Isolated Tumor Cells and Circulating CK20 mRNA in pN0 Colorectal Cancer Patients
Micrometastases in lymph nodes and blood may provide important prognostic information. In this study, cytokeratin 20 (CK20) positive cells in lymph nodes and circulating CK20 mRNA were studied using 57 paraffin-embedded lymph node specimens and blood from 24 patients with pN0 colorectal cancer (CRC)...
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| Veröffentlicht in: | International journal of surgical pathology 2008-04, Vol.16 (2), p.119-126 |
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| container_title | International journal of surgical pathology |
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| creator | Wong, Cesar Sze-Chuen Cheung, Mong-Tong Ma, Brigette Buig-Yue Pun Hui, Edwin Chan, Alex Chak-Lam Chan, Chi-Kin Lee, King-Chung Cheuk, Wah Lam, Money Yan-Yee Wong, Manson Chi-Keung Chan, Charles Ming-Lok Chan, John Kwok-Cheung Chan, Anthony Tak-Cheung |
| description | Micrometastases in lymph nodes and blood may provide important prognostic information. In this study, cytokeratin 20 (CK20) positive cells in lymph nodes and circulating CK20 mRNA were studied using 57 paraffin-embedded lymph node specimens and blood from 24 patients with pN0 colorectal cancer (CRC), respectively. Results showed that 29 out of 56 (52%) lymph node specimens had CK20-positive cells (range: 1-35). Follow-up of the patients for 12 months indicated that 4 patients (7%) had CRC metastases to liver, lung, and bone. In addition, 8 out of 24 (33%) samples had at least 2-fold circulating CK20 mRNA expression higher than the pooled normal sample. This study provides evidence that CK20-positive cells were found in the lymph nodes and differentially expressed circulating CK20 mRNA was also detected in the blood from patients with pN0 CRC. Long-term follow-up is necessary to study their prognostic use in patients with non-metastatic CRC. |
| doi_str_mv | 10.1177/1066896907311901 |
| format | Article |
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In this study, cytokeratin 20 (CK20) positive cells in lymph nodes and circulating CK20 mRNA were studied using 57 paraffin-embedded lymph node specimens and blood from 24 patients with pN0 colorectal cancer (CRC), respectively. Results showed that 29 out of 56 (52%) lymph node specimens had CK20-positive cells (range: 1-35). Follow-up of the patients for 12 months indicated that 4 patients (7%) had CRC metastases to liver, lung, and bone. In addition, 8 out of 24 (33%) samples had at least 2-fold circulating CK20 mRNA expression higher than the pooled normal sample. This study provides evidence that CK20-positive cells were found in the lymph nodes and differentially expressed circulating CK20 mRNA was also detected in the blood from patients with pN0 CRC. Long-term follow-up is necessary to study their prognostic use in patients with non-metastatic CRC.</description><identifier>ISSN: 1066-8969</identifier><identifier>EISSN: 1940-2465</identifier><identifier>DOI: 10.1177/1066896907311901</identifier><identifier>PMID: 18387990</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Adenocarcinoma - genetics ; Adenocarcinoma - secondary ; Adult ; Aged ; Aged, 80 and over ; Biomarkers, Tumor - genetics ; Biomarkers, Tumor - metabolism ; Cell Count ; Colorectal Neoplasms - genetics ; Colorectal Neoplasms - pathology ; Female ; Humans ; Immunohistochemistry ; Keratin-20 - genetics ; Keratin-20 - metabolism ; Lymph Nodes - metabolism ; Lymph Nodes - pathology ; Lymphatic Metastasis ; Male ; Middle Aged ; Neoplastic Cells, Circulating - metabolism ; RNA, Messenger - analysis ; RNA, Neoplasm - analysis</subject><ispartof>International journal of surgical pathology, 2008-04, Vol.16 (2), p.119-126</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c366t-ca88cd5c90644d7933b661b040a594819c071c02fb33185e94769a30526ac8913</citedby><cites>FETCH-LOGICAL-c366t-ca88cd5c90644d7933b661b040a594819c071c02fb33185e94769a30526ac8913</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/1066896907311901$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/1066896907311901$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,776,780,21799,27903,27904,43600,43601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18387990$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wong, Cesar