Cytokine profiles of aqueous humor and graft in orthotopic mouse corneal transplantation
Cytokine profile is a key in understanding the mechanisms of allograft rejection. Cytokine expression in the aqueous humor and the correlation between the aqueous humor cells and corneal infiltrating cells are not fully understood in corneal transplantation. Orthotopic mouse corneal transplantation...
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| Veröffentlicht in: | Transplantation 1998-12, Vol.66 (11), p.1504-1510 |
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| creator | YAMAGAMI, S KAWASHIMA, H ENDO, H TSURU, T SHIBUI, H KAGAWA, Y HORI, J YAMAGAMI, H ISOBE, M |
| description | Cytokine profile is a key in understanding the mechanisms of allograft rejection. Cytokine expression in the aqueous humor and the correlation between the aqueous humor cells and corneal infiltrating cells are not fully understood in corneal transplantation.
Orthotopic mouse corneal transplantation was performed using BALB/c (H2d) mice as recipients, and C3H/He (H2k) and BALB/c mice as donors for allografts and isografts, respectively. Immunocytochemistry was performed on aqueous humor cells. Corneal graft was studied immunohistochemically. Cytokine gene expressions of the cells infiltrating the aqueous humor and corneal grafts were determined by the semiquantitative reverse transcription and polymerase chain reaction method.
Interferon-gamma, interleukin (IL)-2, IL-4, and IL-10 were detected in the cells infiltrating the aqueous humor and corneal grafts at both the protein and gene expression levels. T helper 1 (Th1) cytokine expressions at the protein level, however, were consistently predominant in the rejected allografts compared to those of Th2 cytokines. The cytokine and surface marker profiles of the cells in the aqueous humor corresponded well to those of the cells infiltrating the corneal grafts. Cytokine protein and mRNA expression levels in the aqueous humor decreased rapidly.
Allorejection in corneal transplantation is Th1 cytokine-predominant. Infiltrating cells do not express Th2 cytokine so much in allograft rejection, as compared with Th1 cytokine. The cell infiltration patterns of the aqueous humor were well correlated with those of the cornea. |
| doi_str_mv | 10.1097/00007890-199812150-00014 |
| format | Article |
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Orthotopic mouse corneal transplantation was performed using BALB/c (H2d) mice as recipients, and C3H/He (H2k) and BALB/c mice as donors for allografts and isografts, respectively. Immunocytochemistry was performed on aqueous humor cells. Corneal graft was studied immunohistochemically. Cytokine gene expressions of the cells infiltrating the aqueous humor and corneal grafts were determined by the semiquantitative reverse transcription and polymerase chain reaction method.
Interferon-gamma, interleukin (IL)-2, IL-4, and IL-10 were detected in the cells infiltrating the aqueous humor and corneal grafts at both the protein and gene expression levels. T helper 1 (Th1) cytokine expressions at the protein level, however, were consistently predominant in the rejected allografts compared to those of Th2 cytokines. The cytokine and surface marker profiles of the cells in the aqueous humor corresponded well to those of the cells infiltrating the corneal grafts. Cytokine protein and mRNA expression levels in the aqueous humor decreased rapidly.
Allorejection in corneal transplantation is Th1 cytokine-predominant. Infiltrating cells do not express Th2 cytokine so much in allograft rejection, as compared with Th1 cytokine. The cell infiltration patterns of the aqueous humor were well correlated with those of the cornea.</description><identifier>ISSN: 0041-1337</identifier><identifier>EISSN: 1534-6080</identifier><identifier>DOI: 10.1097/00007890-199812150-00014</identifier><identifier>PMID: 9869092</identifier><identifier>CODEN: TRPLAU</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott</publisher><subject>Animals ; Aqueous Humor - chemistry ; Aqueous Humor - cytology ; Biological and medical sciences ; Cornea - chemistry ; Cornea - metabolism ; Corneal Transplantation - immunology ; Corneal Transplantation - pathology ; Cytokines - genetics ; Gene Expression ; Graft Rejection - genetics ; Immunohistochemistry ; Interferon-gamma - genetics ; Interleukin-10 - genetics ; Interleukin-2 - genetics ; Interleukin-4 - genetics ; Male ; Medical sciences ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C3H ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - metabolism ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Surgery of the eye and orbit</subject><ispartof>Transplantation, 1998-12, Vol.66 (11), p.1504-1510</ispartof><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1616765$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9869092$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>YAMAGAMI, S</creatorcontrib><creatorcontrib>KAWASHIMA, H</creatorcontrib><creatorcontrib>ENDO, H</creatorcontrib><creatorcontrib>TSURU, T</creatorcontrib><creatorcontrib>SHIBUI, H</creatorcontrib><creatorcontrib>KAGAWA, Y</creatorcontrib><creatorcontrib>HORI, J</creatorcontrib><creatorcontrib>YAMAGAMI, H</creatorcontrib><creatorcontrib>ISOBE, M</creatorcontrib><title>Cytokine profiles of aqueous humor and graft in orthotopic mouse corneal transplantation</title><title>Transplantation</title><addtitle>Transplantation</addtitle><description>Cytokine profile is a key in understanding the mechanisms of allograft rejection. Cytokine expression in the aqueous humor and the correlation between the aqueous humor cells and corneal infiltrating cells are not fully understood in corneal transplantation.
