Spatiotemporal Expression of PSD-95 and nNOS After Rat Sciatic Nerve Injury
Neuronal nitric oxide synthase (nNOS) has been implicated to influence peripheral nerve lesion and regeneration. Post-synaptic density-95 (PSD-95) is one of nNOS-anchoring proteins and plays an important role in specifying the sites of reaction of NO in nervous system. Here we established a rat scia...
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| Veröffentlicht in: | Neurochemical research 2008-06, Vol.33 (6), p.1090-1100 |
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| creator | Gao, Shangfeng Fei, Min Cheng, Chun Yu, Xiaowei Chen, Mengling Shi, Shuxian Qin, Jing Guo, Zhiqin Shen, Aiguo |
| description | Neuronal nitric oxide synthase (nNOS) has been implicated to influence peripheral nerve lesion and regeneration. Post-synaptic density-95 (PSD-95) is one of nNOS-anchoring proteins and plays an important role in specifying the sites of reaction of NO in nervous system. Here we established a rat sciatic nerve crush (SNC) model to examine the spatiotemporal expression of PSD-95 and nNOS. At gene levels, PSD-95 mRNA diminished shortly after crush, and significantly elevated from 2 days to 2 weeks, whereas nNOS decreased progressively post-operation, reached the valley at 1 day, and markedly up-regulated from 1 to 2 weeks after SNC. The expression of both molecules returned to the control level at 4 weeks post-injury. At protein levels, PSD-95 and nNOS underwent the similar changes as their gene expression except for a time lag during up-regulating. At their peak expression, PSD-95 co-labeled with nNOS in Schwann cells (SCs) of sciatic nerve within 0.5 mm from the lesion site, but had few colocalization
in
axons. In addition, the interaction between PSD-95 and nNOS enhanced significantly at 2 weeks after SNC. These results suggest a correlation of PSD-95 up-regulation with nNOS in reactive SCs of crushed sciatic nerve, which may lead to understanding the function of PSD-95 during peripheral nerve regeneration. |
| doi_str_mv | 10.1007/s11064-007-9555-y |
| format | Article |
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in
axons. In addition, the interaction between PSD-95 and nNOS enhanced significantly at 2 weeks after SNC. These results suggest a correlation of PSD-95 up-regulation with nNOS in reactive SCs of crushed sciatic nerve, which may lead to understanding the function of PSD-95 during peripheral nerve regeneration.</description><identifier>ISSN: 0364-3190</identifier><identifier>EISSN: 1573-6903</identifier><identifier>DOI: 10.1007/s11064-007-9555-y</identifier><identifier>PMID: 18095156</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Animals ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Cell Biology ; Disks Large Homolog 4 Protein ; Female ; Intracellular Signaling Peptides and Proteins - genetics ; Intracellular Signaling Peptides and Proteins - metabolism ; Membrane Proteins - genetics ; Membrane Proteins - metabolism ; Nerve Crush ; Nerve Regeneration ; Neurochemistry ; Neurology ; Neurosciences ; Nitric Oxide Synthase Type I - genetics ; Nitric Oxide Synthase Type I - metabolism ; Original Paper ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Sciatic Nerve - cytology ; Sciatic Nerve - injuries ; Sciatic Nerve - metabolism</subject><ispartof>Neurochemical research, 2008-06, Vol.33 (6), p.1090-1100</ispartof><rights>Springer Science+Business Media, LLC 2007</rights><rights>Springer Science+Business Media, LLC 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c497t-96cb957216bac056b8702d066e3a3a758455092a215db9b349d62edfc428abf3</citedby><cites>FETCH-LOGICAL-c497t-96cb957216bac056b8702d066e3a3a758455092a215db9b349d62edfc428abf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11064-007-9555-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11064-007-9555-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18095156$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gao, Shangfeng</creatorcontrib><creatorcontrib>Fei, Min</creatorcontrib><creatorcontrib>Cheng, Chun</creatorcontrib><creatorcontrib>Yu, Xiaowei</creatorcontrib><creatorcontrib>Chen, Mengling</creatorcontrib><creatorcontrib>Shi, Shuxian</creatorcontrib><creatorcontrib>Qin, Jing</creatorcontrib><creatorcontrib>Guo, Zhiqin</creatorcontrib><creatorcontrib>Shen, Aiguo</creatorcontrib><title>Spatiotemporal Expression of PSD-95 and nNOS After Rat Sciatic Nerve Injury</title><title>Neurochemical research</title><addtitle>Neurochem Res</addtitle><addtitle>Neurochem Res</addtitle><description>Neuronal nitric oxide synthase (nNOS) has been implicated to influence peripheral nerve lesion and regeneration. Post-synaptic density-95 (PSD-95) is one of nNOS-anchoring proteins and plays an important role in specifying the sites of reaction of NO in nervous system. Here we established a rat sciatic nerve crush (SNC) model to examine the spatiotemporal expression of PSD-95 and nNOS. At gene levels, PSD-95 mRNA diminished shortly after crush, and significantly elevated from 2 days to 2 weeks, whereas nNOS decreased progressively post-operation, reached the valley at 1 day, and markedly up-regulated from 1 to 2 weeks after SNC. The expression of both molecules returned to the control level at 4 weeks post-injury. At protein levels, PSD-95 and nNOS underwent the similar changes as their gene expression except for a time lag during up-regulating. At their peak expression, PSD-95 co-labeled with nNOS in Schwann cells (SCs) of sciatic nerve within 0.5 mm from the lesion site, but had few colocalization