Sze-Chuen</creatorcontrib><creatorcontrib>Cheung, Mong-Tong</creatorcontrib><creatorcontrib>Ma, Brigette Buig-Yue</creatorcontrib><creatorcontrib>Pun Hui, Edwin</creatorcontrib><creatorcontrib>Chan, Alex Chak-Lam</creatorcontrib><creatorcontrib>Chan, Chi-Kin</creatorcontrib><creatorcontrib>Lee, King-Chung</creatorcontrib><creatorcontrib>Cheuk, Wah</creatorcontrib><creatorcontrib>Lam, Money Yan-Yee</creatorcontrib><creatorcontrib>Wong, Manson Chi-Keung</creatorcontrib><creatorcontrib>Chan, Charles Ming-Lok</creatorcontrib><creatorcontrib>Chan, John Kwok-Cheung</creatorcontrib><creatorcontrib>Chan, Anthony Tak-Cheung</creatorcontrib><title>Isolated Tumor Cells and Circulating CK20 mRNA in pN0 Colorectal Cancer Patients</title><title>International journal of surgical pathology</title><addtitle>Int J Surg Pathol</addtitle><description>Micrometastases in lymph nodes and blood may provide important prognostic information. In this study, cytokeratin 20 (CK20) positive cells in lymph nodes and circulating CK20 mRNA were studied using 57 paraffin-embedded lymph node specimens and blood from 24 patients with pN0 colorectal cancer (CRC), respectively. Results showed that 29 out of 56 (52%) lymph node specimens had CK20-positive cells (range: 1-35). Follow-up of the patients for 12 months indicated that 4 patients (7%) had CRC metastases to liver, lung, and bone. In addition, 8 out of 24 (33%) samples had at least 2-fold circulating CK20 mRNA expression higher than the pooled normal sample. This study provides evidence that CK20-positive cells were found in the lymph nodes and differentially expressed circulating CK20 mRNA was also detected in the blood from patients with pN0 CRC. Long-term follow-up is necessary to study their prognostic use in patients with non-metastatic CRC.</description><subject>Adenocarcinoma - genetics</subject><subject>Adenocarcinoma - secondary</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Cell Count</subject><subject>Colorectal Neoplasms - genetics</subject><subject>Colorectal Neoplasms - pathology</subject><subject>Female</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Keratin-20 - genetics</subject><subject>Keratin-20 - metabolism</subject><subject>Lymph Nodes - metabolism</subject><subject>Lymph Nodes - pathology</subject><subject>Lymphatic Metastasis</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Neoplastic Cells, Circulating - metabolism</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Neoplasm - analysis</subject><issn>1066-8969</issn><issn>1940-2465</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LAzEQhoMoVqt3T5KTt9WZJJtNjmXxo1hqkXpe0mxatuxHTXYP_ntTWhAE8TQD7zMvw0PIDcI9YpY9IEiptNSQcUQNeEIuUAtImJDpadxjnOzzEbkMYQsATDI8JyNUXGVawwVZTENXm96VdDk0nae5q-tATVvSvPJ2iFHVbmj-yoA27_MJrVq6mwPNu7rzzvamprlprfN0EUnX9uGKnK1NHdz1cY7Jx9PjMn9JZm_P03wySyyXsk-sUcqWqdUghSgzzflKSlyBAJNqoVBbyNACW684R5U6LTKpDYeUSWOVRj4md4fene8-Bxf6oqmCjd-b1nVDKKTG2MDlvyBDkWrFRAThAFrfheDdutj5qjH-q0Ao9rqL37rjye2xe1g1rvw5OPqNQHIAgtm4YtsNvo1S_i78BpoTg6o</recordid><startdate>200804</startdate><enddate>200804</enddate><creator>Wong, Cesar Sze-Chuen</creator><creator>Cheung, Mong-Tong</creator><creator>Ma, Brigette Buig-Yue</creator><creator>Pun Hui, Edwin</creator><creator>Chan, Alex Chak-Lam</creator><creator>Chan, Chi-Kin</creator><creator>Lee, King-Chung</creator><creator>Cheuk, Wah</creator><creator>Lam, Money Yan-Yee</creator><creator>Wong, Manson Chi-Keung</creator><creator>Chan, Charles Ming-Lok</creator><creator>Chan, John Kwok-Cheung</creator><creator>Chan, Anthony Tak-Cheung</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>200804</creationdate><title>Isolated Tumor Cells and Circulating CK20 mRNA in pN0 Colorectal Cancer Patients</title><author>Wong, Cesar Sze-Chuen ; Cheung, Mong-Tong ; Ma, Brigette Buig-Yue ; Pun Hui, Edwin ; Chan, Alex Chak-Lam ; Chan, Chi-Kin ; Lee, King-Chung ; Cheuk, Wah ; Lam, Money Yan-Yee ; Wong, Manson Chi-Keung ; Chan, Charles Ming-Lok ; Chan, John Kwok-Cheung ; Chan, Anthony Tak-Cheung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c366t-ca88cd5c90644d7933b661b040a594819c071c02fb33185e94769a30526ac8913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Adenocarcinoma - genetics</topic><topic>Adenocarcinoma - secondary</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Cell Count</topic><topic>Colorectal Neoplasms - genetics</topic><topic>Colorectal Neoplasms - pathology</topic><topic>Female</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Keratin-20 - genetics</topic><topic>Keratin-20 - metabolism</topic><topic>Lymph Nodes - metabolism</topic><topic>Lymph Nodes - pathology</topic><topic>Lymphatic Metastasis</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Neoplastic Cells, Circulating - metabolism</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Neoplasm - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wong, Cesar Sze-Chuen</creatorcontrib><creatorcontrib>Cheung, Mong-Tong</creatorcontrib><creatorcontrib>Ma, Brigette Buig-Yue</creatorcontrib><creatorcontrib>Pun Hui, Edwin</creatorcontrib><creatorcontrib>Chan, Alex Chak-Lam</creatorcontrib><creatorcontrib>Chan, Chi-Kin</creatorcontrib><creatorcontrib>Lee, King-Chung</creatorcontrib><creatorcontrib>Cheuk, Wah</creatorcontrib><creatorcontrib>Lam, Money Yan-Yee</creatorcontrib><creatorcontrib>Wong, Manson Chi-Keung</creatorcontrib><creatorcontrib>Chan, Charles Ming-Lok</creatorcontrib><creatorcontrib>Chan, John Kwok-Cheung</creatorcontrib><creatorcontrib>Chan, Anthony Tak-Cheung</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of surgical pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wong, Cesar Sze-Chuen</au><au>Cheung, Mong-Tong</au><au>Ma, Brigette Buig-Yue</au><au>Pun Hui, Edwin</au><au>Chan, Alex Chak-Lam</au><au>Chan, Chi-Kin</au><au>Lee, King-Chung</au><au>Cheuk, Wah</au><au>Lam, Money Yan-Yee</au><au>Wong, Manson Chi-Keung</au><au>Chan, Charles Ming-Lok</au><au>Chan, John Kwok-Cheung</au><au>Chan, Anthony Tak-Cheung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolated Tumor Cells and Circulating CK20 mRNA in pN0 Colorectal Cancer Patients</atitle><jtitle>International journal of surgical pathology</jtitle><addtitle>Int J Surg Pathol</addtitle><date>2008-04</date><risdate>2008</risdate><volume>16</volume><issue>2</issue><spage>119</spage><epage>126</epage><pages>119-126</pages><issn>1066-8969</issn><eissn>1940-2465</eissn><abstract>Micrometastases in lymph nodes and blood may provide important prognostic information. In this study, cytokeratin 20 (CK20) positive cells in lymph nodes and circulating CK20 mRNA were studied using 57 paraffin-embedded lymph node specimens and blood from 24 patients with pN0 colorectal cancer (CRC), respectively. Results showed that 29 out of 56 (52%) lymph node specimens had CK20-positive cells (range: 1-35). Follow-up of the patients for 12 months indicated that 4 patients (7%) had CRC metastases to liver, lung, and bone. In addition, 8 out of 24 (33%) samples had at least 2-fold circulating CK20 mRNA expression higher than the pooled normal sample. This study provides evidence that CK20-positive cells were found in the lymph nodes and differentially expressed circulating CK20 mRNA was also detected in the blood from patients with pN0 CRC. Long-term follow-up is necessary to study their prognostic use in patients with non-metastatic CRC.</abstract><cop>Los Angeles, CA</cop><pub>SAGE Publications</pub><pmid>18387990</pmid><doi>10.1177/1066896907311901</doi><tpages>8</tpages></addata></record> |
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| ispartof | International journal of surgical pathology, 2008-04, Vol.16 (2), p.119-126 |
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| subjects | Adenocarcinoma - genetics Adenocarcinoma - secondary Adult Aged Aged, 80 and over Biomarkers, Tumor - genetics Biomarkers, Tumor - metabolism Cell Count Colorectal Neoplasms - genetics Colorectal Neoplasms - pathology Female Humans Immunohistochemistry Keratin-20 - genetics Keratin-20 - metabolism Lymph Nodes - metabolism Lymph Nodes - pathology Lymphatic Metastasis Male Middle Aged Neoplastic Cells, Circulating - metabolism RNA, Messenger - analysis RNA, Neoplasm - analysis |
| title | Isolated Tumor Cells and Circulating CK20 mRNA in pN0 Colorectal Cancer Patients |
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