1 and 3 mL of condensate was collected over a 30- to 60-min period, then immediately frozen and stored at −70°C until analysis. The 8-iso-PGF2α was purified and derivatized, then quantified by stable isotope dilution in conjunction with gas chromatography/mass spectrometry.
The mean level of exhaled 8-iso-PGF2α in the patients with ALI/ARDS, 87 ± 28 pg/mL, was significantly higher than the mean in the normal group, 7 ± 4 pg/mL (p = 0.007). The 8-iso-PGF2α levels were greater than two standard deviations above the mean of the normal group in 12 of 22 patients with or at risk for ARDS/ALI.
These results provide further evidence that lipid peroxidation does occur in patients with ARDS/ALI. The measurement of exhaled isoprostanes provides a novel, noninvasive method to quantify oxidant stress in such patients.</description><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>ARDS</subject><subject>Biological and medical sciences</subject><subject>breath condensate</subject><subject>Breath Tests</subject><subject>Dinoprost - analogs & derivatives</subject><subject>Dinoprost - analysis</subject><subject>Dinoprost - urine</subject><subject>Emergency and intensive respiratory care</subject><subject>F2-Isoprostanes</subject><subject>Female</subject><subject>Heart rate</subject><subject>Humans</subject><subject>Intensive care medicine</subject><subject>isoprostanes</subject><subject>Lipid Peroxidation</subject><subject>Lipids</subject><subject>lung injury</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Neutrophils</subject><subject>oxidant stress</subject><subject>Oxidative Stress</subject><subject>prostaglandins</subject><subject>Pulmonary arteries</subject><subject>Respiratory Distress Syndrome, Adult - metabolism</subject><subject>Vasoconstrictor Agents - analysis</subject><subject>Vasoconstrictor Agents - urine</subject><subject>Ventilation</subject><subject>Ventilators</subject><issn>0012-3692</issn><issn>1931-3543</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1UcuO0zAUtRBo6Azs2SBZCM0uxa8kNbtSClSqBOIhdliufTNxlTqDnQzM33M7jUAgsbLs87jnHhPyhLM5l_XihWshD3PO1bya86qU98iMa8kLWSp5n8wY46KQlRYPyXnOe4Z3rqszcqYXtRBMzMi39c_WduDpqwR2aOmqjx5itgPQTe6vU58HGyHTZQK67uAGAU9DpB_sECAOmX4NqFq6EQXbMV7RTdyP6Zb2iS4_vv70iDxobJfh8XRekC9v1p9X74rt-7eb1XJbOKXEUEgmVF3ayrtGcF-CXEADwGCnBGvqUtfSakS5trAr8VV75ZhrrGxAAQguL8jlyRcTfx-xE3MI2UHXYfh-zKbSnC1KdiQ--4e478cUMZsRjCnOVKWQxE4kh_vnBI25TuFg063hzBx7N3e9G-zdVObYO0qeTr7j7gD-t2AqGvHnE26zs12TbHQh__GthGJ38abJbbhqf4QEJh9s16GpPM2c0v41-eVJAtjvTYBkssOvceBR7gbj-_D_2L8AD4uweg</recordid><startdate>19981201</startdate><enddate>19981201</enddate><creator>Carpenter, Chace T.</creator><creator>Price, Patricia V.</creator><creator>Christman, Brian W.</creator><general>Elsevier Inc</general><general>American College of Chest Physicians</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>19981201</creationdate><title>Exhaled Breath Condensate Isoprostanes Are Elevated in Patients With Acute Lung Injury or ARDS</title><author>Carpenter, Chace T. ; Price, Patricia V. ; Christman, Brian W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-302475a6dcf21d5e38efee0eb420f75973a9a6d19aeb5eb49d4c0cfa3fe4ee213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</topic><topic>ARDS</topic><topic>Biological and medical sciences</topic><topic>breath condensate</topic><topic>Breath Tests</topic><topic>Dinoprost - analogs & derivatives</topic><topic>Dinoprost - analysis</topic><topic>Dinoprost - urine</topic><topic>Emergency and intensive respiratory care</topic><topic>F2-Isoprostanes</topic><topic>Female</topic><topic>Heart rate</topic><topic>Humans</topic><topic>Intensive care medicine</topic><topic>isoprostanes</topic><topic>Lipid Peroxidation</topic><topic>Lipids</topic><topic>lung injury</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Neutrophils</topic><topic>oxidant stress</topic><topic>Oxidative Stress</topic><topic>prostaglandins</topic><topic>Pulmonary arteries</topic><topic>Respiratory Distress Syndrome, Adult - metabolism</topic><topic>Vasoconstrictor Agents - analysis</topic><topic>Vasoconstrictor Agents - urine</topic><topic>Ventilation</topic><topic>Ventilators</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Carpenter, Chace T.