Orthotopic mouse corneal transplantation was performed using BALB/c (H2d) mice as recipients, and C3H/He (H2k) and BALB/c mice as donors for allografts and isografts, respectively. Immunocytochemistry was performed on aqueous humor cells. Corneal graft was studied immunohistochemically. Cytokine gene expressions of the cells infiltrating the aqueous humor and corneal grafts were determined by the semiquantitative reverse transcription and polymerase chain reaction method.
Interferon-gamma, interleukin (IL)-2, IL-4, and IL-10 were detected in the cells infiltrating the aqueous humor and corneal grafts at both the protein and gene expression levels. T helper 1 (Th1) cytokine expressions at the protein level, however, were consistently predominant in the rejected allografts compared to those of Th2 cytokines. The cytokine and surface marker profiles of the cells in the aqueous humor corresponded well to those of the cells infiltrating the corneal grafts. Cytokine protein and mRNA expression levels in the aqueous humor decreased rapidly.
Allorejection in corneal transplantation is Th1 cytokine-predominant. Infiltrating cells do not express Th2 cytokine so much in allograft rejection, as compared with Th1 cytokine. The cell infiltration patterns of the aqueous humor were well correlated with those of the cornea.</description><subject>Animals</subject><subject>Aqueous Humor - chemistry</subject><subject>Aqueous Humor - cytology</subject><subject>Biological and medical sciences</subject><subject>Cornea - chemistry</subject><subject>Cornea - metabolism</subject><subject>Corneal Transplantation - immunology</subject><subject>Corneal Transplantation - pathology</subject><subject>Cytokines - genetics</subject><subject>Gene Expression</subject><subject>Graft Rejection - genetics</subject><subject>Immunohistochemistry</subject><subject>Interferon-gamma - genetics</subject><subject>Interleukin-10 - genetics</subject><subject>Interleukin-2 - genetics</subject><subject>Interleukin-4 - genetics</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred C3H</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Surgery of the eye and orbit</subject><issn>0041-1337</issn><issn>1534-6080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LxDAQhoMo67r6E4QcxFs106T5OMriFyx4UfBWpm3qVtumJunBf2_E4tW5DLzzMPO-QwgFdgXMqGuWSmnDMjBGQw4Fy5IC4oCsoeAik0yzQ7JmTEAGnKtjchLCe0IKrtSKrIyWhpl8TV63X9F9dKOlk3dt19tAXUvxc7ZuDnQ_D85THBv65rGNtBup83Hvopu6mg4JsbR2frTY0-hxDFOPY8TYufGUHLXYB3u29A15ubt93j5ku6f7x-3NLptyKWMmsOXKSMSKgy4KNIwzxY1QuWiFrliu8hqSWqu8kcZYCVYqFJrpSjSVRb4hl797k__kOsRy6EJt-2TkJ0IpDTAplfwXBAXSpL8l8HwB52qwTTn5bkD_VS4_S_OLZY6hxr5Nuesu_GEgIZ0r-DeEmXyF</recordid><startdate>19981215</startdate><enddate>19981215</enddate><creator>YAMAGAMI, S</creator><creator>KAWASHIMA, H</creator><creator>ENDO, H</creator><creator>TSURU, T</creator><creator>SHIBUI, H</creator><creator>KAGAWA, Y</creator><creator>HORI, J</creator><creator>YAMAGAMI, H</creator><creator>ISOBE, M</creator><general>Lippincott</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19981215</creationdate><title>Cytokine profiles of aqueous humor and graft in orthotopic mouse corneal transplantation</title><author>YAMAGAMI, S ; KAWASHIMA, H ; ENDO, H ; TSURU, T ; SHIBUI, H ; KAGAWA, Y ; HORI, J ; YAMAGAMI, H ; ISOBE, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p266t-4af3796aab31855a90307394724f48b0272c1a90c72d699e61e67a4808b4dbea3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Aqueous Humor - chemistry</topic><topic>Aqueous Humor - cytology</topic><topic>Biological and medical sciences</topic><topic>Cornea - chemistry</topic><topic>Cornea - metabolism</topic><topic>Corneal Transplantation - immunology</topic><topic>Corneal Transplantation - pathology</topic><topic>Cytokines - genetics</topic><topic>Gene Expression</topic><topic>Graft Rejection - genetics</topic><topic>Immunohistochemistry</topic><topic>Interferon-gamma - genetics</topic><topic>Interleukin-10 - genetics</topic><topic>Interleukin-2 - genetics</topic><topic>Interleukin-4 - genetics</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Inbred C3H</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Surgery of the eye and orbit</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>YAMAGAMI, S</creatorcontrib><creatorcontrib>KAWASHIMA, H</creatorcontrib><creatorcontrib>ENDO, H</creatorcontrib><creatorcontrib>TSURU, T</creatorcontrib><creatorcontrib>SHIBUI, H</creatorcontrib><creatorcontrib>KAGAWA, Y</creatorcontrib><creatorcontrib>HORI, J</creatorcontrib><creatorcontrib>YAMAGAMI, H</creatorcontrib><creatorcontrib>ISOBE, M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Transplantation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>YAMAGAMI, S</au><au>KAWASHIMA, H</au><au>ENDO, H</au><au>TSURU, T</au><au>SHIBUI, H</au><au>KAGAWA, Y</au><au>HORI, J</au><au>YAMAGAMI, H</au><au>ISOBE, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytokine profiles of aqueous humor and graft in orthotopic mouse corneal transplantation</atitle><jtitle>Transplantation</jtitle><addtitle>Transplantation</addtitle><date>1998-12-15</date><risdate>1998</risdate><volume>66</volume><issue>11</issue><spage>1504</spage><epage>1510</epage><pages>1504-1510</pages><issn>0041-1337</issn><eissn>1534-6080</eissn><coden>TRPLAU</coden><abstract>Cytokine profile is a key in understanding the mechanisms of allograft rejection. Cytokine expression in the aqueous humor and the correlation between the aqueous humor cells and corneal infiltrating cells are not fully understood in corneal transplantation.
Orthotopic mouse corneal transplantation was performed using BALB/c (H2d) mice as recipients, and C3H/He (H2k) and BALB/c mice as donors for allografts and isografts, respectively. Immunocytochemistry was performed on aqueous humor cells. Corneal graft was studied immunohistochemically. Cytokine gene expressions of the cells infiltrating the aqueous humor and corneal grafts were determined by the semiquantitative reverse transcription and polymerase chain reaction method.
Interferon-gamma, interleukin (IL)-2, IL-4, and IL-10 were detected in the cells infiltrating the aqueous humor and corneal grafts at both the protein and gene expression levels. T helper 1 (Th1) cytokine expressions at the protein level, however, were consistently predominant in the rejected allografts compared to those of Th2 cytokines. The cytokine and surface marker profiles of the cells in the aqueous humor corresponded well to those of the cells infiltrating the corneal grafts. Cytokine protein and mRNA expression levels in the aqueous humor decreased rapidly.
Allorejection in corneal transplantation is Th1 cytokine-predominant. Infiltrating cells do not express Th2 cytokine so much in allograft rejection, as compared with Th1 cytokine. The cell infiltration patterns of the aqueous humor were well correlated with those of the cornea.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott</pub><pmid>9869092</pmid><doi>10.1097/00007890-199812150-00014</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Aqueous Humor - chemistry Aqueous Humor - cytology Biological and medical sciences Cornea - chemistry Cornea - metabolism Corneal Transplantation - immunology Corneal Transplantation - pathology Cytokines - genetics Gene Expression Graft Rejection - genetics Immunohistochemistry Interferon-gamma - genetics Interleukin-10 - genetics Interleukin-2 - genetics Interleukin-4 - genetics Male Medical sciences Mice Mice, Inbred BALB C Mice, Inbred C3H Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Surgery of the eye and orbit |
| title | Cytokine profiles of aqueous humor and graft in orthotopic mouse corneal transplantation |
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