in
axons. In addition, the interaction between PSD-95 and nNOS enhanced significantly at 2 weeks after SNC. These results suggest a correlation of PSD-95 up-regulation with nNOS in reactive SCs of crushed sciatic nerve, which may lead to understanding the function of PSD-95 during peripheral nerve regeneration.</description><subject>Animals</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Biology</subject><subject>Disks Large Homolog 4 Protein</subject><subject>Female</subject><subject>Intracellular Signaling Peptides and Proteins - genetics</subject><subject>Intracellular Signaling Peptides and Proteins - metabolism</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - metabolism</subject><subject>Nerve Crush</subject><subject>Nerve Regeneration</subject><subject>Neurochemistry</subject><subject>Neurology</subject><subject>Neurosciences</subject><subject>Nitric Oxide Synthase Type I - genetics</subject><subject>Nitric Oxide Synthase Type I - metabolism</subject><subject>Original Paper</subject><subject>Random Allocation</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Sciatic Nerve - 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genetics</topic><topic>Intracellular Signaling Peptides and Proteins - metabolism</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Proteins - metabolism</topic><topic>Nerve Crush</topic><topic>Nerve Regeneration</topic><topic>Neurochemistry</topic><topic>Neurology</topic><topic>Neurosciences</topic><topic>Nitric Oxide Synthase Type I - genetics</topic><topic>Nitric Oxide Synthase Type I - metabolism</topic><topic>Original Paper</topic><topic>Random Allocation</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Sciatic Nerve - cytology</topic><topic>Sciatic Nerve - injuries</topic><topic>Sciatic Nerve - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gao, Shangfeng</creatorcontrib><creatorcontrib>Fei, Min</creatorcontrib><creatorcontrib>Cheng, Chun</creatorcontrib><creatorcontrib>Yu, Xiaowei</creatorcontrib><creatorcontrib>Chen, Mengling</creatorcontrib><creatorcontrib>Shi, Shuxian</creatorcontrib><creatorcontrib>Qin, Jing</creatorcontrib><creatorcontrib>Guo, Zhiqin</creatorcontrib><creatorcontrib>Shen, Aiguo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Neurochemical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gao, Shangfeng</au><au>Fei, Min</au><au>Cheng, Chun</au><au>Yu, Xiaowei</au><au>Chen, Mengling</au><au>Shi, Shuxian</au><au>Qin, Jing</au><au>Guo, Zhiqin</au><au>Shen, Aiguo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Spatiotemporal Expression of PSD-95 and nNOS After Rat Sciatic Nerve Injury</atitle><jtitle>Neurochemical research</jtitle><stitle>Neurochem Res</stitle><addtitle>Neurochem Res</addtitle><date>2008-06-01</date><risdate>2008</risdate><volume>33</volume><issue>6</issue><spage>1090</spage><epage>1100</epage><pages>1090-1100</pages><issn>0364-3190</issn><eissn>1573-6903</eissn><abstract>Neuronal nitric oxide synthase (nNOS) has been implicated to influence peripheral nerve lesion and regeneration. Post-synaptic density-95 (PSD-95) is one of nNOS-anchoring proteins and plays an important role in specifying the sites of reaction of NO in nervous system. Here we established a rat sciatic nerve crush (SNC) model to examine the spatiotemporal expression of PSD-95 and nNOS. At gene levels, PSD-95 mRNA diminished shortly after crush, and significantly elevated from 2 days to 2 weeks, whereas nNOS decreased progressively post-operation, reached the valley at 1 day, and markedly up-regulated from 1 to 2 weeks after SNC. The expression of both molecules returned to the control level at 4 weeks post-injury. At protein levels, PSD-95 and nNOS underwent the similar changes as their gene expression except for a time lag during up-regulating. At their peak expression, PSD-95 co-labeled with nNOS in Schwann cells (SCs) of sciatic nerve within 0.5 mm from the lesion site, but had few colocalization
in
axons. In addition, the interaction between PSD-95 and nNOS enhanced significantly at 2 weeks after SNC. These results suggest a correlation of PSD-95 up-regulation with nNOS in reactive SCs of crushed sciatic nerve, which may lead to understanding the function of PSD-95 during peripheral nerve regeneration.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>18095156</pmid><doi>10.1007/s11064-007-9555-y</doi><tpages>11</tpages></addata></record> |
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| subjects | Animals Biochemistry Biomedical and Life Sciences Biomedicine Cell Biology Disks Large Homolog 4 Protein Female Intracellular Signaling Peptides and Proteins - genetics Intracellular Signaling Peptides and Proteins - metabolism Membrane Proteins - genetics Membrane Proteins - metabolism Nerve Crush Nerve Regeneration Neurochemistry Neurology Neurosciences Nitric Oxide Synthase Type I - genetics Nitric Oxide Synthase Type I - metabolism Original Paper Random Allocation Rats Rats, Sprague-Dawley Sciatic Nerve - cytology Sciatic Nerve - injuries Sciatic Nerve - metabolism |
| title | Spatiotemporal Expression of PSD-95 and nNOS After Rat Sciatic Nerve Injury |
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