</creatorcontrib><creatorcontrib>Price, Patricia V.</creatorcontrib><creatorcontrib>Christman, Brian W.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Chest</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Carpenter, Chace T.</au><au>Price, Patricia V.</au><au>Christman, Brian W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Exhaled Breath Condensate Isoprostanes Are Elevated in Patients With Acute Lung Injury or ARDS</atitle><jtitle>Chest</jtitle><addtitle>Chest</addtitle><date>1998-12-01</date><risdate>1998</risdate><volume>114</volume><issue>6</issue><spage>1653</spage><epage>1659</epage><pages>1653-1659</pages><issn>0012-3692</issn><eissn>1931-3543</eissn><coden>CHETBF</coden><abstract>Oxidant stress is a purported mechanism of tissue damage in patients with ARDS and acute lung injury (ALI). Isoprostanes, prostanoid compounds primarily formed nonenzymatically via lipid peroxidation, are precise markers of in vivo oxidant stress. Plasma levels of metabolites of 8-iso-prostaglandin-F2α (8-iso-PGF2α) correlate with outcome in patients with ARDS.
To examine exhaled breath condensate levels of 8-iso-PGF2α as a noninvasive quantification of pulmonary oxidant stress in patients with, or at risk for, ARDS/ALI.
Breath condensate was collected from 22 patients with, or at risk for, ARDS/ALI by placing Tygon tubing submerged in an ice bath in line with the expiratory limb of the ventilator circuit. Ten patients without lung disease, who were intubated while undergoing minor surgical procedures, served as control subjects. Between 1 and 3 mL of condensate was collected over a 30- to 60-min period, then immediately frozen and stored at −70°C until analysis. The 8-iso-PGF2α was purified and derivatized, then quantified by stable isotope dilution in conjunction with gas chromatography/mass spectrometry.
The mean level of exhaled 8-iso-PGF2α in the patients with ALI/ARDS, 87 ± 28 pg/mL, was significantly higher than the mean in the normal group, 7 ± 4 pg/mL (p = 0.007). The 8-iso-PGF2α levels were greater than two standard deviations above the mean of the normal group in 12 of 22 patients with or at risk for ARDS/ALI.
These results provide further evidence that lipid peroxidation does occur in patients with ARDS/ALI. The measurement of exhaled isoprostanes provides a novel, noninvasive method to quantify oxidant stress in such patients.</abstract><cop>Northbrook, IL</cop><pub>Elsevier Inc</pub><pmid>9872202</pmid><doi>10.1378/chest.114.6.1653</doi><tpages>7</tpages></addata></record> |
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| ispartof | Chest, 1998-12, Vol.114 (6), p.1653-1659 |
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| subjects | Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ARDS Biological and medical sciences breath condensate Breath Tests Dinoprost - analogs & derivatives Dinoprost - analysis Dinoprost - urine Emergency and intensive respiratory care F2-Isoprostanes Female Heart rate Humans Intensive care medicine isoprostanes Lipid Peroxidation Lipids lung injury Male Medical sciences Middle Aged Neutrophils oxidant stress Oxidative Stress prostaglandins Pulmonary arteries Respiratory Distress Syndrome, Adult - metabolism Vasoconstrictor Agents - analysis Vasoconstrictor Agents - urine Ventilation Ventilators |
| title | Exhaled Breath Condensate Isoprostanes Are Elevated in Patients With Acute Lung Injury or ARDS |
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